54 results about "Molecular vector" patented technology

The present invention relates to peptides that bind with high specificity and which functionally interact with the transferrin receptor (“TfR”) and which may be used in making molecular vehicles that carry biomolecules across membranes, including, e.g., across the blood brain barrier or the gastrointestinal tract. TfR specific binding moieties may also be used alone or as components in specific molecules that target the transferrin / transferrin receptor transport system. The invention relates more specifically to VNAR single chain antibodies derived from nurse shark that bind to TfR, compounds and compositions comprising a TfR specific VNAR binding moiety, methods for preparing them, diagnostic and therapeutic methods of use in vitro or in vivo, e.g., to diagnose, treat and / or prevent a pathological condition, disorder or disease in which it is beneficial to deliver a heterologous biomolecule across the blood brain barrier by association with a TfR specific VNAR binding moiety. Other uses for TfR specific VNAR binding moieties of the invention include, e.g., regulating the interaction of iron-charged transferrin with TfR (receptor cycling or cell surface presentation), such as may be therapeutic in treatment of certain cancer cells and tumors of various tissue types.

The invention belongs to the technical field of biotechnology, and relates to a tumor-targeted diagnosis nuclear magnetic resonance contrast agent and a preparation method thereof. The tumor-target diagnosis nuclear magnetic resonance contrast agent is prepared from a high molecular material, polyethylene glycol, a polypeptide, a dual-function ligand and gadolinium chloride by taking the polypeptide as a target head group, taking the tree-like high polymer material as a basic high molecular vector and connecting a small molecular contrast agent to the surface. In the invention, a polypeptide-modified high molecular material screened by using a phage display technology enters cells in an endocytic way, so that the introjection of the contrast agent by tumor cells is increased, and the characteristic of high safety is achieved. The target head group polypeptide used in the invention has the advantages of transferrin, can be used for effectively avoiding the interference of endogenous transferrin, has high targeting and diagnosing efficiencies, is simple to prepare, and can be further applied to target diagnosis of other tumor tissues.

The invention belongs to the technical field of recombinant fusion protein medicaments. In the method, capsid protein genes cotC of spores of bacillus subtilis are used as molecular vectors and are recombined with human serum albumin hsa genes, so that integrated recombinant plasmid for fusion expression is constructed; after the bacillus subtilis is transformed through the recombinant plasmid for fusion expression of the human serum albumin HAS, exogenous genes are integrated in amylase genes amyE at specific sites on chromosomes through homologous recombination, and the amylase genes are subjected to insertional inactivation, so that a recombinant strain has no amylase activity, the recombinant bacillus subtilis strain comprising fusion expression CotC-HAS is successfully screened, and the human serum albumin is displayed on the surface of the spore produced by inducing the recombinant strain. The recombinant spore can be directly and orally taken by animals; and the recombinant spore has a part of medicinal effects and nutrition functions of the human serum albumin on the basis of keeping physiological property of the spore.

Provided are a method and a kit for accurately and rapidly detecting ten types of targeting pneumonia bacteria: Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Klebsiella pneumoniae, Pseudomonas aeruginosa, Moraxella catarrhalis, methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus. A set of primer pairs directed to their respective target regions contained in the DnaJ gene, etc., of the ten types of pneumonia causative bacteria is designed for the ten bacterial strains and used to amplify gene products. A set of bacterial strain-specific probe pairs is further designed for the ten bacterial strains such that the probe pairs hybridize with the amplification products via sequences in the respective target regions differing from the sequences hybridized by the set of primer pairs. A first probe-bound labeled high molecular carrier in which plural types of first probes for the pneumonia bacteria are bound to a labeled high molecular carrier and a solid-phase second probe-carrying developing support are used as the set of probe pairs to perform nucleic acid chromatography.

The invention discloses preparation and an enzyme-linked immunosorbent assay method for heavy metal mercury polyclonal antibody in the technical field of heavy metal detection. The polyclonal antibody is prepared for the enzyme-linked immunosorbent assay by the following steps of: bonding the heavy metal mercury at one end of penicillin G sodium serving as a dual-functional chelating agent; coupling the other end with high molecular carrier proteins, namely bovine serum albumin and ovalbumin, to form immunogen MMPA-BSA and envelope antigen MMPA-OVA of mercury-chelating agent-protein; and finally emulsifying and immunizing the immunogen MMPA-BSA to prepare the polyclonal antibody. The prepared antibody has high specificity for the mercury ions, a cross reaction ratio with all other metals of less than 0.001 percent apart from the cross reaction ratio with cadmium of 7.9 percent and an adding recovery ratio of between 91.4 and 120 percent; and thus the antibody can be used for the assayof the heavy metal mercury in a water sample and can also be developed into rapid immunological detection technology for rapidly detecting the heavy metal mercury in other samples such as agricultural products and the like by combining certain pre-processing technology.

The invention relates to a molecular vector for single molecule detection. The molecular vector comprises a substrate and a metal layer, wherein one surface of the substrate is provided with a plurality of three-dimensional nanostructures; the three-dimensional nanostructures are respectively arranged along a first direction and a second direction at intervals; and the metal layer is coated on the surface of the three-dimensional nanostructures and on the surface of the substrate between adjacent three-dimensional nanostructures.

In the invention which belongs to the technical field of recombinant fusion proteins, silkworm alcohol dehydrogenases as exogenous proteins are displayed on surfaces of bacillus subtilis. Capsid protein genes cotC of spores of the bacillus subtilis, serving as molecular vectors, are recombined with silkworm alcohol dehydrogenase genes Bmadh to produce integrated recombinant plasmids which express the silkworm alcohol dehydrogenases BmADH in a fused manner so as to convert the bacillus subtilis; and exogenous genes are integrated in amylase genes amyE at special positions on a chromosome through homologous recombination, and the amylase genes are damaged, so that recombinant strains are free of the activities of the amylase genes; and therefore, recombinant bacillus subtilis strains containing fusion expressions CotC-BmaDH are successfully screened. Spores with fused proteins of silkworm alcohol dehydrogenases displayed on the surfaces are obtained through the inducible expression of the recombinant strains. In the invention, original alcohol dehydrogenases with instable temperature and pH become the fused proteins of the alcohol dehydrogenases with relatively stable activities under different environmental environments, and produced recombinant spores can be used for the direct oral administration of animals.

The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker protein and a protein with antimicrobial activity; for example, with antibacterial activity or antibacterial hydrolytic enzyme. The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria.

An assembled compound array chip is composed of supporter, several carriers assembled on said supporter, and polymer moleculae (oligonucleotide, polypeptide, polyose and peptide nucleic acid) synthesized at known positions on relative carrier. After the polymer moleculae act on particular fluid, the change of its natural can be detected optically.

The invention relates to a biological cascade reaction type photodynamic integrated biopolymer, a preparation method and applications thereof, and belongs to the field of medicine preparation. According to the biological cascade reaction type photodynamic integrated biopolymer (PEG4000-CDM-ZnPc-S-S-HA), a photosensitizer zinc phthalocyanine (ZnPc) and PEG 4000 are combined through a pH-acid responsive maleic acid amide linker; and the combined material is immobilized on a hyaluronic acid (HA) chain by using a disulfide linker capable of being cracked by oxidation reduction, wherein the PEG segment can enhance the blood circulation of a molecular carrier after intravenous administration to fall off after reaching an acidic tumor microenvironment, so that the residual ZnPc-S-S-HA is self-assembled into a large cluster in situ so as to avoid reverse diffusion and improve the tumor retention rate, and due to hydrophilicity, degradability and the capacity of specifically combining with CD44overexpressed on a tumor cell membrane, HA is used as a carrier substrate and a tumor targeting ligand.

The invention discloses an FFT efficient parallel achieving optimizing method based on a Loongson number three processor. The FFT efficient parallel achieving optimizing method is characterized by comprising the following steps which are carried out by means of base-2 butterfly computation. Firstly, initial parameters are set; secondly, the number of grades of FFT conversion is obtained; thirdly, all twiddle factors are obtained; fourthly, molecular vectors are divided and whether blocking processing is carried out or not is judged; fifthly, blocking processing is carried out. The problem that the speed-up ratio of an existing parallel FFT algorithm on the Loongson number three processor is low can be solved by means of the FFT efficient parallel achieving optimizing method based on the Loongson number three processor and efficient parallel of FFT on the Loongson number three processor can be achieved.

The invention discloses a gene GmCIB1 and a gene GmCRY2 of soybeans and blooming and senescence control applications of the genes. Specifically, the invention relates to the gene GmCIB1 and the gene GmCRY2 of soybeans, DNA molecules containing the genes, vectors, gene encoded protein and relevant transformed cells and transgenic plants, and further relates to applications of the genes in control of plant leaf senescence and blooming as well as a method for cultivating relevant transgenic plants, and the gene GmCIB1 and the gene GmCRY2 are represented as SEQ ID NO: 1 or 3.

The invention belongs to the technical field of biotechnology, and relates to a tumor-targeted diagnosis nuclear magnetic resonance contrast agent and a preparation method thereof. The tumor-target diagnosis nuclear magnetic resonance contrast agent is prepared from a high molecular material, polyethylene glycol, a polypeptide, a dual-function ligand and gadolinium chloride by taking the polypeptide as a target head group, taking the tree-like high polymer material as a basic high molecular vector and connecting a small molecular contrast agent to the surface. In the invention, a polypeptide-modified high molecular material screened by using a phage display technology enters cells in an endocytic way, so that the introjection of the contrast agent by tumor cells is increased, and the characteristic of high safety is achieved. The target head group polypeptide used in the invention has the advantages of transferrin, can be used for effectively avoiding the interference of endogenous transferrin, has high targeting and diagnosing efficiencies, is simple to prepare, and can be further applied to target diagnosis of other tumor tissues.

The invention relates to a preparation method for oral recombinant nutritive polypeptide supplementing human body essential amino acids, and belongs to the technical field of recombination fusion protein medicines. The preparation method comprises integrating a nutritive polypeptide gene np bringing with an enterokinase label into a pJS700 vector, then using a spore capsid protein gene cotC as a molecular vector and constructing fusion expression integrated type recombinant plasmid, then performing conversion on bacillus subtilis by using the fusion expression integrated type recombinant plasmid, so as to enable the exogenous gene to be integrated into amylase gene in chromosome, and screening out a recombinant bacillus subtilis strain containing fusion expression cotC-NP, and displaying the nutritive polypeptide on the spore surface by inducing the recombinant bacterial strain. The constructed recombinant spore with the enterokinase-labeled nutritive polypeptide displayed on the surface can be directly orally taken by animals or people and be freely digested and absorbed in intestinal tracts, becomes a novel oral sport nutrition health product, and possesses wide application value. The limit that BCAA comes from chemical synthesis extraction is overcome, and also the disadvantage that separation purification is complex when the nutritive polypeptide is produced through recombination fermentation is overcome.

The invention relates to the technical field of biology, and in particular relates to a phytase mutant, a DNA molecule for encoding the phytase mutant, a vector and a host cell. The optimal pH value of the phytase mutant is 5.5, under neutral pH, the tolerance is significantly improved, under the pH of 6.0, the relative enzyme activity of the mutant reaches 72.80%-79.25%, under the pH of 6.5, therelative enzyme activity of the mutant can still reach 32.99%-40.95%, so that unexpected technical effect is achieved, and wide applications of phytase in aquatic feed is facilitated.

The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker protein and a protein with antimicrobial activity; for example, with antibacterial activity or antibacterial hydrolytic enzyme. The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria.

The invention relates to the field of biotechnology, in particular to a phytase mutant, a preparation method and application thereof, a DNA molecule for encoding the phytase mutant, a carrier and a host cell. The mutant provided by the invention comprises a substitution of an amino acid at at least one position selected from the group consisting of 36, 126, 211, 253, 258, 266. The heat resistance of the mutant is remarkably improved, so that the phytase can be widely applied to feeds.

The invention relates to the field of biotechnology, in particular to a phytase mutant, a preparation method and application thereof, a DNA molecule for encoding the phytase mutant, a carrier and a host cell. The mutant provided by the invention comprises a substitution of an amino acid at at least one position selected from the group consisting of 67, 72, 79, 115, 121, 295, 300, 307, 318, 329, 360, 376 and 385. The heat resistance of the mutant is remarkably improved, so that the phytase can be widely applied to feeds.

The invention relates to a karaya gum complex polysaccharide microsphere used as a bioactive molecular vector and a preparation method thereof. The prior microsphere has poor mechanical property, poor stability in acid or alkali solution, complicated production processes and high cost. The karaya gum complex polysaccharide microsphere uses a compound of biological polysaccharide karaya gum (deacetylation) and chitosan as a raw material and glutaric dialdehyde as a cross-linking agent to carry out polymerization; the karaya gum complex polysaccharide microsphere with -CHO and -COOH surface functional groups is prepared, the surface of the microsphere is in a poriferous honeycomb structure, and the microsphere has the grain diameter from 0.6*10 microns to 2.1*10 microns, the mechanical strength from 4.6N to 8.12N / grain and the swelling coefficient from 0.67ml / g to 0.93ml / g and is stable in the environment with the pH value from 1 to 14. The karaya gum complex polysaccharide microsphere has simple production technology and good biocompatibility, does not have toxic side effects and can be used as a good vector of enzyme or other bioactive molecules.

The present invention relates to peptides that bind with high specificity and which functionally interact with the transferrin receptor (“TfR”) and which may be used in making molecular vehicles that carry biomolecules across membranes, including, e.g., across the blood brain barrier or the gastrointestinal tract. TfR specific binding moieties may also be used alone or as components in specific molecules that target the transferrin / transferrin receptor transport system. The invention relates more specifically to VNAR single chain antibodies derived from nurse shark that bind to TfR, compounds and compositions comprising a TfR specific VNAR binding moiety, methods for preparing them, diagnostic and therapeutic methods of use in vitro or in vivo, e.g., to diagnose, treat and / or prevent a pathological condition, disorder or disease in which it is beneficial to deliver a heterologous biomolecule across the blood brain barrier by association with a TfR specific VNAR binding moiety. Other uses for TfR specific VNAR binding moieties of the invention include, e.g., regulating the interaction of iron-charged transferrin with TfR (receptor cycling or cell surface presentation), such as may be therapeutic in treatment of certain cancer cells and tumors of various tissue types.

The invention relates to a method for preparing a recombining spore of surface display foreign protein, in particular to a recombining spore for surface display prawn white spot syndrome virus Vp28 protein of a bacillus subtilis spore. In the method, a bacillus subtilis spore capsid protein gene is used as a molecule vector and is recombined with an antigen protein gene on the surface of the prawn white spot syndrome virus to construct an integral recombining plasmid in fusion expression; the bacillus subtilis is converted to a bacillus subtilis recombining strain; and the recombining spore of the spore surface display prawn white spot syndrome virus protein produced by the recombining strain is induced.

The present invention relates to structured molecular vectors of formula (I), compounds of formula (II) and pharmaceutical compositions comprising such compounds. The invention also relates to such pharmaceutical compositions for use for preventing and / or treating a disease chosen among an inflammatory disease or a disease associated with a cognitive disorder. The invention further relates to such pharmaceutical compositions for use for preventing cognitive decline or restoring cognitive functions altered in brain injuries and / or in traumatic brain injuries and / or in a neuroinflammatory disease, and / or in a neurodegenerative disease.

The invention relates to a method for preparing a molecular carrier for molecular detection, which includes the following steps: setting an intermediate layer on a substrate; providing a carbon nanotube composite structure with a plurality of micropores, the carbon nanotube composite structure comprising a carbon nanotube structure and a prefabricated layer covering the surface of the carbon nanotube structure; disposing the carbon nanotube composite structure on the surface of the intermediate layer so that the surface of the intermediate layer is partially exposed; The carbon nanotube composite structure is dry etching the middle layer with a mask to obtain a middle layer with patterned protrusions; and depositing a metal layer on the surface of the patterned protrusions.

The disclosure relates to a carrier for use in single molecule detection. The carrier includes a flexible substrate and a metal layer on the flexible substrate. The flexible substrate includes a base and a bulge pattern located on a surface of the base. The bulge pattern includes a number of strip-shaped bulges intersecting with each other to form a net and define a number of recesses. The metal layer is located on the bulge pattern. The carrier for use in single molecule detection has a relative higher SERS and can enhance the Raman scattering.

The present invention relates to a recombinant spore preparation method in which the bacillus subtilis spore surface displays the lipase with catalytic activity. The method includes: taking the bacillus subtilis spore capsid protein gene as molecular carrier to recombine with lipase gene having triglyceride hydrolysis and transesterification function, constructing amalgamation expressed integrated recombinant plasmid, and transforming the bacillus subtilis to obtain the recombinant strain, induce the spore surface produced by the recombinant strain to display the recombinant spore of the lipase. Compared with the known method in this field, the present inventive method displays lipases with triglyceride hydrolysis function from different sources on the surface of Bacillus subtilis spores,uses the unique resistance of the spores to increase the catalytic activity and stability of lipases.

The invention belongs to the field of pharmacy, and relates to an o-phthalic dihydroxy polymer carrier capable of reversibly combining with a drug, a drug-polymer complex formed by the polymer carrier and a drug, and a drug-delivery drug constructed by the drug-polymer complex system. The o-phthalic dihydroxy polymer carrier prepared by the present invention can be reversibly combined with drugs to form a drug-polymer complex and a nanoparticle drug delivery system, which has the significant advantage of high drug loading, can reduce drug toxicity, improve drug stability, The purpose of improving the in vivo targeting of drugs and enhancing drug efficacy, the experimental results show that: the drug-polymer complex and its nanoparticle drug delivery system has the characteristics of pH-responsive drug release, which can significantly reduce drug toxicity and improve drug efficiency. The stability of the drug can exert passive targeting in vivo or active targeting after further modification of the target molecule, which can significantly improve the efficacy of the drug. It has a good clinical application prospect.

The invention belongs to the field of pharmacy, and relates to a multi-purpose targeting molecule, and applications of a compound and a drug delivery system modified by the multi-purpose targeting molecule in brain tumor diagnosis and treatment. According to the invention, the multi-purpose targeting molecule RGD-pHA being compatible to brain capillary endothelial cells and crossing the blood-brain barrier, being highly compatible to integrin and crossing the blood-brain tumor barrier, and targeting to the brain tumor cells is prepared by adopting a molecule splicing method; the invention further relates to preparation of fluorescein and a medicine modified by RGD-pHA, and a macromolecular carrier material, and applications of RGD-pHA in construction of the drug delivery system. The experimental result shows that the RGD-pHA can be specifically taken by brain capillary endothelial cells expressing the dopamine receptor for mediating the model drug or the nanometer drug delivery system to enter the brain, so that the model drug or the nanometer drug delivery system is specifically taken by the positive cells and the tumor sphere tissue expressing the integrin, the good brain tumor targeting effect is shown in vitro and vivo, and the efficiency in resisting brain tumor is obviously improved.