670 results about "CHEMISTRY METHODS" patented technology

A metallo-construct, which may be a peptide, is provided for use as a biological, therapeutic, diagnostic imaging, or radiotherapeutic agent, and for use in library or combinatorial chemistry methods. The construct has a conformationally constrained global secondary structure obtained upon complexing with a metal ion. The peptide constructs are of the general formula:where X is a plurality of amino acids and includes a complexing backbone for complexing metal ions, so that substantially all of the valences of the metal ion are satisfied upon complexation of the metal ion with X, resulting in a specific regional secondary structure forming a part of the global secondary structure; and where R1 and R2 each include from 0 to about 20 amino acids, the amino acids being selected so that upon complexing the metal ion with X at least a portion of either R1 or R2 or both have a structure forming the balance of the conformationally constrained global secondary structure. All or a portion of the global secondary structure, which may be sychnologic or rhegnylogic, may form a ligand or mimic a known biological-function domain. The construct has substantially higher affinity for its target upon labeling with a metal ion.

The invention discloses a Gaussian process regression-based method for predicting state of health (SOH) of lithium batteries, relates to a method for predicting the SOH of the lithium batteries, belongs to the fields of electrochemistry and analytic chemistry and aims at the problem that the traditional lithium batteries are bad in health condition prediction adaptability. The method provided by the invention is realized according to the following steps of: I. drawing a relation curve of the SOH of a lithium battery and a charge-discharge period; II, selecting a covariance function according to a degenerated curve with a regeneration phenomenon and a constraint condition; III, carrying out iteration according to a conjugate gradient method, then determining the optimal value of a hyper-parameter and bringing initial value thereof into prior distribution; IV, obtaining posterior distribution according to the prior part; V, obtaining the mean value and variance of predicted output f' without Gaussian white noise; and VI, together bringing the practically predicted SOH of the battery and the predicted SOH obtained in the step V into training data y to obtain the f', then determining the prediction confidence interval and predicting the SOH of the lithium battery. The method provided by the invention is used for detecting lithium batteries.

An integrated chromatography-immunoassay system for integrated chromatography-immunoassay system includes a chromatographic unit that receives labeled nano-structured probes comprising nano particles and antibodies attached to the nano particles, and a test membrane comprising coating antigens. The chromatographic unit allows the labeled nano-structured probes to diffuse there through and into the test membrane, wherein the antibodies on the nano particles are bound to the coating antigens. A laser device emits a laser light to illuminate the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane. A spectral analyzer obtains a Raman spectrum from light scattered from the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane, and to identify a spectral signature in the Raman spectrum associated with the antibody-antigen pair, which enables detection and identification of the antibody.

An integrated system for modeling, simulating and analyzing chemical and biochemical reactions includes a modeling environment for constructing a model of a chemical or biochemical reaction. The system also includes a simulation engine accepting as input said constructed model of the chemical or biochemical reaction and generating as output an expected result for a first chemical reaction using a first type of computational model and an expected result for a second chemical reaction using a second type of computational model. An analysis environment communicates with the simulation engine and displays the expected result

The invention provides a method for identifying a candidate imaging probe, the method comprising: a) contacting a first library of candidate compounds with a target biomacromolecule, b) identifying a first member from the first library exhibiting affinity for the first binding site; c) contacting the first member identified from the first library affinity for the first binding site with the target biomacromolecule; d) contacting a second library of candidate compounds with the first member and the target biomacromolecule, e) reacting the complementary first functional group with the second functional group via a biomacromolecule induced click chemistry reaction to form the candidate imaging probe; f) isolating and identifying the candidate imaging probe; g) preparing the candidate imaging probe by chemical synthesis; and h) for imaging applications, converting the candidate imaging probe into an imaging probe.

The invention relates to a method for simultaneously detecting three antibiotic residues including streptomycin, chlorampenicol and tetracycline based on nucleic acid aptamers and quantum dots and belongs to the technical field of analytical chemistry. Three-section complementary cDNA 1 sequences are designed and synthesized and are complemented with three antibiotic aptamers DNA sequences and are further complemented with respective capturing probe DNA sequences of three antibiotics and complementary cDNA 2 sequence part. The three cDNAs 1 are first hybridized with aptamer DNAs supplemented with each other to form double-stranded DNAs. When target antibiotics exist in a sample, the aptamer DNAs are combined with corresponding antibiotics as a priority to cause the double-stranded DNAs to be de-hybridized and release cDNAs 1. The free cDNAs 1 are combined with capturing probe DNAs modified on the surface of a gold electrode, and cDNAs 2 modified with three quantum dots are hybridized with the other end of each cDNA 1. The three quantum dots on the surface of the gold electrode generate dissolution volt-ampere peaks corresponding to the target antibiotics. The three antibiotics are detected by utilizing the relations of changes of peak current values and antibiotic concentration. The method is good in repeatability and stability and high in sensitivity and can be used for directly measuring the three antibiotic residues in samples such as milk.

An integrated system for modeling, simulating and analyzing chemical and biochemical reactions includes a modeling environment for constructing a model of a chemical or biochemical reaction. The system also includes a simulation engine accepting as input said constructed model of the chemical or biochemical reaction and generating as output an expected result. An analysis environment communicates with the simulation engine and displays the expected result.

The invention relates to a method for rapidly detecting the residuals of organophosphorus pesticides in vegetables by utilizing an Au nano-particle colorimetric method, which belongs to the technical field of analytical chemistry. The method provided by the invention comprises the following detection steps: preparation of Au nano-particles (AuNps); establishment of a method for detecting organophosphorus pesticides, actual sample detection and the like. The method provided by the invention can simply, rapidly and sensitively detect methamidophos in the organophosphorus pesticides according to the color change of an Au nano-particle system, has high sensitivity and provides convenience for further research, production, supervision and the like.

An integrated chromatography-immunoassay system for integrated chromatography-immunoassay system includes a chromatographic unit that receives labeled nano-structured probes comprising nano particles and antibodies attached to the nano particles, and a test membrane comprising coating antigens. The chromatographic unit allows the labeled nano-structured probes to diffuse there through and into the test membrane, wherein the antibodies on the nano particles are bound to the coating antigens. A laser device emits a laser light to illuminate the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane. A spectral analyzer obtains a Raman spectrum from light scattered from the labeled nano-structured probes having the antibodies bound to the coating antigens on the test membrane, and to identify a spectral signature in the Raman spectrum associated with the antibody-antigen pair, which enables detection and identification of the antibody.

The invention discloses a detection method of a kanamycin residue based on a nucleic acid aptamer, and belongs to the technical field of analytic chemistry. The method comprises the steps that by using a sulfydryl covalent modification mode, ssDNA (single-stranded Deoxyribonucleic Acid) complementary with the two ends of a kanamycin aptamer is modified on the surfaces of AuNPs (Gold Nanoparticles) respectively; two kinds of functionalized AuNPs are obtained; the kanamycin aptamer is added and pairwise bound with ssDNA on the surfaces of the functionalized AuNPs; spatial distances among the AuNPs are closed; the AuNPs are mediated to be aggregated; after the AuNPs are mixed with kanamycin samples with different concentrations, specific binding between kanamycin and the aptamer allows an aggregation to be disaggregated, which is manifested by a change of an AuNPs characteristic absorption peak in an ultraviolet-visible spectrum; and the kanamycin residue is detected by using a relation between a change of the absorbency at a 527-nanometer part and the concentration of the kanamycin. The method is good in repeatability and stability, easy to operate and high in sensitivity, and can be used for directly determining the kanamycin residues in samples such as pretreated milk.

The invention relates to an ordinary brass full-elemental analysis apparatus based on a laser-induced breakdown spectroscopy, and a method thereof, and belongs to the technical field of analytical chemistry. The apparatus comprises a laser, a spectrometer, a delay generator, a focusing lens, a collecting lens, optical fiber, a sample and a computer. The laser and the spectrometer are respectivelyconnected to the delay generator. The spectrometer is connected with the computer. The optical fiber is connected with the spectrometer. The focusing lens is arranged between the laser and the sample. The collecting lens is connected with the spectrometer by the optical fiber. The analysis method comprises: establishing working curves of Cu, Pb, Fe and Ni, and verifying the working curves. The apparatus and the method are applicable for analyzing the Cu content, the Pb content, the Fe content and the Ni content in the ordinary brass.

The invention provides a method for determining 101 pesticide residues in medlar, and belongs to the technical field of analytic chemistry. The method comprises the steps of 1 pretreating a sample, wherein an acetonitrile extracting solution is added into the smashed medlar sample, and then extraction salt is added for performing extraction; S2, performing purification, wherein a Sin-QuEChERS Nanopurification pipe is adopted for performing purification, and after a supernate is filtered, LC-MS/MS analysis is performed. Experiments prove that by adopting the method, the pesticide separation effect is good, on the basis of labelling recycling rate and precision experiment calculation, the measured result is accurate and reliable, the detection limit, the quantitation limit and various otherindexes of the method meet the requirement for multiple pesticide residue analysis, the method can be applied to qualitative and quantitative detection for various pesticide residues in medlar, the import and export trade for medlar in China can be promoted, and that great significance in ensuring human body health is achieved.

The invention belongs to the field of analytical chemistry and relates to the measurement for the transferring amount of phthalate and ethylhexyl adipate in fatty food contact materials, in particular to the use of an adsorbent chromatography colume for purifying and separating the phthalate and ethylhexyl adipate in fatty food simulant liquid, and the use of a combined gas chromatography mass spectrometry for measuring the content thereof. The pre-treatment method which is provided by the invention for the purification and separation of the adsorbent chromatography colume has the advantages of rapidity, strong selection property, small pollution, and low detection limit. The invention can achieve the measurement for the transferring amount of phthalate and ethylhexyl adipate in fatty food contact materials.

Provided is a biological enzyme deinking method for waste paper, in which flotation applies polyoxyethylene alkyl ether as flotation auxiliary, deinking process applies compound enzyme as deinking agent. The method improves the white content of the regenerated pulp by chemistry method, which is enhanced to 5-8% higher, and the COD value of the discharged sewage reduced more than 60%. The paper pulp filtration performance is further increased and the strength is similar, thus it can meet requirement of making different papers.

The invention relates to a combined gas chromatography mass spectrometry measuring method for measuring the pesticide residue in leather, which belongs to the field of analytical chemistry. The method adopts normal hexane and acetonitrile to perform ultrasonic extraction to a leather sample and separates and purifies the sample extracting solution by various means such as liquid-liquid extraction, gel permeation chromatograph, adsorbent chromatography column, solidoid extraction column, and the like by aiming to the characteristics that the leather sample has high oil content, the residual processing assistant is complex, and the like, and the pesticide residue of the processed sample extracting solution is measured by the combined gas chromatography mass spectrometry. The invention has the advantages of high selectivity, wide range of application and low detectability, realizes the measurement of the pesticide residue in the leather, creates a new thought for the measurement of the pesticide residue in the leather and fills in a gap for the measurement of the pesticide residue.

The invention discloses a method for measuring trace chloridion and sulfate radical in loprazolam samples by ion chromatography and belongs to the field of analytic chemistry. The method includes the followings steps of preparing and analyzing Cl- and SO42- mixed standard solution and obtaining a linear equation of concentration and peak area, pretreating the loprazolam samples, feeding the treated loprazolam samples to an ion chromatography by a disposable needle type filter, utilizing 3.6mmol/L sodium carbonate as leacheate and analyzing and measuring the content of the chlorodion and the sulfate radical according to the obtained linear equation, wherein a chromatographic column adopted in the ion chromatography is a high-capacity chromatographic column, the inner diameter of filler of the chromatographic column is 5 micrometers, the temperature of a column temperature box is set to be 45 DEG C, sampling input quantity is 100 microliters, applied curb current is 40mA, and flow velocity is 0.8ml/min. The method is quick and simple, high in accuracy, convenient and easy to operate and applicable to quality control in the process of production of loprazolam, the integral analyzing process can be completed within 25min, and the detection requirements of the fine chemical industry are met.

A partial air cooling method for a precast aperture passage in an investment casting shell comprises the following steps: 1, forming a shell on a wax pattern surface according to the regular investment casting process; 2, sticking a material that can be removed from the inside of the shell through heating or with a chemistry method onto a part, needing quick cooling, of the shell surface; 3, continuing the shell forming, burying the material that can be removed from the inside of the shell through the heating or with a chemistry method into the shell, and enabling a part of the material at an air inlet and an air outlet to be naked; 4, removing the material in the shell and forming a cooling air passage; 5, performing high temperature roasting to the shell with the interior material removed according to the process requirements; and 6, using the shell for casting, introducing air into the cooling air passage according to the partial cooling requirement, and forming a strong partial convection heat exchange. According to the shape and the size of the required partial cooling part, the removable material is flexibly arranged to form a specific air passage structure, the cooling range controllability is high and the cooling rate can be controlled through the intensity of airflow.

The invention discloses a method for quickly testing melamine content in milk powder by using a gold nanoparticle colorimetric method, which belongs to the technical field of analytical chemistry. The invention comprises the steps of preparing gold nanoparticles (AuNPs), derivatizing crown ether, conjugating gold nanoparticle and crown ether, self assembling by applying the gold nanoparticles, detecting melamine by molecular recognition, detecting the practical sampling and the like. The invention prepares a gold nanoparticle self-assembly material, can simply, quickly and flexibly detect the melamine content in the milk powder according to the color change of a gold nanoparticle system, simultaneously improves the detection sensitivity greatly, achieves the purpose of high flux, and provides convenience for the subsequent research, production, supervision and the like.

The invention discloses a broad spectrum insect situation automatic forecasting method based on the computer vision technology. According to the method, insect images of captured target insects are obtained at regular time; an obtained current insect image and a yesterday insect image are subjected to filtering treatment so as to obtain a current insect profile diagram and a yesterday insect profile diagram; the current insect profile diagram and the yesterday insect profile diagram are subjected to subtraction treatment so as to obtain an increment diagram; an insect characteristic spectrum is established and updated as per the increment diagram; the increment count value of insect quantity of this time is computed as per the increment diagram; the area count value of insect quantity is computed as per the insect characteristic spectrum; and further, the weighted average of the increment count value of insect quantity of this time and the area count value of insect quantity is obtained, and then the total amount of insects can be obtained through calculation. The broad spectrum insect situation automatic forecasting method combines machine vision, pattern recognition and biological chemistry methods, automatically forecasts the injurious insects as needed, obtains the population density of the injurious insects in the field, and provides scientific decision bases for early warning and comprehensive control of insect damage.