C-reactive protein detection reagent and SERS detection method

Abstract

The invention belongs to the field of bio-analytical chemistry, and discloses a C-reactive protein detection reagent and an SERS detection method. The detection reagent is formed by an Au-NNPs probe modified by a sulfhydrylation CRP aptamer and an AgMNPs magnetically capturing substrate modified by an amination CRP aptamer. When CPR exists, the Au-NNPs probe and the AgMNPs magnetically capturing substrate are combined together to form a sandwich structure through a specific recognition effect of the aptamers, an obtained intensity value of a Raman spectrum characteristic peak of a CRP sample is substituted into a "linear curve of the Raman intensity and the CRP concentration" after magnetic separation with the change of the CRP concentration, and the CRP concentration can be obtained through calculation. The method is advantageous in that the signals are stable, the ultra-sensitiveness is achieved, the selectivity is high, and the obtained detection reagent and the method have important application values in clinical analysis and diagnosis.

Description

technical field [0001] The invention belongs to the field of bioanalytical chemistry, and in particular relates to a C-reactive protein detection reagent and a SERS detection method. Background technique [0002] Disease biomarkers are generally certain characteristic biochemical indicators in a common physiological or pathological or therapeutic process that can be objectively measured and evaluated. Through its measurement, the progress of the body's current biological process can be known. Examining a disease-specific biomarker can help in disease identification, early diagnosis and prevention, and monitoring during treatment. [0003] C-reactive protein (CRP) is a pentameric protein with a molecular weight of about 115KD. CRP is synthesized by liver cells, which can activate complement and promote phagocytosis of granulocytes and macrophages. [0004] The traditional view is that CRP is a non-specific inflammatory marker, but research in the past decade has revealed th...

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Problems solved by technology

[0005] The currently reported methods for detecting CRP include electrochemiluminescence, fluorescence, chemiluminescence, surface plasmon resonance, etc., but there are shortcomings such as long detection time and insufficient sensitivity.

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