22 results about "Nitrofuran" patented technology

The invention provides a D-A type organic fluorescent small molecule, a preparation method and application of the D-A type organic fluorescent small molecule in detection of nitrofuran antibiotics, and belongs to the technical field of fluorescent sensing. The D-A type organic fluorescent micromolecule is composed of 2-(2-aminophenyl) benzothiazole and an electron-donating group, the excitation spectrum of the D-A type organic fluorescent micromolecule is obviously overlapped with the absorption spectrum of nitrofuran antibiotics, and fluorescence quenching response can be shown through the inner filter effect; the trace nitrofuran antibiotics can be detected in a liquid phase in an ultrafast, high-sensitivity, excellent-selectivity and strong-anti-interference manner. According to the invention, a quantitative evaluation system can be constructed in combination with a smart phone, the RGB value of the image of the liquid-phase fluorescence sensor is output in real time, and rapid, accurate and visual quantitative detection of nitrofuran antibiotics in a real food sample or a water source is realized. In addition, the filter paper fluorescence sensor has the advantages of being portable and easy to prepare, and nitrofuran antibiotics can be rapidly identified on site in an actual scene.

The invention discloses a method for detecting nitrofuran metabolites in macrobrachium rosenbergii based on UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) and application of the method, and belongs to the technical field of safety evaluation and detection of aquatic products. According to the method, a macrobrachium rosenbergii sample is pretreated by utilizing a QuECHERS method, nitrofuran metabolites are extracted, and the method comprises the steps of adding acidified acetonitrile for extraction, adding ammonium acetate and sodium chloride for water removal, adding a dispersed solid extraction column for reducing a matrix effect and the like. After the treatment by the QuECHERS method disclosed by the invention, the obtained nitrofuran metabolite does not need to be subjected to a derivatization procedure, and an HILIC hydrophilic chromatographic column can be directly used for carrying out liquid chromatography-mass spectrometry analysis in a positive ion mode so as to detect the nitrofuran metabolite such as semicarbazide and the like. The detection method provided by the invention has the advantages of high sensitivity, strong accuracy, wide applicability and the like, and compared with the traditional detection method, the reagent waste can be avoided, and a large amount of time can be saved.

The present application relates to the technical field of nitrofurans metabolite content determination, and particularly relates to a method for determining the residual amount of nitrofurans metabolite in propolis. The method is that the pretreated propolis sample is detected by liquid chromatography-tandem mass spectrometry after gradient elution of the mobile phase; the pretreatment is that the propolis sample is sequentially hydrolyzed by hydrochloric acid, purified by HLB solid phase extraction column, derivatized by o-nitrobenzaldehyde, and purified by ethyl acetate treatment. The present application purifies the propolis sample after hydrochloric acid hydrolysis before o-nitrobenzaldehyde derivatization, so that o-nitrobenzaldehyde fully reacts with the nitrofurans metabolite in propolis, and the residual amount of nitrofurans metabolite in propolis is accurately detected by combining the selection and gradient elution of the mobile phase.

Novel compounds and methods of killing or inhibiting the growth of bacteria.

Disclosed herein, are synergistic compositions comprising a nitroimidazole compound, a nitrofuran compound, or an anti-tumor agent; and a low minimum inhibitory concentration antibiotic, and methods of treating, preventing or inhibiting a facultative anaerobe infection by administering the compositions to a subject.

The invention discloses a chitosan composite filler purification and detection method for nitrofuran metabolites in aquatic products, and belongs to the technical field of physical and chemical detection of drug residues. According to the detection method, after a sample is subjected to hydrochloric acid hydrolysis, 2-nitrobenzaldehyde derivation, ethyl acetate extraction, chitosan composite filler purification and nitrogen blowing concentration redissolution, rapid separation is achieved by means of ultra-high performance liquid chromatography, determination is conducted by means of a triple quadrupole / composite linear ion trap mass spectrometer, quantitative analysis is conducted by means of an internal standard method, and the quantitative analysis result is obtained. And the residual quantity of the nitrofuran metabolites in the aquatic product can be rapidly and accurately measured. The method disclosed by the invention is economical, simple, convenient, efficient, high in sensitivity and good in repeatability, and can be used as a conventional detection method for residues of four nitrofuran metabolites in aquatic products.

The application provides a D-A type organic fluorescent small molecule, a preparation method and application thereof in detection of nitrofuran antibiotics, and belongs to the technical field of fluorescent sensing. The D-A type organic fluorescent small molecule is composed of 2-(2-aminophenyl) benzothiazole and an electron-donating group, the excitation spectrum of the D-A type organic fluorescent small molecule is obviously overlapped with the absorption spectrum of nitrofuran antibiotics, fluorescence quenching response can be exhibited through internal filter effect, trace nitrofuran antibiotics can be detected in a liquid phase in a superfast, high-sensitivity, excellent selectivity and strong anti-interference manner. The application can be combined with a smart phone to build a quantitative evaluation system, real-time output of RGB values of a liquid-phase fluorescent sensor image can be realized, and rapid, accurate and visual quantitative detection of nitrofuran antibiotics in real food samples or water sources can be realized. In addition, the filter paper fluorescent sensor has the advantages of portability and simple preparation, and can rapidly identify nitrofuran antibiotics on site in an actual scene.

The present application relates to a kind of construction method of electrochemical sensor based on nitrogen-doped carbon cobalt nickel diatomic catalyst and its application, the electrochemical sensor is the composite material after nitrogen-doped carbon cobalt nickel diatomic catalyst is mixed with film-forming material is made on the surface of glassy carbon electrode and is prepared.The electrochemical sensor provided by the present application has high stability and selectivity, double metal center synergistic effect, shows excellent catalytic performance, significantly improves electrochemical reaction activity, and improves the sensitivity of sensor.The electrochemical sensor is used to detect carbazochrome, and has high detection sensitivity and response speed, especially in trace detection and complex sample anti-interference aspect has advantage.The present application can be applied to food safety monitoring, such as rapid detection of carbazochrome residue in animal feed or meat, and can also be used for trace detection of nitrofurans in water or soil, and has wide application prospect.

The application discloses a kind of detection methods of nitrofurans metabolites in macrobrachium rosenbergii based on UPLC-MS / MS and application thereof, belong to aquatic product safety evaluation detection technical field.The application utilizes QuECHERS method to the pretreatment of macrobrachium rosenbergii sample, extracts nitrofurans metabolites, including adding acidified acetonitrile extraction, adding ammonium acetate, water removal and adding dispersed solid extraction column to reduce matrix effect and other steps.After the QuECHERS method of the application is handled, the obtained nitrofurans metabolites do not need to be subjected to derivatization procedure, can directly use HILIC hydrophilic chromatographic column, in positive ion mode liquid chromatography-mass spectrometry analysis, detect aminourea and other nitrofurans metabolites.The detection method provided by the application has the advantages of high sensitivity, strong accuracy, wide applicability, compared with traditional detection method, can avoid reagent waste, save a lot of time.

The application provides a Cd(II) coordination polymer, the chemical expression of the polymer is {[Cd2(bptc)(dpb) 1.5 (H2O)]·(H2O)·0.5(dpb)} n , wherein H4bptc is 2,3,3',4'-biphenyl tetracarboxylic acid organic ligand, dpb is 1,4-di(pyridin-4-yl)benzene (dpb) nitrogen-containing ligand, the crystal of the complex belongs to a triclinic system, the space group is Pī, the cell parameters are c=13.3406(3), α=110.165(1)°, β=107.476(1)°, γ=97.459(1)°, and the asymmetric unit is composed of 2 crystallographically independent Cd 2+ ions (Cd1, Cd2), 1 deprotonated bptc 4‑ ligand, 3 dpb ligands (dpbI, dpbII, dpbIII; occupancy: dpbI is 1, and dpbII and dpbIII are 0.5), 1 coordinated water molecule (O9) and 1 lattice water (O10). The Cd(II) coordination polymer provided by the application can be used for specific detection of nitrofuran antibiotics in irrigated water bodies.

The pretreatment method for detecting multiple veterinary drug residues in animal-derived samples of the present invention belongs to the technical field of drug residue determination. It solves the problem that multiple veterinary drug residues cannot be detected using the same pretreated animal-derived sample. The pretreatment method for detecting multiple veterinary drug residues in animal-derived samples includes the following steps: weighing the animal-derived sample to be detected, adding an internal standard solution and mixing it evenly with the animal-derived sample to be detected, then adding o-nitrobenzaldehyde solution and hydrochloric acid solution for derivatization, adding water-saturated n-hexane and a complex solution to obtain the test object, and finally testing. The pretreatment method of the present invention meets the demand that nitrofuran drug metabolites and amide alcohol drugs can be detected simultaneously through a single pretreatment process, avoids the process of multiple weighing, derivatization and extraction of animal-derived samples during the detection process, better meets the needs of large-scale detection of animal-derived samples, and improves detection efficiency.

The application provides a high-sensitivity detection method for multi-residues of veterinary drugs in pig urine, and belongs to the technical field of detection of veterinary drug residues. The application first performs enzymatic treatment on the sample to be detected, thereby improving the detection sensitivity of salbutamol and ractopamine; then performs acid hydrolysis and derivatization treatment on the sample to be detected, thereby improving the reversed-phase chromatographic retention performance and mass spectrometry ionization efficiency of nitrofurans in the compound to be detected, improving the sensitivity, improving the polarity of the compound to be detected, thereby improving the chromatographic separation effect, effectively separating the interfering substances in the matrix, improving the anti-interference ability of the detected substance, and indirectly improving the detection sensitivity; clenbuterol, metronidazole, dimetridazole, olaquindox, chlorpromazine and chloramphenicol are mainly in free state, and are not affected by enzymatic and acid hydrolysis and derivatization during detection, can be detected together with the above-mentioned compounds to be detected, and the mass spectrometry derivatization and sensitivity detection of multi-residues of cross-class veterinary drugs are realized.

The invention provides a nitrofuran metabolite magnetic surface molecularly imprinted polymer as well as a preparation method and application thereof, and relates to the technical field of molecularly imprinted polymers. The preparation method comprises the following steps: by virtue of a surface imprinting polymerization method, taking CS-Fe3O4-coated HNTs as a carrier, and taking AHD and AMOZ as dual-template molecules; mAA is a functional monomer; a deep eutectic solvent synthesized by choline chloride and p-coumaric acid is used as an auxiliary functional monomer, AIBN is used as an initiator, and EGDMA is used as a cross-linking agent; the magnetic surface molecularly imprinted polymer is prepared by using a magnetic solid-phase extraction technology as a template and acetonitrile as a pore-foaming agent, and is successfully applied to enrichment and detection of nitrofuran metabolites in an actual sample by combining a magnetic solid-phase extraction technology and high performance liquid chromatography. According to the method, trace detection of nitrofuran metabolites such as AOZ, AMOZ, AHD and SEM can be achieved, the detection limit is as low as 0.25-0.93 ng / mL, and the adding standard recovery rate is 80-95% RSDlt; 3.5%; the zebra fish can also be applied to water environment restoration, the survival rate of zebra fish is remarkably increased, and it is proved that the zebra fish has toxicology relieving and ecological restoration functions.

The present invention relates to the technical field of porous material preparation and organic pollutant detection technology in aqueous phase, and specifically relates to a fluorescent covalent organic framework material that can be used for antibiotic detection, a preparation method and application thereof, wherein the covalent organic framework material is a novel sql planar interlaced topological structure. The covalent organic framework material of the present invention has a rare three-dimensional framework structure formed by the interlocking of sql two-dimensional planes, and has fluorescent properties and high stability. It can efficiently detect nitrofuran antibiotics in water through an electron transfer mechanism and is reusable. The preparation method of the present invention synthesizes a novel fluorescent covalent organic framework by designing and synthesizing a covalent organic framework with a planar interlaced structure and fluorescent properties. The preparation method is easy to operate, green and environmentally friendly, has low cost, and is easy to industrialize. The covalent organic framework prepared by the present invention has high stability and, combined with a fluorescent sensing mechanism, can efficiently detect nitrofuran antibiotic pollutants in aqueous phase.

The invention provides a Cd (II) coordination polymer, the chemical expression of the polymer is {[Cd2 (bptc) (dpb) 1.5 (H2O)]. (H2O). 0.5 (dpb)} n, H4bptc is a 2, 3, 3 ', 4'-biphenyl tetracarboxylic acid organic ligand, dpb is a 1, 4-bis (pyridine-4-yl) benzene (dpb) nitrogen-containing ligand, the crystal of the complex belongs to a triclinic system, the space group is Pi, the cell parameters are as follows: # imgabs0 # c = 13.3406 (3), alpha = 110.165 (1) degrees, beta = 107.476 (1) degrees, gamma = 97.459 (1) degrees, and a coordinated water molecule is a coordinated water molecule. The asymmetric unit consists of two Cd < 2 + > ions (Cd1 and Cd2) which are independent in crystallography, one deprotonated bptc4-ligand, three dpb ligands (dpbI, dpbII and dpbIII; the occupancy rate of dpbI is 1, and the occupancy rate of dpbII and dpbIII is 0.5), one coordination water molecule (O9) and one crystal lattice water (O10). The Cd (II) coordination polymer provided by the invention can be used for specific detection of nitrofuran antibiotics in irrigation water.

The invention relates to a construction method and application of an electrochemical sensor based on a nitrogen-doped carbon-cobalt-nickel diatomic catalyst. The electrochemical sensor is prepared by modifying the surface of a glassy carbon electrode with a composite material formed by mixing the nitrogen-doped carbon-cobalt-nickel diatomic catalyst and a film-forming material. The electrochemical sensor provided by the invention has high stability and selectivity, has a bimetallic center synergistic effect, shows excellent catalytic performance, remarkably improves the electrochemical reaction activity, and improves the sensitivity of the sensor. The electrochemical sensor has high detection sensitivity and response speed when being used for detecting carbadox, and especially has advantages in trace detection and complex sample interference resistance. The method can be applied to food safety monitoring, such as rapid detection of carbadox residues in animal feed or meat, can also be used for trace detection of nitrofuran drugs in water or soil, and has a wide application prospect.

The application discloses a furan oxadiazole ketone compound and a preparation method and application thereof in detecting CO3 2‑ or PO4 3‑ . The furan oxadiazole ketone compound has a structural formula. The application further provides a preparation method of the furan oxadiazole ketone compound, which comprises the following steps: reacting 5-nitrofuran-2-carboxylic acid and oxalyl chloride to obtain 5-nitrofuran-2-carbonyl chloride, then acylating 2-hydrazinyl pyrimidine with 5-nitrofuran-2-carbonyl chloride to obtain 5-nitro-N'-(2-pyrimidinyl) furan-2-carbonyl hydrazine, and forming a ring of the 5-nitro-N'-(2-pyrimidinyl) furan-2-carbonyl hydrazine with triphosgene to obtain the furan oxadiazole ketone compound. The furan oxadiazole ketone compound provided by the application is an optical probe, which can be used as a fluorescent probe to detect CO3 2‑ or PO4 3‑ in environmental water bodies, and has strong anti-interference ability.

The invention belongs to the technical field of food processing, and discloses a method for removing livestock and poultry by-product nitrofuran metabolites. The method comprises the following steps: soaking the livestock and poultry by-products in light salt brine, then transferring the livestock and poultry by-products into pickle liquid with the total amount of lactic acid bacteria higher than 108-109 cfu / mL, carrying out staged variable-temperature soaking, and then carrying out two-stage spin-drying treatment. According to the method, the removal effect on the livestock and poultry by-product nitrofuran metabolite is remarkably improved, the removal rate reaches 100%, meanwhile, the quality of the livestock and poultry by-product is kept to the maximum extent, the pickle processing waste liquid is reused, and the cost is reduced.

This invention discloses a method for preparing 3-(4-nitrofuran-3-oxomethyl)-3-azidomethyloxetane. The method involves reacting 3,3-dibromomethyloxetane with sodium azide under mild conditions to obtain the intermediate 3-bromomethyl-3-azidomethyloxetane, which is then reacted with 3-nitro-4-hydroxyfuran in an inexpensive and environmentally friendly inorganic alkaline solvent system under heating in the presence of a catalytic amount of phase transfer catalyst to obtain the target product. The product of this invention possesses excellent detonation performance and desensitizing properties, while also functioning as a liquid phase carrier for cast explosives, a monomer plasticizer, and a single-element explosive. It can also be polymerized for use as a structural energetic material or an energetic binder, demonstrating considerable application potential.

The invention discloses a 4-(2-(methylamino) thiazole-5-yl) pyrimidine-2-amine derivative and application thereof, the structural formula of the 4-(2-(methylamino) thiazole-5-yl) pyrimidine-2-amine derivative is shown in the specification, in the formula, R1 is hydrogen or methyl; r2 is a substituted benzoyl group, a substituted phenylacetyl group, and a substituted or unsubstituted benzene propionyl group; and R3 is a substituted phenyl group, a 5-nitrofuran-2-yl group or a 1H-imidazole-2-yl group. As a novel CDK9 inhibitor, the ESCC inhibitor fills the blank of special medicines for ESCC; pharmaceutical salts and compositions can be prepared, and development of oral or injection preparations is facilitated; the compound is suitable for esophageal cancer single-drug treatment or is combined with other targeting / immune / chemotherapeutic drugs to improve the curative effect and reduce the drug resistance risk; meanwhile, other CDK9 related tumors such as lung cancer and breast cancer can be expanded.

The invention discloses polymer microspheres and application thereof in extraction and determination of nitrofuran metabolites in aquatic products, and belongs to the field of analysis. According to the invention, the mesoporous GMA-EGDMA polymer microspheres rich in cis-diol are synthesized and are used for boric acid affinity extraction; meanwhile, 4-fluoro-3-formylphenylboronic acid is used for derivatization of NFMs so as to carry out pretreatment on an aquatic product sample. The GMA-EGDMA polymer microsphere disclosed by the invention shows excellent reusability, and still keeps good performance after six times of adsorption-desorption cycles; the boric acid affinity extraction method does not need to use an extraction solvent, so that the pretreatment process is simplified, and a mild extraction environment is kept. The determination method provided by the invention provides an economic, simple, rapid and sensitive alternative scheme for monitoring NFMs residues in aquatic products.

This invention discloses a method for preparing 3-(4-nitrofurazan-3-oxomethyl)-3-azidomethyloxetane. Using 3-nitro-4-hydroxyfurazan and 3,3-dibromomethyloxetane as raw materials, the method involves heating in a solvent under alkaline conditions and with the aid of a phase transfer catalyst to obtain 3-(4-nitrofurazan-3-oxomethyl)-3-bromomethyloxetane through a one-step reaction. Subsequently, the product is reacted with an azide reagent at low temperature to obtain the target product, 3-(4-nitrofurazan-3-oxomethyl)-3-azidomethyloxetane. The product of this invention possesses excellent detonation performance and desensitizing properties, while also functioning as a liquid-phase carrier for cast explosives, a monomeric plasticizer, and a single-element explosive. It can also be polymerized for use as a structural energetic material or an energetic binder, demonstrating considerable application potential.