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Raman probe and preparation method thereof, and application of Raman probe

A technology of Raman probe and Raman signal, applied in the field of nanomaterials

Active Publication Date: 2019-09-27
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the requirements of detection sensitivity and imaging speed, the enhanced performance of Raman probes in the near-infrared region needs to be improved, and the Raman imaging speed in the near-infrared region also needs to be improved.

Method used

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  • Raman probe and preparation method thereof, and application of Raman probe
  • Raman probe and preparation method thereof, and application of Raman probe
  • Raman probe and preparation method thereof, and application of Raman probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Example 1 Preparation of a Raman probe using 4-nitrobenzenethiol as the Raman signal molecule in the first Raman signal layer

[0110] Step 1: Add 400uL 1nmol / L gold nano-nuclear particles (particle size: 25nm) prepared by the seed growth method into 1mL 0.02mol / L cetyl ammonium chloride solution, centrifuge and redisperse in In 400uL 0.02mol / L cetyl ammonium chloride solution, obtain the gold nano core with cetyl ammonium chloride as stabilizer.

[0111] Step 2: Add the ethanol solution of 20uL10mmol / L 4-nitrobenzenethiol to the gold nano-nuclei obtained in step 1 with cetyl ammonium chloride as a stabilizer, mix and shake respectively (that is, 4-nitrobenzenethiol After 0, 5, 10, 20, 30, 60, and 960 minutes of alcohol adsorption on the gold nano-nucleus, centrifugal separation and redispersion in 200uL 0.1mol / L cetyl ammonium chloride solution were repeated three times to obtain The outer surface of the gold nano-core is decorated with a layer of 4-nitrobenzenethiol ...

Embodiment 2

[0121] Example 2 Preparation of a Raman probe using 3-nitrobenzylthiol as the Raman signal molecule in the first Raman signal layer

[0122] Step 1: Add 400uL 1nmol / L gold nano-nuclear particles (particle size: 25nm) prepared by the seed growth method into 1mL 0.02mol / L cetyl ammonium chloride solution, centrifuge and redisperse in In 400uL 0.02mol / L cetyl ammonium chloride solution, obtain the gold nano core with cetyl ammonium chloride as stabilizer.

[0123] Step 2: Add 20uL of 10mmol / L 3-nitrobenzyl mercaptan ethanol solution to the gold nano-core obtained in step 1 with cetyl ammonium chloride as a stabilizer, mix and shake for 10 minutes, then centrifuge, Redisperse in 200uL0.1mol / L cetyl ammonium chloride solution, repeat three times, obtain and have one deck 3-nitrobenzylthiol Raman signal molecule layer (being the first Raman signal layer) of gold nanoparticles.

[0124] Step 3: Add the gold nanoparticles obtained in step 2 to 4mL 0.05mol / L cetyl ammonium chloride w...

Embodiment 3

[0125] Example 3 Preparation of a Raman probe using 2-mercapto-5-nitrobenzimidazole as the Raman signal molecule in the first Raman signal layer

[0126] Step 1: Add 400uL 1nmol / L gold nano-nuclear particles (particle size: 25nm) prepared by the seed growth method into 1mL 0.02mol / L cetyl ammonium chloride solution, centrifuge and redisperse in In 400uL 0.02mol / L cetyl ammonium chloride solution, obtain the gold nano core with cetyl ammonium chloride as stabilizer.

[0127] Step 2: Add 20uL of 10mmol / L ethanol solution of 2-mercapto-5-nitrobenzimidazole to the gold nano-core obtained in step 1 with cetyl ammonium chloride as a stabilizer, mix and shake for 10 minutes, then centrifuge Separation and redispersion in 200uL 0.1mol / L cetyl ammonium chloride solution, repeated three times to obtain a layer of 2-mercapto-5-nitrobenzimidazole Raman signal molecule layer on the outer surface of the gold nano-core (i.e. the first Raman signal layer) of gold nanoparticles.

[0128] Ste...

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Abstract

The invention discloses a Raman probe. The Raman probe comprises a nanometer core, a first Raman signal layer and a shell layer. The nanometer core is coated with a first Raman signal layer; Raman signal molecules are distributed in the first Raman signal layer; the shell layer has a first layer and a second layer; the first Raman signal layer is coated with the first layer; and the second layer coats the first layer and is provided with a gap. The Raman probe has the advantages of the high Raman signal and the good signal repeatability. The invention also relates to a preparation method and application of the Raman probe. The preparation method is simple, and the Raman probe can be used for the ultra-sensitive and ultra-fast Raman detection and imaging technologies.

Description

technical field [0001] The invention belongs to the field of nanomaterials, and relates to a Raman probe, a preparation method and application thereof. Background technique [0002] Raman spectroscopy is a fingerprint spectrum that characterizes molecular vibrations. Metal nanoparticles produce plasmon resonance under the action of incident light, which greatly enhances the Raman spectrum of molecules adsorbed on the surface of metal nanoparticles, which is called surface-enhanced Raman scattering effect (SERS). In recent years, novel Raman probes combining metal nanoparticles (i.e., SERS substrates) and Raman signaling molecules have attracted increasing attention. By labeling different Raman signaling molecules on metal nanoparticles, ultra-sensitive Raman probes with different signals can be obtained, and it is expected to realize multi-index molecular detection and biological imaging applications. [0003] The traditional probe is to adsorb Raman signal molecules on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B82Y40/00
CPCG01N21/658B82Y40/00G01N2021/656G01N21/65
Inventor 叶坚张雨晴顾雨清
Owner SHANGHAI JIAO TONG UNIV
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