The invention is applicable to the technical field of Raman spectrum detection, and provides a surface enhanced Raman spectrum detection method based on an aggregation restabilization strategy, which comprises the following steps: S1, uniformly mixing colloidal gold with a to-be-detected object; s2, adding an aggregating agent into a system in the S1, and quickly and uniformly mixing, so that the colloidal gold is aggregated to generate a colloidal gold aggregate; s3, adding an acrylamide-methylene bisacrylamide solution into a system in S2, then adding a tetramethylethylenediamine solution and an ammonium persulfate solution, and uniformly mixing; s4, pouring the mixture obtained in S3 into a beaker, and placing the beaker in an incubator until the colloidal gold aggregate SERS detection substrate with stable polyacrylamide hydrogel is formed; and S5, taking out the SERS detection substrate formed in S4, and placing the SERS detection substrate on the surface of the tin foil paper.According to the detection method, the sensitivity of SERS detection is improved, and the stability and uniformity of SERS detection of the gold nanoparticle aggregate are greatly improved.