Surface enhanced Raman spectrum detection method based on aggregation restabilization strategy

A surface-enhanced Raman and spectroscopic detection technology, applied in Raman scattering, preparation of test samples, material excitation analysis, etc., can solve the problems of poor stability and low sensitivity of colloidal gold SERS detection, and achieve low cost, high-quality Mann enhancement effect, sensitivity enhancement effect

Pending Publication Date: 2022-07-29
OCEAN UNIV OF CHINA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a surface-enhanced Raman spectroscopy detection method based on an aggregation restabilization strategy, aiming to solve the problems of low detection sensitivity and poor stability of existing colloidal gold SERS

Method used

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  • Surface enhanced Raman spectrum detection method based on aggregation restabilization strategy
  • Surface enhanced Raman spectrum detection method based on aggregation restabilization strategy
  • Surface enhanced Raman spectrum detection method based on aggregation restabilization strategy

Examples

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Embodiment 1

[0042] Example 1: Detection of 4-MBA probe molecules by gold nanoparticles with different concentrations

[0043] The embodiment of the present invention provides a surface-enhanced Raman spectroscopy detection method based on an aggregation and restabilization strategy, comprising the following steps:

[0044] S1: Mix 1 mL of colloidal gold (gold nanoparticles) with a concentration of 0.05 to 0.6 nM with 20 μL of 10 -5 M's 4-MBA was mixed evenly with a vortex shaker and placed at room temperature for 15min;

[0045] S2: Add 60 μL of sodium chloride solution with a mass concentration of 1M to the system, mix it uniformly with a vortex meter, and place it for 10 s to promote the aggregation of gold nanoparticles, resulting in colloidal gold (gold nanoparticles) aggregates (AuNAs);

[0046] S3: Add 0.5 mL of acrylamide-methylenebisacrylamide solution with a mass concentration of 30% to the solution system of S2, wherein the mass ratio of acrylamide and methylenebisacrylamide is...

Embodiment 2

[0050] Example 2: Detection of 4-MBA probe molecules with different concentrations of acrylamide

[0051] The embodiment of the present invention provides a surface-enhanced Raman spectroscopy detection method based on an aggregation and restabilization strategy, comprising the following steps:

[0052] S1: Mix 1 mL of 0.2 nM colloidal gold (gold nanoparticles) with 20 μL of 10 -5 M's 4-MBA was mixed evenly with a vortex shaker and placed at room temperature for 20min;

[0053] S2: Add 60 μL of sodium chloride solution with a mass concentration of 1M to the system, mix quickly with a vortex meter, and place it for 15 s to promote the aggregation of gold nanoparticles, resulting in colloidal gold (gold nanoparticles) aggregates (AuNAs);

[0054] S3: Add 0.25-2.5 mL of acrylamide-methylenebisacrylamide solution with a mass concentration of 30% to the solution system of S2, wherein the mass ratio of acrylamide and methylenebisacrylamide is 29:1, use a pipette Pipette 2 μL of te...

Embodiment 3

[0058] Example 3: Stability of PAH-AuNAs for 4-MBA probe molecular detection

[0059] The embodiment of the present invention provides a surface-enhanced Raman spectroscopy detection method based on an aggregation and restabilization strategy, comprising the following steps:

[0060] S1: Mix 1 mL of 0.2 nM colloidal gold (gold nanoparticles) with 20 μL of 10 -5 M's 4-MBA was mixed evenly with a vortex shaker and placed at room temperature for 20min;

[0061] S2: Add 60 μL of sodium chloride solution with a mass concentration of 1M to the system, mix quickly with a vortex meter, and place it for 15 s to promote the aggregation of gold nanoparticles, resulting in colloidal gold (gold nanoparticles) aggregates (AuNAs);

[0062] S3: Add 0.5 mL of acrylamide-methylenebisacrylamide solution with a mass concentration of 30% to the solution system of S2, wherein the mass ratio of acrylamide and methylenebisacrylamide is 29:1, and pipette to remove 2 μL of tetramethylethylenediamine ...

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Abstract

The invention is applicable to the technical field of Raman spectrum detection, and provides a surface enhanced Raman spectrum detection method based on an aggregation restabilization strategy, which comprises the following steps: S1, uniformly mixing colloidal gold with a to-be-detected object; s2, adding an aggregating agent into a system in the S1, and quickly and uniformly mixing, so that the colloidal gold is aggregated to generate a colloidal gold aggregate; s3, adding an acrylamide-methylene bisacrylamide solution into a system in S2, then adding a tetramethylethylenediamine solution and an ammonium persulfate solution, and uniformly mixing; s4, pouring the mixture obtained in S3 into a beaker, and placing the beaker in an incubator until the colloidal gold aggregate SERS detection substrate with stable polyacrylamide hydrogel is formed; and S5, taking out the SERS detection substrate formed in S4, and placing the SERS detection substrate on the surface of the tin foil paper.According to the detection method, the sensitivity of SERS detection is improved, and the stability and uniformity of SERS detection of the gold nanoparticle aggregate are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of Raman spectrum detection, and in particular relates to a surface-enhanced Raman spectrum detection method based on an aggregation and restabilization strategy. Background technique [0002] Surface-enhanced Raman spectroscopy (SERS) is a molecular vibrational spectroscopy technique that utilizes the Raman "hot spots" generated by nanostructures by adsorbing or approaching a target on a nanostructured surface with surface plasmons. The Raman scattering signal of the object is greatly enhanced (the enhancement factor is usually greater than 10 5 ). SERS technology has the characteristics of high sensitivity, fast detection speed, little interference by moisture, molecular fingerprint, and the potential of non-destructive detection. In recent years, with the rapid development of spectroscopic instruments, various hand-held Raman spectrometers have appeared one after another. Because of their advantages of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/38
CPCG01N21/658G01N1/38
Inventor 王凯强林洪曹立民隋建新岳子琳
Owner OCEAN UNIV OF CHINA
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