Vanilla mandelic acid homogeneous enzyme immunodetection reagent based on brominated derivative and preparation method of vanillic mandelic acid homogeneous enzyme immunodetection reagent

Abstract

The invention relates to a vanillylmandelic acid homogeneous enzyme immunodetection reagent based on brominated derivatives and a preparation method thereof. The vanilla mandelic acid brominated derivative and the synthetic method are specifically and newly researched and designed, and the vanilla mandelic acid brominated derivative immunogen is prepared from the newly researched and developed vanilla mandelic acid brominated derivative. An anti-vanillylmandelic acid specific antibody prepared by immunizing an experimental animal with the vanillylmandelic acid brominated derivative immunogen has no cross reaction with 30 common hormones and hormone metabolites. The detection sensitivity of a vanillylmandelic acid homogeneous enzyme immunodetection reagent based on a brominated derivative prepared from the anti-vanillylmandelic acid specific antibody and a vanillylmandelic acid brominated derivative enzyme-labeled conjugate reaches ng / mL level, and the concentration of trace vanillylmandelic acid in a biological sample can be accurately detected; the detection accuracy, precision, sensitivity and specificity are obviously higher than those in the prior art.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a vanillylmandelic acid homogeneous enzyme immunoassay reagent based on brominated derivatives and a preparation method thereof. Background technique [0002] Vanillymandelic acid (Vanillymandelic Acid, VMA), its structural formula is shown in formula IV: [0003] [0004] Formula IV. [0005] Vanillylmandelic acid, also known as 3-methoxy-4-hydroxymandelic acid (DL-3-Methoxy-4-hydroxymandelic acid, MHMA) or vanillyl mandelic acid, is the adrenal medullary hormone catecholamine (CA) substances As the terminal metabolite, almost all CA is metabolized in the body, a small part of CA undergoes the action of monoamine oxidase to generate p-dihydroxymandelic acid, and most of CA is converted into 3-methoxyepinephrine under the action of CA-O-methyltransferase Or norepinephrine, which is eventually metabolized to 3-methoxy-4-hydroxymandelic acid. The metabolism of CA...

AI Technical Summary

Problems solved by technology

[0006] The classic VMA detection methods mainly include: visible light spectrophotometry, which is cumbersome to operate and has poor accuracy; gas chromatography, which has relatively high requirements for instruments and complicated operation; high-phase liquid phase-electrochemical method, which is easy to detect. Due to the interference of instrument quality and working environment, the sensitivity and specificity of the determination need to be further improved; the diazo method, this method is cumbersome to operate, has low reliability, and the recovery rate, repeatability, and stability need to be improved

None of the above determination methods can meet the needs of VMA clinical testing. At present, there is a lack of VMA detection reagents with good stability, high sensitivity and strong specificity in the market, especially fully automated detection reagents that can accurately measure trace VMA content in biological samples.

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