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Aerobic type facultative autotrophic denitrifying bacteria and application thereof

A technology of autotrophic denitrification and bacteria, which is applied in the direction of bacteria, aerobic process treatment, chemical instruments and methods, etc., can solve the problems of increasing the application difficulty of anaerobic-autotrophic denitrification bacteria and increasing the application cost

Active Publication Date: 2019-03-26
HEILONGJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since anaerobic-autotrophic denitrifying bacteria are obligate anaerobic bacteria and need to provide additional electron donors (ie hydrogen or sulfide), these characteristics increase the difficulty of the application of anaerobic-autotrophic denitrifying bacteria and also increase the its application cost

Method used

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  • Aerobic type facultative autotrophic denitrifying bacteria and application thereof
  • Aerobic type facultative autotrophic denitrifying bacteria and application thereof
  • Aerobic type facultative autotrophic denitrifying bacteria and application thereof

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specific Embodiment approach 1

[0012] Specific implementation mode 1: In this implementation mode, an aerobic facultative autotrophic denitrifying bacterium is Pseudomonaskoreensis Y5-11, which is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is Beichen West Road 1, Chaoyang District, Beijing No. 3 Courtyard, the preservation date is October 29, 2018, and the preservation number is 16651.

[0013] The screening method of aerobic facultative autotrophic denitrifying bacteria Pseudomonas koreensis Y5-11 in this embodiment is as follows: put 100mL low-temperature (about 4°C) groundwater samples into a 4°C incubator for enrichment culture, and add 0.1g / L NaNO 3 ; After 7 to 10 days, the water sample is obviously turbid, indicating that there is microbial growth, and the water sample is transferred to the separated and purified solid medium; the main component of the medium is: NaNO 3 0.1~0.5g / L, MnSO 4 0.01~0.05g / L...

specific Embodiment approach 2

[0016] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the aerobic facultative autotrophic denitrifying bacteria Pseudomonas koreensis Y5-11 is Gram-negative, bacilli, 2-4 μm long, and 0.5-0.9 μm wide. μm, no spores, have flagella, and move by flagella, have capsules, form milky white colonies on LB medium, the colonies are irregularly round, and the surface is convex. Others are the same as the first embodiment.

specific Embodiment approach 3

[0017] Embodiment 3: In this embodiment, an aerobic facultative autotrophic denitrifying bacterium is applied to remove pollutants in groundwater.

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Abstract

The invention relates to aerobic type facultative autotrophic denitrifying bacteria and application thereof. The aerobic type facultative autotrophic denitrifying bacteria are Pseudomonas koreensis Y5-11 which is preserved in China general microbiological culture collection center, the preservation address is No.3, No.1 Beichen West Road, Chaoyang District, Beijing, the preservation date is October 29, 2018, and the preservation number is 16651. The strain of the aerobic facultative autotrophic denitrifying bacteria can be grown by using an organic carbon source, or CO2 can be used as a sole carbon source to reduce nitrate. The aerobic facultative autotrophic denitrifying bacteria are applied to the field of environmental microorganisms.

Description

technical field [0001] The invention relates to an aerobic facultative autotrophic denitrification bacterium and its application. Background technique [0002] Microbial denitrification refers to the process in which microorganisms reduce nitrate to nitrogen. This phenomenon is ubiquitous in nature (terrestrial, freshwater and marine systems). It is generally believed that denitrification can be divided into anoxic-heterotrophic denitrification and anaerobic-autotrophic denitrification. Anoxic-heterotrophic denitrification is a process in which organic matter (organic carbon) is used as an electron donor to reduce nitrate to generate nitrogen gas under anoxic conditions (dissolved oxygen is about 0.5mg / L). Anoxic-heterotrophic denitrification is characterized by the need for a large amount of organic carbon sources to maintain growth (C / N>10), and in the process of denitrification of sewage or slightly polluted groundwater, carbon sources are often insufficient, and gen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/02C02F3/34C12R1/38C02F101/16
CPCC12N1/02C12N1/20C02F3/02C02F3/34C02F2101/163C12R2001/38C12N1/205Y02W10/10
Inventor 张多英张彦龙白也雷虹曾伟民赵丹丹
Owner HEILONGJIANG UNIV
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