4results about How to "Sensitive detection method" patented technology

The invention relates to the technical field of biological detection, in particular to a UXS1 enzyme activity determination method. The method comprises the following steps: a) hydrolyzing a substrate UDP-GlcA by using UXS1 to generate UDP-xyl; b) adding XYLT1 to catalyze the UDP-xyl to generate UDP (User Datagram Protocol); c) converting the UDP into ATP (adenosine triphosphate); and d) quantitatively detecting ATP, and deducing the enzyme activity of UXS1 through the obtained signal intensity. The method can effectively reduce the cost of UXS1 enzyme activity detection, can help a pharmaceutical enterprise to quickly screen drugs of related targets at high throughput, and is beneficial to promoting the research and development process of related drugs.

The present invention provides a method for determining the arsenic content in natural water using a headspace single drop microextraction-spectroscopic pipette method, which belongs to the field of detection technology. The equipment used in the detection method of the present invention has low cost, good portability, and is easy to operate. The sampling strategy can be adjusted in time to meet the needs of any specific environment. ‑1 The detection method of the present invention is sensitive, robust, reliable, simple and practical, and can quickly measure the nanomolar concentration of different forms of arsenic in natural water bodies in the field. The linear detection range of the detection method of the present invention for arsenic is 0.3-65nM (R 2 =0.9980), the detection limit is 0.1nM, the RSDs for 25nM As(III) measurement are <2.0% (n=10), and the recovery rate of As at different concentration levels (5, 10 and 20nM) in different samples (rivers, lakes, ponds and reservoirs) is 91.7%-97.4%. The method provided by the present invention is very suitable for field analysis of the content of various forms of arsenic in natural water bodies, and has broad application prospects.

The application discloses a method for quality prediction of tinplate cans based on iron ion migration amount, which comprises the following steps: sealing and soaking the tinplate cans filled with acetic acid, and performing temperature acceleration test; sampling on the third day of the temperature acceleration test, and detecting the iron ion migration amount; sampling on the tenth day of the temperature acceleration test, and detecting the iron ion migration amount; and predicting the quality of the tinplate cans based on the iron ion migration amounts on the third day and the tenth day. The application takes the migration amount on the third day as a short-term risk indicator, and takes the migration amount on the tenth day as a key quality determination standard of long-term stability indicator. Based on the detection results of the two time points, the iron ion migration trend of the tinplate cans within a six-month shelf period can be reliably predicted, the sensory quality and stability of beer products can be ensured, clear and quantitative delivery access conditions of suppliers can be set according to the detection results, the risk of beer quality deterioration caused by quality problems of packaging materials can be avoided from the source, early prediction and precise control of the quality of packaging materials are realized, and the overall quality management level is improved.

The application provides a kit and method for detecting pig, chicken and duck derived components in meat or meat products based on CRISPR-Cas12a technology, wherein the kit comprises a LAMP primer combination, a Cas12a protein, crRNA and a fluorescence-quenching labeled single-stranded DNA reporter molecule. The identification method developed based on LAMP combined with CRISPR-Cas12a has the advantages of high specificity, high sensitivity and low equipment requirement, and is very suitable for on-site rapid detection.