Enzyme linked immunosorbent reagent casing for detecting furantoin metabolite and uses thereof

Abstract

The invention provides a detecting furanketone metabolite ELISA kit, which contains: an ELISA plate covered by the original coated, enzyme marker, furanketone metabolite specific antibody or furanketone metabolite derivative antibody, furanketone metabolite standard solution or furanketone metabolite derivative standard solution, the substrate color solution, the termination solution, the condensed washing solution, the condensed complex solution. The invention also provides a method to apply the above ELISA kit detecting furanketone metabolite, which includes steps: firstly, sample pre-treatment, and then using the kit for testing, and finally analyzing testing results. The invention is to provide the furanketone metabolite residues in the ELISA kit for detection of animal derived foods, such as chicken, pork, fish, shrimp, milk, honey, egg, and other samples, and the detection method is simple, low cost, high sensitivity, and it can monitor on the scene and suitable for screening large number of samples.

Description

technical field

[0001] The invention relates to an enzyme-linked immunoimmunoassay kit for detecting residues of furaltadone metabolites in samples such as chicken, pork, fish and shrimp, milk, honey and eggs in animal-derived foods and a detection method using the kit . Background technique

[0002] Nitrofuran drugs have been widely used as growth-promoting additives for poultry, aquatic products and pigs because of their excellent antibacterial and pharmacokinetic properties. However, in the course of long-term experimental research, it was found that both nitrofuran drugs and metabolites can cause cancer and gene mutation in experimental animals, so these drugs are prohibited from being used in treatment and feed. Furaltadone was banned in 1995.

[0003] The most common methods used to detect furaltadone metabolites are LC-UV, LC-MS and LC-MS / MS, which are not suitable for on-site monitoring and screening of a large number of samples due to complex instruments and cumbe...

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