Preparation method of mesoporous silica fluorescent nanoparticles for pH ratio probes

A mesoporous silica and fluorescent nanotechnology, applied in the field of advanced nanomaterials and sensors, can solve the problems of easy agglomeration of hydrogels, limited application of in-depth research, and high biological toxicity of quantum dots, and achieve good biocompatibility, Mild reaction conditions and reproducible results

Inactive Publication Date: 2011-03-30
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, it is well known that quantum dots have great biological toxicity and cannot be applied to biological systems. These shortcomings limit their application and further in-depth research
It has also been reported that the PH ratio probe was prepared by coating FRET dyes with nano-hydrogels (Angew.Chem.Int.Ed.2010, 49, 4246-4249), but the hydrogels are easy to agglomerate, and under certain environmental conditions the water The gel is also easy to decompose, which greatly affects the accuracy of the test

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  • Preparation method of mesoporous silica fluorescent nanoparticles for pH ratio probes
  • Preparation method of mesoporous silica fluorescent nanoparticles for pH ratio probes
  • Preparation method of mesoporous silica fluorescent nanoparticles for pH ratio probes

Examples

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Embodiment 1

[0036] A method for preparing mesoporous silica fluorescent nanoparticles for pH ratio probes, the method comprising the following steps:

[0037] (1) Weigh 4.7 mg of FITC and add it to a single-necked small flask, add a small magnet, add 1.136 ml of absolute ethanol, then add 50 μl of APTS, seal and protect from light for 12 hours to prepare the FITC-APTS complex;

[0038] (2) Weigh 6.3 mg of RBITC and add it to a single-necked small flask, add a small magnet, add 1.136 ml of absolute ethanol, then add 50 μl of APTS, seal and protect from light for 12 hours, and prepare the RBITC-APTS complex;

[0039](3) Weigh 200mg CTAB, add it into a three-neck flask, add 100ml secondary water, add 0.7ml 2mol / L sodium hydroxide solution, stir for 15min, heat up to 70°C, add 1ml TEOS, 80μl FITC-APTS, 20μl RBITC -APTS, add 1ml of ethyl acetate after 1min, stir for 30s, and let stand for 2h. The sample is cooled, centrifuged at 12000rpm, redispersed into secondary water, and centrifuged, so ...

Embodiment 2～7

[0042] The preparation method was the same as in Example 1, except that the mixing ratio of FITC:RBITC two dyes was different, as shown in Table 1, and mesoporous silica fluorescent nanoparticles were prepared.

[0043] Table 1

[0044]

[0045] When mesoporous silica fluorescent nanoparticles are prepared according to the above examples, the pH detection range and detection sensitivity of the nanoparticles can be adjusted by adjusting the ratio of the two dyes: when the amount of FITC incorporated is large, the nanoparticles It has a better linear detection effect in the acidic range; when the amount of RBITC incorporated is larger, it has a better linear detection effect in the alkaline range; when the ratio of the two is 1:2, it has the widest pH detection range. Simultaneously, along with the increase of FITC amount, the sensitivity of detection improves, and when FITC:RBITC=1:4, its linear slope is 0.41, and promptly the fluorescence ratio of the two changes 0.41 every...

Embodiment 8

[0049] The mesoporous silica fluorescent nanoparticle used for the pH ratio probe proposed by the present invention is a functional nanoparticle doped with binary fluorescence resonance energy transfer (FRET) dye pairs, and its particle size is 200- 1 μm. Due to the introduction of organic fluorescent dye molecules during the preparation process, the nanoparticles can emit fluorescence under visible light excitation; due to the large overlap between the excitation and emission spectra of the two introduced organic fluorescent dye molecules, it can constitute a fluorescence resonance energy The dye pair is transferred, so two kinds of fluorescence emission can be produced under the excitation of a single wavelength; one of the fluorescence emission is sensitive to pH, and the other is stable to pH, and the ratio of the fluorescence intensity of the two changes linearly with the pH value, so the The nanoparticles can be used as pH ratio probes; at the same time, the nanoparticle...

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Abstract

The invention relates to a preparation method of mesoporous silica fluorescent nanoparticles for pH ratio probes, which comprises the following steps of: firstly adopting silane coupling agents for carrying out pre-modification on a binary fluorescence resonance energy transfer dye; then, by using a sol-gel chemical principle and the self assembling behavior of silicon materials and surface active agents used as structural guide agents in solution, obtaining the spherical mesoporous silica fluorescent nanoparticles through the co-hydrolyzation fasculation between the silane coupling agents and silicon source precursors. Compared with the prior art, the preparation method has the advantages that the obtained mesoporous silica fluorescent nanoparticles have the single-wavelength exciting pHratio fluorescent probe function, the pH detection limit and the detection range can be regulated and controlled through changing the proportion among the binary fluorescence resonance energy transfer dyes, and in addition, guest molecules can be loaded at high capacitance because the system has good mesoporous structures and biocompatibility.

Description

technical field [0001] The invention relates to the technical field of advanced nanometer materials and sensors, in particular to a method for preparing mesoporous silicon dioxide fluorescent nanoparticles used for pH ratio probes. Background technique [0002] The progress of chemical reactions and many important physiological processes of cells and organelles are closely related to pH value, so the accurate measurement of pH value is very important for chemical biology research. The change of fluorescence or light absorption properties of many organic compounds with pH can be used to indicate the change of acidity and alkalinity in the target medium, but this pH measurement method based on the change of optical signal is easily affected by photobleaching, sensing molecule concentration, sensing Molecular microenvironment (polarity, pH, temperature, instrument), luminescent stability and other factors affect the accurate qualitative and quantitative to a certain extent, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/59C09K11/06G01N21/64
Inventor 张金龙王灵芝雷菊英
Owner EAST CHINA UNIV OF SCI & TECH
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