94 results about "Facultative" patented technology

The invention relates to enterococcus faecium for feeding as well as a freeze-drying fungicide and applications thereof, and discloses enterococcus faecium LAB12 CGMCC (China General Microbiological Culture Collection Center) No.4847 which is grampositive cocci, has no spores, grows well on an MRS agar plate, forms a round bacterial colony with the diameter of 0.5-1mm within 48 hours and is used for feeding, and the bacterial colony is round, smooth and upheaved, and is a shape of grey white dewdrop; the enterococcus faecium grows in a facultative anaerobic condition; the growth temperature range is 10 DEG C-45 DEG C; the optimum growth temperature is 30 DEG C-40 DEG C; and the growth pH value is 4-10, and the optimum pH value is 6.0. The freeze-drying fungicide formed by the bacterial strain is nontoxic and harmless, is gastric juice resistant, is cholate resistant, has a high inhibitory effect for multiple harmful bacteria, has a long quality guarantee period, can be widely applied to birds and livestock breeding to strengthen the animal disease-resistant capability, and is expected to be the substitution of antibiotics for feeding.

The invention discloses an FQ15 enterococcus faecalis and a method of a feed additive which uses the bacteria production for promoting the growth: an MRS culture medium is taken as a seed culture medium, and the FQ15 enterococcus faecalis is cultured under the aerobic or facultative condition at 30 to 45 DEG C for 4 to 24 hours, so as to become a grade one seed; the grade one seed liquid is inoculated in a seed tank for amplification culture, the MRS culture medium is adopted as the seed culture medium, and the culture is carried out for 4 to 24 hours under the aerobic or the facultative condition at 30 to 45 DEG C to become a grade two seed; the grade two seed liquid is inoculated in a fermentation tank for fermentation culture, an improved MRS culture medium is adopted as the fermentation culture medium, and the culture is carried out for 8 to 36 hours by using timing or continuous fed-batch fermentation mode under the aerobic or the facultative condition at 30 to 45 DEG C; the obtained fermentation liquid is sub-packaged or the fermentation liquid which is obtained by step c is separated, 1 to 25 percent drying protector is added in the bacterial sludge, and the granulation at 20 - 80 DEG C, freeze-drying or spray drying are adopted. The invention has the advantages that the invention can substitute the feed antibiotics and improve the high efficient weight increase of livestock and poultry; the production process is simple; the cost is low, etc.

The invention discloses a method for constructing genetic engineering bacterial strain Escherichia coli JM125 producing boletic acid and method for producing acid. The constructing process comprises inactivation or out-knocking enzymes in facultative microbiology which has abilities of reduction boletic acid and degrading acetonic acid, and exceeding expressing NAD regeneration. The following steps of two-stage fermentation experiment using constructed Escherichia coli are (1) activating bacillus coli is transferred to enrichment medium and cultured to improve biomass. (2) The treated bacterium liquid membrane obtained by aerobic cultivation is transferred into LB culture medium which contains 20 to 100g/L amylaceum and certain concentration carbonates to produce fumaric acid by anaerobic fermentation.

The invention discloses anaerobic degradation cellulose methane composite bacteria, which is composed of 13 strains that respectively belong to facultative anaerobic and strictly anaerobic fermentative bacteria, hydrogen-production and acetic acid-production bacteria and methane-producing bacteria. The composite bacteria can not only effectively perform anaerobic degradation to the cellulose, but also can use the degradation production of the cellulose to produce methane, which can be widely used in straw biogas fermentation, compost processing, and the like conversion processes.

The invention relates to an anaerobic-aerobic integrated microbial fuel cell wastewater treatment system. Facultative power-generating bacteria and facultative degrading bacteria are constructed in an anode chamber of a microbial fuel cell and the alternate anaerobic-aerobic operation mode is adopted, thus the aim of utilizing the anaerobic power generation of the organic matter and realizing aerobic degradation can be achieved. By adopting the system, the problem that when the past microbial fuel cell system utilizes organic wastewater to generate power, the degradation of the organic matter is not complete, can be overcome and the resource utilization and harmless emission of organic wastewater can be realized. The system has the following specific characteristics: the fuel cell system comprises a cathode chamber, the anode chamber, an anode in the anode chamber, a cathode in the cathode chamber, a diaphragm between the cathode and the anode and aeration headers arranged in the cathode chamber and the anode chamber; the facultative power-generating microorganisms are attached to the anode of the anode chamber, the facultative degrading microorganisms exist in the solution of the anode chamber; and the alternate anaerobic-aerobic operation mode is adopted to realize wastewater power generation under the anaerobic condition and the rapid degradation of the organic matter under the aerobic condition.

The invention discloses a strain of facultatively anaerobic denitrifying bacteria, and the strain is named as Pseudomonas stutzeri LZ-14 and belongs to the pseudomonas stutzeri of pseudomonas. The strain was collected in the China Center for Type Culture Collection on April 10th, 2011, wherein the collection number is CCTCC No.M2011119. The strain can be used for effectively removing ammonia nitrogen, nitrate nitrogen and nitrite nitrogen as well as a mixture thereof so as to reduce the total concentration of nitrogen, and can be used for removing CODCr (chemical oxygen demand) in organic wastewater at the same time, thereby being suitable for treating organic wastewater of relatively low concentration, nitrite of relatively high concentration or nitrate wastewater and having wider adaptability. In the denitrification process, nitrate accumulation is avoided, while a certain amount of nitrite accumulates but can be completely degraded in 24 hours later. By using the strain, the wastewater treatment process is simple and the denitrification effect is stable. If the bacteria is used for enhancing biological removal of pollutants in water, an LZ-14 bacteria liquid is added when the bacteria stays for 8 days so as to enhance the biological removal effect, so that the CODCr in the polluted river water is reduced from 33.9 mg/L to 15.9 mg/L at a removal rate of 53.1%; and TN is reduced from 4.07 mg/L to 0.98 mg/L at a removal rate of 75.9%.

A method and apparatus for sequentially nitrifying ammonia in landfill leachate ex situ and then returning the nitrified landfill leachate to the landfill where the leachate is denitrified.

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

The present invention is directed to the seed-to-seed perpetuation of hybrid vigor and other traits through apomixis (asexual seed formation) in flowering plants (angiosperms). More particularly, to predictable methods for producing, from sexual or facultatively-apomictic plants, progeny plants that express an increased percentage of apomictic seed set or one or more elements of apomixis. This invention uses: plant cyto-embryology procedures to identify and select a plant or group of plants that possess appropriate genetic variability for initiation times and durations of megasporogenesis (female meiosis), embryo sac formation, egg and central cell formation and maturation, fertilization, embryony and endosperm formation; plant breeding procedures to produce numerous and divergent genetically-recombined early to late generation progeny such that embryo sac formation preempts megasporogenesis and embryony preempts fertilization; and plant cyto-embryology or progeny test procedures to select segregant plants that express an increased frequency of one or more elements of apomixis.

The invention relates to a preparation method and application of a microbial inoculant for remediating poly-halohydrocarbon-polluted soil, belonging to the technical field of soil remediation in environmental engineering. The invention is characterized in that the microbial inoculant containing the quinone-reducing facultative anaerobe Shewanella sp. and poly-halohydrocarbon dehalogenated product facultative aerobic degrading bacterium is prepared by surplus sludge lysis solution preparation and culture medium optimization, and is used for remediating poly-halohydrocarbon-polluted soil. The microbial inoculant has the advantages of wide raw material sources, simple preparation technique and no secondary pollution during soil remediation, can effectively overcome the defect that the high operating cost of the biologically reinforced remediation of poly-halohydrocarbon-polluted soil limits the large-scale application, and has wide application prospects in remediation of soil polluted by poly-chlorine organic substances, poly-bromine aromatic hydrocarbons and poly-fluorine organic substances.

The invention relates to a new strain golden Brevibacterium strain AN3 CGMCC No.2193, and application thereof in degrading triphenyl methane dyes and azo dyes such as malachite green. The golden Brevibacterium strain AN3 is obtained by separating and purifying activated sludge in an anaerobic baffled reactor system used for treating printing and dyeing wastewater by an research institute in Guangzhou, can degrade the triphenyl methane dyes in a mode of benzene ring detoxication under a facultative anaerobic condition that the temperature is between 25 and 37 DEG C and the pH value is between 6.0 and 8.5, and has significant capability on degrading the prior triphenyl methane dyes and azo dyes such as the malachite green which bring about serious pollution to aquatic products and are used in the form of a fungicide, wherein the removal rate of the golden Brevibacterium strain to the malachite green with the concentration of 50 milligrams per liter is 61.4 percent in 8 hours and is 97.3 percent in 16 hours, and the highest tolerable concentration thereof is 500 milligrams per liter, so that the strain golden Brevibacterium strain can effectively solve the problems of pollution and purification caused by the malachite green in an aquaculture environment.

This invention opens a hair iron reduction Cong Aeromonas and its applications. After a lot of inventors experimental study, and selected a hair iron reduction Aeromonas Cong, a reduction of humus and iron reduction activity. This reduction of citric acid to iron, ferric hydroxide, water iron ore, goethite three-valent iron; can restore humus and humus model of AQDS (anthraquinone 2 ,6 - disulfonic acid). Iron reducing gross Cong Aeromonas facultative anaerobic, the electronic spectrum for the broader use of this bacterium in the anaerobic oxidation conditions to the electronic donor: glycerol, glucose and sucrose.

The invention discloses a method for improving the hydrogen production quantity of photosynthetic microalgae through phycomycetes co-culture. Particularly, green alga and one kind of facultative anaerobic/facultative chemoautotrophic thiomonas intermedia are proportionally mixed for culture; oxygen released by green alga photosynthesis can be consumed by the bacterium respiration effect; the carbon dioxide released through the bacterium respiration effect can be supplied to the green alga for better performing photosynthesis effect, so that the anaerobic characteristics of the whole culture environment can be well maintained. In addition, the balance catalysis capability of the thiomonas intermedia on the sulfur element is utilized; the limited supply of the sulfur element can be realized,so that the normal growth of the green alga can be ensured; the efficient durable hydrogen production can be realized.

The invention relates to the technical field of microbes, in particular to lactobacillus acidophilus and a screening method and application thereof and a microbial inoculum. The collection number of the lactobacillus acidophilus is CGMCC NO.14437; the lactobacillus acidophilus has a strong glucosinolate degrading function, and can be applied to detoxification of rapeseed meal by independently serving as a fermenting microbial inoculum; when the lactobacillus acidophilus is applied to a rapeseed meal fermenting process, the lactobacillus acidophilus can promote production of polypeptide and lactic acid and has strong abilities of producing acid and inhibiting harmful bacteria; as a broiler feed additive, the lactobacillus acidophilus can promote nutrient digestion and absorption of animals,inhibit proliferation of pathogenic bacteria in gastrointestinal tracts of the animals and promote the balance of intestinal microflora. The lactobacillus acidophilus is a facultative anaerobic microorganism, and is strong in environmental applicability, high in growth speed, tolerable to high-concentration glucosinolate and high in safety.

The invention provides a production process for synthesizing 1,3-propanediol by a biological method, and belongs to the technical field of biochemical engineering. The process adopts microbe aerobic fermentation, uses amphimicrobian Klebsiella pneumoniae as a starting strain, and comprises the following steps in turn: activating seeds of the Klebsiella pneumoniae; culturing the seeds under an aerobic condition; under a microaerobic condition, using thallus of the Klebsiella pneumoniae as a biocatalyst, and fermenting a glycerinculture-medium assisted by glucose to produce the 1,3-propanediol under conditions that the fermenting temperature is between 30 and 50 DEG C and pH is between 7 and 9; and finishing fermentation after fermenting for 18 to 36 hours. The method for synthesizing the 1,3-propanediol has the characteristics of short production period, high production strength, simple fermenting control, convenient operation and low cost.

The invention relates to aerobic type facultative autotrophic denitrifying bacteria and application thereof. The aerobic type facultative autotrophic denitrifying bacteria are Pseudomonas koreensis Y5-11 which is preserved in China general microbiological culture collection center, the preservation address is No.3, No.1 Beichen West Road, Chaoyang District, Beijing, the preservation date is October 29, 2018, and the preservation number is 16651. The strain of the aerobic facultative autotrophic denitrifying bacteria can be grown by using an organic carbon source, or CO2 can be used as a sole carbon source to reduce nitrate. The aerobic facultative autotrophic denitrifying bacteria are applied to the field of environmental microorganisms.

The present invention is directed to a medium, broth or agar, and a method of utilizing the same, in order to isolate and/or identify anaerobes from a mixed sample that contains facultative microorganisms. The medium contains an inhibitor of the electron transport system, such as a salt of azide (N₃⁻), cyanide (CN⁻) or related compounds. These inhibitors are present in an amount sufficient to limit the growth of facultative microorganisms under anaerobic conditions while not inhibiting the growth of the anaerobe microorganisms. Preferably, the inhibitor is present in the amount of from about 0.1 mg/ml to about 1.0 mg/ml in broth medium, and from about 0.01 mg/ml to 1.0 mg/ml in agar medium.

The invention relates to a method for remediating uranium contaminated water with an imprinted fungal material having high affinity and high recognition capability to uranyl ions. An uranium ion imprinted material is prepared by means of the sol-gel technique from a typical facultative marine mangrove endophytic fungus Fusarium sp.#ZZF51 as a matrix, phytic acid as a functional monomer, UO2<2+> as a template ion, and tetraethoxysilane (TEOS) as a cross-linking agent; then, the obtained material is thrown in uranium contaminated water to remediate the uranium contaminated water; and after a period of time, the material is retrieved and subjected to centralized treatment. The material proposed provided by the invention has high affinity and high recognition capability to uranyl ions, and may lead to a removal rate of uranyl ions of 90% or above 50-70 min later after being thrown in water. The imprinted fungal material is in the form of spherical granules, and is low in cost, high in remediation efficiency, environmentally friendly without secondary pollution, and relatively simple and convenient in retrieval and centralized treatment.

Directed at the current situation of the prior art and based on a circulating water flushing type ecological toilet, the invention aims to realize self-circulation, no emission, no picking and complete decomposition of water flushing type toilets. Through a current reactor and by the mode of anaerobiosis, anoxia, aerobiosis, anoxia, and anaerobiosis, a biological rotating disc is designed into a facultative pond with a special structure. A wood fiber bio-carrier bed is employed, and an ultrasound technology is adopted to perform a new internal circulation stirring process in the biological rotating disc facultative pond, an upflow type anaerobic tank, and an anoxic tank so as to accelerate decomposition and digestion of organic matters. Meanwhile, electro-adsorption, high-energy physics, an anaerobic and aerobic wood fiber integrated facultative tank and other technologies are adopted to conduct water treatment on fecal sewage. The invention can achieve through and complete decomposition of organic matters and eliminate malodor. The bio-environmental protection toilet needs no water supply, and the first supplied water can be circulated for use, so that no more water needs to be added. The toilet provided in the invention is an environmental protection toilet having no residue, precipitate, emission or pollutant.

The invention discloses a facultative anaerobic denitrifying bacterium with self-flocculation capability. The bacterial strain is named as Pseudomonas stutzeri LZ-4 and belongs to pseudomonas stutzeri strain, and the preservation number is CCTCCM 2011430. The bacterial strain provided by the invention can be applied to the denitriding of waste water, and the optimal denitriding temperature of thebacterial strain is 20-35 DEG C. The bacterial strain adapts to a wide pH value range which is between pH 6 and pH 11. When a C/N (mol/mol) ratio is more than or equal to 3, the denitriding rate of the bacterial strain is up to above 90%. In a slight oxygen solution environmental condition, the denitriding activity of the bacterial strain is highest. Final product gas of denitrification is authenticated as nitrogen, and inorganic nitrogen such as nitrate nitrogen, nitrite nitrogen and the like in a water body can be directly reduced into the harmless nitrogen to be discharged outside the water body. By using the bacterial strain, at a later period when a denitrification role is played, the aggregation and the formation of a flocculating constituent are started, and the flocculating constituent is precipitated at the bottom of the waste water. Through contrasting the performance detection of the bacterial strain, the following effects that a process of treating nitrogen-containing waste water by using the bacterial strain is simple, a treatment effect is efficient and stable, further, greenhouse gases such as NO and N2O cannot be generated, and the air pollution cannot be caused can be realized.

The invention relates to the technical field of sewage treatment and provides a facultative microalgae photobioreactor sewage purification system. The facultative microalgae photobioreactor sewage purification system comprises a bioreactor, a vertical light distribution device, an aeration device, an algae residue collector and facultative microalgae. The system comprises the vertical light distribution device and the aeration device, during applying, the interior of the system can be supplemented with air and a light source, thus the optimal growth conditions are provided for the facultativemicroalgae, the facultative microalgae grows industrially, and thus sewage is effectively purified through a large amount of the microalgae. The invention further provides a sewage purification method, the method is simple in step, aquaculture wastewater and domestic sewage can be purified in a targeted mode, and pollution treatment is clean and effective.

The invention provides facultative MBR integral sewage treatment equipment. The facultative MBR integral sewage treatment equipment comprises an anaerobic tank, a facultative tank and an MBR reaction tank, wherein the anaerobic tank, the facultative tank and the MBR reaction tank are communicated with one another in sequence; the anaerobic tank is internally provided with a water inlet pipe, a water allocating pipe communicated with the water inlet pipe and internal hanging biological filler; the facultative tank is filled with a spherical suspended carrier; anaerobic bacteria or facultative bacteria grow inside the spherical suspended carrier; aerobic bacteria grow outside the spherical suspended carrier; the MBR reaction tank is internally provided with an MBR film component. According to the facultative MBR integral sewage treatment equipment, in specific practice for curbing water body pollution, through the combination of a physical method, a chemical method and a biological method, a specific environment of a water body is taken into full account. Compared with the prior art, the facultative MBR integral sewage treatment equipment is only provided with the anaerobic tank, the facultative tank and the MBR tank, instead of an aerobic tank, therefore the technological procedure is simple, and the investment cost is lowered.

A soil-based disposal trench that has failed from a biomat slime is recovered by adding a facultative anaerobic bacteria to an effluent that flows into the disposal trench. The facultative anaerobic bacteria consumes the biomat slime and, in one embodiment, supplants a strictly aerobic bacteria in the soil that converts nitrites to nitrates. As a result, the facultative anaerobic bacteria interrupt the conventional process of nitrification, changing the nitrites to nitrogen gas.