236 results about "Ractopamine" patented technology

The invention discloses a method for preparing an immuno biosensor for measuring ractopamine (RAC) in the technical field of chemical detection. Nanogold, a carbon nano tube having conjugated bonding coupled RAC-bovine serum albumin, and prussian blue are modified on a glassy carbon electrode sequentially to obtain the electrochemical immuno biosensor. By the method, the electrochemical immuno biosensor which has high sensitivity, high stability and can quickly test the ractopamine can be prepared.

The invention discloses a molecularly imprinted polymer and a preparation method and application thereof. The molecularly imprinted polymer of the invention is the molecularly imprinted polymer, which is prepared by using ractopamine as a template, polymerizing the template with a functional monomer, a cross-linking agent, a pore-forming agent and an initiator to form a block polymer, grinding the block polymer, sieving the obtained powder, removing the template by using acetate methoxide and methoxide and carrying out vacuum drying on the obtained product and has high compatibility and obvious selectivity for the ractopamine. The molecularly imprinted polymer of the invention has high compatibility and selectivity for the ractopamine and has wide application prospect in analysis of a sample pretreatment solid phase extraction material of the ractopamine in substrates such as a feed, animal tissue or environment water and the like.

The invention relates to a clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and a method for processing a detecting sample, and belongs to the technical field of detection of a beta-receptor stimulating agent, wherein a test strip is arranged inside a shell of the three joint inspection card, and the clenobuterol hydrochloride, salbutamol and paylean three joint inspection card is formed by the adhesion of a sample gasket, a colloidal gold membrane, a cellulose nitrate membrane and a water absorbing membrane to a bearing backboard in turn; the colloidal gold membrane is a glass fiber membrane of a colloidal gold marker containing a clenobuterol hydrochloride antibody, a salbutamol antibody and a paylean antibody; three detection strips are arranged on the cellulose nitrate membrane and contain clenobuterol hydrochloride protein conjugate, salbutamol protein conjugate and paylean protein conjugate respectively; and a quality control strip containing an anti-rabbit antibody or an anti-mouse antibody is arranged. The clenobuterol hydrochloride, salbutamol and paylean three joint inspection card has the advantages of simultaneously detecting the clenobuterol hydrochloride, the salbutamol and the paylean in urine or feed, animal tissue, meat and liver. The inspection card is easy to prepare and quick and convenient to use, saves the detection cost, and has accurate result.

The present invention discloses a method for enriching clenbuterol, ractopamine and salbutamol in a urine sample of breeding livestock, and relates to the field of analytical chemistry, particularly to a sample pretreatment method. The method comprises: carrying out a sample pretreatment, adding sulfonic group surface-modified polystyrene superparamagnetic nanometer beads or sub-micron beads to adsorb, carrying out elution, and collecting the eluent, wherein the elution solution can be directly used for liquid chromatography-mass spectrometry to quantitatively analyze contents of clenbuterol, ractopamine and salbutamol. The method has characteristics of simple operation process, high extraction recovery rate, less elution solvent use amount, and no requirement of rotary evaporation.

The present invention relates to a method for preparing haptens of formulae I and II that are useful in the preparation of immunogens, antibodies and conjugates, for use in competitive immunoassays for the detection of ractopamine, isoxsuprine and ritodrine. The haptens are prepared by reacting a phenylethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E: in which, in formula I, Z1 is a crosslinker and Z2 is H and, in formula II, Z1 is H and Z2 is a crosslinker.

The invention discloses a ractopamine-clenbuterol fluorescent microsphere detection test strip and a preparation method and an application. The test strip comprises a sample absorption pad, a combined pad, a chromatography membrane and a water absorption pad, which are adhered onto a soleplate and sequentially and closely connected with one another; the combined pad comprises fluorescent polystyrene microsphere labeled protein; the chromatography membrane is provided with two detection lines and one quality control line, the detection lines are close to the combined pad, and the quality control line is close to the water absorption pad; one detection line is coated with ractopamine protein conjugate, the other detection line is coated with clenbuterol protein conjugate, the quality control line is coated with protein conjugate which can be combined with proteins in the fluorescent polystyrene microsphere labeled protein and is not combined with the ractopamine and clenbuterol; and the fluorescent polystyrene microsphere labeled protein is ractopamine monoclonal antibody and a clenbuterol monoclonal antibody labeled by the fluorescent polystyrene microsphere. The test strip can simultaneously detect the ractopamine and the cleubuterol and is rapid, simple, convenient, flexible and accurate in detection.

The invention discloses a portable ractopamine molecular imprinted silk-screen printed electrochemical sensor, and belongs to the technical field of fast detection. 60-100 [mu]L of an electric conductance solution contains a to-be-detected sample is dropwise added to the surface of a ractopamine molecular imprinted silk-screen printed electrode, the standing adsorption time is 50-200 s, then the ractopamine molecular imprinted silk-screen printed electrode is horizontally inserted into a clamping slot of a portable electrochemical detector, a current peak value during scanning is recorded, calculation is performed according to a standard curve between the ractopamine concentration and the current peak value I([mu]A)=-14.944logC([mu]M)+0.2641 (R<2>=0.9923); and finally, the detected concentration of ractopamine in the sample is displayed on an LED screen. The sensor has the advantages of high precision, high stability and high repeatability.

The invention discloses an enzyme immune agent box for detecting lake drugs of animal foodstuff; it also provides a method for using the agent to detect the lake drugs residue. The agent box comprises: enzyme mark plate which coats lake drugs antigen or antibody, lake drugs mouse monoclonal antibody or polyclonal antibody working solution, enzyme mark antibody or enzyme mark lake drugs antigen, lake drugs standard solution, base material color developing solution, compression cleaning liquid, ending solution and compression twin solution. The invention also discloses a method for applying the detecting method, which comprises: first doing sample front process, then using the agent box to detect, at last analyzing the detected result.

The invention relates to a preparation method of a magnetic molecularly-imprinted polymer for separating and purifying ractopamine, aiming to solve the problem of leakage of magnetic ferroferric oxide in the processes of template elution and practical application of an existing magnetic ractopamine molecularly-imprinted polymer. The method comprises the steps of (1) preparing magnetic ferroferric oxide nano microspheres; (2) preparing an ATPS-modified ferroferric oxide material (ATPS-Fe3O4); (3) preparing a magnetic Fe3O4 functional monomer (MAC-ATPS-Fe3O4); (4) preparing a magnetic RAC molecularly-imprinted polymer (Fe3O4@MIPs). The prepared ractopamine magnetic molecularly-imprinted polymer disclosed by the invention is stable in performance, and can solve the problem of leakage of magnetic ferroferric oxide; the magnetic polymer has a relatively fast adsorption rate to ractopamine, meets the needs of rapid detection, has a rapid magnetic separation capacity, and is very suitable for field sample treatment.

The invention relates to a method for visual rapid detection of clenbuterol by adopting a nanogold probe. According to the method, contents of three main components, namely clenbuterol hydrochloride, ractopamine and salbutamol, are visually detected by specifically using melamine-modified nanogold. The method is mainly characterized in that the nanogold probe is formed by modifying melamine onto the surface of nanogold. The nanogold probe can specifically react with the clenbuterol hydrochloride, the ractopamine and the salbutamol through hydrogen bonds to cause polymerization of the nanogold and color change of a solution. According to a color change scope of the solution, whether the clenbuterol hydrochloride, the ractopamine and the salbutamol exist in the solution and the contents of the clenbuterol hydrochloride, the ractopamine and the salbutamol can be judged with the naked eye. The detection method is simple and practicable, is low in cost and high in sensitivity and is suitable for spot rapid detection of batch samples.

A method of promoting or improving the feed efficiency and the muscle-to-fat ratio in animals by administering to the animals a therapeutically effective amount of a mixture of RR-ractopamine and SR-ractopamine is disclosed. Manufacturing methods for making RR / SR-ractopamine are presented.

The present invention discloses enzyme-linked immune kits for detecting ractopamine including antigenic enzyme label plate coated with ractopamine, enzyme labeled ractopamine antibody working fluid, standard ractopamine solution, substrate solution, substrate buffer solution, reaction termination liquid, condense washing liquid and sample diluted concentrated liquid. The present invention also discloses using method for detecting ractopamine residue by said kits including steps of pretreatment of sample, detecting with kits, result process and analysis and so on. The kits provided by present invention employ directly competing enzyme-linked immunoadsorption analysis has merits of high sensitivity and good stability, simplify greatly the operation steps and reaction time to reduce error brought by complex operation, reduce cost and suit for screening of a large mount of samples, and has importance practical significance.

The invention provides a group of oligonucleotide aptamers Apt-1, Apt-2 and Apt-3 which are capable of simultaneously identifying clenbuterol hydrochloride and salbutamol, an oligonucleotide aptamer CLB-2 which is capable of identifying the clenbuterol hydrochloride with high specificity, an oligonucleotide aptamer SAL-5 which is capable of identifying the salbutamol with high specificity and two oligonucleotide aptamers RAC-5 and RAC-6 which are capable of identifying ractopamine with high specificity. Through an SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology based on Fe3O4 magnetic nanoparticle separation, a random oligonucleotide library is immobilized on avidin-enveloped magnetic nanoparticles by virtue of a complementary chain of a biotinylation marker, and the oligonucleotide aptamers, which are high in specific affinity, are finally obtained by conducting screening by 16 turns. The aptamers are broad in application prospect; and the aptamers, by virtue of marker function genes or fluorescent dyes, are applicable to detection of the clenbuterol hydrochloride, the salbutamol and the ractopamine in food; therefore, a new choice is provided for existing detection methods which depend on antibodies.

The invention relates to a novel synthesis method for a multilayer protection hyperstable water-soluble single fluorescent quantum dot and a fluorescent microsphere. The method comprises the following steps: transferring an oil-soluble fluorescent quantum dot to a water phase by using an amphoteric oligomer; then wrapping a water-soluble single fluorescent quantum dot and a fluorescent microsphere in silica; allowing the surfaces of the single fluorescent quantum dot and the fluorescent microsphere wrapped by silica to carry hydrophobic groups; and finally preparing the multilayer protection hyperstable water-soluble single fluorescent quantum dot and the fluorescent microsphere by using the amphoteric oligomer again. With the method, the fluorescent quantum dot can be protected from a destructive effect of an external environment (e.g., a strong acid, a strong alkali, a salt, etc.) to a greatest extent. The multilayer protection hyperstable water-soluble single fluorescent quantum dot and the fluorescent microsphere can be used in a microporous immunostrip biological detection system for detection of human chorionic gonadotropin (HCG), human immunodeficiency virus (HIV), hepatitis and clenbuterol--ractopamine(Rac) residual and the like in swine urine.

This invention uses immunity compatible chromatography post and enzyme linked immunosorbent assay to detect the remain RactopamineHy-drochloridie in food. It belongs to immunology and food safety detect technology. The method is: prepare muti-clone antibody by immunity antigen synthesis, antigen peridium, animal immunity and antigen depuration. Then put the antigen on to agarose gel to prepare immunity compatible chromatography post. You can enrich the RactopamineHy-drochloridie in the sample by compatible post and gather the eluent of the compatible post, then use linked immunosorbent assay to determine the consistency of the RactopamineHy-drochloridie.

The invention discloses a particle used for detection of a veterinary drug micromolecule. The particle comprises a receptor particle and a donor particle and is prepared through the following steps: marking carrier protein with a veterinary drug molecule; coupling the veterinary drug molecule-marked veterinary drug with a homogeneous chemiluminescent receptor ball; and preparing a homogeneous chemiluminescent receptor ball coupled with a veterinary drug molecule antibody. The invention further discloses a one-step homogeneous chemiluminescent method for detection of the micromolecule with the particle. The one-step homogeneous chemiluminescent method is used for homogeneous, rapid and high-sensitivity detection of veterinary drug residue and for testing of the contents of frequently used veterinary drug components in feeds, e.g., clenbuterol, ractopamine, salbutamol and terbutaline.

The invention relates to an immunity bead chromatograph test strip for quick testing ractopamine and a preparation method thereof, belonging to the technical field of ractopamine test. The invention is composed of a scale board, a sample pad, a magnetic combination pad, a coated film, an absorbent pad and the sample pad; the magnetic combination pad, the coated film and the absorbent pad are connected one by one on the scale board. The magnetic combination pad is made from absorbed glass mat which is used to absorb ractopamine magnetic antibodies; detection line T which is printed by the carrier protein solution coupled with ractopamine and control line C which is printed by goat anti-rabbit IgG are positioned on the coated film. The preparation method comprises the following steps: inserting the sample pad of the test paper in the sample solution to be tested, taking out the sample pad after 10-20 seconds, reacting at room temperature for 15 minutes, testing the test paper in a magnetic signal detector which outputs the biological response signals converted to magnetic field signals in the form of electrical signals; drawing standard curve and then evaluating the ractopamine content in the sample to be tested according to the standard curve. The invention applies the nano-magnetic immunity technology to test ractopamine and has the characteristics of high sensitivity, short reaction time, cheap apparatus, simple usage and the like.

The invention relates to a ractopamine aptamer, an electrochemical biosensor for detecting the ractopamine aptamer same, a method for manufacturing the electrochemical biosensor and a method for detecting the ractopamine aptamer by using the electrochemical biosensor. The ractopamine aptamer is a single-chain DNA (deoxyribonucleic acid) probe which contains an AGTGCGGGC sequence and has a length of 13-20bp, and the amino group of 5'-NH2-(CH2)6- is modified on the 5' terminal. The electrochemical biosensor for detecting the ractopamine aptamer comprises the ractopamine aptamer, has higher ractopamine detection sensitivity, can be used for detecting 0.1ng / mL of ractopamine at least, ensures that the ractopamine detection cost is lowered greatly, is easy and convenient to operate and can realize the rapid detection of the ractopamine.

The invention discloses a preparation method of ractopamine molecularly-imprinted materials, and is characterized by comprising the following steps: (1) the activation of silica gel: weighing silica gel, and carrying out backflow activation on the silica gel by using an acidic aqueous solution; (2) the double-bond bonding of the silica gel: after the activated silica gel and a double-bond containing silane coupling agent are heated, and carrying out a reaction on the two; (3) the surface chemical grafting of the silicon gel: putting the silica gel into a polyethylenimine aqueous solution to carry out a heating reaction to obtain an adsorbing material; and (4) product preparation: adding the adsorbing material into a ractopamine aqueous solution, adjusting the system pH value of the obtained mixture to an alkaline value, adding a crosslinking agent in the alkaline mixture, and carrying out a heating reaction on the alkaline mixture; and after the heating reaction is completed, washing off template molecule ractopamine, and drying the obtained object so as to obtain a ractopamine molecularly-imprinted material. Compared with the prior art, the preparation method disclosed by the invention has the advantages that the method is simple in preparation process, strong in maneuverability and low in preparation cost; and the prepared molecularly-imprinted material is good in specificity, short in adsorption equilibrium time to ractopamine, short in elution time and good in elution effect.

The invention relates to a ractopamine molecular-imprinting piezoelectric sensor and a preparation method thereof. The technical scheme is mainly technically characterized in that ractopamine is adopted as a template molecule, o-amino phenylthioalcohol is adopted as a functional monomer, an electropolymerization technology is adopted for synthesizing a poly-o-amino phenylthioalcohol film with high recognition selectivity to ractopamine on the surface of a quartz-crystal gold electrode self-assembled with gold nano particles, so that ractopamine can be quantitatively detected. The ractopamine molecular-imprinting piezoelectric sensor and the preparation method thereof have the beneficial effects that the characteristics such as high specific surface area of the gold nano particles, high selectivity of the molecular-imprinting polymer and high sensitivity of a quartz-crystal microbalance are effectively combined, the cost is low, the synthesis process is simple and the reaction conditions are easily controlled; and the prepared piezoelectric sensor has the advantages of high sensitivity, good reproducibility and long service life and the like, and can be applicable to determination of the content of the ractopamine in a feed sample.

The invention belongs to the field of electrochemical luminescence detection, and particularly relates to an electrochemical luminescence aptamer sensor for detecting ractopamine, a preparation methodand an application method thereof. The electrochemical luminescence aptamer sensor provided by the invention is formed by loading an aptamer on the surface of a glassy carbon electrode modified by anano composite material HKUST-1 / PTC-PEI. According to the invention, the electrostatic interaction of PTC-PEI and HKUST-1 is fully utilized to jointly modify the surface of the glassy carbon electrode, so the flexibility and stability of electrochemical luminescence are obviously improved. The electrochemical luminescence aptamer sensor is obtained by loading the aptamer through the combination effect of the amido bond, so that the target molecule ractopamine can be specifically recognized, and the selectivity of ractopamine detection is improved.

The invention relates to a ractopamine immunogen, a ractopamine coating antigen and use thereof in colloid-gold test paper and belongs to the technical field of immunology. The immunogen and coating antigen are products of the coupling of the hapten of the connecting arm of a hydroxyl terminal far away from a nitrogen atom of the ractopamine with a carrier protein. The hapten is similar to a ractopamine counterpart in terms of molecular structure, stereochemistry and electronic distribution. The molecule of the hapten has an active group for coupling with a protein carrier, and the existence of the active group exerts on influences on the electronic distribution of the molecules of a material to be tested. When coupled with hemocyanin, the hapten enables an organism to produce a high-potency and high-specificity antibody against ractopamine and be absorbed onto a glass cellulose membrane; and the coating antigen constructed by coupling with human haemocyanin can be absorbed onto a nitrocellulose membrane for constructing the colloid-gold test paper.

The invention relates to a ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and a preparation method thereof. The problem of detecting the beta-stimulants the albuterol, the clenobuterol hydrochloride and the ractopamine simultaneously is solved. A parent material PVC plate is sequentially provided with a diversion glass fiber, a carrier glass fiber, a nitrocellulose membrane and a suction cotton pulp plate from front to back; the diversion glass fiber and the suction cotton pulp plate are covered with membranes; the carrier glass fiber absorbs a monoclonal antibody colloidal gold marker resistant to albuterol protein conjugates, clenobuterol hydrochloride protein conjugates and ractopamine protein conjugates; and the intermediate nitrocellulose membrane is provided with a rabbit anti-mouse polyclonal antibody quality control line and three detection lines which are respectively coated with the albuterol protein conjugates, the clenobuterol hydrochloride protein conjugates and the ractopamine protein conjugates. By the invention, the beta-stimulants the albuterol, the clenobuterol hydrochloride and the ractopamine can be effectively detected simultaneously with convenience, quickness and accurate result.

The invention discloses an electrochemical immunosensor capable of detecting various beta-adrenergic receptor stimulants (short for beta-stimulants). The sensor comprises a base electrode, a graphene / chitosan complex membrane and multi-determinant antigens of beta-stimulants, wherein the graphenee / chitosan complex membrane is modified on the surface of the base electrode, and the multi-determinant antigens of the beta-stimulants are fixed on the surface of the graphene / chitosan complex membrane. The multi-determinant antigens fixed on the surface of the sensor are salbutamol-ractopamine-BSA or salbutamol-ractopamine-OVA. The invention also provides an electrochemical immunodetection method for various beta-stimulant residues. The electrochemical immunosensor can be used for detecting six beta-stimulants including clenbuterol, salbutamol, ractopamine, terbutaline, mabuterol and tulobutezol through carrying out cross immune reaction on the beta-stimulants based on wide-spectrum specific antibodies of the beta-stimulants by taking K3[Fe(CN)6] as a probe.

The invention discloses a chemiluminescent enzyme-linked immunosorbent assay (CELISA) kit for detecting ractopamine. The kit comprises a kit body, in which an ELISA plate with wells coated with coating antigen prepared from ractopamine and ovalbumin by coupling, a series of ractopamine standard solutions, enzyme-labeled goat anti-rabbit antibody, anti-ractopamine antibody, a chemiluminescence solution, a washing solution, a coating solution and a blocking solution are arranged. The CELISA kit is sensitive, simple, rapid and accurate; and the sensitivity is improved by one order of magnitude compared with that of the conventional colorimetric ELISA method. The CELISA kit can be used for detecting residual ractopamine in animal-derived foods (such as milk and animal tissues) and urine samples.

The present invention relates to a method of feeding finishing pigs a swine diet which enhances the quality of the meat produced including its drip loss, color, marbling and firmness and thus carcass characteristics. The swine diet includes L-carnitine or salts thereof and ractopamine or salts therof. The amount of the additives present in the diet is such that the quality of the resulting pork and thus carcass characteristics are improved.

The invention provides a chemiluminescent immunoassay kit for detecting ractopamine, and the kit belongs to the field of immunoassay. The kit is composed of a non-transparent white enzyme-labeled plate which is coated by a ractopamine-carrier protein cross-linked complex, a ractopamine standard product, a ractopamine-peroxidase-labeled antibody, luminescent substrate solution and concentrated buffer solution. The ractopamine-carrier protein cross-linked complex is obtained by coupling the ractopamine and a carrier protein through the mixed-anhydride method or the direct protein activation method, and the concentrated buffer solution contains Tween-20 buffer solution. The kit has the advantages of rapidness, simpleness, high sensitivity, low detection cost, good repeatability, high throughput and the like, and the kit can be used for detecting the residual ractopamine in animal urine, blood, tissues, visceral organs and other samples.

A process for preparing the compound Laikeduoba amine from frambone and omega-bromo-p-hydroxyacetophenone includes three steps. Its advantages are no need of noble metal catalyst and catalytic hydrogenation, and high output rate.

The invention discloses a method for detecting ractopamine and a chemiluminescence immunoassay kit special for the same. The chemiluminescence immunoassay kit for detecting the ractopamine provided by the invention comprises a ractopamine specific antibody, a coating antigen and standard solution, wherein the coating antigen is a conjugate of ractopamine hapten and carrier protein. The method for detecting the ractopamine has the characteristics of simple pre-processing process of a sample, simple and convenient operation, low cost, high specificity, high flexibility, high accuracy and the like, on-site monitoring can be preformed by the method and the method is suitable for screening of a great number of samples.

The invention discloses an immunochromatographic kit for carrying out fluorescence quantitative on ractopamine through and a preparation method of a fluorescence labeling liquid. The kit comprises a test strip and the fluorescence labeling liquid, wherein the test strip is formed by sequentially overlapping and adhering a sample pad, a nitrocellulose coating film, and absorbent paper on a bottom plate; the nitrocellulose coating film comprises a detection region and a quality control region; the detection region is coated with an RCT-BSA (antibody against Ractopamine-bovine serum albumin) conjugate, the quality control region is coated with anti-rabbit IgG; and the fluorescence labeling liquid contains a fluorescence labeling RCT antibody and a fluorescence labeling rabbit IgG. Compared with the immune colloidal gold labeling test strip, the kit has the advantages of higher sensitivity, accurate quantization and the like, and is more convenient and rapidly in operation in comparison with an enzyme coupling method.