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Raman multiple detection method based on silver nanoparticles tetrahedron

A technology of silver nanoparticles and multiple detection, applied in the field of analytical chemistry, can solve problems such as human health effects, allergic reactions, thyroid cancer, etc., and achieve the effects of Raman enhancement, reduced interference, and good specificity

Active Publication Date: 2015-05-06
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This kind of drug is easy to remain in the animal body and cause serious impact on human health, such as damage to the brain nervous system, cause hemolytic anemia, allergic reaction, cause thyroid cancer, etc.

Method used

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  • Raman multiple detection method based on silver nanoparticles tetrahedron
  • Raman multiple detection method based on silver nanoparticles tetrahedron
  • Raman multiple detection method based on silver nanoparticles tetrahedron

Examples

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Embodiment 1

[0038] (1) Synthesis of 10nm silver nanoparticles (AgNP)

[0039] Take a clean Erlenmeyer flask and place it in an ice bath, add 20mL of ultrapure water, 5mL of 1% polyvinylpyrrolidone and 0.6mL of 0.01mol / L sodium borohydride aqueous solution in sequence. Then 5 mL of polyvinylpyrrolidone with a mass fraction of 1% and 5 mL of an aqueous solution of silver nitrate with a mass fraction of 1% were simultaneously added to the conical flask at a rate of 30 mL / h, and stirred while adding, the solution changed from colorless to yellow. The resulting silver nanoparticles had a diameter of 10 nm.

[0040] (2) Silver nanoparticles modify DNA

[0041] Take 30μL of 20nM silver nanoparticles AgNP synthesized above in a PCR tube, add 1μL of 10μM DNA1 and mix well, then add 5μL of 5×tris-boric acid buffer solution and 1.25μL of 2mol / L NaCl solution to the system, and shake at room temperature for reaction After 12h, centrifuge at 13000r / min for 10min, remove the supernatant, add ultrapur...

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Abstract

The invention discloses a raman multiple detection method based on a silver nanoparticles tetrahedron, belonging to the technical field of analytical chemistry. The silver nanoparticles tetrahedron is successfully assembled by a DNA-mediated nanoparticles self-assembly technology; three segments of NDAs containing to-be-detected articles SDM, AFMI and OTA nucleic acid adapter segments and three beacon molecules are introduced into the silver nanoparticles tetrahedron by utilizing the special steric configuration of tetrahedron; a raman multiple-detection system based on the silver nanoparticles tetrahedron is successfully built; when the to-be-detected articles exist, the steric configuration of the tetrahedron changes to cause change of raman signals so as to detect; the change of raman signals of the three beacon molecules corresponds to the concentration of three targets, so that simultaneous detection of the three targets SDM, AFM1 and OTA is achieved; the method has extremely high sensitivity and good specificity; the effects on the detection result caused by random aggregation of the nanoparticles are avoided; and application of a raman sensing technology in practice is facilitated.

Description

technical field [0001] The invention relates to a method for multiple detection based on silver nanoparticle tetrahedron Raman, which belongs to the technical field of analytical chemistry. Background technique [0002] In 1928, Indian scientist Raman first discovered Raman scattering, that is, when a photon of known energy or wavelength and frequency interacts with a molecule, it causes molecular vibration and energy loss. Subsequently, Mr. Raman invented the first Raman spectrometer, for which he won the Nobel Prize in Physics. However, due to the relatively weak Raman signal, this technique has not been widely used. Later, with the invention of lasers, CCDs and optical filters, the performance of Raman spectrometers was greatly improved, which led to the widespread popularization and application of Raman spectrometers. Surface-enhanced Raman scattering (SERS) is based on the original Raman scattering, using the electromagnetic field enhancement effect on the surface of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCC12Q1/68G01N21/65
Inventor 徐丽广郝昌龙胥传来匡华严文静马伟刘丽强
Owner JIANGNAN UNIV
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