37 results about "Cerebrospinal fluid" patented technology

Provided herein are methods to treat various neurodegenerative disorders comprising delivering a viral particles to the whole brain, including both superficial and deep structures. In some aspects, the viral particles are administered at low dose levels to the CSF, in conjunction with microbubbles followed by application of focused ultrasound (FUS) to a region of interest of the brain, thereby causing entry of the viral particles to the brain.

The present invention features compositions including sepiapterin, or a pharmaceutically acceptable salt thereof, and methods for the treatment of BH4-related disorders. In some embodiments, these compositions and methods result in an increase in plasma, CSF, and / or brain exposure of sepiapterin.

The application discloses an automatic pressure regulating negative pressure suction device with automatic humidification function, which comprises a tracheal catheter, a closed sputum suction tube connected to the tail end of the tracheal catheter, a humidification tube connected to a humidification tube port on the closed sputum suction tube, an infusion device connected to the upper end of the humidification tube, sterile humidification liquid connected to the infusion device, a quantitative injection device additionally arranged outside a Murphy dropper of the infusion device, and a pressure pump provided with a volume sensor and a closed air bag wrapping the Murphy dropper, wherein the tail end of the closed sputum suction tube is connected to a disposable negative pressure suction liquid storage bottle through a suction device leather strap, and another connecting port of the negative pressure suction bottle is connected to a negative pressure suction pressure regulating table. According to the application, pressure modules are selected according to different suction liquids (sputum, bile, intestinal fluid, cerebrospinal fluid, etc.), a standard negative pressure value range is adjusted by one key, and the application is convenient to use. In addition, the automatic humidification can be performed on thick sputum for the sputum suction operation, the sputum is made to be thin, and the suction is facilitated.

A CSF management method is used with a patient having a body with CSF having a natural flow rate. The method determines determining a target volume for a CSF-containing target compartment of a patient from a volumetric image. The method determines a personalized therapeutic infusion volume of a therapeutic material as a function of the target volume and a total therapeutic infusion volume. A CSF circuit is formed to control flow of CSF in the body. The personalized therapeutic infusion volume is added to the CSF via the CSF circuit at a first time. The therapeutic material is directed, via the CSF, toward the target compartment of the patient. The flow of the CSF in the CSF circuit is adjusted to localize the CSF at the target compartment of the patient.

The present invention relates to a multiplex gene detection system for viral and bacterial infection of the central nervous system, comprising forward and reverse primers for detecting enterovirus, human double echovirus, Mycobacterium tuberculosis, Listeria monocytogenes, Streptococcus pneumoniae, varicella zoster virus, mumps virus, cytomegalovirus, herpes simplex virus type 1, herpes simplex virus type 2, Neisseria meningitidis, Epstein-Barr virus, Streptococcus agalactiae, Haemophilus influenzae, human herpes virus type 6, Escherichia coli K1 and Japanese encephalitis virus, and the detection sample is cerebrospinal fluid. The multiplex gene detection product for viral and bacterial infection of the central nervous system of the present invention has high throughput, low cost, high sensitivity and good specificity, and is used for synchronous identification and semi-quantitative analysis of multiple viral and bacterial infections of the central nervous system.

The invention belongs to the technical field of virus detection, and particularly relates to a double-channel real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection primer, a probe, a detection kit, a detection method and application thereof. The detection sensitivity is high, and the lowest detection limit can reach 10 copies / mL; the detection time period is short, nucleic acid detection can be completed within 45 minutes, and the kit is suitable for clinical and bedside rapid detection and diagnosis; the detection specificity is high, and cross amplification with other 80 pathogens is avoided; the detection accuracy is 100%; virus quantitative analysis can be carried out, and the quantitative linear range is good; the detection repeatability is good, and the precision is high; uDG enzyme is added into a detection system, so that pollution can be prevented; a reference gene is also added into the detection system, so that the quality of the whole extraction and amplification process of the sample can be monitored according to the detection result of the reference gene, and the existence of the RNA of the Enduma virus in samples such as whole blood, serum, plasma, cerebrospinal fluid, urine, alveolar lavage fluid, throat swab and excrement can be detected.

The application discloses an injectable double-layer drug sustained-release hydrogel and a preparation method and application thereof, and relates to the field of drug sustained-release hydrogels. The swelling and degradation curve and mechanical property detection of the hydrogel show that, when the swelling rate ratio of the inner layer hydrogel to the outer layer hydrogel is 14% to 10%, the secondary damage of the optic nerve caused by extrusion can be avoided; meanwhile, the hydrogel has suitable mechanical strength and adhesion to cope with the in-vivo environment. The hydrogel precursor solution has good injectability, and the gelation time can be controlled within 2 minutes, which perfectly matches the short time window of the optic nerve decompression surgery, and has high clinical practical value. The application solves the major surgical risk of cerebrospinal fluid leakage when the optic nerve is locally administered, and solves the problems of the traditional local drug carrier, such as lack of in-situ stability and easy displacement. The application can synergistically regulate the injury microenvironment and promote the long-acting sustained-release of nerve regeneration, successfully integrates multiple functions such as surgical safety, drug long-acting sustained-release, biocompatibility and operation convenience, and provides an unprecedented effective strategy for solving the clinical problem of the optic nerve injury.

A method for estimating a number of phagocytes in a body fluid of an animal includes stimulating NADPH oxidase activity of the phagocytes; and quantifying a resulting reductive dioxygenation of a chemiluminigenic substrate by an emitted chemiluminescence of the chemiluminigenic substrate using an instrument capable of measuring light. The NADPH oxidase activity of the phagocytes is preferably stimulated by an immunologic or chemical capable of activating a respiratory burst by the phagocytes. The NADPH oxidase activity of the phagocytes is preferably stimulated by a stimulus in solution or coated to a surface contacted by the phagocytes. The stimulus is preferably phorbol myristate acetate (PMA). The animal is preferably human, and the body fluid is preferably blood and / or spinal fluid. The phagocytes are preferably neutrophil leukocytes, and the chemiluminigenic substrate is preferably lucigenin (N,N′-dimethyl-9,9′-biacridinium dinitrate). Also provided is a method of treatment based on the estimation method.

The present disclosure relates to a method of reducing urine glycosaminoglycans (GAG) or cerebral spinal fluid (CSF) glycosaminoglycans (GAG) in a subject, comprising: administering a single dose of a population of modified B cells to the subject.

The utility model discloses a cerebrospinal fluid shunt tube and an accessory structure thereof, and particularly relates to the field of medical instruments, the cerebrospinal fluid shunt tube comprises a polypropylene base, a silica gel dome is arranged on the surface of the polypropylene base, a liquid storage bag is arranged in the silica gel dome, an inlet and an outlet are respectively arranged at two ends of the silica gel dome, and speed limiters are arranged on two sides of the silica gel dome. A ray impermeability mark is arranged inside the silica gel dome, a ray impermeability flow arrow is arranged on the surface of the silica gel dome, a ray impermeability pressure point is marked on one side of the ray impermeability flow arrow, the tantalum-impregnated ray impermeability flow arrow is marked on the surface of the silica gel dome so as to indicate the flow direction from the near end to the far end, and meanwhile, the tantalum-impregnated ray impermeability flow arrow is marked on the surface of the silica gel dome so as to indicate the flow direction from the near end to the far end. Radiopaque pressure points are provided to identify the valve pressure using X-rays, and radiopaque indicia on each adapter base allow a doctor to identify the relative position of the in vivo catheter and valve by X-rays when using the radiopaque catheter, so that the doctor views and adjusts the pressure.

A drainage device (10) comprising a drainage circuit (20) and a monitoring device (50) for the drainage circuit (20), wherein: the drainage circuit (20) has a chamber (30) in which a body fluid (60) can be accumulated, and a body fluid introduction path (40) that is positioned in the chamber (30) and has a dripping port (41) through which the body fluid (60) is dripped into the chamber (30); the monitoring device (50) has a sensor unit (51); and the sensor unit (51) is configured to detect the presence of the body fluid (60) in the chamber (30) in a domain (D) above the dripping port (41).

A method for diagnosing neurodegenerative diseases, the method including measuring the JNK3 levels in a biological sample selected from plasma, CSF, and saliva. The method also includes measuring P-JNK3.

Disclosed is a composition for supporting the survival and differentiation of neural progenitor cells (NPCs) transplanted into a site of nerve injury or disease, the composition comprising (a) a gel-forming molecule and (b) a chemokine receptor type 5 (CCR5) antagonist. Also disclosed is a method for treating nerve injury or disease in a subject, the method comprising: (a) mixing NPCs with a composition disclosed herein; and (b) administering the mixture of NPCs and the composition into the site of nerve injury or disease in the subject, thereby supporting the survival and differentiation of NPCs. Also disclosed is the use of a mixture of NPCs and a composition disclosed herein in the manufacture of a medicament for treating nerve injury or disease in a subject, the mixture being administered into the site of nerve injury or disease in the subject, thereby supporting the survival and differentiation of NPCs. Furthermore, disclosed is a kit for use in supporting the survival and differentiation of NPCs transplanted into the site of nerve injury or disease, the kit comprising: (a) a composition disclosed herein, (b) artificial cerebrospinal fluid (α-CSF), (c) CaCl2, and (d) thrombin.

The utility model discloses a lumbar postoperative drainage tube fixing device, and relates to the technical field of medical instruments. The drainage device comprises a fixing band, a drainage tube groove of an inverted-T-shaped structure is formed in the front face of the fixing band, an opening gap is formed in the position, corresponding to the drainage tube groove, of the back face of the fixing band, a plurality of water cushions distributed in a rectangular array mode are fixedly installed on the front face of the fixing band, and a group of drainage ball fixing bags are fixedly installed at the two ends of the fixing band respectively. The device is bound to a wound, the wound can be pressurized and fixed, cerebrospinal fluid is reduced, a drainage ball and a drainage tube penetrate from the back face of the fixing band to the front face of the fixing band through the opening gap, and then the drainage tube connected with the wound is placed in the drainage tube groove to be preliminarily fixed. Then the drainage ball needed to be used for drainage is placed in the drainage ball fixing bag, finally, the drainage tube is connected with the drainage ball, and the drainage ball can be placed by selecting a proper drainage ball fixing bag according to the body position of a patient.

The present invention provides a method and system for processing multiple meningitis data using a machine learning-assisted sensor array, belonging to the field of data processing and sensor array technology. The present invention comprises obtaining a sensor element; synthesizing the sensor element using a metal-organic framework, a covalent organic framework, and a hydrogen-bonded organic framework; constructing a sensor array based on the sensor element; obtaining a fluorescence signal based on the sensor array and meningitis cerebrospinal fluid; and obtaining information on the type of meningitis based on the fluorescence signal and a machine learning algorithm. The present invention enables data processing for different types of meningitis infections.

The present invention belongs to the technical field of artificial cerebrospinal fluid proportioning, and specifically relates to an artificial cerebrospinal fluid proportioning device and a container, comprising an installation box and a mixed gas supply device, wherein the lower end of the installation box is rotatably connected to a mounting column, a solution mixer is symmetrically installed inside the installation column, a circular plate is fixedly connected inside the protective cylinder, the circular plate is fixedly connected to a cylinder, a first spring is fixedly installed at the lower end of the circular plate, an abutment ring is fixedly installed at the lower end of the first spring, an outer gear ring is sleeved on the outer side of the cylinder, the cylinder is threadedly connected to the container, a gear ring cylinder is fixedly connected to the outer side of the installation column, a threaded rod is slidably connected to the middle part of the installation column, a sealing cylinder is fixedly connected inside the installation box, a torsion spring box is installed on the upper side of the inner side of the sealing cylinder, a rotating rod is fixedly connected to the output end of the torsion spring box, and a piston is fixedly connected to the upper end of the threaded rod; the present invention can clean the unused solution mixer during the process of connecting the container to the solution mixer, so as to avoid affecting the subsequent mixing of artificial cerebrospinal fluid after the raw materials are mixed.

The invention belongs to the technical field of photoelectrochemical PEC biosensors, and particularly relates to a PEC sandwich immunosensor for detecting a starch beta-like protein 1-42 fragment A beta 1-42 of an AD biomarker of Alzheimer's disease, sensing test paper and preparation and application of the PEC sandwich immunosensor and the sensing test paper. The sensor or the sensing test paper takes a U-coated C composite material as an immune matrix, is combined with a capture antibody Ab1, and seals uncombined recognition sites by using bovine serum albumin (BSA), so that A beta1-42 is specifically captured and recognized; pDA / P-Z is taken as a signal substance of the immune probe, and is combined with a detection antibody Ab2, and then A beta 1-42 is specifically captured and recognized, so that a U-C-Ab1-BSA-(A beta 1-42)-Ab2-PDA / P-Z sandwich immune complex is formed, and a detection signal is enhanced; the PEC sandwich immunosensor and the PEC-LFIA test strip constructed by the invention can accurately detect trace Abeta1-42 in artificial cerebrospinal fluid and human plasma, and show good linearity and reproducibility.

Various examples are provided for disposable medical sensors that can be used for the detection of cerebral spinal fluid. In one example, a medical sensing system includes a disposable sensing unit comprising a functionalized sensing area disposed between electrodes; and a portable sensing unit analyzer including pulse generation circuitry that can generate synchronized gate and drain pulses and a transistor with a gate electrically coupled to one electrode. A gate pulse output of the pulse generation circuitry is electrically coupled to a second electrode and a drain pulse output is electrically coupled to a drain of the transistor. In another example, a method includes providing a sample to a functionalized sensing area, generating synchronized gate and drain pulses for a transistor, the gate pulse provided via the electrodes and functionalized sensing area, and sensing an output of the transistor that is a function of a target concentration of the sample.

The invention belongs to the technical field of medical instruments, and provides a ventricular drainage device which comprises a totally-closed first drainage pipeline, a buffer bottle is arranged at one end of the first drainage pipeline, the first drainage pipeline is inserted into the buffer bottle in a sealed mode, and a second drainage pipeline is arranged at the position, on one side of the first drainage pipeline, of the bottle bottom of the buffer bottle. A drainage bag is arranged at the other end of the drainage pipeline II; the device further comprises a hollow sterile sampling cavity, the sterile sampling cavity is connected to the outer wall of the buffer bottle in a sealed mode and communicated with the buffer bottle, a fixedly-connected self-sealing silica gel layer is arranged at the position, close to the end, of the interior of the sterile sampling cavity, a containing frame is arranged on one side of the buffer bottle, and the buffer bottle and the drainage bag are installed on the containing frame. According to the cerebrospinal fluid sampling device, the buffer bottle is arranged, the sterile sampling cavity is formed in the buffer bottle, and the self-sealing silica gel layer is arranged in the sterile sampling cavity, so that closed sampling is realized when cerebrospinal fluid is sampled, and compared with a traditional mode, the infection rate is effectively reduced.

Disclosed is a composition for supporting survival and differentiation of neural precursor cells (NPCs) grafted into a neurological injury or disease site, the composition comprising: (a) a gel forming molecule; and (b) a chemokine receptor type 5 (CCR5) antagonist. Also disclosed is a method of treating a neurological injury or disease of a subject, comprising (a) mixing NPCs with the composition as disclosed herein; and (b) administering a mixture of the NPCs and the composition into a neurological injury or disease site of the subject, to thereby support survival and differentiation of the NPCs. Also disclosed is use of a mixture of NPCs and the composition as disclosed herein in the manufacture of a medicament for treating a neurological injury or disease of a subject, wherein the mixture is to be administered into a neurological injury or disease site of the subject, to thereby support survival and differentiation of the NPCs. Further disclosed is a kit for use in supporting survival and differentiation of NPCs grafted into a neurological injury or disease site, the kit comprising: (a) the composition as disclosed herein; (b) artificial cerebral spinal fluid (a-CSF); (c) CaCl2; and (d) thrombin.

Disclosed are compositions and methods for the treatment of hydrocephalus, for example pediatric hydrocephalus. The disclosed methods employ a non-surgical tool that may be used to control CSF production rate by inducing choroid plexus-specific apoptosis. In aspects, the non-surgical tools employ an AAV vector, a promoter, a suicide gene, and a suicide gene activator to achieve targeted choroid-plexus ablation and subsequent reduction of CSF volume.

A method for estimating a number of phagocytes in a body fluid of an animal includes stimulating NADPH oxidase activity of the phagocytes; and quantifying a resulting reductive dioxygenation of a chemiluminigenic substrate by an emitted chemiluminescence of the chemiluminigenic substrate using an instrument capable of measuring light. The NADPH oxidase activity of the phagocytes is preferably stimulated by an immunologic or chemical capable of activating a respiratory burst by the phagocytes. The NADPH oxidase activity of the phagocytes is preferably stimulated by a stimulus in solution or coated to a surface contacted by the phagocytes. The stimulus is preferably phorbol myristate acetate (PMA). The animal is preferably human, and the body fluid is preferably blood and / or spinal fluid. The phagocytes are preferably neutrophil leukocytes, and the chemiluminigenic substrate is preferably lucigenin (N,N′-dimethyl-9,9′-biacridinium dinitrate). Also provided is a method of treatment based on the estimation method.

The invention provides a high-stability anti-ganglioside antibody detection membrane strip and a kit comprising the same, and belongs to the technical field of biological detection. The detection membrane strip comprises a carrier membrane and a ganglioside antigen coated on the surface of the carrier membrane, the surface of the carrier membrane is combined with the antigen through a thiol-Schiff base double-dynamic covalent network, and the network is composed of a Schiff base bond formed by a carrier membrane aldehyde group and an antigen sugar chain amino group, and a disulfide bond formed by a carrier membrane thiol group and an antigen lipid tail thiol group. The directional fixation of the antigen can be realized, and the dynamic repair capability is realized; a cascade anti-oxidation system is embedded into the network, so that the antigen stability is synergistically improved. The kit containing the membrane strip can be used for detecting the anti-ganglioside antibody in serum, plasma or cerebrospinal fluid, and solves the problems of short period of validity, large batch difference and the like caused by the fact that an existing detection membrane strip is easy to oxidize antigens and poor in coating stability.

The invention discloses a targeted sequencing method for pathogenic microorganisms, and belongs to the technical field of gene detection.The targeted sequencing method comprises the following steps that free nucleic acid in a to-be-detected sample is extracted and subjected to fragmentation treatment, a targeted sequence of the pathogenic microorganisms is specifically captured after a library is constructed, then the captured sequence is enriched with magnetic beads, and a target sequence is obtained; eluting the enriched product, and removing a sequence which is not specifically bound; amplifying the obtained specific capture sequence by using a universal primer to obtain an amplification product; and sequencing the amplification product on a machine, and removing a host sequence by a bioinformatics analysis method to obtain gene information of the pathogenic microorganisms. According to the method, the pathogen detection sensitivity of a sample in which pathogenic microorganisms mainly exist in the form of free nucleic acid is improved, the sequencing data volume is effectively reduced, and the method has relatively high application value on pathogen detection of samples in which microorganisms such as blood and cerebrospinal fluid exist in the form of free nucleic acid.

The present disclosure relates to a method of reducing urine glycosaminoglycans (GAG) or cerebral spinal fluid (CSF) glycosaminoglycans (GAG) in a subject, comprising: administering a single dose of a population of modified B cells to the subject.

The invention discloses a neurosyphilis diagnosis and prediction system and method based on XGBoost, and the system comprises a data input module, a data preprocessing module, a model prediction module, a result visualization and explanation module, a user management module and a data storage module which are electrically connected in sequence. The data preprocessing module is configured to perform missing value filling and abnormal value processing on original data, a dual-prediction model based on an XGBoost algorithm is built in the model prediction module, and the dual-prediction model is constructed to cover different clinical scenes: Model 1 aims at a complete diagnosis and treatment scene of cerebrospinal fluid indexes, Model 2 aims at a basic-level cerebrospinal fluid index missing scene, and Model 3 aims at a basic-level cerebrospinal fluid index missing scene. By integrating multi-region diagnosis guide core indexes, utilizing multi-center data to train an optimization model and introducing an interpretability module, non-invasive, precise and high-consistency auxiliary diagnosis of neurosyphilis is realized, unnecessary lumbar puncture operation is reduced, and clinical diagnosis efficiency is improved.

The utility model discloses a brain tissue pathology sampling device which comprises a refrigeration groove with a first cavity and a second cavity, the first cavity is located below the second cavity, refrigerant is arranged in the first cavity, artificial cerebrospinal fluid is arranged in the second cavity, and a liquid inlet and a liquid outlet are formed in the side wall of the second cavity. The activity of tissue cells can be kept through the low-temperature environment and the artificial cerebrospinal fluid, and then the single cell sequencing quality is improved.

This invention relates to the field of medical device technology and discloses an easily adjustable intelligent drainage device for critically ill neurosurgical patients. The device includes a drainage tube, a collection bag, a control box, a flow rate regulator, and a magnetically self-locking reflux device. The drainage tube includes an inlet end near the patient's cranium and an outlet end connected to the collection bag. The flow rate regulator and the magnetically self-locking reflux device are positioned between the inlet and outlet ends. The control box contains a main control module, a display module, and a storage module. The collection bag is specifically a sterile inner bag with a miniature patch-type liquid level sensor. The sterile inner bag is connected to the outlet end. An infrared drip rate sensor is installed on the outer wall of the inlet end. Both the liquid level sensor and the infrared drip rate sensor are electrically connected to the main control module. This invention achieves intelligent and precise drainage of cerebrospinal fluid, prevents reflux and blockage, provides automatic monitoring and early warning, reduces complications, alleviates the burden on medical staff, and improves drainage safety and efficiency.

The invention discloses a centrifugal machine and application thereof, the centrifugal machine comprises a main body frame, a partition plate is arranged in the middle of the main body frame, and the centrifugal machine is divided into a centrifugal chamber and an accessory chamber by the partition plate; the rotor assembly is arranged above the partition plate, is positioned in the centrifugal chamber and is used for placing a sample; a refrigeration assembly is arranged on the outer wall of the centrifugal chamber and used for cooling or refrigerating the centrifugal chamber; the positioning assembly is arranged below the partition plate and located in the accessory chamber. When the centrifugal machine is used for carrying out separation and enrichment treatment on infectious samples, most of human cells, proteins, saccharides, salts and other impurities in uncultured or cultured clinical samples such as blood, cerebrospinal fluid, urine, hydrothorax and ascites and the like can be effectively removed, and relatively pure living pathogens are obtained.