48 results about "Rhodamine 6G" patented technology

The invention belongs to an advanced material technology, and relates to a novel rhodamine derivative, in particular to a 2-aminothiazolyl rhodamine 6G derivative as well as a preparation method and application thereof. Rhodamine 6G and 2-aminothiazole are used as raw materials, the 2-aminothiazolyl rhodamine 6G derivative RGAT is prepared through a one-step reaction, on one hand, RGAT shows excellent selectivity and very high sensitivity to Fe < 3 + >, fluorescence enhancement is remarkable, the linear range of Fe < 3 + > is wide, in the Fe < 3 + > concentration range of 0-50 M, a good linear relation with the Fe < 3 + > concentration is shown, and the detection limit is as low as 2.4 nM; on the other hand, the RGAT has better antibacterial performance on gram-positive bacteria and gram-negative bacteria than commercially available bleaching water, and the RGAT is very low in cytotoxicity, so that the RGAT has a good application prospect in the fields of environmental monitoring and biological medicine.

The invention discloses Cu / CuO / Ag nano-particles and a preparation method and application thereof, and the method successfully prepares the Cu / CuO / Ag nano-particles with regular morphology and uniform dispersion by optimizing a dual-stabilizer system and a step-by-step reduction-replacement process. The material has dual functions of SERS enhancement and catalysis: as an SERS substrate, the enhancement factor of rhodamine 6G reaches 5.3 * 10 < 8 >, the detection limit is as low as 5.0 * 10 <-13 > mol / L, and the reproducibility is good (RSD = 6.2%); meanwhile, as a catalyst for hydrazinolysis and reduction reaction, by optimizing a reaction solvent and the pH value of a system, the reaction efficiency is remarkably improved, and an additional incubation step is not needed. The detection limit on the plasticizer DOTP can reach 2.0 * 10 <-9 > mol / L, and the method has the advantages of simplicity and convenience in operation, good reproducibility, high analysis speed and the like, and is suitable for rapid screening and quantitative analysis of plasticizers in food packaging, environmental water bodies and medical instruments.

The application relates to a surface enhanced Raman detection technology, in particular to a spherical composite SERS substrate and a preparation method and application thereof. The method comprises the following steps: adopting a micro flow injection pump to drop a solution containing silver nanowires and gold nanorods into liquid nitrogen, and then performing freeze drying; wherein, a gun head of the micro flow injection pump is a hydrophobic gun head; the concentration of the silver nanowires in the solution containing the silver nanowires and the gold nanorods is 15-40 mg / mL. The spherical composite SERS substrate prepared by the method has a spherical structure formed by silver nanowires and metal nanorods, the spherical skeleton structure is formed by interweaving silver nanowires into a net, and the metal nanorods are loaded on the silver nanowires, and the spherical composite SERS substrate can realize detection of rhodamine 6G without introducing a modifier.

The application discloses a porous silver nanosheet / zinc oxide nanorod array structure, a preparation method and application thereof. The zinc oxide nanorod is vertically grown on a conductive substrate, and then a layer of gold is decorated on the zinc oxide nanorod and the surface of the substrate where the zinc oxide nanorod is located; and porous silver nanosheets are grown in the gaps between the surface of the gold-plated zinc oxide nanorod and the gold-plated zinc oxide nanorod. The preparation method of the porous silver nanosheet / zinc oxide nanorod array structure is as follows: under the condition of a 90-98 DEG C water bath, zinc oxide nanorod arrays are grown on the surface of a conductive glass placed in a zinc-ammonia solution; then a small amount of gold is decorated on the surface of the zinc oxide nanorod by using a sputtering method; and finally, porous silver nanosheets are electrodeposited on the surface of the zinc oxide nanorod array by using an alkaline silver electrolyte, so as to obtain the target product. The product has high surface-enhanced Raman scattering (SERS) sensitivity, and can be widely applied to SERS detection of organic matters, such as rapid detection of rhodamine 6G.

The invention discloses a porphyrin-Fe < 3 + > complex nanowire as well as a preparation method and application thereof. The nanowire is prepared by the following steps: dissolving FeCl3. 6H2O and 5, 10, 15, 20-tetra (4-carboxyl phenyl) porphin into a mixed solvent of absolute ethyl alcohol and N, N-dimethylformamide, and carrying out ultrasonic treatment to obtain a mixed solution; performing solvothermal reaction on the mixed solution in a high-pressure reaction kettle, naturally cooling to room temperature after the reaction is finished, and performing centrifugal separation to obtain a solid product, namely the porphyrin-Fe < 3 + > complex nanowire. The porphyrin-Fe < 3 + > complex nanowire provided by the invention has the advantages of large specific surface area, better conductivity and rich carboxyl groups, monodisperse metal nodes and delocalized macrocyclic conjugated structures on the surface, and can be used for photocatalytic degradation of rhodamine 6G dye.

The invention discloses a baijiu detection method based on a colorimetric and fluorescent dual-signal sensing array, and relates to the technical field of substance detection and analysis. According to the detection method, the principle that rhodamine B and rhodamine 6G have remarkable ultraviolet-visible spectrums and fluorescence characteristic peaks, and rhodamine B and rhodamine 6G can be accelerated to be catalytically degraded by MnONSs under the acidic condition is utilized, so that optical signals (colorimetric-fluorescence double signals) of rhodamine B and rhodamine 6G can be adjusted by adjusting the catalytic action of MnONSs; according to the method, a colorimetric and fluorescent dual-signal sensing array is constructed, then the dual-signal sensing array is utilized to form characteristic fingerprints of various Baijiu samples, rapid and accurate detection of Baijiu is achieved, and the method is few in types of detection materials, simple and convenient to operate and more suitable for large-scale popularization and application.

The application belongs to the field of organic chemistry, material science and corrosion engineering, and discloses synthesis and application of a corrosion inhibitor based on rhodamine 6G. The fluorescent corrosion inhibitor LDS is obtained by synthesizing rhodamine 6G and hydrazine hydrate through a Schiff base reaction and salicylaldehyde. The corrosion inhibitor LDS not only has an inhibiting effect on carbon steel, but also can realize detection functions of corrosion iron ions and environment pH through a fluorescent signal, and further early warning of metal corrosion.

The present invention relates to the use of a probe, in particular chosen from rhodamine or a derivative thereof, for detecting at least one molecule responsible for boar odor, such as indole or skatole, said probe being chosen, for example, from the group consisting of rhodamine 6G, eosin Y, rhodamine B and rhodamine 101.

This invention relates to the field of X-ray imaging technology and discloses an X-ray imaging scintillator screen based on chiral perovskite nanocrystals and its fabrication process. The screen comprises: a flexible transparent substrate made of polyethylene terephthalate or polyimide film; a chiral perovskite nanocrystal composite layer; an inorganic oxide protective layer composed of SiO₂ or Al₂O₃; and a composite reflective layer comprising: a TiO₂ nanorod array layer located on the substrate surface; and a reflectance enhancement layer doped with 1-10 vol% rhodamine 6G or coumarin derivatives covering the surface of the TiO₂ nanorod array layer. By using a high-transmittance, low-roughness polyester or polyimide material on the flexible transparent substrate, combined with the surface-grown TiO₂ nanorod array and chiral perovskite nanocrystal composite layer, the light reflection efficiency and imaging contrast are significantly improved. Furthermore, the optimized light extraction path, achieved through a microprism structure, enhances the efficiency of X-ray conversion to visible light.

The invention discloses a carbon dot with high fluorescence quantum yield and narrow bandwidth emission, which is prepared by taking citric acid and rhodamine 6G as carbon sources for hydrothermal reaction, and emits orange fluorescence under ultraviolet excitation, the emission wavelength is 556 + / -2nm, the fluorescence quantum yield is not less than 80%, the half-peak width is not more than 50nm, and the narrow bandwidth characteristic of the carbon dot can effectively improve the light color purity. And when being applied to preparation of a high-color-purity photoluminescent diode as photoconversion fluorescent powder, the fluorescent powder shows excellent optical performance.

The present invention belongs to the field of advanced materials technology, and relates to a new rhodamine derivative, specifically to a 2-aminothiazolyl rhodamine 6G derivative and its preparation method and application. The present invention uses rhodamine 6G and 2-aminothiazole as raw materials to prepare the 2-aminothiazolyl rhodamine 6G derivative RGAT in a one-step reaction. On the one hand, RGAT shows excellent selectivity and high sensitivity to Fe<supgt;3+< / supgt>, with a significant fluorescence enhancement, a wide linear range for Fe<supgt;3+< / supgt>, and a good linear relationship with the Fe<supgt;3+< / supgt> concentration in the range of 0-50 µM of Fe<supgt;3+< / supgt> concentration, and the detection limit is as low as 2.4 nM. On the other hand, RGAT has antibacterial properties superior to commercially available bleach against both Gram-positive and Gram-negative bacteria, and RGAT has very low cytotoxicity, showing good application prospects in the fields of environmental monitoring and biomedicine.

This invention relates to a method for preparing and applying an ordered silver nanoparticle@bismuth nanosheet@copper mesh array. First, bismuth nanosheets are grown on the surface of a copper mesh through an etching reaction using an immersion method. Then, silver nanoparticles are sputtered onto the surface of the bismuth nanosheets, resulting in an ordered silver nanoparticle@bismuth nanosheet@copper mesh array film. This array film is then immersed in a 4-p-aminobenzaldehyde (4-ABZ) solution, adsorbing 4-ABZ molecules onto the surface of the silver nanoparticle@bismuth nanosheet@copper mesh array as signal probe molecules. The silver nanoparticle@bismuth nanosheet@copper mesh array with adsorbed 4-ABZ molecules is then placed as a substrate in a packaging environment containing seafood, meat, or other meat-derived foods. The SERS signal intensity of the 4-ABZ molecules on the substrate surface is used to monitor the freshness of the meat-derived foods. As the freshness of the meat-derived foods decreases, the SERS signal of the 4-ABZ molecules on the substrate surface gradually decreases. Meanwhile, the silver nanoparticle@bismuth nanosheet@copper mesh array also exhibits excellent SERS signal response and signal uniformity for organic dye pollutant molecules such as rhodamine 6G and 4-aminobenzylthiophenol (4-ATP), and has important application prospects in the field of environmental monitoring.

This invention relates to the field of salicornin detection technology, and particularly to a dual-color visualization analysis and detection method for salicornin. The method includes: taking a shellfish sample to be tested, adding hydrochloric acid solution, boiling and stirring, and centrifuging at low temperature; collecting the supernatant and adjusting the pH of the supernatant to <4 with hydrochloric acid solution; adding PBS solution and mixing well; adding the test solution to a suspension of MNP@Ag-Apt-cDNA-Au / Pt signal probe and incubating at room temperature; magnetic separation, taking the supernatant and adding it to 4-7 times its volume of dual-color development solution, and incubating for 10-20 min; the dual-color development solution includes 1 mM rhodamine 6G, 0.6 mM TMB, and 1 mM hydrogen peroxide in a volume ratio of 0.3-1:10:10; taking images with a camera, extracting the RGB histogram data, analyzing the data, and determining the concentration of salicornin. The advantages are low detection limit and high sensitivity.

The invention relates to the technical field of high-sensitivity trace substance detection, in particular to a gold particle transfer method for preparing a PDMS (polydimethylsiloxane) flexible substrate, which comprises the following steps: step 1, preparing a gold nanoparticle solution; as the ethanol is injected into the gold nanoparticle solution, a layer of gold nanoparticle film is gradually formed on a water-oil interface; and step 3, preparing the flexible SERS substrate. According to the method, the gold nanoparticles can be successfully transferred to the flexible substrate; naturally settling a gold particle film formed by liquid-liquid self-assembly on the surface of PDMS in an original compact arrangement mode; the substrate prepared by the preparation method has a good detection effect on an object to be analyzed, rhodamine 6G is used as a Raman active probe, and the limit can be measured to be 1 * 10 <-8 > mol / L.

The invention relates to the technical field of spectrum sensing, in particular to a single-layer V-doped WS2 defect regulation type surface enhanced Raman scattering (SERS) substrate, a preparation method of the single-layer V-doped WS2 defect regulation type SERS substrate, and an application of the single-layer V-doped WS2 defect regulation type SERS substrate. The preparation method comprises the following steps: firstly, preparing single-layer V-doped WS2 by adopting a chemical vapor deposition (CVD) method, specifically comprising the following steps: mixing a tungsten-containing precursor and a vanadium-containing precursor according to different proportions, putting the mixture and a sulfur precursor into a double-temperature-zone tubular furnace, and reacting the tungsten-containing precursor and the vanadium-containing precursor in a high-temperature inert gas environment to prepare the single-layer V-doped WS2 single crystal on a growth substrate. By comparing the influence of different V doping concentrations on the SERS performance of the single-layer WS2, the SERS performance of the corresponding single-layer V-doped WS2 is optimal when the molar ratio of V to W in the current driving body is 4: 1 and the V doping concentration reaches 8.38 at.%, the detection concentration limit of rhodamine 6G molecules reaches 10 <-10 > M (mol / L), the enhancement factor reaches 10 < 6 >, and the SERS detection capability is improved by 6 orders of magnitude compared with that of the single-layer non-doped WS2.

The invention provides a green synthesis method of an alpha-aminoketone compound based on visible light catalytic oxidation amination reaction, and belongs to the technical field of organic chemical synthesizing.According to the method, aliphatic aldehyde and secondary amine which are low in price and easy to obtain serve as raw materials, oxygen serves as a green terminal oxidizing agent, and the alpha-aminoketone compound is synthesized under normal temperature and visible light irradiation. The alpha-aminoketone compound is efficiently synthesized in one step through oxidative amination reaction catalyzed by organic photosensitizers such as rhodamine 6G and anthraquinone. According to the method, a noble metal catalyst and a stoichiometric oxidant are abandoned, the reaction condition is mild (room temperature), the operation is simple and convenient, the substrate application range is wide, and the method shows excellent compatibility to functional groups including various complex cyclic secondary amines (such as natural product derivatives of nornicotine, cytisine and the like); compared with the prior art, the method has the advantages that important transformation from thermal activation to light activation is realized on the reaction mechanism, and a novel green way which is more economical and environment-friendly and is easy for large-scale production is provided for synthesis of alpha-aminoketone.

The invention belongs to the technical field of surface enhanced Raman scattering (SERS) detection, and discloses a NiCo2S4-MoS2 composite material with a Raman enhancement effect as well as a preparation method and application of the NiCo2S4-MoS2 composite material. The NiCo2S4-MoS2 composite material is prepared by the following steps: adding a precursor of Mo, CH4N2S and a reducing agent into deionized water to obtain a mixed solution; and adding a precursor of Ni, a precursor of Co and a complexing agent into the mixed solution, performing ultrasonic treatment, performing hydrothermal reaction at 100-250 DEG C, centrifuging, cleaning and performing vacuum drying, thereby obtaining the Ni-Co composite material. The NiCo2S4-MoS2 composite material has a Raman enhancement effect, has rich sulfur vacancies, can be applied to analysis and detection of trace organic matters, has a relatively strong surface Raman enhancement effect on rhodamine 6G, has a detection range of 10 <-5 >-10 <-9 > mol / L, and has a relatively good application prospect.

The invention discloses a rhodamine-based pH fluorescent probe as well as a preparation method and application thereof. According to the invention, a trans-1, 4-cyclohexanediamine group is introduced on a rhodamine 6G molecule through an amine ester condensation reaction, so that regulation and control on the acid response performance of the rhodamine-based pH fluorescent probe are realized. Researches show that pKa of the probe is greatly improved through modification of trans-1, 4-cyclohexanediamine, detection of the probe on a weak acid solution (pH is 5-7) is achieved, the acid response speed is increased, and the weak acid solution can be rapidly detected through color and fluorescence double channels. On the basis, coupling probe degreasing cotton is prepared through oxidation of carriers such as degreasing cotton and an aldehyde amine condensation reaction, and the coupling probe degreasing cotton can be used for monitoring and imaging gastric juice leakage in an operation to prevent occurrence of postoperative gastric fistula.

The invention discloses application of ornidazole test paper based on a fluorescent composite material. A stable yellow light emission fluorescent dye rhodamine 6G is introduced into a nano pore channel of a high-stability UIO-67 material to prepare the high-stability yellow light emission R6G-UIO-67 fluorescent composite material, and the fluorescent composite material can efficiently and rapidly detect ornidazole; meanwhile, the production cost is low, the operation is simple, and the luminous efficiency is good. Loading the composite material on filter paper to prepare ornidazole detection test paper; the sample to be detected is dropped on the test paper, whether ornidazole exists or not and the content of ornidazole can be visually judged according to the color change and color depth of the test paper under the condition of ultraviolet lamp irradiation, operation is efficient, and observation is convenient. Therefore, the method for detecting ornidazole by using the test paper is safe, energy-saving, short in detection time, accurate in detection result and visual, and can be popularized and applied on a large scale in ornidazole detection.

The invention discloses an Au-coated Ag / C-CNF / PEGDA hydrogel SERS (Surface Enhanced Raman Scattering) sensor as well as a preparation method and application thereof, and belongs to the technical field of analysis and detection. Gold and silver nanoparticles with a core-shell structure, polyethylene glycol diacrylate and carboxylated cellulose react to form an Au-coated Ag / C-CNF / PEGDA hydrogel prepolymer solution, and the hydrogel prepolymer solution is subjected to ultraviolet crosslinking under the action of a photoinitiator to form the porous three-dimensional network structure Au-coated Ag / C-CNF / PEGDA hydrogel SERS sensor with a synergistic effect. The hydrogel sensor provided by the invention not only can accurately detect 10 <-9 > M-10 <-5 > M Raman signal molecule rhodamine 6G (R6G), but also has good sensitivity and excellent uniformity, the RSD is as low as 4.92%, and the detection limits on anthracycline antitumor drugs doxorubicin (DOX) and mitoxantrone (MTO) respectively reach 3.4 * 10 <-8 > M and 3.6 * 10 <-9 > M.

The invention discloses a polyurethane elastomer material with multi-stimulus response discoloration, high mechanical strength and self-healing characteristics as well as a preparation method and application thereof, in the structural formula of the polyurethane elastomer material, A is selected from substituted or unsubstituted phenyl, biphenyl, xylyl, naphthyl, cyclohexyl, butyl and hexyl, and B is selected from substituted or unsubstituted phenyl, biphenyl, xylyl, naphthyl, cyclohexyl, butyl and hexyl; the substituent groups are alkyl groups, alkoxy groups and halogen methyl groups of C4-C6; and B is polyether polyol. W is a positive integer ranging from 10 to 80, x is a positive integer ranging from 4 to 8, y is a positive integer ranging from 20 to 70, and z is a positive integer ranging from 20 to 90. According to the invention, adipic acid dihydrazide, 2, 2-bis (hydroxymethyl) propionic acid and rhodamine 6G ethylene glycol are used as chain extenders, and organic tin is used for catalytic polymerization to form the organic tin-based polymer. The material disclosed by the invention has the characteristic of color change response to external stress, ultraviolet irradiation and an acid environment, can be subjected to shape programming after being heated, and also has excellent mechanical strength and self-healing performance.

The application discloses a method for packaging fluorophores based on tris-dolene nanofilm. First, a nanofilm with tris-dolene as a core segment is prepared through gas-liquid interface assembly and condensation reaction, and is carried on a quartz glass. In a wet state, the pore structure of the nanofilm can be maintained without collapsing through freeze-drying treatment. Then, a fluorophore solution is added dropwise on the nanofilm. After the solvent is naturally evaporated, the fluorophore is uniformly dispersed in the nanofilm, spatial and electronic isolation of the fluorophore is realized, and a flexible and uniform fluorescent nanofilm is obtained. The fluorophore packaged by the method includes coumarin (such as 7-(diethylamino) coumarin-3-carbonitrile and coumarin 6), acridine (such as acridine orange), xanthene (such as rhodamine 6G and rhodamine B) and quinonimine (such as Nile red), etc. In addition, the optical properties and sensing behavior of the obtained fluorescent nanofilm are consistent with the optical properties and sensing behavior of the fluorophore in a solution state. Therefore, the application has universality and fidelity.

The invention provides a nano-enzyme synthesis method which comprises the following specific steps: sequentially heating, drying and grinding an aqueous solution containing 0-generation polyamide-amine dendritic macromolecules, potassium ferricyanide and urea, carrying out high-temperature pyrolysis twice, and grinding again to obtain the target nano-enzyme. The invention also provides a corresponding dichlorvos detection method, which comprises the following operation steps: mixing a sample solution, a buffer solution and a nano-enzyme solution for incubation, and then adding the mixed solution into a borate buffer solution containing hydrogen peroxide for reaction; and after the reaction is finished, sequentially adding a potassium iodide solution and a rhodamine 6G solution, and carrying out fluorescence detection after the reaction is finished. The nano-enzyme synthesized by the method has multiple enzymatic characteristics of oxidase-like, peroxidase-like, catalase-like and superoxide dismutase-like, has the advantages of excellent selectivity and high sensitivity in detection of dichlorvos, and is suitable for rapid detection of dichlorvos in actual samples.

This invention belongs to the field of surface-enhanced Raman scattering (SERS) detection technology, and relates to the application of a silver-cobaltate composite SERS substrate in the detection of Rhodamine 6G. First, cobaltate materials are prepared via a hydrothermal method using cobalt and bismuth sources. Then, silver nanoparticles are prepared by ascorbic acid reduction of silver nitrate and loaded onto the cobaltate surface to obtain the silver-cobaltate composite SERS substrate. This substrate effectively improves the dispersion state of the silver nanoparticles, increases the contact opportunity between the target molecule and the active site, and enhances the Raman response intensity. Testing with Rhodamine 6G as the probe molecule shows that the prepared silver-cobaltate composite SERS substrate exhibits excellent enhancement effect, good uniformity, and repeatability, and can be used for the rapid detection and analysis of trace organic dyes, demonstrating good application value in environmental monitoring and chemical detection fields.

The invention discloses a flexible SERS (Surface Enhanced Raman Scattering) chip, a preparation method of the flexible SERS chip and application of the flexible SERS chip in detection of marine food pollutants, and belongs to the technical field of analytical testing. The preparation method comprises the following steps: preparing carbon dot-silver nanoparticle composite sol through one-pot in-situ reduction; pretreating a filter paper substrate; loading the composite sol on the surface of filter paper, and drying and curing. The capillary enrichment effect of the filter paper, the electromagnetic enhancement effect of the silver nanoparticles and the charge transfer chemical enhancement effect of the carbon dot-silver nanoparticle heterogeneous interface are utilized to construct an enrichment-electromagnetic enhancement-chemical enhancement triple synergistic mechanism, and the SERS detection sensitivity, the signal uniformity and the substrate stability are remarkably improved. The preparation method is simple and low in cost, the detection limit of the prepared chip to rhodamine 6G is as low as 10-15 M, the enhancement factor is as high as 1013, in-situ'wiping-detection 'of crystal violet pollutants on the surface of a prawn body can be realized, and the chip has a wide application prospect in the field of rapid screening of a marine food safety site.

The application discloses an artificial light capturing system and a construction method and application thereof, and belongs to the technical field of agricultural light regulation and functional materials, wherein the artificial light capturing system comprises a donor and an acceptor, the donor is a complex formed by a compound DBI and a metal cation, and the acceptor is a fluorescent dye capable of receiving the energy of the donor and emitting fluorescence. The application constructs the artificial light capturing system by taking the complex formed by the small-molecule compound DBI and the metal cation as the donor and taking rhodamine 6G as the acceptor, and has the characteristics of simple synthesis, low cost, excellent light conversion capacity (converting short-wavelength light into long-wavelength light) and adjustable donor-acceptor ratio; the artificial light capturing system is used for preparing a light conversion film, and can promote the growth of legume plants such as Dutch beans.

The invention discloses a multifunctional rhodamine 6G derivative connected with 2-thiazole ethylamine and a preparation method and application of the multifunctional rhodamine 6G derivative connected with 2-thiazole ethylamine, rhodamine 6G and 2-thiazole ethylamine are used as raw materials, and the multifunctional rhodamine 6G derivative connected with 2-thiazole ethylamine is prepared through one-step reaction. According to the invention, a novel multifunctional rhodamine 6G derivative RGET is synthesized through a one-step method. RGET can be used as a high-selectivity and high-sensitivity Fe < 3 + > spectrum probe, the Fe < 3 + > detection limit is 2.1 nM, and Fe < 3 + > enables fluorescence to be enhanced by 541 times; meanwhile, RGET has antibacterial performance comparable to that of commercially available bleaching water for gram-positive bacteria and gram-negative bacteria, has very low cytotoxicity, and has bright application prospects in the fields of environment and life science.

Fluorescence titration of methylene blue, rhodamine B and rhodamine 6G (R6G) by silver nanoparticle (AgNP) all resulted in an initial steep quenching curve followed with a sharp turn and a much flatter quenching curve. At the turn, there are about 200,000 dye molecules per a single AgNP, signifying self-assembly of approximately 36 layers of dye molecules on the surface of the AgNP to form a micelle-like structure. These fluorescence-quenching curves fit to a mathematical model with an exponential term due to molecular self-assembly on a AgNP surface, or “self-assembly shielding effect”, and a Stern-Volmer term (nanoparticle surface enhanced quenching). Such a “super-quenching” by AgNP can only be attributed to “pre-concentration” of the dye molecules on the nanoparticle surface that yields the formation of micelle-like self-assembly, resulting in great fluorescence quenching. Overall, the fluorescence quenching titration reveals three different types of interactions of dye molecules on AgNP surface: 1) self-assembly (methylene blue, rhodamine B and R6G), 2) absorption / tight interaction (tryptamine and fluorescein), and 3) loose interaction (eosin Y). We attribute the formation of micelle-like self-assembly of these three dye molecules on AgNP to their positive charge, possession of nitrogen atoms, and with relatively large and flat aromatic moieties.

The invention relates to the technical field of analytical chemistry, and discloses a single-column separation and analysis method of rhodamine 6G and a precursor thereof, a methanol-ammonium formate buffer salt mixed solution is adopted as a sample solvent and a mobile phase core component, and the single-column separation and analysis method of the rhodamine 6G and the precursor thereof is established according to the polarity difference of compounds at different reaction stages. Optimized isocratic and gradient elution procedures are respectively adopted on a single chromatographic column, so that the problems that strong polar compounds are insufficient in retention, a chromatographic system is easy to block and multiple components cannot be synchronously and quickly separated are effectively solved. Through the arrangement, efficient, stable and synchronous separation and quantification of key reaction intermediates and products in the rhodamine 6G production process are realized, and reliable analysis guarantee is provided for dynamic monitoring of the production process and product quality control.