28 results about "Enzyme immunoassays" patented technology

The invention relates to the field of biological medicine, and provides a monoclonal antibody for recognizing Profilin-1 and application of the monoclonal antibody. The antibody provided by the invention is selected from at least one of the following: (1) light chain CDR sequences as shown in SEQ ID NO: 5, 6 and 7, and heavy chain CDR sequences as shown in SEQ ID NO: 8, 9 and 10; (2) light chain CDR sequences as shown in SEQ ID NO: 11, 12 and 13, and heavy chain CDR sequences as shown in SEQ ID NO: 14, 15 and 16; or an amino acid sequence having at least 80% sequence identity compared with (1) or (2). In addition, the invention also discloses an enzyme immunoassay kit prepared by using the antibody, and the enzyme immunoassay kit has relatively high sensitivity and specificity.

The invention relates to the technical field of optics, and discloses a visualization method of a biological tissue three-dimensional full-information atlas, which comprises the following steps of: acquiring image data, electron microscope data, mechanical data, protein distribution, omics information and histological staining images of biological tissues by using various biological visualization technologies, and correspondingly processing the acquired data to obtain a three-dimensional full-information atlas of the biological tissues; finally, organization information fusion and digital display are achieved. According to the method, a cross-scale feature correlation algorithm framework is innovatively established, and multi-source heterogeneous data fusion is performed on medical image topological data (CT / MRI), ultrastructure scanning data (SEM / TEM), molecular positioning information (immunofluorescence, immunohistochemistry and enzyme immunoassay), omics maps (space transcriptome / proteome) and high-resolution tissue staining data. The data island dilemma caused by traditional single-mode analysis is broken through, and full-scale visualization from nanoscale molecular distribution to centimeter-level organ tissue structure-function relationship is realized for the first time.

The embodiment of the invention relates to a to-be-detected item typesetting method and device, electronic equipment and a storage medium, and the method comprises the steps: under the condition that a target set type is an enzyme immunoassay item set, according to preset enzyme immunoassay strategy information and item names corresponding to all to-be-detected items, setting the to-be-detected items according to the preset enzyme immunoassay strategy information; performing layout sorting on each experiment number corresponding to the plurality of items to be detected, and adding a fixed position before the first experiment number and / or after the last experiment number of each item to be detected, so as to generate a first layout file suitable for the microplate reader; under the condition that the target set type is a fixed electrophoresis type item set, according to preset electrophoresis typesetting information, item names corresponding to all the to-be-detected items and the number of experiment numbers contained in all the to-be-detected items, experiment numbers contained in all the to-be-detected items are distributed, and generating a second layout file suitable for the immunofixation electrophoresis apparatus. The processing efficiency of to-be-detected items can be improved, and the risk of manual operation errors is reduced.

The invention discloses an enzyme-free cleaning system, an automatic enzyme-free detection device and a reaction container cleaning method.The enzyme-free cleaning system comprises a flushing barrel and a plurality of cleaning barrels, a filling pump can be communicated with the flushing barrel or one of the cleaning barrels through a control valve set, and a cleaning head comprises a liquid injection needle and a liquid suction needle; the filling pump can further be in fluid communication with a liquid injection needle of the cleaning head, the cleaning head can be matched with an enzyme immunoassay reaction container cleaning position of the cleaning tank, when the filling pump is communicated with one cleaning barrel, the filling pump drives cleaning liquid to be injected into an enzyme immunoassay reaction container in the cleaning position through the liquid injection needle, and a liquid suction needle of the cleaning head can further be in fluid communication with a waste liquid collecting tank. The length of the liquid suction needle is larger than that of the liquid injection needle, the cleaning tank further comprises a suction opening, the suction opening can also be in fluid communication with the waste liquid collecting tank, the waste liquid collecting tank is further connected with a vacuum pump, the vacuum pump can suck the waste liquid collecting tank, the waste liquid collecting tank is in a negative pressure state, and the enzyme no-clean system remarkably improves the cleaning effect.

A method for determining the indication for the prevention of persistent AR in the form of problem-based learning in primary school children, consisting of determining the body mass index (BMI, kg / m 2 ) within the range of +1 SDS or more, taking into account the age and sex of the child and IL 1β using the enzyme immunoassay method; with IL 1β equal to or more than 0.029 pg / ml, a child with overweight / obesity is classified as being at high risk for the development of persistent AR, requiring prevention in the form of problem-based learning.

1. The name of the design product: enzyme label analyzer (DR-8000 series). 2. The use of the design product: a medical testing instrument for enzyme immunoassay. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The present invention is applicable to the field of detectors, and provides an enzyme immunoassay detector and an enzyme immunoassay method, the enzyme immunoassay detector comprises: a sample scanning device for scanning a to-be-detected sample to obtain sample information containing the sample number; a sampling needle is mounted on the sample adding device; the cup splicing device is used for splicing the reaction cups; the controller is in signal connection with the sample scanning device, the sample adding device and the cup splicing device and is used for controlling the cup splicing device to splice the reaction cups matched with the samples in number onto at least one to-be-detected plate; and controlling the sample adding device to perform sample adding on the reaction cups on the to-be-detected plate according to the sample information. The enzyme immunoassay detector can meet the detection requirements of different sample sizes, waste of reaction cups and coating reagents is avoided, cup splicing and sample filling can be automatically carried out according to scanned sample information, manual participation is not needed, and the enzyme immunoassay efficiency can be improved while the enzyme immunoassay cost is reduced.

Disclosed is an antibody which binds to a symmetrically dimethylated arginine analyte that can be used to detect a symmetrically dimethylated arginine analyte in a sample, such as in a homogeneous enzyme immunoassay method.

The utility model discloses an enzyme -free diagnosis reagent detection is placed with rack relates to enzyme -free diagnosis reagent technical field, and the both sides of base are equipped with clamping plate symmetry, and the both sides of clamping plate are equipped with the frame between corresponding, frame includes upper fixed plate, middle fixed plate and lower fixed plate, and upper fixed plate and middle fixed plate are equipped with the card hole of accommodating test tube respectively, and the upper portion of lower fixed plate is equipped with the card slot of being suitable for the bottom of test tube, and the upper portion of upper fixed plate is equipped with cover, and the top of upper fixed plate is equipped with a plurality of fixed column, and cover is equipped with the insertion hole of being suitable for a plurality of fixed column. The design of multilayer fixed plate cooperation card hole, card slot can carry out multidirectional steady limit from the upper portion, the middle and the bottom of test tube, effectively prevent test tube from toppling or falling in the detection process because of the collision, the shaking, and the falling; cover can be stably covered on upper fixed plate through the cooperation of fixed column and insertion hole, can block the dust, impurity of outside and fall into test tube, guarantee reagent not to be contaminated.

The utility model is applicable to the field of medical equipment, and provides an automatic cup splicing device of an enzyme immunoassay detector, which comprises a plate moving mechanism for conveying an elisa plate to a cup removing position, and a plurality of independent reaction cups are placed on the elisa plate; the bare board table is used for placing a bare board, and a plurality of cup positions for placing independent reaction cups are arranged on the bare board; the cup moving mechanism is used for transferring the independent reaction cups on the elisa plate at the cup removing position to the cup position; the automatic cup splicing device comprises a plate moving mechanism, a cup moving mechanism and a controller, the controller is in communication connection with the plate moving mechanism and the cup moving mechanism and controls the plate moving mechanism and the cup moving mechanism to automatically operate, independent reaction cups on an elisa plate can be transferred to cup positions of an empty plate through the automatic cup splicing device, the whole process is controlled by the controller to be automatically completed, manual participation is not needed, the cup splicing efficiency is greatly improved, and the production cost is reduced. And automation is realized.

The invention discloses application of SLC35E2B in treatment of obesity, and belongs to the technical field of biology. According to the invention, an animal model with obesity and / or related to obesity is constructed by knocking out a mouse animal model through the constructed SLC35E2B gene, changing the expression of the SLC35E2B gene in an animal body, and / or changing the level or activity of SLC35E2B protein; by means of molecular biology, enzyme immunoassay, immunohistochemical staining, oil red staining and the like, the application of the SLC35E2B gene in constructing an obesity model is researched.

The disclosure is related to methods of treating a subject with Alzheimer's Disease (AD) using anti-amyloid beta (Aβ) therapy. The patient may be treated based on the measurement and analysis of biomarker levels, such as plasma Aβ42 / 40 ratio, pTau181 and pTau217. The disclosure includes a systematic approach called CP Convert for converting biomarker measurements across different analytical platforms, enabling comparison of pTau217 data from LC-MS / MS, SIMOA, and chemiluminescent enzyme immunoassay platforms. Methods demonstrate that pTau217 has superior diagnostic accuracy compared to Aβ42 / 40 ratio and pTau181 for detecting brain amyloid positivity. Implementation of pTau217 prescreening can significantly reduce Aβ-PET testing, decreasing patient burden and clinical trial costs. The CP Convert methodology can be validated using independent cohorts, demonstrating robust cross-platform conversion for research applications.

The present invention discloses the use of SLC35E2B in treating obesity, belonging to the field of biotechnology. The present invention constructs an SLC35E2B knockout mouse model, alters SLC35E2B gene expression in the animal, and / or modifies the level or activity of the SLC35E2B protein to create an animal model of obesity and / or obesity-related diseases. The invention then utilizes molecular biology, enzyme immunoassay techniques, immunohistochemical staining, Oil Red staining, and other methods to study the use of the SLC35E2B gene in constructing obesity models.

The utility model discloses an incubation module and full -automatic enzyme free instrument, wherein the incubation module includes the incubator cover, the incubation module is equipped with the placement area for placing the incubator cover, the incubator cover includes first cover, second cover, counterweight spare and heating assembly, second cover with first cover is connected to and is enclosed and forms the accommodation cavity, the counterweight spare is located in the accommodation cavity, the heating assembly includes heating spare and conducting structure, heating spare is located in the accommodation cavity, conducting structure with heating spare is connected, and at least part of conducting structure stretches out the accommodation cavity outside, when the incubator cover is located in the placement area, conducting structure can with external circuit guide to with heating spare heating. It is designed to improve the incubation efficiency while reducing the production of condensate during the incubation process, also avoid the risk of incubator cover being corroded.

Disclosed is an antibody which binds to a symmetrically dimethylated arginine analyte that can be used to detect a symmetrically dimethylated arginine analyte in a sample, such as in a homogeneous enzyme immunoassay method.

The utility model discloses a novel intelligent blood collection bag, which relates to the technical field of medical instruments and particularly comprises a blood collection bag main body, and a blood collection bag is arranged in the blood collection bag main body. The top of the blood collection bag is connected with a connecting pipe, the tail end of the connecting pipe is connected with a first branch pipe and a second branch pipe through a three-way connector, the tail end of the first branch pipe is connected with a blood collection needle, and the tail end of the second branch pipe is connected with a bypass sample reserving bag. According to the utility model, the problem that the blood sample tube is left mistakenly and left is avoided, and for a primary screening reactive specimen, a blood bag catheter specimen does not need to be rechecked; meanwhile, due to the special reserved sample storage tube, later blood bag catheter centrifugation and heat sealing work can be omitted, and the sample quality requirements of later enzyme immunoassay and nucleic acid detection are completely met; due to the integrated blood sample tube, the risk that workers are punctured by using a sample collecting needle is avoided.

Disclosed herein are antibodies that specifically bind to buprenorphine and its metabolites norbuprenorphine, buprenorphine glucuronide and norbuprenorphine glucuronide analytes. Such antibodies can be used to detect a buprenorphine analyte in a sample, such as in a homogeneous enzyme immunoassay method. Hapten structures to elicit such antibodies and conjugates useful in immunoassays are also described.

Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (EIA) and polymerase chain reaction (PCR) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing EIA and PCR methods, thereby making the techniques of the present invention more reliable.

The invention relates to the technical field of in-vitro diagnostic reagent chemiluminescence, in particular to an estradiol chemiluminescence enzyme immunoassay enzyme-labeled diluent and a preparation method thereof.The preparation method comprises the steps that 80% of purified water is taken, an MES buffer solution and sodium chloride with the corresponding mass are weighed and added into the purified water, the mixture is adjusted to the preset pH value, and a mixed solution is obtained; adding zinc chloride, magnesium chloride, casein, glucan 500, TX405 and PC300 into the mixed solution, stirring and mixing, and fixing the volume to 100% by using purified water to obtain a fixed solution; and filtering the standing solution through a nylon filter membrane to obtain a diluent. By optimizing buffer salt components, protein, saccharides, a surfactant and the like in the enzyme-labeled diluent, the thermal stability, titer, linearity and precision of a reagent can be improved, the accelerated stability of an E2 enzyme-labeled antibody in a working solution can be improved by 95% or above by adding casein and glucan 500, and therefore the problem that an existing enzyme-labeled diluent is poor in stability is solved.

Disclosed herein are antibodies that specifically bind to a methotrexate analyte. Such antibodies can be used to detect a methotrexate analyte in a sample, such as in a homogeneous enzyme immunoassay method. Hapten structures to elicit such antibodies are also described. Conjugates useful in immunoassays that use such antibodies are also described.

The present invention relates to a method for analyzing cortisol using hair to measure stress in companion animals. More specifically, the present invention relates to a method for analyzing cortisol using hair of a companion animal, which performs a pretreatment process of sampling, washing, grinding, and extracting a hair sample, followed by cortisol analysis on a microplate reader using a cortisol enzyme immunoassay kit. The analysis method of the present invention enables simple and accurate assessment of stress levels in companion animals.

Methods and kits for determining the presence and / or amount of one or more analytes in a keratinized structure (e.g., hair) sample. Such methods can include combining in an aqueous medium: a conjugate comprising an enzyme and an analyte of interest, an antibody specific for the analyte of interest, and the keratinized structure sample, and then determining whether the analyte of interest is present or absent in the keratinized structure sample.

The invention discloses a homogeneous enzyme immunodetection system based on a click chemistry coupling technology as well as a preparation method and application of the homogeneous enzyme immunodetection system. A technology (including micromolecule-protein carrier coupling and micromolecule-labeled enzyme coupling) for coupling a micromolecule hapten and a macromolecular protein by utilizing a specific coupling reaction of an azido functional group and an alkynyl functional group in click chemistry. The coupling reaction does not need to use a special activator, the reaction can be carried out under a very mild condition, and no compound containing two special structures of azido and alkynyl exists in a human body, so that the specificity of a small-molecule hapten and macromolecular protein coupling structure can be remarkably improved, and the sensitivity of the coupling reaction is improved. And the specificity of the micromolecular specific antibody can also be obviously improved. The key technology of a homogeneous enzyme immunodetection platform is improved, and compared with a traditional homogeneous enzyme immunodetection platform, the homogeneous enzyme immunodetection system based on the click chemistry coupling technology has the advantages of being easy and convenient to prepare, rapid in reaction, safe, free of pollution, low in cost, excellent in reagent performance and the like; and large-scale production and application are facilitated.

The invention relates to the technical field of enzyme immunoassay, in particular to an enzyme immunoassay full-automatic sample adding real-time monitoring device which comprises an enzyme immunoassay workstation and a sample adding module. A plurality of groups of sample adding pipes which are arranged side by side are mounted at the bottom of the sample adding arm, and sample adding needle heads for sucking samples are mounted at the bottoms of the sample adding pipes; a liquid blowing mechanism is further arranged on the outer side of the sample adding pipe, a lifting mechanism is fixedly connected above the liquid blowing mechanism, and a guide mechanism is fixedly connected below the liquid blowing mechanism; when the sampling needle head injects liquid for the last time, blowing equipment is started, air enters the hollow gear disc through the L-shaped fixing pipe and the fixing ring and is discharged from an exhaust port in the bottom of the liquid blowing pipe, liquid blowing is carried out on the bottom of the sampling needle head, and residual liquid is assisted to drop to a detection hole; meanwhile, the driving motor is started to drive the driving gear to rotate, and the liquid blowing pipe is driven to rotate through meshing transmission of the driving gear, the driven gear and the hollow gear disc, so that multi-angle blowing of the bottom of the sampling needle head is realized, and sufficient dripping of residual liquid is ensured.

The present invention belongs to the field of enzyme-linked immunosorbent assay detection, and specifically relates to an enzyme immunoassay analyzer, which includes a housing. The inside of the housing is hollow. A second flip cover is rotatably connected to the front end of the housing. Two symmetrically arranged guide rails are fixedly connected to the inside of the housing. A moving plate is slidably connected between the two guide rails. Two electric wheels are fixedly connected to one end of the moving plate away from the second flip cover. A reagent kit is arranged on the top surface of the moving plate. A detection module is fixedly connected to the inner top end of the housing. A display screen is fixedly connected to the top of the housing. After the culture is completed, the moving plate and the reagent kit are driven by the guide rails and the electric wheels to move under the detection module to detect the absorbance value. Through this setting, the pretreatment process of the enzyme immunoassay process is all placed inside the housing, and the whole process is mechanically operated, which not only facilitates the overall analysis process, but also makes the data more accurate and reduces the errors generated by humans.

This specification discloses antibodies that specifically bind to methotrexate analyte. Such antibodies can be used to detect methotrexate analyte in samples in homogeneous enzyme immunoassays and the like. Hapten structures for inducing such antibodies are also described. Furthermore, conjugates useful for immunoassays using such antibodies are also described.

The present invention discloses an antibody for recognizing the carboxylation site of matrix Gla protein and its application, wherein the antibody comprises at least one of the following CDR sequences: CDR sequences of heavy chain variable region: SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; CDR sequences of light chain variable region: SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. In addition, the present invention also discloses an enzyme-immunoassay kit prepared by using the antibody, which has high sensitivity.

The invention discloses a homogeneous enzyme immunoassay system based on a click chemistry coupling technology as well as a preparation method and application of the homogeneous enzyme immunoassay system. A technology (including micromolecule-protein carrier coupling and micromolecule-labeled enzyme coupling) for coupling a micromolecule hapten and a macromolecular protein by utilizing a specific coupling reaction of an alkynyl functional group and an azido functional group in click chemistry. The coupling reaction does not need to use a special activator, the reaction can be carried out under a very mild condition, and any compound containing two special structures, namely alkynyl and azido, does not exist in a human body, so that the specificity of a micromolecule hapten and macromolecular protein coupling structure can be remarkably improved; and the specificity of the micromolecular specific antibody can also be obviously improved. The key technology of a homogeneous enzyme immunoassay platform is improved, and compared with a traditional homogeneous enzyme immunoassay platform, the homogeneous enzyme immunoassay system based on the click chemistry coupling technology has the advantages of being easy and convenient to prepare, rapid in reaction, safe, free of pollution, low in cost, excellent in reagent performance and the like; and large-scale production and application are facilitated.