210 results about "Rhodamine 6G" patented technology

The invention relates to a piece of surface enhanced raman detection test paper and the application thereof, which belong to the technical field of ultra-sensitive test analysis, in particular to the piece of surface enhanced raman detection test paper and the application of the paper to detection of biological or chemical probe molecules with raman signals. The surface enhanced raman detection test paper is obtained by covering a precious metal layer on the surface of paper with a natural fiber micro nano multi-grade structure through the physical vapor deposition or chemical plating technology, the paper can be filter paper, parchment paper, napkin, newspaper or printing paper and the like, the precious metal film is gold or silver and the like, and the thickness of the precious metal film is 5 nanometers to 90 nanometers. Combined action of size, period and roughness enables incident light to generate a local electric field on the surface of a multi-stage structure and enables the electric field to be enhanced, and the detection limit can reach 10-10mol/L. The test paper has the advantages of being good in flexibility, low in cost, free of environment pollution, capable of being prepared in batch and the like, thereby being capable of being used in detection of probe molecules such as rhodamine 6G, p-aminothiophenol, riboflavin or ethanol and the like.

The invention discloses a silver nanoparticle-modified zinc oxide nanorod array, a preparation method thereof and application thereof. An array consisting of zinc oxide nanorods is arranged on a substrate; the length of the zinc oxide nanorods is 1 to 1.4mu.m; the diameter of the rods is 50 to 60nm; the size of silver nanoparticles at the top ends of the rods is 100 to 120nm; the size of the silver nanoparticles on the surfaces of the rods is 25 to 35nm; the distance between the particles is less than or equal to 10nm; the distance between the silver nanoparticles at the top ends of the adjacent rods is 40 to 60nm; and the distance between the silver nanoparticles on the surfaces of the adjacent rods is 25 to 35nm. The method comprises the following steps of coating a zinc acetate ethanol solution on the substrate; after drying the substrate, cleaning, dispersing and thermally decomposing the substrate to obtain a substrate on which a zinc oxide seed layer is coated; performing electro-deposition on the substrate coated with the zinc oxide seed layer in a zinc nitrate ammonia complexation solution to obtain a substrate with the zinc oxide nanorod array; and performing silver ion sputtering on the substrate in an ion sputter to obtain the target product. The silver nanoparticle-modified zinc oxide nanorod array can serve as an active substrate of SERS (Surface Enhanced Raman Scattering) and is used for measuring rhodamine 6G or polychlorinated biphenyl 77.

The present invention relates to rhodamine 6G hydrazide salicylal azomethine, a synthesization method and an application in the content determination of cupric ions, which belong to the field of the analysis and determination of cupric ions in water sample. The molecular structural formula of the rhodamine 6G hydrazide salicylal azomethine is shown on the right, and the present invention discloses the synthesization method of the rhodamine 6G hydrazide salicylal azomethine and rhodamine 6G hydrazide salicylal azomethine solution used for the content determination of a small amount of cupric ions in water sample: 0 percent to 98.99 percent of ethanol, 99.99 percent to 1 percent of acetic acid / sodium acetate buffer solution with the pH value of 5 to 9, in which the total concentration of acetic acid is 1 to 100 millimoles per litre, 0.01 percent of N, N-dimethylformamide and 1ppm to 100ppm of rhodamine 6G hydrazide salicylal azomethine, all measured in mass percentage. By a spectrophotometer, the solution can be used for the accurate quantitative determination of 0.005ppm to 0.256ppm of cupric ions in water sample as well as the semiquantitative determination of no less than 0.075ppm of cupric ions in water sample by direct naked-eye observation on the change of colors.

The invention relates to a method for quantitatively detecting trace rhodamine 6G by using a Raman spectrometer, which comprises the following steps of: preparing a plurality of silver nanowire arrays by an alternating current chemical deposition method, namely depositing silver nanowires in holes of an anodic aluminum oxide (AAO) template in a domain limiting mode by using mixed aqueous solution of silver nitrate and boric acid as electrolyte to form the silver nanowire arrays; preparing silver nanoparticles, mixing with rhodamine 6G solution at different concentration respectively, and dripping on the prepared silver nanowire arrays respectively to form a surface enhanced Raman spectroscopy (SERS) enhanced sandwich system; after the sandwich system is naturally dried, performing a Raman spectrum test on the dried sandwich system on the Raman spectrometer to obtain the SERS spectra of the rhodamine 6G solution at the different concentration; calculating the characteristic peak intensity of the SERS spectra of the rhodamine 6G solution at the different concentration to obtain an SERS intensity-concentration relation curve of the rhodamine 6G solution, wherein the curve is taken as a basis of quantitatively detecting the rhodamine 6G; and contrasting the SERS intensity of the rhodamine 6G solution at unknown concentration with the SERS intensity-concentration relation curve of the rhodamine 6G to obtain the concentration of the rhodamine 6G solution.

The invention discloses a preparation method and an application method for a high-sensitivity and high-stability surface-enhanced Raman chip. The method comprises the following steps of: preparing a polystyrene nanosphere array which is distributed in a single layer on a dual-surface-polished quartz substrate of (1 centimeter)*(1 centimeter) through a self-assembly method; etching the prepared single-layer nanosphere array by using a reaction ion etching process to changing the sizes of gaps among nanospheres; and manufacturing a surface-enhanced Raman chip of which the structural period is 430 nanometers, the particle diameter is 160 nanometers and the local surface plasma resonance wavelength is 780 nanometers through silver nano-film deposition and a Lift off process. The surface-enhanced Raman chip disclosed by the invention can be used for detecting a rhodamine 6G molecule of which the concentration is 10nM; and the characteristic peak strength deviation of a Raman spectrum curve of the rhodamine 6G molecule of which the concentration is 10nM at seven different positions on the same surface-enhanced Raman chip with high sensitivity and high stability is +/-3 percent, and the rapid specific detection requirements of biological and chemical substances can be met.

The invention relates to a rhodamine fluorescent probe for detecting Fe <2+>, and a preparation method thereof. The preparation method comprises the following steps: enabling rhodamine 6G and hydrazine hydrate to perform reflux reaction in alcohol to generate rhodamine hydrazide, then enabling rhodamine hydrazide and 2,6-diacetylpyridine to perform reflux reaction in alcohol, and through neutral aluminium trioxide column chromatography purification, obtaining the rhodamine acylhydrazone and acetyl pyridine fluorescent probe. The fluorescent probe is high in synthesis productivity, quick in reaction and high in selectivity, has high specificity to Fe <2+>, can work under the physiological environmental condition with the pH value of 7.4, and has a prospect of being applied to bioluminescence imaging.

The invention relates to a method for preparing surface-enhanced Raman scattering (SERS) substrate by a solution method and application, and belongs to the technical field of SERS substrates. The method comprises the following steps: preparing hydrocarbyl mercaptan modified metal nanoparticles with the diameter of 1 to 5 nm; dissolving the metal nanoparticles with the diameter of 1 to 5 nm into an organic solvent to prepare metal nanoparticle sol of 60 to 100 mg/ml; and coating the metal nanoparticle sol on a substrate, placing a sample coated with a metal colloid film in a precise muffle furnace or a tube furnace, heating to 140 to 250 DEG C within 5 minutes, maintaining for 5 to 30 minutes, and cooling to room temperature. When the SERS substrate obtained by the method is applied to detection of rhodamine 6G and 4-mercaptopyridine, the enhancement factor is 107, and the relative standard difference of Raman signal intensity distribution at different positions is 5%.

The invention provides application of rhodamine 6G ramifications, and relates to the use of the molecule inner spiral ring-containing rhodamine 6G ramifications which are taken as cell lysosome fluorescent coloring agent. The rhodamine 6G ramifications are the molecule inner 5 member ring-containing rhodamine 6G ramifications which can be used as the cell lysosome fluorescent colouring agent to stably mark the cell lysosome at high selectivity and for a long term. The application of the rhodamine 6G ramifications has the beneficial effects that (1) the azyl and molecule inner 5 member ring-containing rhodamine 6G ramifications can be taken as a lysosome fluorescent labeled substance for the cells with different types; (2) the rhodamine 6G ramifications are long in retention time and high in photostability in the cell; and (3) the rhodamine 6G ramifications can be used for labeling and tracking the lysosome in the cell for a long time.

The invention relates to a rhodamine 6G-based mercury ion detection fluorescent probe molecule; the fluorescent probe molecule has a structure as follows. The invention also discloses a preparation method of the rhodamine 6G-based mercury ion detection fluorescent probe molecule, and application thereof in mercury ion detection. The structure is shown in the description.

The invention discloses a film formed by silver nanosheets and a preparation method and application of the film. The film is 200-500nm in thickness and is formed by covering a nanometer flat branch cluster on a conductive substrate, wherein a gap between every two nanometer flat branches in the cluster is 1-15nm; and the film is formed by connecting nanosheets with arc-shaped edges in series, wherein the thickness of each nanosheet is 10-50nm, width is 30-300nm and length is 90-150nm. The preparation method comprises the following steps of: mixing and stirring a silver nitrate water solution, a citric acid water solution and water to obtain a mixed solution, and injecting a sodium borohydride water solution into the mixed solution and stirring and aging to obtain a silver seed crystal solution; then coating the silver seed crystal solution onto the conductive substrate and then airing the conductive substrate; and then putting the conductive substrate which is coated with silver seed crystals and is used as a cathode in electrolyte consisting of the silver nitrate water solution and the citric acid water solution, and carrying out electro-deposition for 5-120 minutes under the constant current of which the current density is 0.1-1mA/cm<2> to obtain the target product. The film disclosed by the invention can be used as an SERS (Surface Enhanced Raman Scattering) active substrate to rapidly detect the content of trace rhodamine 6G or tetrachlorobiphenyl-77 attached onto the SERS active substrate.

The invention discloses an N-(rhodamine 6G) lactam-N'-phenylthiourea derivative. The N-(rhodamine 6G) lactam-N'-phenylthiourea derivative has the chemical structural formula shown as the specifications. The invention also discloses a preparation method of the N-(rhodamine 6G) lactam-N'-phenylthiourea derivative. The preparation method comprises the following steps of: reacting rhodamine 6G serving as a raw material with ammonia to prepare rhodamine 6G lactam; and reacting the rhodamine 6G lactam with phenylisothiocyanate to prepare the N-(rhodamine 6G) lactam-N'-phenylthiourea derivative. A fluorescent probe has the characteristics of increasing dissolution velocity in acetonitrile, enhancing stability of the pH value; stabilizing in acid alkaline environments and the like, and can serves as a probe for a fluorescent indicator of metal ions to detect heavy metal ion content and transition metal ion content of water.

The invention discloses a beta-cyclodextrin/polyethylene imine/graphene oxide composite material, a preparation method thereof, a composition and a detection method. The structure of the beta-cyclodextrin/polyethylene imine/graphene oxide composite material is represented by formula (I) shown in the specification, and n in the formula is a positive integer. The beta-cyclodextrin/polyethylene imine/graphene oxide composite material is synthesized through synthesizing beta-cyclodextrin-polyethylene imine by grafting beta-cyclodextrin to polyethylene imine and through modifying the surface of graphene oxide with beta-cyclodextrin-polyethylene imine. A host-guest competitive inclusion fluorescence recovery process between beta-carotene in the beta-cyclodextrin/polyethylene imine/graphene oxide composite material and a guest molecule rhodamine 6G and beta-carotene carotene is used to detect beta-carotene, so fluorescence process detection of beta-carotene is realized in order to realize simple operation, low detection limit, rapidness and effectiveness.

The invention provides a method for preparing a substrate material with the high-activity surface-enhanced Raman scattering and a silk-screen printing method. According to the method for preparing the substrate material with the high-activity surface-enhanced Raman scattering, carbonate is introduced when silver nanoparticles are prepared, so that obtained silver nanoparticles are irregular, surface-enhanced Raman scattering (SERS) hot points can be generated easily, and the SERS substrate is formed by the synthesized silver nanoparticles in a printing mode. The method is free of product purification, high in preparing speed and visual and is a novel simple, rapid and high-yield synthesis method. According to the SERE substrate prepared with the method, the limit of detection to dye rhodamine 6G can reach 10 <-10> mol/L, the price is low, and traditional expensive silicon substrates can be replaced and supplemented. The method has the advantages that the reaction processes are simple, the reaction time is short, and operation is easy and convenient. The method can be used for industrialized batch preparation and production and can also be used for detection of traditional dye and detection of hazardous additives in food. According to the synthesis method, cleanness and environmental friendliness area achieved, and the method is suitable for application and popularization.

The invention discloses a super-hydrophobic nano-silver raman-enhanced substrate material and a preparation method thereof. The preparation method comprises the following steps: performing surface de-oiling and acid washing on a base material in sequence; performing chemical deposition in chemical silver-plating deposition liquid at 20-80 DEG C for 2-30 minutes; and storing a nano-silver substrate obtained by the deposition for 1-2 months to obtain the super-hydrophobic nano-silver raman-enhanced substrate material. The method is a one-step chemical deposition process and is simple and feasible, and the super-hydrophobic surface with high contact angle hysteresis can be obtained without any surface modification; the prepared substrate has an ultrasensitive raman-enhanced effect, rhodamine 6G is taken as a probe molecule, and the detection limit is reduced to 10-12mol/L; meanwhile, the substrate is very good in surface structure stability and excellent in performances of acid resistance, alkaline resistance, organic solvent resistance, low temperature resistance, high temperature resistance and the like, and can meet the actual application needs.

The present invention discloses a method for preparing a hydrophobic SERS substrate by using a silk-screen printing technology, and belongs to the technical field of SERS substrate preparation. The method comprises: dispersing noble metal nanoparticles in a polymer solution, adding an auxiliary agent, carrying out low temperature ultrasonic dispersion to obtain a uniformly-mixed slurry, placing a pre-treated ITO film or PMMA film onto a flat plate substrate, flatly placing a polyester silk screen onto the ITO film or PMMA film, adding the slurry on the surface of the silk screen in a dropwise manner to print, curing, and cleaning to obtain the hydrophobic SERS substrate. According to the present invention, the reactive thermal curing or ultraviolet curing resin is added so as to make the substrate have the hydrophobicity, such that the sensitivity on the surface-enhanced Raman effect is high; and the detection limit of the prepared SERS substrate on the dye rhodamine 6G can achieve 10<-14> M, and the SERS substrate provides the good Raman enhancement effect on 4-MBA.

The invention provides a nanometer biosensor which is formed by self-assembling of carbon quantum dot-labeled concanavalin and a cholesterol sensor, wherein the cholesterol sensor is prepared by co-incubating cyclodextrin-modified nanogold and rhodamine 6G to remove excessive reactants. Moreover, the invention further provides a preparation method and application of the nanometer biosensor. The nanometer biosensor provided by the invention is established based on a fluorescence resonance energy transfer theory, can synchronously detect glucose and cholesterol, avoids mutual interference in the detection process and is a dual substance quantitative determination sensor which is novel, simple, convenient, low in cost, rapid and high in sensitivity.

The invention discloses a method for preparing a broken-line type inclined silver nanorod array surface enhanced Raman substrate, which belongs to the technical field of a surface enhanced Raman effect. In the invention, an electron beam evaporation film plating machine is utilized, silver is adopted as a target, the broken-line type inclined silver nanorod array with the number of inflection points of 1 to 7 obliquely grows on a silicon-based substrate or a glass-based substrate under the temperature of 200 to 260K, and the broken-line type inclined silver nanorod array surface enhanced Raman substrate is obtained; and then a rhodamine 6G as a detection molecule is utilized for calibrating the surface enhanced Roman capacity of the obtained broken-line type inclined silver nanorod array surface enhanced Raman substrate. The enhanced Roman capacity of the obtained broken-line type inclined silver nanorod array surface enhanced Raman substrate obtained by the invention is obviously stronger than that of a straight-line type inclined silver nanorod array surface enhanced Raman substrate, and is especially stronger when the number of inflection points is 3, 4 or 5; and the method provided by the invention is simple and fast, and is low in cost and high in sensitivity.

The invention belongs to the technical field of preparation of precious metal nano materials and ratiometric fluorescent probes, and particularly relates to a preparation method of a melamine ratiometric fluorescent probe based on silver nanocluster and rhodamine 6G compounds with stable DNA (deoxyribonucleic acid). Electrostatic self-assembly is adopted for constructing a silver nanocluster/rhodamine 6G compound; melamine and thymine in the surface DNA of the silver nanocluster form a hydrogen bond to dissociate rhodamine 6G from the surface of the silver nanocluster, fluorescence resonance energy transfer is destroyed, and silver nanocluster fluorescence is recovered. The process has a slight impact on rhodamine 6G fluorescence, which serves as a reference signal, and silver nanoclusterfluorescence serves as a response signal. A linear relation between a ratio of a silver nanocluster to rhodamine 6G fluorescence emission peak intensity and a melamine molar concentration is fit to construct the melamine ratiometric fluorescent probe. The probe is simple in preparation process, low in cost and high in product sensitivity, and can develop into a novel melamine ratiometric fluorescent probe used for effectively detecting melamine in practical samples.

The invention discloses a mesoporous silicon dioxide nano particle with a bifluorescence mark, which is a mesoporous silicon dioxide nano particle formed by loading fluorescein isothiocyanate and rhodamine 6G containing a molecular lactam ring together. The invention also discloses a preparation method of the mesoporous silicon dioxide nano particle with the bifluorescence mark and application of the mesoporous silicon dioxide nano particle in detecting a pH value in cell lysosome. The mesoporous silicon dioxide nano particle containing an alkali sensitive FITC (Fluorescein Isothiocyanate) fluorescence molecule and an acid sensitive rhodamine-lactam molecule is used for detecting the pH of the lysosome by a ratio method and has a wide application prospect in the aspect of early diagnosis of diseases, such as cystic fibrosis, asthma, and the like.

The invention discloses a preparation method of a silver-azotetrazole metal organic framework material and an application of the silver-azotetrazole metal organic framework material in organic dye photodegradation. The preparation method comprises the following steps: by adopting a simple solvent diffusion method, reacting metal silver salt with ethylenediamine to prepare a stable metal solution, and reacting the solution with an organic solution of 5,5'-azotetrazole to prepare the silver-azotetrazole metal organic framework material with photodegradation efficiency. The material disclosed by the invention can be used for decomposing 86.3% of an organic dye namely rhodamine 6G within 60 minutes under the irradiation of ultraviolet light. The material is simple in preparation process and high in photocatalytic degradation efficiency of organic dye, so that the material is an organic pollutant photodegradation material with great potential.

The invention relates to a Raman spectrum high electromagnetic enhancement substrate coated with a gain medium, which is an enhancement Raman spectrum substrate structure on a surface coated with gain medium on a metal blind hole array surface, wherein round, rectangular or triangular blind hole arrays are uniformly distributed on substrate metal, and the coated gain medium is a rhodamine6G/polymethylmethacrylate film which is prepared by dissolving dye molecules in an organic solvent. The preparation comprises the following steps of: dissolving rhodamine6G and polymethylmethacrylate into dichloromethane; then coating the obtained solution on the surface of a substrate metal chromium film and drying to form a film coated with the gain medium on the surface of the substrate metal chromium film; and processing blind hole arrays on the surface of the film by adopting a focused ion beam lithograplay technology. The Raman spectrum high electromagnetic enhancement substrate has the advantages that the gain medium material is introduced onto a traditional SERS (Surface Enhanced Raman Scattering) substrate to compensate metal loss so as to obtain SERS enhancement factors far higher than that of a substrate without a gain medium substrate, so that the practicality of the SERS is further improved, and a technical reference is provided for the research of SERS enhancement mechanism.

The invention discloses a compound (I) P-N-methyl cyclopentaldehyde rhodamine 6G pH fluorescence molecular probe as well as a preparation method and use thereof. When hydrogen ions are present in the detected environment, under the action of hydrogen ions, the rhodamine 6G lactam spiral ring in the probe molecular compound (I) is opened in ring from closed state, and the color of the solution is obviously changed from achromatic color to orange red with strong fluorescence-emission. The compound I can be used for high-sensitivity naked eye color identification for hydrogen ions and also used as a fluorescent identifying probe.

The invention discloses a rhodamine 6G lead ion fluorescent probe. A compound with a structure shown in the formula I and lead ions undergo a reaction so that the solution color and fluorescence properties are changed. The fluorescent probe can be used for lead ion fluorescence detection and visual detection.

The invention discloses a compound (I), namely a rhodamine 6GpH fluorescent molecular probe containing a biphenyl group, as well as a preparation method and application thereof. According to the preparation method, rhodamine 6G and hydrazine hydrate react to prepare rhodamine 6G hydrazide, and a condensation reaction is carried out on the rhodamine 6G hydrazide and butyryl biphenyl to prepare the compound I. The compound has excellent optical property, fluorescence intensity is obviously enhanced when reacting with hydrogen ions, and the compound can be used for detecting the hydrogen ions in a solution.

The invention belongs to the field of random lasers and relates to a zinc oxide nanorod based random laser and a laser gain medium manufacturing method. The random laser comprises a pump laser, a reflecting mirror, a cylindrical lens and a zinc oxide nanorod based random laser gain medium, and the random laser gain medium comprises a zinc metal sheet, a micron zinc metal structure, zinc oxide nanorods, a polymethyl methacrylate film and rhodamine 6G. According to the method, the zinc oxide nanorods directly grow on the surface of the zinc metal sheet by means of hydrothermal oxidation to obtain the random laser gain medium on the basis of the zinc oxide nanorods. The zinc oxide nanorod manufacturing method is simple, low in cost and nontoxic; by adoption of the zinc oxide nanorods on the surface of the zinc metal sheet as scattering particles, random laser output is realized. The random laser is simple in structure and has advantages of low cost and environment friendliness.

The invention discloses an application of UiO-66 coated with rhodamine 6G in fluorescence detection of ferric ions. The UiO-66 coated with rhodamine 6G is a rate type fluorescent probe for fluorescence detection of ferric ions, wherein fluorescence of UiO-66 is taken as a reference signal, and fluorescence of rhodamine 6G is taken as a responding signal. Compared with existing fluorescence detection of ferric ions, the UiO-66 coated with rhodamine 6G taken as the fluorescent probe is easy to store, very good in stability and good in selectivity to Fe<3+>, and can recognize and detect the Fe<3+> specifically. The application is simple and convenient to operate and high in sensitivity, and also can avoid errors caused by concentration of the fluorescent probe and the environment effectively.