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Saliva diagnostic sensor as well as preparation method thereof and application thereof in detection of hepatitis C

A hepatitis C, sensor technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problem of high detection cost and high school, and achieve the effects of good repeatability, low cost and good accuracy

Inactive Publication Date: 2018-02-16
苏州纳葛诺斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As early as 2001, Ortho Company launched the ELISA detection kit for detecting HCV core antigen. Through the evaluation of a large number of clinical samples, it was proved that HCV can be detected earlier than the detection of HCV antibody, but there is still a certain rate of missed detection, and the detection cost is relatively low. school height

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  • Saliva diagnostic sensor as well as preparation method thereof and application thereof in detection of hepatitis C
  • Saliva diagnostic sensor as well as preparation method thereof and application thereof in detection of hepatitis C
  • Saliva diagnostic sensor as well as preparation method thereof and application thereof in detection of hepatitis C

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Experimental program
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Embodiment 1

[0034] The present embodiment provides a saliva diagnostic sensor comprising a glass substrate, a gold film and a composite nano-volume, the gold film is covered on the glass substrate, the composite nano-volume is dispersed on the gold film, and the composite nano-volume is formed on the surface of the graphene oxide material The in-situ growth of gold nanoparticles curls and wraps the gold nanoparticles to form a nanoscroll structure. Among them, the thickness of the gold film is 300 nm, the length of the composite nanovolume is 4 μm, and the diameter is 50 nm; the thickness of the graphene oxide material is 4 nm; the particle size of the gold nanoparticles is 50 nm.

[0035] The preparation method of the above-mentioned saliva diagnostic sensor is as follows:

[0036] Step 1: Ultrasonic reaction is performed on a liquid phase reaction system containing 15 ml of graphene oxide with a concentration of 0.5 mg / ml, chloroauric acid ions with a concentration of 0.5 mmol / L, and et...

Embodiment 2

[0048] The present embodiment provides a saliva diagnostic sensor comprising a glass substrate, a gold film and a composite nano-volume, the gold film is covered on the glass substrate, the composite nano-volume is dispersed on the gold film, and the composite nano-volume is formed on the surface of the graphene oxide material The in-situ growth of gold nanoparticles curls and wraps the gold nanoparticles to form a nanoscroll structure. Among them, the thickness of the gold film is 200 nm, the length of the composite nanovolume is 3 μm, and the diameter is 100 nm; the thickness of the graphene oxide material is 6 nm; the particle size of the gold nanoparticles is 60 nm.

[0049] The preparation method of the above-mentioned saliva diagnostic sensor is as follows:

[0050] Step 1: Ultrasonic reaction is performed on a liquid-phase reaction system containing 15 ml of graphene oxide with a concentration of 0.2 mg / ml, chloroplatinic acid ions with a concentration of 0.8 mmol / L, an...

Embodiment 3

[0058] This embodiment provides a saliva diagnostic sensor comprising a glass substrate, a copper film and a composite nano-volume, the copper film is covered on the glass substrate, the composite nano-volume is dispersed on the copper film, and the composite nano-volume is in-situ on the surface of the graphene oxide material A nanoscroll structure formed by curling and encapsulating the gold nanoparticles after the growth of the gold nanoparticles. Among them, the thickness of the gold film is 200 nm, the length of the composite nanovolume is 4 μm, and the diameter is 75 nm; the thickness of the graphene oxide material is 5 nm; the particle size of the gold nanoparticles is 40 nm. .

[0059] The preparation method of the above-mentioned saliva diagnostic sensor is as follows:

[0060] Step 1: Ultrasonic reaction is carried out on a liquid phase reaction system containing 15 ml of graphene oxide with a concentration of 0.2 mg / ml, gold ions with a concentration of 0.3 mmol / L,...

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Abstract

The invention relates to the technical field of hepatitis C virus diagnosis, and discloses a saliva diagnostic sensor as well as a preparation method thereof and application thereof in detection of hepatitis C. The sensor comprises a substrate, a metal film and composite nano rolls, wherein the metal film covers the substrate; the composite nano rolls are dispersed on the metal film; the compositenano rolls respectively have a nano roll structure which is formed by enabling metal nanoparticles to grow on the surface of a graphene material in situ, then rolling up the graphene material and enabling the metal nanoparticles to be wrapped in the graphene material. The preparation method of the sensor comprises the steps of enabling a liquid phase reaction system containing the graphene material, metal ions and an ethanol solution to be subjected to an ultrasonic reaction to prepare the composite nano rolls; coating the substrate, with the sputtered metal film, with dispersion liquid of the composite nano rolls, and drying to obtain the saliva diagnostic sensor. Compared with the prior art, the saliva diagnostic sensor has the advantages of simple operation, rapid detection speed and high accuracy.

Description

technical field [0001] The invention relates to the technical field of hepatitis C virus diagnosis, in particular to a saliva diagnosis sensor, a preparation method and its application in detecting hepatitis C. Background technique [0002] Hepatitis C is prevalent worldwide, and people of different genders, ages, and races are susceptible to hepatitis C virus (HCV). HCV belongs to Flaviviridae and Hepacivirusgenus, and its genome is single-stranded positive-strand RNA. HCV gene is easy to mutate, and can be divided into at least 6 genotypes and multiple subtypes at present. According to the statistics of the World Health Organization, the global HCV infection rate is about 2.8%, about 185 million people are infected with HCV, and about 350,000 deaths are caused by HCV infection every year. However, due to the occult nature of HCV infection, most infected persons do not know that they have been infected with HCV. Therefore, the exact global incidence of chronic hepatitis C...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 刘党培刘岩磊陈云生杨蒙
Owner 苏州纳葛诺斯生物科技有限公司
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