11 results about "Ractopamine" patented technology

The invention discloses a three-dimensional multi-layer flaky nano-enzyme, a preparation method thereof and an application of the three-dimensional multi-layer flaky nano-enzyme in immunochromatography detection of micromolecular environmental pollutants, and belongs to the technical field of nano-enzymes and immunochromatography detection. The three-dimensional flaky nano enzyme is elaborately designed by continuously constructing Au nano particles (NP) and AuPt bimetallic NP on a flexible graphene oxide (GO) nano sheet. The nano-enzyme has the beneficial effects that in the nano-enzyme, the two-dimensional GO provides a huge surface area and high stability, hundreds of large AuNPs provide enhanced colorimetric ability and a large-area spherical surface, and tens of thousands of AuPt satellites serve as space catalytic sites and generate excellent peroxidase-like activity. The dual-mode ICA provided by the invention can be used for simultaneously detecting three important drugs, namely gentamicin, clenbuterol and ractopamine, in a real complex sample, and the detection performance of the existing colorimetric ICA technology based on simple catalysis is greatly improved, so that the dual-mode ICA shows huge application potential in the aspect of monitoring small molecular targets in real time under different conditions.

The invention relates to a surface-imprinted combined modified electrode and its production method, comprising a novel chalcone-substituted cyclotriphosphazene (CBRG-Fe) and multi-walled carbon nanotube (MWCNT) composite, intended to be used as an electrochemically active sensor for the sensitive determination of ractopamine.

The invention belongs to the technical field of food detection, and particularly relates to a preparation method of deuterium labeled ractopamine hydrochloride-d5. The method comprises the following steps: by taking deuterated phenol as a raw material, carrying out a series of reactions of substitution, phenolic hydroxyl group protection, reductive amination, deuterated lithium aluminum hydride reduction, palladium carbon reduction, deprotection and salification to obtain deuterium-labeled ractopamine-d5; the reaction process is good in atom economy, simple and convenient in synthesis operation and good in reproducibility and stabilizer, can be used as an internal standard substance in the field of food quality inspection, and has important practical application value.

The invention discloses a preparation method of a beef frozen wet powder matrix standard substance, and solves the technical problems of poor authenticity, low fidelity, poor uniformity and high energy consumption when a beef ractopamine residual matrix standard substance is prepared in the prior art. Obtaining a ractopamine positive beef sample; performing acid discharge treatment on the beef sample; pretreating the beef subjected to acid discharge, and cutting the beef into diced meat with the particle size of 5-10mm; preparing a frozen wet powder matrix standard substance by adopting a multi-stage freezing and crushing and pulse screening process; and uniformly mixing the final product at a low temperature, sub-packaging, and storing in an environment of-40 DEG C or-80 DEG C in a sealing manner. The traditional freeze-dried powder process is abandoned, the frozen wet powder is used as a carrier form, and the original physicochemical characteristics of fresh tissues are perfectly reserved by the form.

The invention relates to the technical field of kits, in particular to a kit for rapidly detecting ractopamine. Comprising a placement box body, a reagent tube, a detection tube arranged on the upper surface of the placement box body and communicated with the interior of the placement box body, a water bath kettle arranged below the detection tube and located in the placement box body, and a supporting device arranged on one side of the water bath kettle and used for supporting, a test device for detecting whether the weight of a sample in the reagent tube reaches the standard or not is arranged in the detection tube, the detection tube is used for containing the reagent tube, and a through opening penetrating through the containing box body is formed in one side of the containing box body. Then a sample is added into the reagent tube, after the added sample reaches 4 g or above, the testing device is started, the reagent tube falls into the water bath kettle and is supported by the supporting device, after cooking is completed, the supporting device is started, the reagent tube is taken out, the detection step is omitted, and detection of detection personnel is more convenient.

This invention discloses a three-dimensional multilayer sheet-like nanozyme, its preparation method, and its application in the immunochromatographic detection of small molecule environmental pollutants, belonging to the field of nanozyme and immunochromatographic detection technology. The three-dimensional sheet-like nanozyme is meticulously designed by continuously constructing Au nanoparticles (NPs) and AuPt bimetallic NPs on flexible graphene oxide (GO) nanosheets. Beneficial effects: In the nanozyme, two-dimensional GO provides a large surface area and high stability, hundreds of large AuNPs provide enhanced colorimetric ability and a large spherical surface area, and tens of thousands of AuPt satellites act as spatial catalytic sites and produce excellent peroxidase-like activity. The proposed bimodal ICA can simultaneously detect three important drugs, namely gentamicin, clenbuterol, and ractopamine, in real complex samples, greatly improving the detection performance of existing colorimetric ICA techniques based on simple catalysis, thus showing great application potential in real-time monitoring of small molecule targets under different conditions.

The present application relates to the technical field of bioengineering, and particularly relates to a rabbit monoclonal antibody of ractopamine and a preparation method and application thereof.The rabbit monoclonal antibody comprises an antibody heavy chain and an antibody light chain, the heavy chain variable region of the rabbit monoclonal antibody comprises an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2, and the light chain variable region of the rabbit monoclonal antibody comprises an amino acid sequence as shown in SEQ ID NO.3 or SEQ ID NO.4.A rabbit monoclonal antibody for detecting ractopamine is obtained by the present application, the rabbit monoclonal antibody has high tolerance to urea, and has important significance in the field of detecting ractopamine in animal urine.

The invention discloses a double-quantum-dot microsphere fluorescence immunochromatography kit for detecting clenbuterol and ractopamine as well as a preparation method and a detection method of the double-quantum-dot microsphere fluorescence immunochromatography kit, and relates to the technical field of immunochromatography. The fluorescent test strip based on the double quantum dot microsphere-monoclonal antibody conjugate is prepared, clenbuterol and ractopamine can be simultaneously detected on one test strip, and the detection flux of a single test strip is improved; two antibodies are respectively marked by the bicolor quantum dot microspheres, so that fluorescence interference between two T lines during reading can be effectively avoided, and meanwhile, the problem that a C line of a multi-component immunochromatography test strip is difficult to judge whether a single antibody is effective or not is solved. The detection method provided by the invention has relatively good specificity, accuracy and repeatability. Therefore, the method has an excellent application prospect in the field of high-sensitivity multi-component immunoassay.

The invention relates to a method for determining the contents of 15 beta-receptor stimulants, 3 amido alcohols and 11 nitroimidazole antibiotics in agricultural products, and belongs to the technical field of detection. The invention relates to a method for determining the contents of 15 beta-receptor stimulants, 3 amido alcohols and 11 nitroimidazole antibiotics in agricultural products. The agricultural products are spinach, asparagus lettuce or peach. The 15 kinds of beta-receptor stimulant antibiotics are cimaterol, cibuterol, ritodrine, clenbuterol, hydroxymethyl clenbuterol, clenproterol, ractopamine, clorprenaline, clenbuterol, tubuterol, brombuterol, bambuterol, mabuterol, clenbupant and mappenterol, and the 15 kinds of beta-receptor stimulant antibiotics are selected from the group consisting of propamine, propamine, propamine, propamine, propamine, propamine, propamine, propamine, propamine, propamine, propamine, propamine, propamine and propamine. The three kinds of amide alcohol antibiotics are chloramphenicol, thiamphenicol and fluorine thiamphenicol. The method has the characteristics of simplicity, convenience, high efficiency, accuracy, sensitivity, economy and the like.

The application provides a high-sensitivity detection method for multi-residues of veterinary drugs in pig urine, and belongs to the technical field of detection of veterinary drug residues. The application first performs enzymatic treatment on the sample to be detected, thereby improving the detection sensitivity of salbutamol and ractopamine; then performs acid hydrolysis and derivatization treatment on the sample to be detected, thereby improving the reversed-phase chromatographic retention performance and mass spectrometry ionization efficiency of nitrofurans in the compound to be detected, improving the sensitivity, improving the polarity of the compound to be detected, thereby improving the chromatographic separation effect, effectively separating the interfering substances in the matrix, improving the anti-interference ability of the detected substance, and indirectly improving the detection sensitivity; clenbuterol, metronidazole, dimetridazole, olaquindox, chlorpromazine and chloramphenicol are mainly in free state, and are not affected by enzymatic and acid hydrolysis and derivatization during detection, can be detected together with the above-mentioned compounds to be detected, and the mass spectrometry derivatization and sensitivity detection of multi-residues of cross-class veterinary drugs are realized.

The utility model discloses a ractopamine fluorescent quantitative detection reagent box, including the outer fixed frame, the inner side of outer fixed frame inserts and installs the reagent box, the inside of reagent box upper side is equipped with the first storage cavity, the inside insertion of first storage cavity is equipped with the agitator box, the bottom of agitator box inside is equipped with motor fixed cavity, the bottom fixedly connected with micro motor in the inside of motor fixed cavity, the periphery fixedly connected with the baffle of micro motor, the output of micro motor upper end penetrates the baffle and extends to the upside in the inside of agitator box, the output of micro motor upper end is connected with a plurality of stirring vane. The utility model discloses through with the fluorescein pours into to the agitator box in the inside of first storage cavity, opens the switch of micro motor again, the rotating shaft on micro motor drives rotating shaft to carry out the rotary motion, and the rotating shaft drives the stirring vane to carry out the rotary motion, so that the stirring vane mixes and mixes the ractopamine antigen liquid of collection to fluorescein.