This invention discloses a three-dimensional multilayer sheet-like nanozyme, its preparation method, and its application in the immunochromatographic detection of
small molecule environmental pollutants, belonging to the field of nanozyme and immunochromatographic detection technology. The three-dimensional sheet-like nanozyme is meticulously designed by continuously constructing Au nanoparticles (NPs) and AuPt bimetallic NPs on flexible
graphene oxide (GO) nanosheets. Beneficial effects: In the nanozyme, two-dimensional GO provides a large surface area and high stability, hundreds of large AuNPs provide enhanced colorimetric ability and a large spherical surface area, and tens of thousands of AuPt satellites act as spatial catalytic sites and produce excellent
peroxidase-like activity. The proposed bimodal ICA can simultaneously detect three important drugs, namely
gentamicin,
clenbuterol, and
ractopamine, in real complex samples, greatly improving the
detection performance of existing colorimetric ICA techniques based on simple
catalysis, thus showing great application potential in real-time monitoring of
small molecule targets under different conditions.