2results about How to "Sensitive detection method" patented technology

The application discloses a method for quality prediction of tinplate cans based on iron ion migration amount, which comprises the following steps: sealing and soaking the tinplate cans filled with acetic acid, and performing temperature acceleration test; sampling on the third day of the temperature acceleration test, and detecting the iron ion migration amount; sampling on the tenth day of the temperature acceleration test, and detecting the iron ion migration amount; and predicting the quality of the tinplate cans based on the iron ion migration amounts on the third day and the tenth day. The application takes the migration amount on the third day as a short-term risk indicator, and takes the migration amount on the tenth day as a key quality determination standard of long-term stability indicator. Based on the detection results of the two time points, the iron ion migration trend of the tinplate cans within a six-month shelf period can be reliably predicted, the sensory quality and stability of beer products can be ensured, clear and quantitative delivery access conditions of suppliers can be set according to the detection results, the risk of beer quality deterioration caused by quality problems of packaging materials can be avoided from the source, early prediction and precise control of the quality of packaging materials are realized, and the overall quality management level is improved.

The application provides a kit and method for detecting pig, chicken and duck derived components in meat or meat products based on CRISPR-Cas12a technology, wherein the kit comprises a LAMP primer combination, a Cas12a protein, crRNA and a fluorescence-quenching labeled single-stranded DNA reporter molecule. The identification method developed based on LAMP combined with CRISPR-Cas12a has the advantages of high specificity, high sensitivity and low equipment requirement, and is very suitable for on-site rapid detection.