206results about How to "Sensitive detection method" patented technology

An apparatus and method for remote NMR/MRI spectroscopy having an encoding coil with a sample chamber, a supply of signal carriers, preferably hyperpolarized xenon and a detector allowing the spatial and temporal separation of signal preparation and signal detection steps. This separation allows the physical conditions and methods of the encoding and detection steps to be optimized independently. The encoding of the carrier molecules may take place in a high or a low magnetic field and conventional NMR pulse sequences can be split between encoding and detection steps. In one embodiment, the detector is a high magnetic field NMR apparatus. In another embodiment, the detector is a superconducting quantum interference device. A further embodiment uses optical detection of Rb—Xe spin exchange. Another embodiment uses an optical magnetometer using non-linear Faraday rotation. Concentration of the signal carriers in the detector can greatly improve the signal to noise ratio.

The present invention discloses a para-gene for an exogenous insertion vector of transgenic maize transformation event MON88017 and application thereof, the sequence of which is shown in table 1. The para-gene for the exogenous insertion vector of transgenic maize transformation event MON88017 provided by the invention can be used for specifically detecting whether the transgenic maize and related products contain transformation event MON88017 and the content of the transformation event; and the detection method is sensitive, accurate, simple and reliable, and has broad market prospects. The para-gene analyzes and confirms the sources of different bases in the para-sequence of the exogenous insertion vector of transgenic maize transformation event MON88017 for the first time, and determines the binding sites of the exogenous insertion vector sequence and the maize genome sequence, thus contributing to scientific research.

The invention relates to a pathogenic bacterium liquid-based thin-layer smear detection kit and a detection method thereof. The detection kit comprises a preconditioning liquid, a liquefier, a diluent, a differential staining liquid and consumable items. The detection method of the detection kit comprises the following steps: adding the strongly alkaline preconditioning liquid and the liquefier into a detection sample to carry out a homogeneous treatment on the sample, adding the diluter, adopting a liquid-based centrifugal settling thin-layer preparation technology to obtain multiple uniformly-distributed pathogenic bacterium liquid-based thin-layer smears, carrying out differential staining, observing and calculating according the staining reaction, the morphology and the arrangement mode of the pathogenic bacteria, thus working out a detection result of multiple pathogenic bacteria in the sample. The invention establishes a fast and sensitive detection method based on the pathogenic bacterium liquid-based thin-layer smears, the whole process can be completed within one hour, and the detection method is simple, thereby achieving high clinical popularization values.

The invention relates to a primer for amplifying novel coronavirus and an application thereof. The primer comprises at least one oligonucleotide primer capable of recognizing a specific region on a novel coronavirus N gene, and the sequence range of the specific region on the novel coronavirus N gene comprises a region sequence of a novel coronavirus reference strain genome 28778-28971. By adopting the primer, novel coronavirus nucleic acid can be rapidly detected from RNA extracted from samples such as blood, saliva, sputum and air; the method has the advantages of simplicity, convenience, quickness and no need of training of large instruments and professionals, can be used as a primary screening and environment detection method for novel coronavirus infected suspected pneumonia cases, and can also be used as an important reference for clinical diagnosis.

The invention relates to a producing method of a streptomycin antibiotic molecular imprinting biosensor. The producing method comprises the following steps of: 1, pretreating a glassy carbon electrode; 2, modifying an electrode by a molecular self-assembled film, namely, immersing the treated electrode in a polymerization base solution, and after introducing pure N2 for 10min, scanning for 20 circles by cyclic voltammetry within a potential interval of 0-0.8V, wherein the scanning speed is 0.05V/s; and 3, eluting a template molecule, namely, modifying the electrode by adopting the step 2, and then eluting through 70wt% of alcohol solution to obtain the streptomycin antibiotic molecular imprinting biosensor. Based on a molecular imprinting technology, by using an electrochemical polymerization method and a cyclic voltammetry method, a molecular imprinting electrode is produced from streptomycin sulphate as a template molecule and o-phenylenediamine as a functional monomer, a rapid and sensitive streptomycin detection method is established, and the basis is provided for small, automatic and low-detection-limit electrochemical sensors.

The invention discloses a fluorescent quantitative PCR detection method of spring viraemia of carp virus, and belongs to the technical field of aquatic virus detection. According to the fluorescent quantitative PCR detection method of the spring viraemia of carp virus, a specific primer probe set used for detecting the spring viraemia of carp virus is arranged and composed of specific primer pairs aiming at the spring viraemia of carp virus and fluorescent probes. By means of the provided primers, good sensitivity and specificity for identifying the spring viraemia of carp virus are achieved. By means of the fluorescent quantitative PCR detection method of the spring viraemia of carp virus, hemorrhagic tissues infected by the spring viraemia of carp virus can be detected, asymptomatic fish carrying the spring viraemia of carp virus and cells infected by the spring viraemia of carp virus can be detected, and great application prospects for rapid detection and supervision of the epidemic disease on entry-exit aquatic animals are achieved.

An unmarked aptamer fluorescence sensor for detecting tetracycline is characterized in that tetracycline is detected through the combination of the special recognition effect of thiazole orange on a G-quadruplex structure aptamer and the specificity between the aptamer and a target object through the following steps of detecting the change of the structure of the aptamer not combined with the target object and the structure of the aptamer combined with the target object through a circular dichroism spectrum, observing the system reaction of tetracycline, the aptamer and thiazole orange by detecting a fluorescence spectrogram, establishing the unmarked aptamer fluorescence sensor based on fluorescent probe thiazole orange for detecting tetracycline, detecting the selectivity of the established unmarked aptamer fluorescence sensor for tetracycline by detecting the fluorescence spectrogram, and detecting tetracycline in an actual sample by means of the established unmarked aptamer fluorescence sensor. A simple, rapid and sensitive detecting method with high selectivity for tetracycline is provided, has good practical value and is convenient to popularize.

The invention discloses an enzyme-linked immunosorbent assay method for detecting cadmium-metallothionein in the flesh of a shell. The method comprises the following steps of: inducing the shell to synthesize the cadmium-metallothionein by using cadmium chloride, inducing a rabbit to synthesize an antibody by using purified metallothionein, separating and purifying the antibody, using an electrophoretically pure rabbit anti-shell metallothionein antibody as a primary antibody and a goat anti-rabbit enzyme labelled antibody as a secondary antibody, and assaying the cadmium-metallothionein content of the shell by using the indirect competitive enzyme-linked immunosorbent assay method. The method has the advantages of scientific and rational principle and relatively mature technical process and is used for assaying the cadmium-metallothionein content of the flesh of the shell so as to indirectly reflect the cadmium pollution of the flesh of the shell and provide a fast and sensitive detection method for assaying the heavy metal cadmium in the flesh of the shell.

The invention relates to a detecting method for an eighteen-component codonopsis pilosula preparation, which comprises one or more of the following identification and/or detection methods: identifying the morphological characteristics of medical materials such as Tibetan codonopsis pilosula, Bangna (a traditional Chinese medicament), cassia seed, Tibetan drug sweetflag rhizome, Chinese tinospora stem, denucleated medicament terminalia fruit, gymnadenia conopsea, fructus terminaliae billericae, frankincense, Kukuinabu (a traditional Chinese medicament) and Baxiaga (a Traditional Tibetan medicament containing vasicine) in the preparation through a microscope; qualitatively identifying costustoot, the Tibetan drug sweetflag rhizome and the frankincense in the preparation through thin layer chromatography; and detecting the content of aconitine in the preparation. By the method, the eighteen-component codonopsis pilosula preparation can be effectively detected.

A chemical reagent box applies enzyme linked immunosorbent assay method to detect diethylstilbestrol. The cony IgG sensitizer is enveloped on the enzyme scale plate and the two are reacted through hatching, washnig, sample diluent and enzyme scale antigen adding after diethyl stilbestrol sensitizer is added. The detection is finished by washing, adding enzyme-substrate material to display coloure and using enzyme scale meter to detect A 450 nm.

The invention discloses a loop-mediated isothermal amplification (LAMP) detection method and a kit of soft-shelled turtle iridovirus (STIV). An outer primer comprises sequences shown as Seq ID No.1 and Seq ID No.2. A quick, sensitive and specific detection method for detecting the STIV is provided, field instant detection of viruses can be performed, and technical support is provided for healthy cultivation of soft-shelled turtles and the development of international trade.

The invention discloses a kit for predicting pancreatic cancer patient prognosis adverse risks and an application thereof; CXCL12 and CXCR7 expression degrees in a tissue specimen of a pancreatic cancer patient are determined through an immunohistochemical method, CXCL12 and CXCR7 as markers can rapidly, safely, conveniently and effectively predict the pancreatic cancer patient prognosis adverse risks in in-vitro assessment of pancreatic cancer prognosis, and the basis is provided for clinical diagnosis and treatment.

The invention discloses a reagent for detecting helicobacter pylori infection in gastric juice and a detection method thereof and belongs to the technical field of enzyme analysis. According to the prepared reagent, viscous materials in the gastric juice are decomposed into a soluble liquid by virtue of ambroxol hydrochloride, so that the helicobacter pylori in the gastric juice is dissociated and is added into a filter, the filter is taken down from a reaction device, free helicobacter pylori flows into a reaction hole along with a pretreatment fluid, a phenol reagent and an alkaline sodium hypochlorite solution are added, the helicobacter pylori has a urease strengthening effect, the urea is decomposed to generate dimolecular ammonia and monomolecular carbon dioxide after the helicobacter pylori is reacted with urea on an acetate fiber membrane, the ammonia is reacted with phenol and hypochlorous acid in an alkaline medium so as to generate blue indophenol, and the content of the blue indophenol is in direct proportion to the content of the helicobacter pylori. The detection method disclosed by the invention is simple in pretreatment, rapid, sensitive, accurate and low in price, is suitable for detecting the helicobacter pylori in the gastric juice and can serve as an important check index of detection of the helicobacter pylori in the gastric juice.

The invention discloses a one-step droplet digital PCR method for quantitatively detecting the GII type norovirus in fruits and vegetables. According to the method, RT-ddPCR is utilized, a primer and a probe for RT-qPCR detection of the GII type norovirus are combined for use, reaction is carried out, and then the high-sensitivity rapid detection for the GII type norovirus is realized. The method is high in sensitivity and can achieve about 2copies/[mu] L, but the sensitivity of the conventional RT-qPCR is usually 10 copies/[mu] L; the amplification efficiency is high, the amplification efficiency is 95.4%, R2 is equal to 0.9973. Under low copy number, compared with RT-qPCR, the method provided by the invention can more effectively avoid the influences of inhibitors in fruits and vegetables, and the false negative generation is reduced. Therefore, the detection method provided by the invention is sensitive, accurate and visual, a novel detection method is provided for the government departments including the agriculture department and the inspection and quarantine bureau, and related detection mechanisms and enterprises, and guiding significance is also achieved.

The invention relates to an EV71 early diagnosis method and a reagent kit. Particularly, recombinant protein from EV71 viruses is used as antigen, antibodies (monoclonal antibodies and polyclonal antibodies) generated after the animal immunization by the recombinant protein is combined with enzyme linked immunization and other methods for detecting early anti-EV71 antibodies IgM or IgG in human serum samples or directly detecting EV71 pathogens in the serum samples.

The present invention relates to a SARS coronary virus related polypeptide and its uses. Concretely, the invention uses polypeptide from SARS virus N albumen synthesized by manpower as antigen and the antibody (single anti, multi-anti) generated after the polypeptide is used for immunity for animals, and combines enzyme linked immunity and other methods to detect the early anti-SARS antibody IgM or IgG in human blood serum sample, or directly detect the SARS pathogen in the blood serum sample.

The invention provides primers for detecting sugarcane rust and a detection method thereof. The nucleotide sequences of the primers are represented by PM1 and PM2 in a sequence table. The invention further provides the method for detecting sugarcane rust; according to the method, total DNA of a sample is used as a template, the above-mentioned primers are used for PCR amplification, and determination is carried out according to the position of specifically amplified segments after amplification is finished. The method provided in the invention can effectively overcome defects in conventional detection methods like field symptom observation and indoor microscope or electron microscope observation, can rapidly, accurately, specifically and sensitively detect sugarcane rust and can be used for large scale sugarcane rust PCR detection.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer used for detecting infectious haematopoietic necrosis virus and application thereof. The primer disclosed by the invention is a primer group, wherein the primer group consists of a primer 1, a primer 2, a primer 3, a primer 4, a primer 5 and a primer 6; or the primer group consists of the primer 1, the primer 2, the primer 3 and the primer 4; nucleotide sequences of the primer 1, the primer 2, the primer 3, the primer 4, the primer 5 and the primer 6 are respectively expressed as a sequence 1, a sequence 2, a sequence 3, a sequence 4, a sequence 5 and a sequence 6 in a sequence table. Due to the adoption of the primers, an IHNV RT-LAMP (Infectious Haematopoietic Necrosis Virus Reverse Transcription Loop-Mediated Isothermal Amplification) visual detection method is established by adding SYBR Green I fluorescent dye into a reaction solution; meanwhile, according to the method, the sensitivity is high, the specificity is good, the cost is low and field high-flux fast detection can be realized without depending on any special instrument equipment, so that an important significance isi provided for introduction of trout fries, breeding of fishes without special pathogen type and pollution-free cultivation of trout.

The present invention relates to ionic chromatography method of detecting glyoxalic acid, glycolic acid, glyoxal and oxalic acid. The detection includes the steps of: weighing sodium carbonate and sodium bicarbonate and dissolving with deionized water to compound eluting liquid; weighing sodium hydroxide and dissolving with deionized water to compound eluting liquid; weighing sodium hydroxide and dissolving with deionized water to compound eluting liquid; diluting the sample with deionized water to concentration for detection and ionic chromatography detection; comparing the ionic chromatography detection result of the sample with corresponding standard work curve to obtain the composition and contents of the sample. The said method can fast detecting the said components simultaneously in high selectivity and high sensitivity.

The invention discloses a method for detecting glutathione in a solution by using a phosphorescent quantum dot Mn: ZnS. According to the method, a deoxidant and an inducer are not needed in detection, and interference of background fluorescence and diffusion light can be avoided; and complex sample pretreatment is not needed in detection. The method is especially applicable to detection of glutathione in a biological fluid and a wine. The Mn: ZnS phosphorescent quantum dot prepared in the invention has good water-solubility and stability and shows good application prospects in detection of solutions or water samples.

The invention relates to a detection method for a traditional Chinese medicine composition capable of eliminating toxin and relieving sore throat and application thereof. According to the detection method in the invention, the content of luteoloside in Chinese lantern plant is selected as a detection index, and a proper gradient elution method is selected so as to allow optimal separation effect to be exerted on luteoloside and other chemical components in the traditional Chinese medicine composition, the content of luteoloside in the traditional Chinese medicine composition can be accurately determined; the detection method is accurate, sensitive, simple and has good stability and repeatability and an excellent HPLC chromatogram peak pattern, so the content of luteoloside in the traditional Chinese medicine composition can be accurately determined; moreover, a method for determining the content of luteoloside, a method for identifying arctium fruit, a method for identifying balloonflower roots and a method for identifying licorice roots are combined together for evaluating the quality of the traditional Chinese medicine composition capable of eliminating toxin and relieving sore throat, so the quality of the traditional Chinese medicine composition can be effectively controlled and the safety and stability in usage of the traditional Chinese medicine composition are improved.

The invention adopts oligonucleotide aptamers for identifying special tripeptide sequence KAI, GEL, DGI and LAS, extanding oligonucleotide aptamers by Structure-switch and phosphorylation decorating 5' end thereof, synthetising four connexons; connecting oligonucleotide aptamers combinated with target albumen through proximity dependent DNA Ligation technique as chain ringlike DNA; amplifying detecting signals via rolling-circle DNA duplicate method; the technique can detect target albumen sensitively, the least detecting concentration can get nM level; this method with flexibility can adjust according needed numbers of ligand; can detect albumen composite, provide sensitive detecting method for chip technique using oligonucleotide aptamers as ligand.

The invention discloses a method for immediate detection of telomerase activity based on gas pressure change caused by H2O2 (hydrogen peroxide) decomposition catalyzed by a platinum nanoparticle. The method comprises the steps: when active telomerase exists, a telomerase primer modified on the surface of a magnetic bead is bonded to telomerase, thus the telomerase primer is extended along a 3' end of the telomerase primer and a DNA single strand with TTAGGG repeats is formed; the long DNA single strand can hybridize with multiple cDNAs, and by modifying the surface of the platinum nanoparticle with the cDNA, the platinum nanoparticle is fixed to the surface of the magnetic bead; then the platinum nanoparticle is utilized to catalyze H2O2 decomposition to produce oxygen, and the gas pressure of a system changes; then a portable barometer is adopted for detecting a gas pressure value of the system, so that the immediate detection of the telomerase activity can be realized. The detection method disclosed by the invention is simple, rapid and sensitive, and can be used for detecting the expression level of telomerase activity in different tumor cells, and the detection method is of great significance in the diagnosis and treatment of cancers with telomerase as a target point.

The invention discloses a method for rapid detection of transgenic rice line TT51-1 and primers for use. The method belongs to the technical field of molecular biology, and relates to detection methods of transgenic products, the principle is as follows: using 6 specific primers and a DNA polymerase with chain displacement activity for amplification of a sample template DNA at 61 DEG C, amplified products can reach 109-1010 copies in a short period of time, result identification can be realized as follows: determining whether the DNA is amplified or not by direct observation of color change in a reaction tube by naked eyes, and the method has the characteristics of being high in efficiency, fast, simple in operation, high in specificity and the like, and provides a convenient means for the rapid detection of transgenic rice.

A nest polymerase chain reaction (PCR) detecting method of a transgenic crop cauliflower mosaic virus relates to the field of molecular biology. The method designs two specificity outer primers and adopts a CaMV35S promoter published by an announcement NO. 953-6-2007 of a ministry of agriculture to detect a primer serving as an inner primer according to a conserved sequence of a cauliflower mosaic virus CaMV35S promoter, and the group of primers can detect the cauliflower mosaic virus CaMV35S promoter in different transgenic crops and has the characteristic of a broad spectrum. The detecting method has the advantages of being efficient, sensitive, accurate and the like, can avoid a 'false negative' result, and can effectively solve the technical problem of detecting transgenic crops. The nest PCR detecting method can provide technical support for agricultural transgenic organism safety management directly, can conduct effective monitoring on research, production and sales of transgenic crop products, and has important meaning on guaranteeing health of people, environment safety and biodiversity.