72 results about "Raman scattering spectra" patented technology

A surface-enhanced Raman scattering surface is prepared by anodizing an aluminum or aluminum alloy substrate with reversed polarity current to form pores of anodized oxide on the substrate, and electrodepositing silver or copper into the pores of the anodic oxide film to form needle-shaped metal particles in the pores of the anodic oxide film.

Described are systems and methods for variable angle Raman spectroscopy, in which electromagnetic radiation will be caused to intersect the sample under investigation at a plurality of angles of incidence, so as to provide Raman scattering spectra at each angle. One example use of measuring such spectra at multiple angles of incidence is to enable evaluation at a plurality of depths within the sample. In many implementations, the range of the angles of incidence will include, and extend to either side, of the critical angle.

The invention discloses a precious-metal micro-nanometer structure and a preparing method and application thereof. The preparing method includes the steps that a thermotropic shape-memory high-polymer material is dissolved to be coated and dried to obtain a shape-memory high-polymer film; at the specific temperature, the shape-memory high-polymer film is stretched 10% to 30%, the stretched shape-memory high-polymer film is naturally cooled to the room temperature, the shape-memory high-polymer film is kept the stretched state, a precious-metal nanometer particle layer is prepared on the surface of the stretched shape-memory high-polymer film, the product is put into the environment with the specific temperature, the stretched shape-memory high-polymer film is recovered to the original size in the stretched direction accordingly, and the precious-metal micro-nanometer structure is obtained. According to the preparing method, the precious-metal micro-nanometer structure with the special three-dimensional structure is prepared through the characteristic of the shape-memory high-polymer material, the prepared precious-metal micro-nanometer structure is used as a surface-enhanced-raman-scattering-spectroscopy active substrate, and the detection sensitivity can be remarkably improved.

A method of fabricating polypyrrole-coated silver particles having a core-shell structure via in-situ oxidative polymerization using silver nanoparticles and pyrrole monomers in the presence of an oxidizing agent. A surface enhanced Raman scattering (SERS) active substrate comprising the polypyrrole-coated silver particles is also disclosed. The surface enhanced Raman scattering (SERS) active substrate is demonstrated to facilitate the detection and measurement of surface enhanced Raman scattering (SERS) spectra of analytes with increased Raman signal intensity.

Disclosed are a pH sensor for use in a highly alkaline region of pH >11 comprising citrate-reduced gold nanoparticles and a method for calibrating pH of a solution in highly alkaline regions, based on variation in surface-enhanced Raman scattering spectra (SERS).

The invention relates to a method and a system for noninvasively detecting a blood alcohol content based on Raman scattering. The method comprises the following steps: acquiring Raman scattering spectrum information of one group of alcohol solution blood samples with known concentration by adopting a transmission and reflection manner, establishing a quantitative mathematic relation between a blood alcohol concentration and the Raman peak strength of a Raman scattering spectrum to form a calibrating model; training the calibrating model and obtaining an optimal calibrating model; acquiring Raman scattering spectrum information of to-be-detected blood of a human body by adopting a transmission and reflection manner, and establishing a to-be-tested sample set; and denoising the obtained to-be-tested sample set by using a wavelet analysis method; and obtaining an alcohol concentration predicted value of detecting the blood of the human body by using the to-be-tested sample set as the input of the optical calibrating model. The system comprises a laser emitting module, a signal receiving module, a signal processing module and a digital display module which are sequentially connected. Compared with the prior art, the method has the advantages of rapidness, real time, noninvasive effect and the like.

The invention relates to an automatic classification detecting method for multiple analytes based on the surface enhancement Raman scattering technology. According to the automatic classification detecting method, a nanogold mixed solution formed by integrating three water-soluble polymers which contain Raman active molecules on the surfaces and are capable of specifically recognizing the target analytes serves as a probe, a water solution containing Pb2+ and a water solution containing Ag+ and a water solution containing Cu2+ are mixed with the nanogold mixed solution respectively or at the same time, detecting is carried out through enhancement of a Raman scattering spectrum, efficient automatic classification selectivity for Pb2+, Ag+ and Cu2+ is achieved through the detection, and therefore the three ions and corresponding contents of the three ions can be identified at the same time. According to the automatic classification detecting method, operation is easy and convenient, sensitivity is high, and wide application prospects are achieved in the fields such as biomedicine and environment monitoring.

The invention discloses a single-particle detection system based on Raman and laser-induced breakdown spectroscopy integration. The single-particle detection system comprises an objective table, an illumination unit, a microscopic imaging unit, a Raman detection unit and a laser-induced breakdown spectroscopy detection unit. Single particles are placed on the objective table, the positions of thesingle particles can be precisely adjusted in a two-dimensional mode, and the microscopic imaging unit collects images of the single particles. The Raman detection unit comprises a continuous operation laser device and a first spectrograph, the continuous operation laser device irradiates the surfaces of the single particles, excites the vibration mode of molecules contained in the single particles and generates scattered photons with the frequency different from that of the exciting light, and the first spectrograph records a Raman scattering spectrum to analyze molecular information of the single particles. The laser-induced breakdown spectroscopy detection unit comprises a pulse laser and a second spectrograph, the pulse laser generates high-energy nanosecond pulses, and a high-intensity electric field of the pulse laser instantly ionizes particles to be detected and generates plasma to emit light; and the second spectrograph is used for detecting plasma luminescence and analyzing the element components of the to-be-detected single particles.

The invention discloses a cerebrospinal fluid cell detection method and system based on a Raman scattering spectrum. The method comprises the following steps: 1, preparing a cerebrospinal fluid sample; 2, carrying out single-point test and imaging test on cerebrospinal fluid cells on the sample to obtain original Raman spectrum data; 3, processing the original Raman spectrum data obtained in the step 2 to obtain processed Raman spectrum data, wherein the processing comprises the steps of substrate removal preprocessing and normalization processing; and 4, carrying out data analysis on the Raman spectrum data processed in the step 3 by adopting a deep neural network and principal component analysis, and gathering the spectrums of cerebrospinal fluid cells of the same type together with datanodes of the same color to finish classification and detection. According to the method and the system, the detection and judgment accuracy can be improved, and meanwhile, the report issuing efficiency can be improved.

The invention discloses a noninvasive blood sugar detection device based on a raman scattering spectrum. The noninvasive blood sugar detection device based on a raman scattering spectrum comprises a semiconductor laser, an optical fiber beam splitter, an optical fiber probe, a light filter, a raman spectrometer, a first circuit switch, a second circuit switch, a phase locking amplifier, a heart rate measuring apparatus and a computer. According to the noninvasive blood sugar detection device based on a raman scattering spectrum disclosed by the invention, raman scattering light contains rich molecular vibration information, the characteristics that the raman scattering spectrum having different molecules has different features are utilized, the raman scattering spectrum is used for recognizing molecules; and through the relative value of a glucose characteristic peak at the position of 1125cm-1 in the raman scattering spectrum in pure blood to the hemoglobin characteristic peak value at the position of 1549cm-1, and real-time human body blood sugar concentration data measured by a blood sugar biochemistry instrument, a standard straight line L of the relationship between the relative peak value of the glucose characteristic peak at the position of 1125cm-1 and the human body blood sugar concentration is constructed, and finally noninvasive real-time detection of the human bodyblood sugar is realized. The noninvasive blood sugar detection device disclosed by the invention has the advantages of being high in precision, pollution-free, harmless to human bodies and the like, and has great application prospects in the field of biological noninvasive detection.

Surface enhanced Raman scattering (SERS) spectra of 4-mercaptobenzoic acid (4-MBA) self-assembled monolayers (SAMs) on gold substrates is presented for SAMs onto which gold nanoparticles of various shapes have been electrostatically immobilized. SERS spectra of 4-MBA SAMs are enhanced in the presence of immobilized gold nanocrystals by a factor of 10⁷-10⁹ relative to 4-MBA in solution. Large enhancement factors are a likely result of plasmon coupling between the nanoparticles (localized surface plasmon) and the smooth gold substrate (surface plasmon polariton), creating large localized electromagnetic fields at their interface, where 4-MBA molecules reside in this sandwich architecture. Moreover, enhancement factors depend on nanoparticle shape, and vary by a factor of 10².

The invention relates to the technical field of biosensors, in particular to a biosensor for detecting ATP (triphosadenine) activity based on surface-enhancement Raman scattering generated by nanogoldparticle aggregation. By means of the characteristic that ATP can be specifically combined with a nucleic acid aptamer of the ATP, Walker Chains of the surfaces of the nanogold particles are released, so that shorter Track Chains on the surfaces of nanogold are catalytically hydrolyzed by means of nuclease, and therefore, the nanogold particles lose nucleic acid protection and are aggregated in ahigh salt buffer solution to generate a surface plasma resonance effect, thereby enhancing the electromagnetic field intensity of the surfaces of the nanogold particles greatly. A Raman dye marked onthe surfaces of the nanogold particles generates a surface-enhancement Raman scattering (SERS) effect and Raman spectra are shown in special positions. When a reaction solution does not contain ATP,Protect Chains cannot be replaced, so that a follow-up nanogold aggregation reaction cannot be carried out, and therefore, no Raman scattering spectra are generated. The biosensor detects the ATP activity quickly, sensitively and safely by taking nucleic acid chains modified on the surfaces of the nanogold particles.

The invention relates to the technical field of biological medicine, in particular to a method for rapidly and effectively detecting conformational change in the aptamer and ligand small molecule binding process. The method comprises the following steps: determining the affinity between a selected aptamer and small molecules by applying a biological membrane interference experiment; detecting conformational change before and after the aptamer is combined with the small molecules through a surface enhanced Raman scattering spectrum technology; and visually explaining the binding process of theaptamer and the ligand through molecular dynamics simulation. According to the method, theophylline and the aptamer thereof are taken as examples, it is verified that the method provides new idea forresearch on the reliability of the conformational change of the aptamer and design, modification and screening the high-affinity high-selectivity aptamer, and the method can be applied to the fields of detection, sensing, clinical diagnosis and treatment in a better way.

The invention discloses a preparation method and application of an SERS film substrate based on MXene, and relates to the technical field of SERS spectrums. The method comprises the steps of selectively etching away an Al atomic layer in a precursor MAX material by using lithium fluoride-hydrochloric acid as an etching layering agent; repeatedly cleaning the obtained double-transition metal carbide two-dimensional layered material with deionized water, and centrifuging; dispersing in deionized water, shaking, and carrying out centrifugal suction filtration; and obtaining the novel self-supporting SERS film substrate. The preparation method disclosed by the invention is simple to operate and low in cost, has the advantages of high sensitivity, good Raman enhancement effect, high reproducibility and the like, can be applied to trace detection in the fields of environment, food safety, biomarkers and the like, and has a wide application prospect.

The invention discloses an atmospheric Raman-Rayleigh scattering temperature measurement laser radar and an inversion method. The laser radar comprises a laser transmitting unit (1), a receiving telescope (2), a receiving optical fiber (3), a signal detecting unit (4) and a signal processing unit (5). A high-resolution spectrum detection method is adopted to measure Rayleigh scattering spectral signals generated on atmospheric molecules by laser higher than 30km or above and Raman scattering spectral signals generated on atmospheric molecules by laser lower than 30km; and inversion is carried out to obtain the temperature of atmosphere at 30km, or above or below can be obtained based on a characteristic that the Raman scattering spectrum is in direct proportion to the Rayleigh scattering spectrum in echo intensity, and therefore, the temperature measurement space range of the Rayleigh scattering temperature measurement laser radar can be expanded. The atmospheric Raman-Rayleigh scattering temperature measurement laser radar has the advantages of wide temperature measurement height range and small temperature measurement error.

The invention belongs to the technical field of surface-enhanced Raman scattering spectrum analysis. The invention provides a surface-enhanced Raman scattering substrate, and the surface of the surface-enhanced Raman scattering substrate provided by the invention is provided with a multi-stage three-dimensional self-supporting structure of micron pits, nanobelts, nano bulges, nano ripples and nanoparticles. According to the surface-enhanced Raman scattering substrate and the preparation method thereof, by controlling laser processing parameters and scanning parameters, controllable preparation of the micro-nano structure on the surface of the material can be achieved, the surface enhanced Raman scattering effect is further improved, the preparation process is simple and practical, and thesurface enhanced Raman scattering substrate and the preparation method thereof are suitable for batch preparation. The invention also provides application of the surface-enhanced Raman scattering substrate in surface enhanced Raman scattering, and the surface-enhanced Raman scattering substrate provided by the invention has high sensitivity, excellent uniformity and stability, and can be appliedto most detection fields.

The present invention provides a method of detecting nerves, including: step 1 of irradiating a sample with excitation light; step 2 of detecting Raman scattering light from the sample; step 3 of calculating an intensity ratio of a wave number within a specific range of the Raman scattering light detected in the step 2 or extracting a feature of the intensity ratio and subjecting the feature to multivariate analysis and/or statistical analysis; and step 4 of specifically displaying nerves containing unmyelinated nerves, using as an index the intensity ratio or a result from the multivariate analysis and/or the statistical analysis.

The invention discloses a DNA hybridization detection method based on a surface enhanced Raman scattering spectrum technology. The DNA hybridization detection method comprises the following steps: (1)preparing a silver nanoparticle turbid liquid with the diameter of 30 nanometers, (2) adding a sulfhydrylated DNA probe into the turbid liquid prepared in the step (1), (3) adding a DNA sample to bedetected into the silver nanoparticle turbid liquid riveted with the DNA probe in the step (2), and (4) adding a magnesium sulfate solution, negatively-charged silver nanoparticles and a DNA probe into the turbid liquid obtained in the step (3), clustering under the bridging action of divalent magnesium ions to form a sandwich cluster structure, and finally, fixing a centrifugal tube filled with the sandwich cluster structure on a glass tube support for Raman spectrum measurement. According to the method, nondestructive testing of the DNA test article in the DNA measurement process is guaranteed, reliable distinguishing of DNA hybridization signals is achieved, the measurement precision is high, the speed is high, and the cost is low.

The invention discloses a method for rapidly detecting butralin in tobacco through surface enhanced Raman scattering and relates to the technical field of chemical analysis and detection. The method comprises steps of a transparent and flexible nano-gold adhesive tape SERS substrate being prepared; adopting a butralin standard solution to determine a characteristic peak which can be used as a distinguishing basis for detecting butralin by using the surface enhanced Raman scattering spectrum, and determining a linear regression relationship between the butralin concentration and the peak area; pretreating a to-be-detected cigarette sample to obtain a to-be-detected sample solution; and performing Raman spectrum detection on the to-be-detected sample solution by using a portable Raman instrument, and calculating the butralin content in the tobacco according to the peak area of the characteristic peak. According to the method for rapidly detecting butralin in tobacco through surface enhanced Raman scattering, chloroform serves as a micro-extraction agent, glycerol serves as a butralin dispersing agent, butralin in tobacco can enter a hot spot area of an SERS substrate more easily, SERS signals can be increased, and the method has advantages of being high in sensitivity, rapid, easy and convenient to operate and the like.