4 results about "Molecular vector" patented technology

Provided are a phytase mutant and a coding DNA molecule thereof, a vector, and a host cell. The phytase mutant comprises an amino acid sequence having at least 90% identity with SEQ ID NO: 3, and compared with SEQ ID NO: 3, and contains an amino acid substitution at at least one position selected from the group consisting of 36, 126, 211, 253, 258, and 266. The heat resistance of the mutant is significantly improved, thus facilitating the wide application of phytase in feed.

The application discloses a plant-expressed varicella-zoster virus gB recombinant antigen and a preparation method, product and application thereof, and belongs to the technical field of plant molecular biology and vaccine engineering. The technical problems to be solved are that the existing VZV gB protein has low heterologous expression, poor stability and insufficient antigen activity; the existing VZV vaccine has high production cost, is difficult to popularize and has a single antigen with an immune escape risk; and the plant expression system is not mature in VZV gB antigen production, and lacks a complete preparation scheme and related products. The technical solution is that the natural VZV gB protein is modified, a furin protease cleavage site is knocked out, and a H527P mutation is introduced, related nucleic acid molecules, vectors and plant source cells are constructed, high-activity antigens are obtained through culture and purification, and the high-activity antigens are applied to the preparation of VZV drugs / vaccines, and have the advantages of low cost, high safety and strong immunogenicity.

This application provides a virtual drug screening method, a target computing device, and a storage medium, relating to the field of drug screening technology. The method includes: dividing all small drug molecules into multiple groups of small molecules based on the total number of target accelerators included in multiple computing nodes; encoding a group of small molecules corresponding to each computing node within each group of small molecules to obtain a molecular vector; encoding protein pockets in the target target to obtain a pocket vector; determining multiple candidate small molecules similar to the target target from the drug molecules included in the target drug molecule library based on the pocket vectors and molecular vectors; and using a physical simulation method to determine highly active small molecules that stably bind to the target target from the multiple candidate small molecules. In this application, multiple groups of small molecules with consistent data amounts are allocated to multiple target accelerators to avoid wasting computing resources due to idle target accelerators, thereby improving the screening efficiency of small molecules.

The application belongs to the technical field of biological medicine, and particularly relates to a Clostridium perfringens delta toxin specific binding protein, a nucleic acid molecule, a carrier, a cell and a preparation method and application thereof. The application provides a Clostridium perfringens delta toxin specific binding protein containing CDRs of a specific sequence, the Clostridium perfringens delta toxin specific binding protein can effectively and cytotoxicity of the delta toxin, thereby protecting the tissue from damage, and has higher specificity, which provides a key scheme for solving the problem of lack of high-efficiency prevention and control diagnosis means for Clostridium perfringens delta toxin induced infectious diseases, and has important clinical and application values.