8 results about "Estriol" patented technology

The invention provides a liquid chromatography-mass spectrometry method for detecting estrogen and metabolites thereof in human excrement. The estrogen and the metabolites thereof comprise estrone E1, estradiol E2, estriol E3, 2-hydroxyestradiol 2-OHE1, 2-hydroxyestradiol 2-OHE2, 16-ketoestradiol 16-ketoE2, 16-cycloestriol 16-epiE3 and 17-cycloestriol 17-epiE3, and the estrogen and the metabolites thereof comprise estrone E1, estradiol E2, estriol E3, 2-hydroxyestradiol 2-OHE1, 2-hydroxyestradiol 2-OHE2, 16-ketoestradiol 16-epiE3 and 17-cycloestriol 17-epiE3. Comprising the following steps: detecting an excrement sample by adopting high performance liquid chromatography-tandem mass spectrometry, and constructing a calibration curve of each estrogen and metabolite thereof to obtain a standard curve regression equation; by detecting human excrement samples, risks and discomfort caused by invasive examination are avoided, the method is particularly suitable for chronic liver disease patients needing to be repeatedly monitored, the content of estrogen in excrement can be accurately detected, and more reliable indexes are provided for early diagnosis and illness monitoring of chronic liver diseases. The change of the content of the estrogen in the excrement is closely related to the change of the liver metabolism function, and the early detection of the chronic liver disease can be realized by detecting the estrogen in the excrement.

The invention relates to a composition for improving vaginal dryness and relaxation and a preparation method thereof, the composition comprises a fusion protein as shown in SEQ ID NO: 4, the fusion protein RGD-Collagen-FGF2 is formed by connecting an RGD element (SEQ ID NO: 1), a human III-type collagen active fragment (SEQ ID NO: 2) and an FGF2 active fragment (SEQ ID NO: 3) through a linker to form a'retention-repair-regeneration 'synergistic network effect: RGD realizes positioning retention, Collagen constructs an ECM (extracellular matrix) scaffold, and the fusion protein is used for improving vaginal dryness and relaxation. The FGF2 activates epithelial regeneration; in a vaginal dry mouse model, local administration of the fusion protein can significantly improve vaginal epithelial thickness, ECM water content and mucoprotein expression and reduce epithelial permeability, the effect is better than that of estriol positive drugs, mucosal atrophy is reversed from a pathological mechanism for the first time, and hormone dependence risks are avoided.

The invention belongs to the technical field of immunodetection, and particularly relates to a specific binding antibody of estradiol as well as a preparation method and application of the specific binding antibody. The present application provides a specific binding antibody against estradiol (E2). The binding protein adopts rational design guided by computational simulation, the complementary determining regions (CDRs) of the binding protein are accurately optimized, the binding protein can target an estradiol characteristic structural domain with high affinity, structural analogues such as oestrone, estriol and corticoid can be effectively distinguished, and the binding protein shows excellent specificity. When being applied to the development of an in-vitro diagnostic kit, the aptamer can realize high-sensitivity and high-specificity quantitative detection of estradiol in a clinical serum sample, and a reliable tool is provided for precise diagnosis and treatment of related diseases.

This invention relates to the field of water treatment technology and discloses an intermittent electrocatalytic treatment method for trace estrogen in reclaimed water. The method is carried out in a three-dimensional electrode reactor. Before formal treatment, a pre-treated water sample containing the estrogen to be removed is used to pre-saturate the particle electrode. Subsequently, reclaimed water containing one or more estrogenic pollutants is continuously introduced into the reactor, operating at a current density of 1.0–10.0 mA / cm², a hydraulic retention time of 5–20 min, and an aeration rate of 0–3.0 L / min. An intermittent power supply mode with alternating power on and off is employed, wherein the ratio of power on time to power off time is 1:1, and the power off time is 2–120 s. During the power off phase, the estrogen to be removed accumulates on the surface of the particle electrode. During the power on phase, the particle electrode repolarizes to form a micro-battery and undergoes electrochemical oxidation in conjunction with the anode, causing direct oxidation and / or indirect oxidation mediated by an electrogenerated oxidant, while the particle electrode achieves in-situ regeneration. The method is applicable to the treatment of trace amounts of estrogens in reclaimed water, preferably one or more of estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), and bisphenol A (BPA). This invention can reduce the artificially inflated impact of adsorption on the evaluation of treatment effectiveness, and reduce energy consumption while ensuring treatment efficiency.

Disclosed is a vaginal ring delivering estriol. The ring is suitable for use in the treatment of vaginal and vulva atrophy. It has a core-shell structure of ethylene vinyl acetate copolymers. Both the core and the shell are loaded with estriol, with the concentration of estriol in the core exceeding that in the shell. The core and shell have a different copolymer composition, with the vinyl acetate in the core being higher than that in the shell.

The application discloses a test paper for detecting oestrogen and a preparation method thereof, which comprises the following steps: (1) preparing an As2MoO6@NiS compound, and adsorbing a to-be-detected substance antibody, i.e., a first antibody, on the surface of the As2MoO6@NiS compound to obtain a photothermal signal probe; (2) assembling a test paper strip, and fixing a to-be-detected substance antigen and a second antibody on a detection area and a quality control area respectively; (3) mixing the photothermal signal probe with a to-be-detected sample liquid, and inserting a sample pad end of the test paper strip into the mixture to perform chromatography; and (4) detecting a photothermal signal: irradiating the detection area with a near-infrared LED, collecting temperature by using a thermal imaging or temperature measuring device, and performing quantitative analysis according to a linear relationship between the temperature of the detection area and the concentration of the to-be-detected substance of the sample. The detection mode is flexible, the linear range is wide, the sensitivity is high, and the specificity is good.

Provides herein are compositions for the treatment of chronic vulval or perineal pain, and of symptoms or conditions associated therewith, comprising an estrogen, optionally estriol, and a tricyclic antidepressant, optionally amitriptyline, formulated for topical, transdermal or transmucosal administration. Also provided are methods for the production of said compositions and to uses thereof in the treatment of chronic vulval or perineal pain, and of symptoms or conditions associated therewith.

The invention belongs to the technical field of steroid drug molecules, and relates to an estrone purification method, which comprises the following steps: adding 1, 4-diazabicyclo [2.2. 2] octane into a solution containing an estrone crude product and a first solvent to obtain a precipitate, dissolving the precipitate in a second solvent, adjusting to be alkaline, filtering, collecting filtrate, adjusting the pH value of the filtrate to be less than or equal to 3, and filtering to obtain the estrone purification product. And extracting with a third solvent to obtain an organic phase, cooling, crystallizing, carrying out suction filtration, and drying to obtain refined estrone. According to the method, the problem of removal of methylation rearrangement impurities generated in the existing preparation technology of the estrone is solved, the methylation rearrangement impurities can be purified from 0.7%-1.5% to less than or equal to 0.10% at a time, the purity of the finally obtained estrone is greater than or equal to 99.8%, and the yield is stable. The method disclosed by the invention is green and environment-friendly, low in cost, less in material loss, good in purification effect and stable in process, and has extremely high industrial application value.