47 results about "A antigen" patented technology

The invention discloses a kit for detecting the content of a serum amyloid protein A. The kit comprises a reagent R1, a reagent R2 and a serum amyloid protein A calibrator, wherein the reagent R1 comprises a first buffer solution, a first electrolyte, a surfactant, a first stabilizer, a high-molecular accelerant and a first preservative; the reagent R2 comprises a second buffer solution, anti-human serum amyloid protein A antibody coated latex particles, a second electrolyte, a second stabilizer and a second preservative; the serum amyloid protein A calibrator comprises a third buffer solution, a third stabilizer, a third preservative, an antioxidant and an anti-human serum amyloid protein A antigen. The invention further discloses an application of the kit for detecting the content of the serum amyloid protein A to the detection of the content of the serum amyloid protein A and a method for detecting the content of the serum amyloid protein A by adopting the kit for detecting the content of the serum amyloid protein A. By adopting the kit and the method disclosed by the invention, the content of the serum amyloid protein A can be detected easily and rapidly.

The invention discloses a microcystlin monoclonal antibody and the preparation method and the application thereof. The microcystlin monoclonal antibody is a microcystlin-LR monoclonal antibody, and the preparation method thereof is that: 1) an amino group is introduced to a seventh amino acid residue N-methyl dehydroalanine on microcystlin-LR, thus obtaining amino modified microcystlin-LR polypeptide; the amino modified microcystlin-LR polypeptide is coupled with carrier protein to obtain complete A antigen; 2) the immunity mouse of complete A antigen obtained in step 1) to get spleen cell of immunity mouse, which then is fused with myeloma cell of mouse to screen out positive hybrid cancer cell strain; the positive hybrid cancer cell strain is cultured or is injected in sygeneous mouse abdominal cavity to induce ascites to get the microcystlin-LR monoclonal antibody. The monoclonal antibody can be used for examining microcystlin-LR and immunity affinity purification thereof.

A composition for treating a subject is provided. The composition includes antigen specific dimeric secretory IgA and pentameric IgM therapeutic. A process for manufacturing a medicament for the treatment of C. difficile associated disease in a human is also provided that the modification of antigen specific dimeric secretory IgA and pentameric IgM with secretory component to form a antigen specific dimeric secretory IgA and pentameric secretory IgM therapeutic. The antigen specific dimeric secretory IgA and the pentameric secretory IgM therapeutic is then mixed with formulating agents to create a capsule, tablet, liquid or suppository dosing form. The therapeutic is amenable to enrobement directly through microencapsulation or the dosing form is coated with an enteric coating. A method of C. difficile treatment with the therapeutic is also provided that is amenable to supplementation with concurrent or prior antibiotic administration.

A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

The invention discloses senile dementia Ad-GFP gene vaccine and a preparation method theerof and is Ad-GFP vaccine of flexible foldable multivalence Beta- amyloid 1-15 (ABeta1-15) of secretion co-expression and genetic adjuvant, which a flexible connecting peptide is used for connecting multivalence. The invention selects foldable multivalence ABeta1-15 as immunogen, which avoids the space rigid structure of multiple copy ABetaB cell epitopes, also ensures that ABeta15 epitopes are more easily exposed, thus enhancing immunogenicity and also increasing immunogenic molecular weight and reducing the possibility of degradation; a antigen delivery system that takes adenovirus as a carrier is selected, which avoids the defects of high challenge and high cost of chemosynthesis.

The invention discloses a phenylethanolamine A hapten, a related artificial antigen and a monoclonal antibody; the invention simultaneously discloses preparation methods and applications of the phenylethanolamine A hapten, the related artificial antigen and the monoclonal antibody. The phenylethanolamine A hapten is the product shown in the formula 1, and the product shown in the formula 1 and a carrier protein can be connected to obtain a phenylethanolamine A antigen. The phenylethanolamine A antigen can be used for preparing a phenylethanolamine A specific antibody. The preparation methods have the advantages of simple and practicable operation, low cost and high yield of the hapten. The phenylethanolamine A artificial antigen can be used for producing the specific antibody aiming at the phenylethanolamine A by immune animals, and can be used for preparing an ELISA kit for detecting phenylethanolamine A residuals; the kit has the advantages of simple and rapid operation, large sample treatment amount, high sensitivity, good specificity, etc.

A method is provided for the purification of Clostridium difficile Toxin A antigen comprising reacting impure Toxin A with immobilized mono-specific polyclonal antibodies. The polyclonal antibodies are coupled to a hydrazide group containing matrix such as hydrazide activated agarose gel. The immobilized antibody is specific for Toxin A and will greatly purify Toxin A from a Toxin A containing solution. Antibodies raised to Toxin A purified according to the method are of higher activity than antibodies produced from prior art purified Toxin A.

The invention relates to a serum amyloid protein A (SAA) measuring kit, and a measuring method thereof, and belongs to the technical field of medicine biological detection. The kit comprises a reagent (1), a reagent (2), a calibration material, and a quality control material. The reagent (1) comprises Tris-HCl, sodium azide, and PEG-6000. The reagent (2) comprises Tris-HCl, emulsion particles with an embedded anti-human serum amyloid protein A antibody, sodium azide, octyl phenol polyoxyethylene, and methyl paraben. The calibration material comprises a serum amyloid protein A antigen, and sodium azide. The quality control material comprises a serum amyloid protein A antigen, and sodium azide. The provided serum amyloid protein A (SAA) measuring kit and measuring method thereof have the advantages that compared with the latex immunological turbidimetry technology, the analysis sensitivity is high, the results are accurate, the operation is simple, and the cost is proper, and thus the method is convenient for popularization.

The present invention relates to a kit for determining feline blood type, wherein the kit includes a mixture comprised of a first monoclonal antibody and a second monoclonal antibody, wherein both antibodies recognize feline blood group specific A antigens. The present invention also relates to a method for determining feline blood type, wherein the method utilizes two distinct monoclonal antibodies, which recognize feline blood group specific A antigens.

The invention relates to a predication and verification method of a serum amyloid protein A (SAA) antigen epitope and a preparation method of a monoclonal antibody. The predication and verification method comprises the following steps: predicating surface accessibility, an flexibility sequence region, a beta turn, antigenicity, hydrophilicity, a linear epitope and a long-fragment epitope of a serum amyloid protein A antigen to obtain a plurality of epitope sequences respectively; removing repeated epitopes in the plurality of epitope sequences and other sequences contained in other relativelylong epitopes to obtain a plurality of non-redundant predication epitopes; and verifying whether the plurality of non-redundant predication epitopes are identification epitopes of the antibody or notby adopting ELISA (Enzyme Linked Immunosorbent Assay). According to the invention, the problem that the predication accuracy of a single method is low is overcome; through experimental verification, the problem that the SAA antigen epitope is unknown is solved; and the monoclonal antibody is prepared by applying synthesized antigen epitope polypeptide, so that the problem that SAA is difficult topurify and obtain is overcome and the cost is reduced by one time or more.

The invention relates to an influenza virus and 2019 novel coronavirus antibody combined detection device and a preparation method thereof and belongs to the field of medical detection equipment. Thekit is prepared by adhering a nitrocellulose film, a glass fiber film, a sample pad, absorbent paper and other auxiliary materials, wherein the nitrocellulose film contains an influenza virus A antigen, an influenza virus B antigen, a novel coronavirus S antigen and a goat-anti-mouse IgG polyclonal antibody in a solid phase, and the glass fiber film absorbs a high-specificity anti-human IgM antibody. On the basis of ensuring complete release of the immune colloidal gold, the reaction sensitivity is effectively improved, under the same threshold value, the dosage of the immune colloidal gold can be reduced, cost is saved, the influenza virus A, the influenza virus B and novel coronavirus IgM antibodies in a specimen can be detected at the same time, and complexity of production operation isnot increased. The test paper is high in sensitivity, strong in specificity, simple and convenient to operate, time-saving and strong in practicability.

The invention belongs to the field of medical detection equipment, and provides a combined device for synchronously detecting IgM antibodies of influenza A and B viruses and chlamydia pneumoniae and mycoplasma pneumoniae. The device comprises a double-channel clamping shell, a test strip FluA & B and a test strip CP & MP which are arranged in the double-channel clamping shell in parallel; influenza virus A detection lines arranged on a nitrocellulose membrane of the test strip FluA & B at intervals are coated with high-specificity influenza virus A antigens, influenza virus B detection linesare coated with high-specificity influenza virus B antigens, and first quality control lines are coated with quality control line coating receptors; chlamydia pneumoniae detection lines (CP) arranged on a nitrocellulose membrane of the test strip CP & MP at intervals are coated with high-specificity chlamydia pneumoniae antigens, mycoplasma pneumoniae detection lines (MP) are coated with high-specificity mycoplasma pneumoniae antigens, and second quality control lines are coated with quality control line coating receptors.

The invention discloses an enzyme linked immuno kit for detecting residual phenylethanolamine A and an application method thereof and belongs to the technical field of enzyme-linked immunosorbent assay methods. The enzyme linked immuno kit comprises an elisa plate coated by a benzene ethanolamine A antigen, phenylethanolamine A standard substance working solution, phenylethanolamine A antibody working solution, phenylethanolamine A IgG-HRP working solution, substrate solution, stop solution, concentrated washing solution and concentrated sample diluent. The enzyme linked immuno kit adopts an indirect competition enzyme-linked immunosorbent assay (ELISA) method. Residual phenylethanolamine A on the standard substance or the sample to be detected and an antigen pre-coated on the elisa plate contend for the phenylethanolamine A antibodies together. The application method can be used for directly detecting the phenylethanolamine A in animal derived food, urine samples, animal tissues and serum samples. The enzyme linked immuno kit has the advantages of being convenient, fast, sensitive and the like, and is suitable for detection of large-volume samples. In addition, the sensitivity of the enzyme linked immuno kit is 0.1ng/mL.

The invention relates to monoclonal antibodies 2E11 and 3B4 of a foot and mouth disease virus type A. The kit comprises an effective amount of the monoclonal antibody 2E11, an effective amount of a foot and mouth disease virus type A antigen, an effective amount of an enzyme-labeled monoclonal antibody 3B4, a detection reagent for detecting the antigen-antibody reaction of the foot and mouth disease virus type A, and a support medium, wherein the monoclonal antibody 2E11 is coated on a support medium. The solid-phase competitive ELISA detection kit provided by the invention solves the problems of large batch-to-batch difference and poor stability of the existing liquid-phase blocking ELISA kit, the detection titer reaches 1: 128, and the sensitivity is high.

The present invention provides compositions and methods directed to incompatible blood group antigens. In particular, the present invention relates to anti-incompatible BG-A antibody molecules targeting a novel immune epitope in the incompatible BG-A antigen, such as the epitope bound by CRC-A1. The invention also relates to nucleic acids encoding such antibody molecules; to host cells expressing or capable of expressing such antibody molecules; to compositions comprising such antibody molecules or fragments thereof; and to uses of such antibody molecules or such compositions, in particular for therapeutic and detection purposes in the field of cancer diseases.

The invention provides a protein chip for detecting respiratory virus antigens as well as a kit and a preparation method thereof. The protein chip for detecting the respiratory virus antigens comprises a glass matrix vector and a quality control protein probe fixed in a protein quality comparison hole in the glass matrix vector; quality control protein comprises an adenovirus antigen, a respiratory syncytial virus antigen, influenza A and B antigens, a parainfluenza virus antigen, a SARS virus antigen, an H5N1 antigen and an H1N1-a antigen. Through adoption of the technical scheme, the diagnosis of simultaneous detection of eight types or more types of viral infection can be realized through one experiment, so that the efficiency in diagnosis of respiratory viral infectious diseases is improved, anti-bioterrorism screening is assisted, and a purpose of efficient, time-saving and high-flux diagnosis is realized.

Disclosed herein are maytansinoid drug linker derivatives which can be linked to a antigen binding unit (Abu), and maytansinoid drugs linked with an antigen binding unit (Drug-Linker-Antigen binding Unit: D-L-Abu), for targeted delivery to disease tissues. D-L-Abu, D-L-Abu derivatives, and methods relating to the use of such drug conjugates to treat antigen positive cells in cancers and immunological disorders are provided.