33 results about "Hcv core antigen" patented technology

The present invention is directed to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample. The combination immunoassays of the present invention employ a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without interfering with the performance of other reagents such as the capture of anti-HCV antibodies by recombinant HCV antigens.

The disclosure relates to a multi-epitope fusion protein as well as to its use as calibrator and / or control in an in vitro diagnostics immunoassay for detecting HCV core antigen. The multi-epitope fusion protein has two to six different non-overlapping linear peptides present in the amino acid sequence of hepatitis C virus (HCV) core protein, wherein each of the peptides is separated from the other peptides by a spacer consisting of a non-HCV amino acid sequence and having a chaperone amino acid sequence. No further HCV specific amino acid sequences are present in the polypeptide. A further aspect relates to a reagent kit for detecting HCV core antigen containing said multi-epitope fusion protein as calibrator or control or both.

The invention discloses an efficient hepatitis C virus (HCV) core antigen enzyme-linked immunosorbent assay (ELISA) detection kit which mainly comprises a microlon ELISA plate, an anti-HCV-cAg enzyme conjugate, an HCV-cAg standard substance gradient solution, a positive serum control indication sample, a negative serum control indication sample, an HCV-cAg sample diluent, a substrate solution A, a substrate solution B, a stop buffer and 20* concentrated washing liquid. The kit provided by the invention is high in sensitivity, good in precision and strong in specificity.

The invention provides a virus disaggregating agent and a method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen. The virus disaggregating agent is characterized in that each 100ml of disaggregating agent comprises 0.1-1ml of detergent, 0-20g of protein denaturant, 0.01-1ml of reducing agent, 0.1-1ml of fat solvent, and base liquid as remainder amount. The antigen-antibody complex obtained by the neutralization reaction of testing blood sample or antiserum and virus antigen is disaggregated into free components, or virus core antigen is fully exposed and the antigen reactivity of the virus core antigen is remained, thereby remarkably improving the sensitivity by comparing with the prior HCV core antigen detection method; in addition, the detection window period of the antigen is 49 days shorter than that of the anti-HCV antibody averagely, the situation appears within 1-2d after HCV-RNA appears, thereby efficiently shortening the window period of the blood serum before transformation, which is of important signification on improving the detection rate of affected person in the window period and the safety of blood transfusion and blood products. In the invention, the virus disaggregating agent is suitable for the disaggregation of common virus antigen-antibody complex including HCV and HAV (Hepatitis A Virus).

The present disclosure relates to a method for detecting a core polypeptide of a hepatitis C virus (HCV) in a sample from a subject with the steps of (a) contacting the sample with a surfactant comprising a cationic detergent; (b) contacting the sample with a binding compound; and (c) detecting a core polypeptide of the HCV in the sample; wherein step a) is immediately followed by step b). The present disclosure further relates to a method for pre-processing a sample from a subject for detection of an HCV core polypeptide, involving (a) contacting the sample with a surfactant comprising a cationic detergent and, optionally, with an agent inducing a pH shift, immediately followed by (b) contacting the sample with a binding compound. Moreover, the present disclosure further relates to uses, devices, and analytical systems related to aforesaid methods.

The invention provides a hepatitis C virus (HCV) core antigen detection kit. Based on colloidal gold immunoturbidimetry, the kit contains a reagent R2 which is a solution containing gold nanoparticles labeled with an HCV core antigen antibody. The kit is characterized in that particle size of the gold nanoparticles is 62.2nm-79.1nm; and the mass ratio of the gold nanoparticles to the antibody is 50:20-60. The invention also provides a preparation method of the kit. The kit provided by the invention has characteristics of high sensitivity, high specificity, rapid reaction and good stability. No precipitate is generated after a reaction. It is convenient to clean a biochemical analyzer, and service life of the biochemical analyzer is prolonged.

The invention discloses a hepatitis C virus core antigen nucleic acid aptamer, wherein the nucleotide sequence of the aptamer is as indicated in SEQ ID No.1. The invention also discloses the application of the hepatitis C virus core antigen nucleic acid aptamer in preparing a hepatitis C virus (HCV) core antigen detection kit. The aptamer and the HCV core antigen detection kit prepared therefrom in the invention are capable of detecting the core antigen at pg / ml level; the sensitivity of the hepatitis C virus core antigen is greatly improved; therefore, the HCV nucleic acid aptamer and the HCV-cAg detection kit has important clinic diagnosis meaning and wide application prospect for early diagnosis of window-phase infected patients.

The invention discloses a reagent kit for carrying out chemiluminescent enzyme-linked immunodetection on a hepatitis C virus core antigen. The reagent kit comprises a kit body, and an enzyme label plate, a reagent, a non-drying glue sealing strip and an operating instruction manual which are arranged inside the kit body, wherein the enzyme label plate is a chemiluminescent enzyme label plate; each hole of the plate is coated with coated antibodies Mab1and Mab2 with concentration of 5ug / ml; and the reagent comprises a HCV-cAg antienzyme composition, a gradient solution of a HCV core antigen standard product, positive control serum, negative control serum, a pretreatment solution, a diluent of a HCV-cAg sample, a chemiluminescent zymolyte solution A, a chemiluminescent zymolyte solution B and a 20 * concentrated cleaning solution. The positive detection rate of the reagent kit is close to that of PCR; and simultaneously, the reagent kit has the advantages of simple operation, high sensitivity and high specificity, and is suitable for popularization and application in clinic.

The invention relates to a microfluidic paper base chip for detecting an antibody to hepatitis C virus as well as a preparation method of the chip. The microfluidic paper base chip provided by the invention comprises a plurality of micro detection channels, wherein each channel comprises an HCV core antigen, an HCV NS5 antigen, an HCV NS4 antigen, an HCVNS3 antigen as well as the combination thereof. Owing to the multi-channel detection capability, the microfluidic paper base chip can detect a plurality of immune projects synchronously; miniaturized screening and confirming testing are integrated on the microfluidic paper base chip, so that the complex HCV diagnosis process is simplified greatly, and the diagnosis efficiency is improved greatly.