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A microfluidic paper-based chip for detecting hepatitis C virus antibody and its preparation method

A technology of hepatitis C virus and paper-based chips, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve the problems of limiting the feasibility, harm, and large sample consumption of HCV diagnosis

Active Publication Date: 2015-11-25
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the HCV diagnostic techniques themselves (such as ELISA and RIBA tests) are expensive, take a long time to test (6-7 hours), and consume a large amount of sample (more than 20 microliters) [7]
These all limit the feasibility of HCV diagnosis in large-scale populations, affect the coverage of HCV diagnosis, and eventually lead to the widespread transmission and serious harm of HCV

Method used

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  • A microfluidic paper-based chip for detecting hepatitis C virus antibody and its preparation method
  • A microfluidic paper-based chip for detecting hepatitis C virus antibody and its preparation method
  • A microfluidic paper-based chip for detecting hepatitis C virus antibody and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1: Preparation method of microfluidic paper-based chip for detection of hepatitis C virus antibody

[0084] Step 1: Provide microfluidic substrate

[0085] The paper base is Amersham Protran NC0.45 (purchased from GE healthcare).

[0086] Step 2: Making Microfluidic Paper-Based Chips

[0087] Using a pair of intermeshing, sharp-edged metal dies (such as Figures 9A-9B shown), follow the Figure 1A Dimensions shown were used to fabricate microfluidic paper-based chips at room temperature by embossing.

[0088] Step 3: Add 0.5 μl of HCV antigen mixture (mixed by ab7978, ab68616 and ab43027, channels 2 and 3), HCV core antigen (purchased from abcam, ab7978, lane 4), HCVNS5 antigen (purchased from abcam, ab68616, lane 5), recombinant HCVNS4 antigen (purchased from abcam, ab43027, lane 6), anti-human IgG antibody (purchased from Sigma-Aldrich, SAB3701331, lane 7); BSA is added dropwise on the detection area of ​​channel 8 as a blank detection area; detection are...

Embodiment 2

[0091] Example 2. Assembly of the kit

[0092] Assemble into a kit according to the following composition:

[0093] Microfluidic chip prepared by the method described in Example 1; horseradish peroxidase-labeled secondary antibody, sample diluent, washing solution and substrate. According to needs, the kit can also be equipped with accompanying supplies such as instructions for use, packaging, and droppers.

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Abstract

The invention relates to a microfluidic paper base chip for detecting an antibody to hepatitis C virus as well as a preparation method of the chip. The microfluidic paper base chip provided by the invention comprises a plurality of micro detection channels, wherein each channel comprises an HCV core antigen, an HCV NS5 antigen, an HCV NS4 antigen, an HCVNS3 antigen as well as the combination thereof. Owing to the multi-channel detection capability, the microfluidic paper base chip can detect a plurality of immune projects synchronously; miniaturized screening and confirming testing are integrated on the microfluidic paper base chip, so that the complex HCV diagnosis process is simplified greatly, and the diagnosis efficiency is improved greatly.

Description

technical field [0001] The invention relates to the field of disease diagnosis, in particular to a microfluidic paper-based chip for detecting hepatitis C virus antibody, a preparation method thereof, and a detection kit sample. Background technique [0002] Hepatitis C is an infectious disease caused by the hepatitis C virus (HCV), which spreads globally and has great harm. HCV kills more people than AIDS each year in the United States and is the leading cause of cirrhosis, liver cancer, and the need for liver transplantation [1]. WHO estimates that at least 150 million people worldwide are infected with HCV [2], and in the next ten years, the harm of HCV to global health will continue to increase. The cumulative number of HCV patients in my country has exceeded 43 million. A major reason for the prevalence of HCV is the low diagnostic rate of HCV. Most people with hepatitis C are unaware of their infection. By the time HCV infection is discovered, patients are often at...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/576
CPCG01N33/54386G01N33/56983G01N33/5767G01N2333/186
Inventor 郑直慕轩
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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