The invention discloses a
royal jelly freshness detection design matching method based on a structure
diffusion model, and relates to the technical field of
food quality detection. The invention discloses a
royal jelly freshness detection design matching method based on a structure
diffusion model. The method comprises the steps of sample acquisition,
peptide fragment analysis,
peptide fragment reverse target searching,
binding energy calculation and detection design matching
system construction. According to the method,
protein and degradation products thereof related to
royal jelly freshness can be quickly identified by virtue of
a peptide fragment reverse target searching method and a
mass spectrometry and
database matching technology, so that the time for searching related
protein markers is greatly shortened, and the freshness detection efficiency is remarkably improved; by accurately matching
protein sequences and identifying degradation products, it is ensured that screened markers are closely related to the
degradation process of royal jelly, and therefore scientificity and accuracy of freshness detection are improved; through utilization of royal jelly
proteome information,
data analysis efficiency and
cost effectiveness are improved.