11 results about "Thiamphenicol" patented technology

The invention provides a method for detecting enantiomers of thiamphenicol glycine ester hydrochloride or thiamphenicol glycine ester hydrochloride in a preparation thereof. The method comprises the following steps: preparing a test solution: taking thiamphenicol glycine ester hydrochloride or a preparation thereof to prepare a test solution; chromatographic conditions: adopting chiral chromatography with cellulose tris-(4-chloro-3-methylphenylcarbamate) as a stationary phase and n-hexane:methanol:anhydrous ethanol:trifluoroacetic acid:ethanolamine as a mobile phase; and a determination method: taking the test solution and injecting it into a liquid chromatograph for determination.

The application discloses an antibody against thiamphenicol and / or florfenicol, a detection product and a method for detecting thiamphenicol and / or florfenicol, and relates to the technical field of thiamphenicol / florfenicol detection. The thiamphenicol or florfenicol monoclonal antibody provided by the application has high specificity for thiamphenicol or florfenicol, and has high detection sensitivity and a wide linear detection range for thiamphenicol or florfenicol. The application is helpful for rapidly detecting thiamphenicol and florfenicol antibiotic residues in livestock and poultry products and aquatic products.

The present invention discloses a thiamphenicol hapten, an artificial antigen, an antibody, and a preparation method and application thereof. The thiamphenicol hapten structural formula is shown in formula (I). An artificial antigen is prepared based on the hapten to immunize an animal to obtain a monoclonal antibody; a thiamphenicol colloidal gold immunochromatographic test strip is prepared based on the antibody to detect the IC value of thiamphenicol. 50 The detection limit of the present invention is 0.70 ng / mL, the linear range is 0.16-3.17 ng / mL, and the minimum detection limit is 0.06 ng / mL, which meets the limit of thiamphenicol in GB 31650-2019. It shows that the artificial antigen prepared by the present invention has good immunogenicity, can be used to prepare thiamphenicol monoclonal antibodies and establish corresponding immunoassay methods, and is suitable for on-site high-throughput rapid detection of thiamphenicol in food. The antigen and antibody preparation process of the present invention are simple, low in cost, highly sensitive, and have a low detection limit, and have good application prospects.

The invention provides a time-resolved fluorescence immunochromatography method and kit for detecting thiamphenicol, and belongs to the field of analytical chemistry. The kit mainly comprises a time-resolved fluorescent microsphere labeled antibody probe and a test strip, the time-resolved fluorescent microspheres are prepared by embedding europium complexes into polystyrene microspheres through a swelling method. Performing chemical coupling on the time-resolved fluorescent microspheres and a thiamphenicol monoclonal antibody to prepare a time-resolved fluorescent microsphere labeled antibody probe; the test strip comprises a nitrocellulose membrane, a sample pad and an absorption pad which are arranged on a bottom plate, and the nitrocellulose membrane is coated with a detection line T of thiamphenicol antigen and a quality control line C of goat anti-mouse IgG antibody. The invention provides a time-resolved fluorescence immunochromatography method for detecting thiamphenicol and a kit, which can be applied to detection of thiamphenicol in food samples and have the characteristics of simplicity, convenience, high sensitivity, rapidness in quantification and the like.

The utility model relates to a component detection device for an enteric-coated tablet, and aims to solve the technical problems that pollution is easily caused in the process of transferring the ground enteric-coated tablet at present, and thiamphenicol in the enteric-coated tablet is easily decomposed when being transferred and ground due to the fact that the thiamphenicol is easily decomposed when being exposed to light, so that the component detection result of the enteric-coated tablet is influenced. Comprising a grinding pipe, an extraction pipe, a filter pipe and a storage pipe, the grinding pipe, the extraction pipe, the filter pipe and the storage pipe are sequentially connected from top to bottom, the top of the grinding pipe is connected with a cover plate through a bolt, a driving motor is installed on the cover plate, a grinding mechanism is arranged in the grinding pipe and connected with the driving end of the driving motor, and the driving end of the driving motor is connected with the extraction pipe. According to the utility model, the grinding, extracting and filtering of the enteric-coated tablet can be continuously completed in a sealed space, and the treated sample does not need to be transferred, so that the influence on the component detection result of the enteric-coated tablet caused by pollution and photolysis of thiamphenicol in the enteric-coated tablet in the transferring process is avoided.

The invention provides an application of thiamphenicol in preparation of a preparation for resisting the porcine Seneca virus disease, so that the porcine Seneca virus disease can be effectively prevented and treated. The invention also provides a product for preventing and / or treating SVA infection, and the product contains thiamphenicol with pharmacological effective concentration. The invention provides the application of the thiamphenicol in preparing the preparation for preventing and / or treating SVA infection, the thiamphenicol has a very obvious anti-SVA infection effect, cell infection caused by SVA can be reduced, adsorption, cell entry and release of SVA can be inhibited, and a new component choice is provided for clinical treatment of the porcine Seneca virus disease.

The invention discloses an antibody resisting thiamphenicol and / or florfenicol, a detection product and a method for detecting thiamphenicol and / or florfenicol, and relates to the technical field of thiamphenicol / florfenicol detection. Immunodetection experiments prove that the thiamphenicol or florfenicol monoclonal antibody provided by the invention has high specificity on thiamphenicol or florfenicol, the detection sensitivity on thiamphenicol or florfenicol is high, and the linear detection range is wide. The rapid detection of thiamphenicol and florfenicol antibiotic residues in livestock and poultry products and aquatic products is facilitated.

This invention relates to a chiral method for preparing thiamphenicol. Specifically, the method uses p-methylsulfonylbenzaldehyde and glycine ester as starting materials, and carries out an asymmetric aldol reaction in the presence of a pyridinium salt carbonyl catalyst, followed by a reduction and amidation reaction to obtain thiamphenicol. This invention utilizes the strong catalytic ability of small-molecule pyridinium salt carbonyl catalysts, starts with inexpensive and readily available raw materials, reduces the steps of introducing and removing protecting groups, shortens the synthetic route, and efficiently synthesizes optically pure thiamphenicol. Furthermore, the preparation method of this invention is simple to operate and environmentally friendly.

The invention discloses a chloramphenicol degrading strain CS1 and application thereof in repairing the environment polluted by chloramphenicol, thiamphenicol and nitrobenzene compounds, the chloramphenicol degrading strain CS1 is preserved in China Center for Type Culture Collection on March 10, 2025, the preservation number is CCTCCM2025425, and the preservation address is Luojia mountain on eight road of Wuchang District, Wuhan City, Hubei Province. The chloramphenicol degrading strain CS1 disclosed by the invention can be used for completely removing the chloramphenicol which is up to 100 mg / L in an inorganic salt culture medium within 150 minutes. The strain keeps efficient chloramphenicol degradation activity in a water body at the temperature of 15-50 DEG C and the pH value of 4-11. Meanwhile, the strain can degrade chloramphenicol in soil within a short time, and long-acting chloramphenicol degradation activity is kept. The invention can be used for solving the problem of harm of residual chloramphenicol to soil environment, crops and human health.

The invention relates to a preparation method and application of a MoS2 (at) CoFe-LDH (at) Ni-MOF composite material. The catalyst is synthesized by taking CoFe-LDH coated Ni-MOF as a precursor and adopting a hydrothermal method, and is of a six-petal snowflake-shaped branch structure. In a degradation experiment aiming at a target pollutant thiamphenicol, the composite catalyst shows excellent performance. The test result shows that the MoS2-loaded MoS2 (at) CoFe-LDH (at) Ni-MOF catalyst has the degradation rate of about 70% in the test period of 10 days. Compared with the prior art, the cathode of a control group which is not loaded with the catalyst can only reach the degradation rate of about 55% within the same time. The system loaded with the catalyst significantly improves the degradation rate, and shortens the time required for achieving a high removal rate. The catalyst shows remarkable technical advantages and good application prospects in the field of electrochemical removal of organic pollutants in water.

The invention relates to a ZIF-67 (at) CoFe-LDH (at) Ni-MOF catalyst, which is characterized in that lamellar Ni-MOF is used as a precursor, and the ZIF-67 (at) CoFe-LDH (at) Ni-MOF electrocatalyst is prepared by a hydrothermal synthesis method. According to the method, thiamphenicol is taken as a target pollutant, and the degradation performance of the composite catalyst within 10 days is measured. Results show that compared with other catalysts, the CoFe-LDH coated Ni-MOF composite material shows the strongest antibiotic degradation performance, the concentration of TAP is reduced to 0.55 mg / L when a cathode is in 2 d, and ZIF-67 coated CoFe-LDH coated Ni-MOF shows an excellent electrocatalytic degradation effect. The degradation rate within 10 days is 90% respectively. Compared with a cathode control group which is not loaded with the catalyst and can only reach the degradation rate of about 55% within 10 days, the system loaded with the catalyst (especially CoFe-LDH-coated Ni-MOF) obviously shortens the time required for achieving high degradation. The method has remarkable technical advantages and wide application prospects in the field of electrochemically removing organic pollutants in water.