33results about How to "Conducive to clinical promotion and use" patented technology

The invention relates to a phosphatidylinositol proteoglycan GPC3 protein fragment, which has an amino acid sequence represented as the SEQ ID NO.1. The GPC3 protein fragment is strong in antibody specificity, can be used for preparing a polyclonal or monoclonal antibody being high in titer or preparing a GPC3 protein standard sample, and can be used in GPC3 protein detection, comprising detections of the content, the activity and the structure. The invention also relates to a monoclonal antibody hybridoma cell strain GPC3-1H5. A GPC3 antibody secreted from the GPC3-1H5 is high in yield and titer and is sensitive in reaction, so that the GPC3 antibody can improve sensitivity and accuracy of detection. The GPC3-1H5 can be widely applied in the detection of expression of the GPC3, can reduce the production cost of the GPC3 and is beneficial to clinical popularization and application of GPC3 detection.

The invention relates to a lipoprotein phospholipase A2 detection reagent and a preparation and usage method thereof. The detection reagent can be used to automatically determine a lipoprotein phospholipase A2 content on a fully-automatic biochemical analyzer. The lipoprotein phospholipase A2 content in a biological sample can be rapidly and accurately determined in a high-throughput mode. The reagent and the method have advantages of simple operation, high sensitivity, good specificity, an accurate result and the like. Lipoprotein phospholipase A2 detection cost is effectively reduced, and the reagent and the method are good for clinical wide popularization and usage.

The invention discloses an imatinib immunogen, derivative, synthesis method, specific antibody and detection reagent and preparation methods. The imatinib immunogen prepared by the invention is high in immunogenicity, can be induced to obtain a high-titer anti-imatinib specific antibody and does not crossly react with sixty-two common drugs; the imatinib detection reagent prepared by using the antibody can accurately and quickly determine the content of imatinib in samples. Compared with the existing detection reagent in the market, the detection reagent disclosed by the invention has the advantages of simplicity and convenience in operation, high sensitivity, high specificity, accurate result and the like, the imatinib detection cost can be effectively reduced and the large-scale clinical popularization and use are facilitated.

The invention discloses an estriol homogeneous phase enzyme immunoassay reagent as well as a preparation method and a detection method thereof and belongs to the technical field of immunoassay reagents. An estriol immunogen prepared by the invention is high in immunogenicity, can be induced to obtain a high-titer anti-estriol specific antibody and does not have any cross reaction with 62 common drugs. The estriol immunoassay reagent prepared by the antibody is capable of accurately and rapidly determining the estriol content in biological samples such as urea, blood and the like. Compared with the existing assay reagents in the market as well as detection methods and preparation methods thereof, the detection reagent disclosed by the invention has the advantages of simplicity and convenience in operation, high sensitivity, high specificity, accurate result and the like, is capable of effectively reducing the estriol detection cost and is favorable for large-scale clinical popularization and application.

The invention provides 5-fluorouracil derivatives, 5-fluorouracil immunogens, antibody for the immunogens, and a 5-fluorouracil detection kit. The 5-fluorouracil derivatives provided by the invention have a structure shown in a formula (I) as shown in the specification, wherein R is -(CH2)n-COO- and n is an integer between 1 and 20. The 5-fluorouracil derivatives with the structural formula are combined with a specific carrier, so that the prepared 5-fluorouracil immunogens have high immunogenicity; and the antibodies generated by an animal immunized with the immunogens have relatively high sensitivity and specificity, and have high specific combining ability with 5-fluorouracil. Through use of a homogeneous enzyme immune detection technology, high-flux and fast detection of 5-fluorouracil by an full automatic biochemical analyzer can be achieved; and the detection is convenient to operate, high in sensitivity, high in specificity, accurate in result and the like; the 5-fluorouracil detection cost can be effectively reduced; and clinical promotion and application are facilitated.

The invention relates to a serum amyloid protein A (SAA) measuring kit, and a measuring method thereof, and belongs to the technical field of medicine biological detection. The kit comprises a reagent (1), a reagent (2), a calibration material, and a quality control material. The reagent (1) comprises Tris-HCl, sodium azide, and PEG-6000. The reagent (2) comprises Tris-HCl, emulsion particles with an embedded anti-human serum amyloid protein A antibody, sodium azide, octyl phenol polyoxyethylene, and methyl paraben. The calibration material comprises a serum amyloid protein A antigen, and sodium azide. The quality control material comprises a serum amyloid protein A antigen, and sodium azide. The provided serum amyloid protein A (SAA) measuring kit and measuring method thereof have the advantages that compared with the latex immunological turbidimetry technology, the analysis sensitivity is high, the results are accurate, the operation is simple, and the cost is proper, and thus the method is convenient for popularization.

The invention relates to a myeloperoxidase detection reagent and a preparation and use method thereof. The automatic determination of the myeloperoxidase content on a full-automatic biochemical analyzer can be realized by applying the detection reagent, the myeloperoxidase content in a biological sample can be quickly and precisely determined in high flux, and the detection reagent has the advantages of being simple in operation, high in sensitivity, strong in specificity, and accurate in result. the myeloperoxidase detection cost can be effectively reduced, and the detection reagent is in favor of clinical and extensive popularization and use.

The invention provides a creatinine derivative, a creatinine immunogen and a specific antibody as well as a creatinine detection kit. The creatinine derivative has a structure represented by the formula (I) as shown in the description, wherein R is -(CH2)n-COO- and n is an integer from 1 to 20. The creatinine immunogen, which is obtained by linking the creatinine derivative of which H on the specific site is substituted by R and which has the above formula and a specific carrier, has high immunogenicity, the antibody produced by immunizing and inducing animals is high in specificity and has strong specific binding capability with the creatinine; the high-throughput and rapid detection of creatinine can be achieved by virtue of a homogeneous enzyme immunoassay technology on a full-automatic biochemistry analyzer, the method has the advantages of high sensitivity, strong specificity and accurate results and is simple and convenient to operate, the creatinine detection cost can be effectively decreased and the method is conductive to clinical popularization and application.

An exterior-applied elemene medicine with slow release and low irritation is proportionally prepared from elemene, organic solvent, medicinal matrix, and the additive chosen from hyaluronic acid, sodium hyaluronate gel, hyaluronidase, carbomer, and chitin. Its preparing process is also disclosed.

The invention discloses an oxcarbazepine derivative, an immunogen, an anti-oxcarbazepine specific antibody as well as a preparation method and application of the oxcarbazepine specific antibody. Firstly, a novel oxcarbazepine derivative and recombinant human serum albumin obtained through genetic engineering modification are coupled to prepare an oxcarbazepine artificial antigen, then the oxcarbazepine artificial antigen is used for immunizing an experimental animal to obtain an anti-oxcarbazepine specific antibody, and ELISA detection shows that the specific antibody is high in specificity and sensitivity and can be used for preparing the oxcarbazepine specific antibody. An interference experiment shows that the specific antibody does not have any cross reaction with 100 common drugs; the anti-oxcarbazepine specific antibody is applied to preparation of an oxcarbazepine detection reagent, the oxcarbazepine detection reagent comprises an oxcarbazepine average phase enzyme immunoassay reagent and an oxcarbazepine latex enhanced immunoturbidimetry detection reagent, and the detection reagent can realize high-flux and rapid detection of oxcarbazepine on a full-automatic biochemical analyzer.

The invention relates to a sensitive blood returning remaining needle, which comprises a steel needle, a flexible tube, a clockwise device, a soft sealing body and an outer casing body, wherein, a sensitive blood returning device is installed at the rear part of the outer casing body, the rear end of the flexible tube is inserted and installed at the front end of the outer casing body, the clockwise device ensures that the rear end of the flexible tube is tightly clamped and fixed with the inner wall of the outer casing body, the flexible sealing body is fixedly installed in the outer casing body, the steel needle penetrates the flexible sealing body, the front half section thereof is inserted and installed in the flexible tube, to expose the point of the steel needle, and the tail part of the steel needle is connected with the sensitive blood returning device; the sensitive blood returning device is composed of a gasbag external supporting part, a gasbag and a gasbag internal supporting part, the opening of the gasbag is supported by the gasbag internal supporting part, the gasbag and the gasbag internal supporting part are installed in the external supporting part together, and are tightly clamped and fixed. The sensitive blood returning remaining needle has the advantages that the blood returning condition can be judged quickly during the transfusion process by medical care personnel, to reduce the work strength, and relieve the pain of patients; the operation of the product is convenient, and is favorable for being promoted and used during the clinical work; the structure and the manufacturing process are simple, the cost is low, and the sensitive blood returning remaining needle has very wide market prospect.

The invention belongs to the technical field of biological detection, and particularly relates to a lamotrigine derivative, a preparation method thereof and application of the lamotrigine derivative in homogeneous enzyme immunoassay reagents. The structural formula of the lamotrigine derivative is shown as the following formula (I). The lamotrigine immunogen with high immunogenicity, a corresponding antibody, a lamotrigine enzyme-labeled conjugate and a lamotrigine homogeneous enzyme immunoassay reagent are obtained by using the compound shown as the formula (I). The lamotrigine immunogen is strong in specificity and high in immunogenicity; the prepared anti-lamotrigine antibody is strong in specificity and high in titer; the prepared homogeneous enzyme immunoassay reagent can rapidly andaccurately determine the content of lamotrigine in a biological sample, can simultaneously determine a plurality of samples on a full-automatic biochemical analyzer, realizes high-flux and rapid determination of lamotrigine, has high accuracy and strong specificity, and has greatly improved precision and detection efficiency compared with the prior art.

The invention discloses an olanzapine derivative, an immunogen, an anti-olanzapine specific antibody as well as a preparation method and application of the olanzapine specific antibody. Firstly, a novel olanzapine derivative and recombinant sheep serum albumin obtained through genetic engineering modification are coupled to prepare an olanzapine artificial antigen, then the olanzapine artificial antigen is used for immunizing an experimental animal to obtain an anti-olanzapine specific antibody, and ELISA detection shows that the specific antibody is high in specificity and sensitivity and can be used for preparing the olanzapine specific antibody. An interference experiment shows that the specific antibody does not have any cross reaction with 100 common drugs; the anti-olanzapine specific antibody is applied to preparation of olanzapine detection reagents, the olanzapine detection reagents comprise an olanzapine average phase enzyme immunoassay reagent and an olanzapine latex enhanced immunoturbidimetry detection reagent, and the detection reagents can realize high-flux and rapid detection of olanzapine on a full-automatic biochemical analyzer.

The invention relates to a vanillylmandelic acid homogeneous enzyme immunodetection reagent based on brominated derivatives and a preparation method thereof. The vanilla mandelic acid brominated derivative and the synthetic method are specifically and newly researched and designed, and the vanilla mandelic acid brominated derivative immunogen is prepared from the newly researched and developed vanilla mandelic acid brominated derivative. An anti-vanillylmandelic acid specific antibody prepared by immunizing an experimental animal with the vanillylmandelic acid brominated derivative immunogen has no cross reaction with 30 common hormones and hormone metabolites. The detection sensitivity of a vanillylmandelic acid homogeneous enzyme immunodetection reagent based on a brominated derivative prepared from the anti-vanillylmandelic acid specific antibody and a vanillylmandelic acid brominated derivative enzyme-labeled conjugate reaches ng/mL level, and the concentration of trace vanillylmandelic acid in a biological sample can be accurately detected; the detection accuracy, precision, sensitivity and specificity are obviously higher than those in the prior art.

This invention relates to a safe clean one-off injector, it includes injector coat, core bar set in the coat, rubber piston connected to forward end of the bar, and the pinhead set at forward end of the coat. A protecting jacket is muff coupled to the coat, the backward is a beaded edge extend outward, the jacket can slide on the coat. The pin can be destroyed after pulled into the jacket, so the safety of patient, medical care personnel and environment can be efficiently protected. Its using habit is same to the personnel using habit and operation is simple. It is propitious to clinical using and can be mass produced by using existing equipment.

The invention discloses an aripiprazole key group derivative, an immunogen, an anti-aripiprazole specific antibody and a preparation method and application thereof. Firstly, a novel aripiprazole key group derivative and recombinant human serum albumin obtained through genetic engineering modification are coupled to prepare an aripiprazole artificial antigen, then the aripiprazole artificial antigen is used for immunizing an experimental animal to obtain an anti-aripiprazole specific antibody, and ELISA detection shows that the specific antibody is high in specificity and sensitivity and can be used for preparing the aripiprazole specific antibody. An interference experiment shows that the specific antibody does not have any cross reaction with 100 common drugs; the anti-aripiprazole specific antibody is applied to preparation of aripiprazole detection reagents, the aripiprazole detection reagents comprise an aripiprazole homogeneous enzyme immunoassay reagent and an aripiprazole latex enhanced immunoturbidimetry detection reagent, and the detection reagents can realize high-flux and rapid detection of aripiprazole on a full-automatic biochemical analyzer.

The invention discloses a metanephrine derivative, an immunogen, an anti-metanephrine specific antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, coupling a novel metanephrine derivative with recombinant bovine serum albumin obtained by genetic engineering modification to prepare a metanephrine artificial antigen, and then immunizing an experimental animal with the metanephrine artificial antigen to obtain an anti-metanephrine specific antibody. ELISA detection shows that the specific antibody is strong in specificity and high in sensitivity, and an interference experiment shows that the specific antibody has no cross reaction with 32 common hormones; and the anti-metanephrine specific antibody is applied to preparation of metanephrine detection reagents, including a metanephrine homogeneous enzyme immunoassay reagent and a metanephrine latex enhanced turbidimetric immunoassay reagent. The detection reagent can realize high-throughput and rapid detection of metanephrine on a full-automatic biochemical analyzer.