Preparation method of mesoporous silica fluorescent nanoparticles for pH ratio probes

A mesoporous silica and fluorescent nanotechnology, applied in the field of advanced nanomaterials and sensors, can solve the problems of easy agglomeration of hydrogels, limited application of in-depth research, and high biological toxicity of quantum dots, and achieve good biocompatibility, Mild reaction conditions and reproducible results
CN101993693AInactive Publication Date: 2011-03-30EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2011-03-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method of mesoporous silica fluorescent nanoparticles for pH ratio probes, which comprises the following steps of: firstly adopting silane coupling agents for carrying out pre-modification on a binary fluorescence resonance energy transfer dye; then, by using a sol-gel chemical principle and the self assembling behavior of silicon materials and surface active agents used as structural guide agents in solution, obtaining the spherical mesoporous silica fluorescent nanoparticles through the co-hydrolyzation fasculation between the silane coupling agents and silicon source precursors. Compared with the prior art, the preparation method has the advantages that the obtained mesoporous silica fluorescent nanoparticles have the single-wavelength exciting pHratio fluorescent probe function, the pH detection limit and the detection range can be regulated and controlled through changing the proportion among the binary fluorescence resonance energy transfer dyes, and in addition, guest molecules can be loaded at high capacitance because the system has good mesoporous structures and biocompatibility.
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Description

technical field

[0001] The invention relates to the technical field of advanced nanometer materials and sensors, in particular to a method for preparing mesoporous silicon dioxide fluorescent nanoparticles used for pH ratio probes. Background technique

[0002] The progress of chemical reactions and many important physiological processes of cells and organelles are closely related to pH value, so the accurate measurement of pH value is very important for chemical biology research. The change of fluorescence or light absorption properties of many organic compounds with pH can be used to indicate the change of acidity and alkalinity in the target medium, but this pH measurement method based on the change of optical signal is easily affected by photobleaching, sensing molecule concentration, sensing Molecular microenvironment (polarity, pH, temperature, instrument), luminescent stability and other factors affect the accurate qualitative and quantitative to a certain extent, whi...

Claims

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