Serum amyloid a (saa) assay kit

A serum starch and protein technology, applied in the field of medical biological detection, can solve the problems of unfavorable clinical promotion, insufficient measurement sensitivity, cumbersome operation, etc., and achieve the effects of favorable clinical promotion, high specificity and accurate measurement results

Active Publication Date: 2017-09-15
河北艾驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few known kits for detecting serum amyloid A, and the method also has major defects, such as insufficient sensitivity, cumbersome operation, high cost, etc., which are not conducive to clinical promotion and use.

Method used

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  • Serum amyloid a (saa) assay kit
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  • Serum amyloid a (saa) assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of Anti-serum Amyloid A Antibody Latex Particles

[0035] 1) Prepare 50mM MES buffer solution with a pH value of 6.0;

[0036] 2) Dilute the latex microspheres with the above-mentioned MES buffer solution, the concentration is 1% (v / v);

[0037] 3) Add EDAC to the latex microsphere suspension at a concentration of 40 mg / ml, and incubate at room temperature for 40 minutes;

[0038] 4) Wash the latex microspheres with the same volume of MES buffer, then centrifuge at 13000g / min for 30 minutes, wash the latex microspheres again with the same volume of MES buffer, then centrifuge at 13000g / min for 30 minutes, and then dissolve the latex microspheres in MES buffer;

[0039] 5) Dissolving the rabbit anti-human serum amyloid A antibody in the above-mentioned MES buffer at a concentration of 1 mg / mL;

[0040] 6) Mixing the antibody solution and the latex microsphere suspension, stirring and reacting at 37°C for 3 hours;

[0041] 7) Add ethanolamine to...

Embodiment 2

[0043] Embodiment 2: the preparation method of kit

[0044] (1) Reagent 1: Tris 100mM, sodium azide 5g / L, PEG-6000 4g / L, pH value 8.1;

[0045] (2) Reagent 2: Tris 100mM, sodium azide 1g / L, octylphenol polyoxyethylene 1g / L, methylparaben 1g / L, anti-human serum amyloid A antibody latex particles 1g / L, pH Value is 6.0;

[0046] (3) Calibrator: level 1 contains serum amyloid A antigen 6.25mg / L, sodium azide 5g / L, level 2 contains serum amyloid A antigen 12.5mg / L, sodium azide 5g / L, level 3 Serum amyloid A antigen 25mg / L, sodium azide 5g / L, level 4 serum amyloid A antigen 50mg / L, sodium azide 5g / L;

[0047] (4) Quality control product: serum amyloid A antigen 10mg / L, sodium azide 5g / L.

Embodiment 3

[0048] Embodiment 3: detection method

[0049] The serum amyloid A assay kit described in this example uses an automatic biochemical analyzer with dual reagent functions, such as Hitachi 7180 automatic biochemical analyzer, etc., and uses the two-point endpoint method for determination. Put reagent 1 and reagent 2 on the reagent position of the instrument at a ratio of 1:1, and place purified water, calibrators and samples on the corresponding positions of the sample tray. The operation is as follows:

[0050] Table 1 Example 2 reagent detection method

[0051]

[0052] Calculation: SAA content (mg / L) = ΔA 测定 / ΔA 测定 ×C 标准

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Abstract

The invention relates to a serum amyloid protein A (SAA) measuring kit, and a measuring method thereof, and belongs to the technical field of medicine biological detection. The kit comprises a reagent (1), a reagent (2), a calibration material, and a quality control material. The reagent (1) comprises Tris-HCl, sodium azide, and PEG-6000. The reagent (2) comprises Tris-HCl, emulsion particles with an embedded anti-human serum amyloid protein A antibody, sodium azide, octyl phenol polyoxyethylene, and methyl paraben. The calibration material comprises a serum amyloid protein A antigen, and sodium azide. The quality control material comprises a serum amyloid protein A antigen, and sodium azide. The provided serum amyloid protein A (SAA) measuring kit and measuring method thereof have the advantages that compared with the latex immunological turbidimetry technology, the analysis sensitivity is high, the results are accurate, the operation is simple, and the cost is proper, and thus the method is convenient for popularization.

Description

technical field [0001] The invention belongs to the technical field of medical biological detection, and in particular relates to a serum amyloid A (SAA) assay kit and a use method thereof. Background technique [0002] Serum amyloid A (serum amyloid A, SAA) is an acute time-limited response protein, which belongs to the heterogeneous protein in the apolipoprotein family, with a relative molecular weight of about 12 000. In an acute time-limited reaction, stimulated by IL-1, IL-6 and TNF, SAA is synthesized in the liver by activated macrophages and fibroblasts, which can increase to 100-1000 times the initial concentration, but the half-life is extremely short. Short, only about 50 minutes. Serum amyloid A is associated with high-density lipoprotein (HDL), which regulates HDL metabolism during inflammation. A particularly important property of serum amyloid A is that its degradation products can be deposited in different organs as amyloid A (AA) fibrils, which is a serious...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/92G01N33/543G01N21/82
CPCG01N21/82G01N33/54313G01N33/92
Inventor 张伟林黄龙梅王鑫
Owner 河北艾驰生物科技有限公司
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