13 results about "Peptide Aptamers" patented technology

The invention provides an automatic construction method and system of an active polypeptide, and the method comprises the following steps: firstly, determining a target metabolite, and obtaining a target protein having binding activity with the target metabolite; then, obtaining interaction information of the target protein and the target metabolite and secondary structure information of target protein residues; then, inputting the interaction information into a large language model LLM to obtain information of key residues of the target protein; and finally, inputting the structural information of the target protein, the information of the key residues, the secondary structural information and the constraint information into LLM to obtain an active polypeptide capable of being specifically combined with the target metabolite, namely the potential peptide aptamer. According to the method, by combining a biological simulation tool and a large language model, automatic batch generation of the active polypeptide is realized, the acquisition process of the polypeptide is greatly simplified, and a new strategy and a new way are provided for metabolite peptide aptamer design.

This disclosure provides peptide aptamers, comprising one or more inhibitory amino acid sequences, that block human Cav3.2 T-type calcium channel activity and nociceptive dorsal root ganglion (DRG) neuron excitation. Also provided are pharmaceutical compositions comprising the peptide aptamers, nucleic acid constructs encoding the peptide aptamers, and methods of treatment comprising administering the peptide aptamers.

Provided herein are methods of making a peptide aptamer library of partially random peptide aptamers. The peptide aptamer library can be designed with tryptic and chymotryptic sites spaced to create domains. In each domain, a defined subset of amino acids are randomly ordered alongside invariant amino acids. Also provided herein are methods to identify peptide aptamers that bind a target using the peptide aptamer library disclosed herein.

The invention relates to a positively charged peptide capable of non-covalently interact with negatively charged extracellular vesicles or nanoparticles. Said peptide is a fragment of the bacterial protein P40 and shows better interaction capabilities that the entire bacterial protein P40. The peptide of the invention can also be linked to a targeting element, such as targeting peptide, DNA aptamer, RNA aptamer, a protein, an antibody or a fragment thereof that binds to a target molecule, therefore it can be used for the targeted delivery of extracellular vesicles or nanoparticles further comprising a bioactive agent to a target cell, tissue or particle like a virus, that comprise the target molecule. Therefore, the present invention also relates to a carrier complex comprising the peptide of the invention non covalently bound to an extracellular vesicle or nanoparticle comprising a bioactive agent and its uses.

The present invention relates to a Ses i 2-specific binding polypeptide of sesame allergen and a screening method thereof. The specific binding polypeptide includes the polypeptides shown in SEQ ID NO.1-5. The method includes the following steps: (I) Activate and culture Escherichia coli strain 2738, add phages with gradient dilution to obtain a recombinant phage library; (II) Perform three rounds of panning with Ses i 2 protein as the target, amplify the bound target phages, sequence them with 96gIII sequencing primers, and obtain a polypeptide specifically binding to Ses i 2 protein after translation; (III) Synthesize the polypeptide obtained in the above steps and verify the affinity by bio-layer interferometry. The screening method uses a phage library to screen for peptide aptamers of sesame protein Sesi 2, which have high affinity and specificity and can be used for the detection of Ses i 2 protein.

The present application relates to the use of a peptide aptamer comprising a variable peptide segment as shown in SEQ ID NO: 1 or comprising an amino acid sequence produced by substitution, deletion and / or addition of one or several amino acids from the amino acid sequence shown in SEQ ID NO: 1 in the preparation of a biological preparation, a pesticide or a protective agent for inhibiting, resisting or preventing a plant disease caused by G. cingulata; wherein the amino acid sequence produced by substitution, deletion and / or addition of one or several amino acids retains the activity or function of the variable peptide segment. The peptide aptamer of the present application shows obvious inhibitory effect on G. cingulata, can effectively inhibit the germination of spores, and can effectively prevent and control the infection of G. cingulata on the leaves of Stachys floridana, and reduce the infection symptoms of G. cingulata; at the same time, it does not produce toxic side effects on the leaves of Stachys floridana.

The present invention relates to methods of engineering and identifying a peptide aptamer that binds to a target protein of interest, and peptide aptamers engineered and identified using these methods and methods to identify a candidate peptide or nucleic acid that binds to a target protein in a live cell. The peptide aptamers defined herein may be useful for treating a condition associated with dysregulated cap-dependent translation, dysregulated DNA replication, dysregulated DNA repair and / or dysregulated mRNA translation such as cancer, diseases associated with a viral infection and obesity.

Provided herein are methods of making a peptide aptamer library from naturally occurring proteins and / or peptides. The peptide aptamer library can be generated from immunoglobulins, B cell receptors and / or T cell receptors of a host animal. Biological samples such as cells and biofluids can be used as a library of peptide aptamers. Also provided herein are methods to identify peptide aptamers that bind a target using the peptide aptamer library generated by the methods disclosed herein.

Disclosed herein are polypeptide aptamers comprising peptide aptamers that functionally interact with Nav1.7 voltage gated sodium channels, polynucleotides and infectious particles encoding the polypeptide aptamers, and methods of treating diseases and conditions, such as neuropathic pain, using the same.

The invention relates to the technical field of biomarker detection, in particular to an MF-peptide, application, an MF-peptide aptamer electrochemical sensor and an A beta aggregate detection method. The amino acid sequence of the MF-peptide is shown as SEQ ID NO.1, and the MF-peptide is used for detecting A beta aggregate. According to the present invention, the MF-peptide is adopted as the aptamer, the MF-peptide is self-assembled on the gold nanoparticle modified electrode surface through the Au-S bond to obtain the MF-aptamer electrochemical sensor, and the Abeta aggregate in the human blood or the solution can be detected by using the MF-aptamer electrochemical sensor; the sensor and the detection method have the advantages of simple operation, rapid response, high sensitivity and high selectivity.

The application discloses a peptide aptamer for tryptophan detection and a colorimetric tryptophan detection method based on the peptide aptamer, wherein the peptide aptamer sequence is shown as SEQ ID NO. 1, and the N terminal of the peptide aptamer is modified by a space unit composed of 1 hemi-cystine. The application combines the advantages of the peptide aptamer, such as high affinity, high selectivity, simple structure and easy operation, with the advantages of the colorimetric detection method, such as rapidness, simple operation and no need of labeling, so that the rapid and sensitive detection of tryptophan can be realized.

Provided herein are methods of making a peptide aptamer library of partially random peptide aptamers. The peptide aptamer library can be designed with tryptic and chymotryptic sites spaced to create domains. In each domain, a defined subset of amino acids are randomly ordered alongside invariant amino acids. Also provided herein are methods to identify peptide aptamers that bind a target using the peptide aptamer library disclosed herein.