95 results about "Nitrofuran" patented technology

A detection method of a nitrofuran metabolites and chloramphenicol multiresidue in shrimp belongs to the technical field of aquatic products detection. The method is as below: hydrolyzing a sample with hydrochloric acid, subjecting nitrofuran metabolites to derivatization by using 2-nitrobenzaldehyde, adjusting the pH value to 6.5-7.5, adding acetonitrile, adding an extraction salt package; conducting liquid-liquid extraction of a target compound by using acetonitrile; purifying and concentrating a supernatant; then determining by a liquid chromatography-tandem mass spectrometer, and quantifying by an internal standard method. The method uses a novel sample extraction and purification mode for simultaneous analysis and determination of two prohibited drugs with the highest detection rate in shrimp, overcomes the limitations of separate determination of two drugs in the detection method of the prior art, improves the extraction efficiency of chloramphenicol in actual positive samples, greatly improves the work efficiency, shortens the work time and saves reagent consumption and labor costs. The method adopts the isotope internal standard method for quantification, the determination result is more accurate and reliable, has high sensitivity, and good reproducibility and quantitative accuracy.

The application provides a detection method of nitrofuran drug metabolites in a meat product. The detection method comprises the following steps: (1) pretreatment comprising putting a sample into a pretreatment liquid, oscillating, then carrying out centrifugal separation, removing the clear liquid, and thus obtaining a solid residue; and (2) solid residue treatment comprising adding a derivatization agent into a sample solid residue extract liquid, carrying out derivatization treatment under conditions of heating, acidity and inert gas protection, after the derivatization treatment is completed, cooling, adjusting the pH to neutral, then centrifuging, taking the supernatant, then allowing the supernatant to pass through a solid-phase extraction column, leaching with a leaching liquid, carrying out elution treatment with an eluant to obtain an eluate, concentrating and drying the eluate, re-dissolving with a re-dissolving liquid, and thus obtaining a sample solution. The detection method can be applied to various animal-derived samples, and can shorten the hydrolyzed derivatization process to 30-60 minutes and shorten the whole detection process to 60-120 minutes.

The invention relates to an immune nano gold test strip for quickly detecting four nitrofuran medicaments. The immune nano gold test strip is prepared by the following steps of: synthesizing general hapten, immunogen and captured antigen aiming at the four nitrofuran medicaments comprising nitrofurazone, furaltadone, nitrofurantoin and furazolidone by using 5-nitrofurfural as a raw material, immunizing a domestic rabbit serving as an experimental animal to acquire polyclonal IgG antibody, preparing gold labeled antibody by using nano gold and the polyclonal IgG antibody, coating the gold labeled antibody solution on a Glass33 glass cellulose membrane, namely a combined release pad, coating the captured antigen and goat anti-rabbit antibody on a detection line and a quality control line of an AE99 nitrocellulose membrane respectively, sequentially sticking the AE99 nitrocellulose membrane, the combined release pad, namely the Glass33 glass cellulose membrane, an absorption pad Cotton linters 2668 and a sample pad Glass33 on a polyvinyl chloride (PVC) back board, and finally, cutting the back board into 4*50mm test strips. The test strip can simultaneously and quickly detect the four medicaments comprising nitrofurazone, furaltadone, nitrofurantoin and furazolidone in feed; and the cost of the test strip is less than 5 yuan, so the test strip is suitable to be widely applied in feed detection or food animal detection.

The present invention discloses enzyme-linked immune kits for detecting nitrofurans metabolite residue including antigenic enzyme label plate coated with nitrofuran meatbolites, enzyme labeled antibody working fluid, standard solution, substrate solution, substrate buffer solution, reaction termination liquid, condense washing liquid and sample diluted concentrated liquid. The present invention also discloses using method for detecting by said kits including steps of pretreatment of sample, detecting with kits, result process and analysis and so on. The kits provided by present invention employ directly competing enzyme-linked immunoadsorption analysis to detect not only multiple nitrofurans metabolite residues at the same time, but also singleness nitrofurans metabolite residue, avoid many times detection of the same sample and reduce miss ratio, has merits of high sensitivity, good stability, less steps and low cost, and suit for screening of a large mount of samples, and has importance practical significance.

The invention relates to a method for preparing nifuratel. The method comprises the steps of (1) synthesizing 2-(methylmercapto-methyl)-oxacyclopropane through epoxy chloropropane and sodium methyl mercaptide; (2) generating reaction between hydrazine hydrate and the 2-(methylmercapto-methyl)-oxacyclopropane to synthesize 3-methylmercapto-2-hydroxy-propyl hydrazine; (3) generating reaction between diethyl carbonate and 3-methylmercapto-2-hydroxy-propyl hydrazine to prepare N-amino-5-methylmercapto-methyl-2-oxazolidinone; (4) hydrolyzing 5-nitrofuran formaldehyde diacetate ester under an acidic condition to prepare 5-nitro-2-furaldehyde; (5) generating reaction between the 5-nitro-2-furaldehyde and the N-amino-5-methylmercapto-methyl-2-oxazolidinone obtained in the step (3) to obtain the nifuratel, wherein in the step (1), 15-crown ether-5 is used as a catalyst, so that the conversion rate is high; no organic solvent is used during posttreatment of a product, and the posttreatment is simple.

The invention relates to a method for detecting a nitrofuran drug in eel. The method comprises the following steps: weighing 4 g of an eel sample, adding 20 ml of a hydrochloric acid solution with concentration being 0.2 mol/L, adding 1 ml of a derivating agent, uniformly shaking with hands, performing a water-bath reaction at the temperature of 60 DEG C for 1 h, taking the sample out and coolingthe sample to room temperature, using a NaOH solution for adjusting the pH value to 7.0-7.5, performing centrifugation for 10 min, taking a half of a supernatant, using 5 ml of methanol and 5 ml of distilled water, activating a solid-phase extraction column; separating the supernatant by a column, using 5 ml of distilled water for leaching, removing a leacheate, using 2 ml of methanol for elutinga solid-phase extraction column, collecting an eluate, drying the material with nitrogen at the temperature of 40 DEG C, using 1 ml of a furan solution for dissolving, filtering the material by a microfiltration membrane, and performing HPLC-MS-MS detection. The method has the advantages of high extraction efficiency, short derivating time, and high sensitivity, precision and accuracy.

The invention provides an anion receptor based on 5-nitrofuran formyl hydrazone and phenolic hydroxyl. The anion receptor is obtained by mixing 2, 6-dimethylacyl-4-chlorophenol and 5-(4-nitro)-phenyl-2-benzoyl diazanyl furan according to certain proportion, stirring and refluxing the mixture for 3 to 8 hours at a temperature of between 70 and 100 DEG C by using anhydrous ethanol as a solvent and p-toluene sulfonic acid as a catalyst, distilling out most of the solvent through reducing the pressure, performing suction filtration after cooling, and performing recrystalization on the solid product by using DMSO-EtOH. The anion receptor has color comparison recognizing ability on fluorion, acetic acid radical and phosphoric acid dihydrogen radical in a DMSO solution. The anion receptor is dissolved in DMF to be prepared into 0.5 to 2.0 percent of solution which is cooled to room temperature to be prepare into stable anion receptor organic gel used for performing colorimetric detection on the fluorion, and the acetic acid radical and phosphoric acid dihydrogen radical ions in the DMSO solution. The anion receptor has the advantages of convenience for carrying, convenient use, high detection sensitivity and the like.

The invention relates to a chip kit for the quantitative detection of veterinary drug residues, comprising a veterinary drug extraction reagent, a chip and a molecular hybridization reagent. The chip kit is characterized in that the chip is fixed with a specific antibody respectively aiming at clenobuterol hydrochloride, ractopamine, streptomycin, chloramphenicol, ciprofloxacin, nitrofuran, sulfametoxydiazine and tetracycline. The invention can simultaneously quantificationally detect eight veterinary drug residues contained in four samples, only needs four hours in the detecting process, automatically interprets a result through a system, provides an ideal mean for the fast quantitative detection of the veterinary drug residues contained in meats, poultry and dairy products and has good application prospect.

The invention relates to an ubiquitin E1 inhibitor and a preparation method thereof. Series compound Y-1-benzene pyrazole silane-3,5-diketone and relevant derivative (such as 4-(4-ethyoxyl-3-methoxybenzylidene)-1-benzene pyrazole silane-3,5-diketone, and the like) with different alternative groups are prepared by a liquid-phase organic synthesis method according to the structure of PRY-41(4(4-(5-nitro-furan group-methylene]-3,5-dioxo-pyrazolidine-1-radical)-ethyl benzoate). The ubiquitin E1 inhibitor can be used for inhibiting protein degradation mediated by ubiquitination and nondegradation of ubiquitination, and can kill transformed cells containing p53 in the process of blocking up the NFkB activity by the inhibitor, that is to say, the inhibitor possibly has the potential property of treating tumor and can be used as an important tool for researching ubiquitin proteinase system mechanism.

The invention discloses a synthetic method of 5-nitryl-4, 5-dihydrofuran derivates, comprising: taking one of Beta-nitrostyrene with a substituent on a benzene ring, Beta-nitrofuran ethylene with a substituent on a furan ring, Beta-nitrothiophene ethylene with a substituent on a thiophene ring, Beta-nitropyrrole ethylene with a substituent on a pyrrol ring and Beta-nitropyridine ethylene with a substituent on a pyridine ring as well as manganic acetyl acetonate (III) as reactants, and anhydrous alcohol as a reaction solvent, and preparing the 5-nitryl-4, 5-dihydrofuran derivates by the reaction at a temperature of between 30 and 70 DEG C. The raw materials used in the method are low-priced and can be obtained easily, and have a plurality of species, and the synthesized products can serve as potential molecules having the biological activity and key intermediates; and the method utilizes ethanol as a reaction medium, so as to reduce the pollution, shorten the reaction time, simplify the reaction operation and post treatment process, and reduce production costs. The method has advantages of good selectivity and high yield.

The invention provides a nitrofurans drug metabolite assay kit, which consists of a furazolidone metabolite test paper strip, a furaltadone metabolite test paper strip, a nitrofurazone metabolite test paper strip and a furantoin metabolite test paper strip. Each test paper strip consists of a sample pad, a colloidal gold pad, a nitrocellulose membrane, a sample adsorbing pad and a PVC (polyvinyl chloride) supporting plate, wherein the colloidal gold layer is a nitrofurans drug metabolite monoclonal antibody polyester film marked with colloidal gold, the nitrocellulose membrane is sequentially coated with nitrofurans drug metabolite coupled carrier protein as a test line (a T-line) and goat anti-mouse IgG antibody as a quality control line (a C-line). The nitrofurans drug metabolite assay kit is produced according to the nano colloidal gold technology, antigen-antibody specificity reactions and principles of immunity competitive inhibition reaction, and is used for testing whether samples contain nitrofurans drug metabolites or not.

The invention relates to a pretreatment method of nitrofuran metabolites and belongs to the technical field of detection of the nitrofuran metabolites. The method comprises the following steps: S1) cleaning a sample and stirring the sample to obtain a test sample; S2) adding hydrochloric acid and a derivatization reagent into the test sample, and carrying out ultrasonic treatment for 1-1.5 h under50-65 DEG C; S3) adjusting the PH to 7.4, adding cyclohexane, neutral alumina and a layering promoting agent, and carrying out refrigerated centrifugation after shaking to obtain a bottom solid phase, a middle water phase A and a top organic phase; S4) carrying out solid-phase extraction on the middle water phase A, and then, carrying out eluting through an eluent to obtain eluate B; and S5) carrying out nitrogen drying on the eluate B, and carrying out initial mobile phase volume determination. The method has the effect of shortening the pretreatment time of the nitrofuran metabolites.

The invention discloses a derivative of nitrofuran medicament metabolites as well as high performance liquid chromatography fluorescence detection. The method mainly comprises the following step that the derivative of the nitrofuran medicament metabolites is synthesized by matching sodium methylate, methyl alcohol and ethanol hydrazine according to certain dosage, and thus the liquid chromatography fluorescence detection is realized.

The invention relates to a detection method for nitrofuran antibiotics. The detection method is characterized by measuring the concentrations of to-be-detected nitrofuran antibiotics in to-be-detectedsamples by adoption of a fluorescence quenching method by taking a POSS fluorescent porous polymer as a fluorescence material. According to the detection method, the fluorescence quenching method isadopted to rapidly detect the nitrofuran antibiotics in the to-be-detected samples, so that the detection method has the advantages of being high in sensitivity, good in selectivity, less in sample size and simple in method, and has practical application value for the detection of the nitrofuran antibiotics.

The invention provides a method for detecting nitrofuran metabolites in animal-derived food through a liquid-phase chip. The method comprises the steps of coupling four microspheres with different numbers with artificial antigens for nitrofuran metabolites such as AHD, SEM, AOZ and AMOZ to obtain coupled microspheres; performing biotin reaction on four AHD, SEM, AOZ and AMOZ monoclonal antibodies and a BNHS (biotin-hydroxysuccinimide) solution to obtain a biotin antibody; performing reaction on the nitrofuran metabolites, the coupled microspheres, the biotin antibody and streptavidin-phycoerythrin (SA-PE) to obtain liquid to be detected; finally detecting a mean fluorescence intensity (MFI) value of the reaction liquid, and performing data analysis. The method disclosed by the invention can be used for simultaneously detecting the AHD, SEM, AOZ and AMOZ in the animal-derived food according to the specificity; furthermore, the whole process is simple to operate and is economical, practical, quick and accurate, and high-flux detection can be realized.

The invention discloses a preparation method of a nitrofuran antibiotic sensor, and belongs to the technical field of new nano functional materials and biosensing analysis technology. The method comprises the following steps: firstly preparing an array of cobalt-nickel bimetal layered hydroxide nanosheets on a disposable electrode, directly preparing a polydopamine film containing an electron mediator and a molecularly imprinted polymer using the nitrofuran antibiotic as a template molecule successively on the array of cobalt-nickel bimetal layered hydroxide nanosheets one after another by adopting an in-situ growth method and utilizing a large specific surface area and high active hydroxyl functional groups, and eluting the molecularly imprinted polymer of the template molecule after thetemplate molecule is eluted and the original position of the template molecule became a hole, thereby preparing a nitrofuran antibiotic sensor.

The invention discloses a fabrication method of a cobalt-nickel oxide-based nitrofuran antibiotics sensor, and belongs to the technical field of novel nanometer functional material and biological sensing analysis. A cobalt-nickel thermometal oxide nanosheet array is fabricated on a disposable throwable electrode, a polydopamine thin film and a molecular imprinting polymer are directly and sequentially fabricated on the cobalt-nickel thermometal oxide nanosheet array by an in-situ growth method according to large specific area, and the molecular imprinting polymer is coated with luminal in an in-situ way and takes nitrofuran antibiotics as a template molecule. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecular imprinting molecule of the template molecule is eluted. Therefore, the cobalt-nickel oxide-based nitrofuran antibiotics sensor is fabricated and completed.

The invention discloses an immunocolloidal gold rapid test product evaluation method for a drug residue in an aquatic product, and relates to an immunocolloidal gold test strip/card quality evaluation method used for the residue of forbidden medicines, such as chloramphenicol, malachite green and nitrofuran metabolites. Based on confirmation of an immunocolloidal gold test strip/card qualitative method by taking forbidden medicines, such as chloramphenicol, malachite green and nitrofuran metabolites, as tested objects, a technician in a test strip/card production enterprise verifies a field test in a laboratory and an expert group reads the verification results and judges the quality and performance of the test strip/card. Parameters including sensitivity and specificity, actual detection rate of a positive sample, stability, matrix applicability and test time are verified in the field test. The method performs necessary technical evaluation on the product in an objective accurate way, so that it is ensured that a purchaser can buy the product meeting the performance index requirement.

The invention discloses a method for detecting nitrofuran veterinary drug residues in gelatin. Through the method of adding enzymes, sample pretreatment is improved, and the method is suitable for the detection of nitrofuran residues in gelatin and intermediate samples in the production process of gelatin. According to the method for detecting the nitrofuran veterinary drug residues in gelatin, through the method of adding the enzymes, sample pretreatment is improved, the derivatization is more adequate and uniform, the measurement recovery rate and the accuracy are improved, the operation process is simpler and quicker, the sensitivity of the detection method can be significantly improved, the practicality is high, and support is provided for the prevention and control of the nitrofuran veterinary drug residues in gelatin and the production process of gelatin.

The invention discloses a furazolidone AOZ-D4 synthesis method, which comprises: 1) carrying out a reaction on deuterated acetic acid and bromine in the presence of TFAA to obtain deuterated bromoacetic acid; 2) carrying out a reaction on the deuterated bromoacetic acid and oxalyl chloride (or SOCl2) in DMF, adding benzyl alcohol, and continuously carrying out a reaction to obtain deuterated benzyl bromoacetate; 3) adding the deuterated benzyl bromoacetate into the THF solution of NaBD4, and carrying out a reaction to obtain deuterated bromoethanol; 4) dissolving hydrazine hydrate in dehydrated alcohol, adding NaOH, stirring, adding the deuterated bromoethanol, and carrying out a reaction to obtain deuterated 2-hydroxyethyl hydrazine; and 5) carrying out a reaction on the deuterated 2-hydroxyethyl hydrazine and dimethyl carbonate in the presence of an alkali to synthesize furazolidone AOZ-D4.