9results about How to "Easy to repeat" patented technology

The invention provides a construction method of a non-small cell lung cancer histone H1.3 arginine methylation point mutation cell model. The construction method comprises the following steps: designing mutation primers H1.3 R80A-F and H1.3 R80A-R; the method comprises the following steps: by taking a pCDH-HA-H1.3-Flag plasmid as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting a mutation primer, digesting a product by DMT enzyme, and converting a competent cell to obtain a mutant plasmid; and co-transfecting the mutant plasmid and a helper plasmid to a packaging cell, collecting a virus solution, filtering, infecting an A549 cell, adding Polybrene to assist infection, culturing, and screening a stably transfected cell strain by using puromycin to obtain the recombinant plasmid. The invention also provides application of the mutant cell model obtained by the construction method. According to the invention, the blank of histone H1.3 methylation research is filled, the 80th arginine is clear as a core modification site, and the established model provides a new tool for lung cancer mechanism research and drug research and development.

The application discloses a double-array resistivity hole pressure static sounding device, which comprises a conical probe, a hole pressure filter ring, a sidewall upper friction cylinder, a resistivity testing module, a digital circuit board and an analog-digital converter; the conical probe is connected with the sidewall upper friction cylinder through the hole pressure filter ring; the sidewall upper friction cylinder is connected with the resistivity testing module; the sidewall upper friction cylinder is internally provided with a hole pressure sensor, a cone tip resistance sensor, a side friction resistance sensor, a temperature compensator and an inclinometer which are connected with the digital circuit board; the digital circuit board is connected with the resistivity testing module; the digital circuit board is connected with the analog-digital converter through a coaxial cable; the coaxial cable is connected with the analog-digital converter and a data acquisition instrument outside the device; the soil body horizontal resistivity measured by the application has the characteristics of in-situ, rapid, continuous and reliable, and provides a powerful detection tool for geotechnical engineering practice.

The utility model discloses a kind of pickling boxes, including box body, sealing cover, piston and detachably installed on the vacuumizing assembly of sealing cover, box body is equipped with containing cavity;Sealing cover is used to cover on box body, sealing cover is equipped with first sealing ring and is opened with through-hole;Piston is rotatably clamped in through-hole, when piston rotates to first angle position, box body is in storage mode, when piston rotates to second angle position, box body is in pickling mode;When box body is in pickling mode, vacuumizing assembly is connected with sealing cover and the air in containing cavity is extracted, to make piston press down and block first through-hole, to form vacuum state in containing cavity. Thus, by setting rotatable piston on sealing cover, so that container can be freely switched between storage mode and pickling mode, realize a box multi-use, it can be used as ordinary sealed storage box, also can be switched to pickling mode by vacuumizing assembly, greatly improve the flexibility and application range of use.

The invention discloses a preparation method of a doped and wrapped film structure, and belongs to the technical field of film preparation. Comprising the following steps: S1, cleaning a substrate material and feeding the substrate material into a sputtering chamber; s2, taking a ZnS target and a ZnO target as sputtering target materials, and sequentially carrying out the following coating steps on the substrate by using magnetron sputtering equipment: (a) vacuumizing a sputtering chamber to 1.0 * 10 <-4 > Pa, and introducing argon to carry out pre-sputtering; (b) sputtering to form a first ZnS layer; (c) sputtering to form a ZnO layer; (d) performing co-sputtering of ZnS and ZnO to form a co-doped layer; (e) sputtering to form a second ZnS layer; and S3, placing the coated sample in an oxygen atmosphere, and carrying out high-temperature annealing treatment to obtain the doped and wrapped film structure. The preparation method is simple and controllable in preparation process, good in stability and suitable for the window layer of the efficient thin-film solar cell.

This invention provides a method for preparing high-sphericity gold powder, relating to the field of nanomaterial preparation technology. The method involves sequentially adding a multi-ligand stabilizer solution and an acidic solution to a chloroauric acid solution, adjusting the pH of the reaction system to 4.0-5.0 to obtain solution A. Then, a dispersant solution is rapidly added to solution A for a first-stage mixing, followed by the rapid addition of a reducing agent solution for a second-stage mixing. Subsequently, a morphology-optimizing agent solution is added dropwise to the reaction system, and after sufficient reaction, the precipitate is collected to obtain high-sphericity gold powder. This invention achieves precise control of gold powder morphology through the competitive adsorption between ligands by adding a multi-ligand stabilizer to the chloroauric acid solution. Furthermore, the addition of an acidic solution to the chloroauric acid solution further consolidates the morphology control of the gold powder. The acidic pH of the solution after adjustment further slows down the reduction rate, resulting in better dispersibility and more uniform size of the gold powder.

The application discloses a process for preparing epichlorohydrin by directly oxidizing chloropropene, which comprises the following steps: feeding a homogeneous mixture containing chloropropene, hydrogen peroxide, polyhydric alcohol and non-ionic surfactant into a continuous reactor to perform reaction, and obtaining a product containing epichlorohydrin. Compared with the traditional method, the reaction byproduct is less, the product after hydrogen peroxide reaction is water, the non-ionic surfactant and polyhydric alcohol are added to promote the mixing of the organic phase and the aqueous phase into a homogeneous phase, improve the selectivity, a large amount of solvent does not need to be added, solvent evaporation recovery and other processes are avoided, the process is energy-saving and environment-friendly, the equipment process is simple, the yield and selectivity are high, the process is suitable for large-scale industrial production, has great economic benefits, solvent evaporation and recovery are not needed, is beneficial to industrial production amplification, the equipment maintenance cost is low, the system cost is relatively low, the environmental pollution is small, the operation is simple, the process is easy to repeat, and epichlorohydrin can be efficiently produced.

This invention belongs to the field of polymer material preparation and nanomaterial processing technology, specifically relating to a method for preparing polyvinyl alcohol (PVA) nanoparticles. The method for preparing PVA nanoparticles provided by this invention includes the following steps: mixing PVA powder raw material with hot water to obtain a hot aqueous solution of PVA; adding the hot aqueous solution of PVA dropwise to pretreated acetone under stirring conditions to induce precipitation and obtain a suspension; performing solid-liquid separation on the suspension, and drying the resulting solid phase to obtain the PVA nanoparticles. This preparation method uses commercially available PVA as raw material and is simple, mild, and easily repeatable.

The invention discloses a preparation method of a flower-like CuCo2O4 spinel catalyst for CO and NOX synergistic purification. The method comprises the following steps: weighing a copper salt and a cobalt salt according to a molar ratio of Cu / Co of 1: 2, dissolving in water, ultrasonically stirring uniformly, adding an alkaline solution to adjust the pH value of the mixed solution, stirring to alkalescence, transferring to a hydrothermal autoclave, carrying out hydrothermal reaction, carrying out suction filtration and drying on the reaction product to obtain a catalyst precursor, and drying to obtain the catalyst. And roasting in air to obtain the flower-like CuCo2O4 spinel catalyst which is small in particle size and rich in pore channel and structure defect microscopic characteristics. The process is simple and easy to repeat, large-scale industrial preparation can be achieved, and in addition, the catalyst has low cost and high catalytic activity, is suitable for the environmental catalysis fields of gas purification, automobile industry tail gas treatment and the like, and has good industrial application prospects.