The invention provides a detecting furanketone metabolite ELISA kit, which contains: an ELISA plate covered by the original coated, enzyme marker, furanketone metabolite specific antibody or furanketone metabolite derivative antibody, furanketone metabolite standard solution or furanketone metabolite derivative standard solution, the substrate color solution, the termination solution, the condensed washing solution, the condensed complex solution. The invention also provides a method to apply the above ELISA kit detecting furanketone metabolite, which includes steps: firstly, sample pre-treatment, and then using the kit for testing, and finally analyzing testing results. The invention is to provide the furanketone metabolite residues in the ELISA kit for detection of animal derived foods, such as chicken, pork, fish, shrimp, milk, honey, egg, and other samples, and the detection method is simple, low cost, high sensitivity, and it can monitor on the scene and suitable for screening large number of samples.