33 results about "Embryology" patented technology

Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with VSV-G protein, which is a surface glycoprotein of vesicular stomatitis virus (VSV) The present invention provides simian immunodeficiency virus vectors for gene transfer to primate ES cells. The method for gene transfer to primate ES cells using the vectors of the present invention is useful in, for example, research into embryology and disease, clinical applications, and experimental models for primates. The method is also useful in assaying and screening for genes and reagents able to enhance the specific differentiation of tissues or cells, and which are useful in preparing desired cells or tissues differentiated from ES cells.

The invention relates to an oryzias melastigma fertilized egg hatching method, and belongs to the field of aquaculture. According to the oryzias melastigma fertilized egg hatching method, the fertilized eggs are hatched in seawater with different salinity gradients, the physical and chemical conditions for hatching are the temperature being 26-28 DEG C, the humidity being 40-60%, the light intensity being 80-125[mu]mol.m-2.s-1, and the light-dark ratio being 14:10. The fertilized eggs with high cleanliness can be obtained, experimental operations such as embryology experiment observation and microinjection can be carried out conveniently, and the hatching rate of the fertilized eggs can be as high as 94.7% by using the oryzias melastigma fertilized egg hatching method. Moreover, hatched oryzias melastigma can develop, grow and reproduce normally after the long-term observation, and no abnormal phenomena are found.

The invention discloses a cell culture box for histogenetic experiments, comprising a box body, a box door and a culture placing rack. A box door is hingedly installed on one side of the box body through hinges, and the top of the box body and the An ultraviolet germicidal lamp and a heater are respectively fixedly installed at the bottom, a plurality of culture placement racks are installed inside the box body, a base is installed at the bottom of the box body, and the culture placement racks pass through the notch on one side of the box body Installed with the box, the culture placement rack includes a U-shaped placement slot and an end baffle installed on one end of the U-shaped placement slot, the U-shaped placement slot is fixedly installed with a cell culture dish, and one of the U-shaped placement slot is installed A sliding block is installed on the side, and the culture placing rack is slidably installed with the box body through the sliding block and the sliding rail on the box body. The invention has a compact structure, can realize the efficient culture of cells for histoembryology experiments, is less affected by external interference, has less pollution, is simple in operation, and is very flexible in use, and can improve the culture quality of the cells in the incubator.

The invention discloses a small interfering RNA (siRNA) inhibiting expression of a myostatin (MSTN) gene in chicken and application thereof. The invention provides an siRNA which acts on the MSTN gene, wherein the siRNA is a) a double-stranded RNA composed of the sequence 1 and the sequence 2 in a sequence table, b) a double-stranded RNA composed of the sequence 3 and the sequence 4 in the sequence table or c) a double-stranded RNA composed of the sequence 5 and the sequence 6 in the sequence table. The invention obtains the siRNA effectively inhibiting expression of the MSTN gene in chicken,real-time quantitative polymerase chain reaction (PCR) verifies that the siRNA effectively reduces expression of MSTN in molecular biology, and chicken with skeletal muscles obviously increased are successively obtained through microinjection test in embryology. The above results show that the siRNA provided by the invention can be used for breeding chicken, thus improving the meat yields of broilers, especially high quality chicken.

The invention discloses a siRNA capable of restraining chicken myostatin (MSTN) gene expression and application of the siRNA; small interfering RNA (ribonucleic acid) acted on myostatin gene is provided by the invention and is a) or b) or c) as follows: a) double-stranded RNA composed of a sequence 1 of a sequence table and a sequence 2 of the sequence table; b) double-stranded RNA composed of a sequence 3 and a sequence 4 of the sequence table; and c) double-stranded RNA composed of a sequence 5 and a sequence 6 of the sequence table; according to the invention, the small interfering RNA effectively capable of restraining chicken MSTN gene expression is obtained and is proved to effectively reduce expression of MSTN through real-time fluorescent quantitative PCR (polymerase chain reaction) on molecular biology; chicks with obviously enlarged skeletal muscle is successfully acquired through a micro-injection test on embryology; above-mentioned results show that the small interfering RNA provided by the invention can be used for breeding the chicks, so that chicken yield of the chicks specifically high-quality chicks is increased.

A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

The invention discloses a method for preparing a composite door panel. The operation steps are as follows: (1) cutting: decontamination treatment is performed on the raw materials, ingredients are prepared according to the requirements of the drawing, and the wooden frame, filler board and surface board are cut; (2) high temperature treatment : Carry out high-temperature treatment on the wooden frame, filler board, and surface board; (3) Thickness sanding; (4) Gluing and forming embryos; (5) Glue pressing: Send the slabs to the hot press, start the hot press, The temperature is controlled at 150°-170°, the compression time is 200s-300s, and the pressure is 4MPA-6MPA. After the hot pressing is completed, let it stand for 30-45min and remove the slab; (6) veneer the cooled slab Edge sealing, processing forming edge, processing assembly hole for assembly; (7) Spraying topcoat. The invention improves the preparation method, effectively prevents degumming and even warping of the composite board by controlling the moisture content of the composite board, and improves the appearance quality of the composite board.

The invention provides a device for extracting and delivering germ cells in the in-vivo experimental rat genital tract and belongs to the technical field of animal embryology. The device for extracting and delivering the germ cells in the in-vivo experimental rat genital tract consists of a moving suction ball (1), a suction and delivery tube (2), an operating bag (3), a computer data line (39) and a control computer (40) and is characterized in that the moving suction ball (1) is olive-shaped, the cross section of the moving suction ball (1) is oval, the longest diameter of the moving suction ball (1) ranges from 0.5 cm to 1 cm, and the shortest diameter of the moving suction ball (1) ranges from 0.05 cm to 0.3 cm. A ball top suction opening (4), a camera (28) and a flash lamp (29) are arranged at the front end. Two to five circles of forwards-moving tube feet (6) are arranged at the front half portion, four to eight ball waist suction openings (5) are arranged in the middle, two to five circles of receding tube feet (7) are arranged at the rear half portion, a ball tube connector (8) is arranged at the rear end and enables the moving suction ball (1) and the suction and delivery tube (2) to be connected together. The device for extracting and delivering the germ cells in the in-vivo experimental rat genital tract is simple in manufacture, good in operability, low in cost and obvious in effect.

The invention provides a method for efficiently extracting DNA of a fertilized egg genome of ascaris suum. The method comprises the following five steps: washing eggs with distilled water; pre-treating the eggs; digesting protease K; removing impurities; and eluting DNA with high-purity water. The extracted DNA of the fertilized egg genome of ascaris suum has stable quality and can be used for effectively performing downstream PCR amplification, and an important technical method is provided to studying of molecular epidemiology, genetic diversity, genetic structure, systemic embryology and phylogeography of ascaris suum. The method has an important meaning to molecular diagnosis, prevention and control on porcine ascariasis.

The present invention is providing a system based on artificial vision, and artificial intelligence that is capable of assisting the embryologist to select the best spermatozoa to be injected during an ICSI procedure, requiring the selection of a single sperm. It can identify the best spermatozoa from a sample, in real-time, based on their morphological and motility characteristics which are observed under the microscope at magnifications equal to or above 20×.

Is an automatic analysis of sequences of images produced by a digital camera attached to a microscope in real-time. It uses computer vision algorithms to automatically detect and track each of the sperms present on every image of a video and compute a number of features related to the morphological characteristics and motility parameters. The features of each sperm are processed and then evaluated using a mathematical model which determines the quality of each sperm and ranks them accordingly.

The invention discloses a live embryo surface zona pellucida tension detection device. The device comprises an inverted microscope, further comprises a pressure sensor which is fixedly connected witha first operating arm of the inverted microscope, an extrusion needle which is fixedly connected with the other end of the pressure sensor and is used for extruding an embryo, a fixed needle which isfixedly connected with a second operating arm of the inverted microscope, and a micromanipulation vessel which is positioned on an objective table of the inverted microscope, wherein the pressure sensor is electrically connected with the computer through a data line, and the pressure sensor transmits detected data to the computer for storage. The device is advantaged in that the pressure sensor isadditionally arranged on an existing inverted microscope, a camera on the inverted microscope is combined, collection and research of tension-related data of a transparent belt on a surface of a tested living embryo are facilitated, a separation blade is arranged in the micromanipulation vessel so that a supporting surface is provided for the living embryo conveniently, a zona pellucida on the surface of the living embryo is slightly deformed under the combined action of the extrusion needle, the fixed needle and the separation blade, the living embryo is not damaged, finally, a tension coefficient of the living embryo is calculated, and embryologists can judge activity of the living embryo by using data and common indexes for embryo judgment conveniently.

The present invention is directed to the seed-to-seed perpetuation of hybrid vigor and other traits through apomixis (asexual seed formation) in flowering plants (angiosperms). More particularly, to predictable methods for producing, from sexual or facultatively-apomictic plants, progeny plants that express an increased percentage of apomictic seed set or one or more elements of apomixis. This invention uses: plant cytoembryology procedures to identify and select a plant or group of plants that possess appropriate genetic variability for initiation times and durations of megasporogenesis (female meiosis), embryo sac formation, egg and central cell formation and maturation, fertilization, embryony and endosperm formation; plant breeding procedures to produce numerous and divergent genetically-recombined early to late generation progeny such that embryo sac formation preempts megasporogenesis and embryony preempts fertilization; and plant cyto-embryology or progeny test procedures to select segregant plants that express an increased frequency of one or more elements of apomixis.

The invention belongs to the technical field of embryology. The invention provides beef cattle reproductive embryo cryopreservation liquid and a freezing method. The cryopreservation liquid is prepared from the following components in parts by weight: 40 to 50 parts of an HPES buffer solution, 0.1 to 0.5 part of bovine serum albumin, 2 to 5 parts of sorbitol, 0.01 to 0.02 part of glutamine, 10 to15 parts of polyethyleneimine and 20 to 25 parts of cane sugar. The beef cattle reproductive embryo freezing method comprises the following steps: balancing beef cattle embryos to be frozen in a pre-balancing solution for 5-8 minutes, balancing the beef cattle embryos in the beef cattle reproductive embryo freezing preservation solution according to any one of claims 1-3 for 30-40 seconds, transferring the beef cattle reproductive embryo freezing preservation solution to a freezing carrier, and putting the freezing carrier into liquid nitrogen for freezing treatment. By means of the technicalscheme, the problem that in the prior art, the cryopreservation effect of cryopreservation liquid on cells is poor, and consequently the cryopreservation survival rate of the cells is low is solved.

The invention discloses an embryology virtual simulation teaching system fused with a VR game, the teaching system comprises a knowledge explanation module, an animation game practice module and a knowledge assessment module, the knowledge explanation module is used for clicking introduction and playing theoretical knowledge explaining embryology; the animation game practice module is used for constructing and displaying the whole process of sperm-egg combination in embryology in a VR animation game mode, and further understanding and learning embryology knowledge; and the knowledge assessment module is used for assessing theoretical knowledge of embryology in a time-limited manner. By fusing the VR game, the process of combining the sperms and the ova is constructed in a game mode, the whole process of combining the sperms and the ova is vividly displayed, teaching and science popularization application are better carried out, and the integration of academic teaching and the virtual technology is realized.

A method for directly amplifying the DNA of a single human roundworm egg without extraction, comprising four steps of washing the egg with distilled water, breaking the egg, PCR amplification and result detection. The invention can obtain PCR products with good stability and high amplification efficiency by optimizing the fragmentation method of eggs and the PCR amplification system, and can avoid the amplification failure caused by the loss of DNA in the extraction process. The amplified product obtained by the present invention can effectively perform downstream gene sequencing and STR scanning, and provide important technical methods for molecular epidemiology, genetic diversity, genetic structure, phylogenetics, phylogenetic geography and paleontological research of human roundworms , the invention is also of great significance to the molecular diagnosis, prevention and control of human ascariasis.

The invention discloses an application method of the sixth finger in histology and embryology experiment teaching. The sixth finger can be obtained through operation; after the fixation of a fixation solution of a certain concentration, the sixth finger is subjected to dehydration, hyalinization, waxdipping, embedding, slicing, dyeing and slice packaging in a certain manner; and finally, the sixth finger is used for observation of epithelial tissue, connective tissue, cartilage and bone and muscular tissue in histology and embryology experiment teaching, so that the problem that it is not easy to obtain a normal human tissue sample in the histology and embryology experiment teaching is solved, and effective utilization of experiment resources is expanded. Besides, the sixth finger is easily available, can be obtained from an operating room of the department of orthopaedics, can be obtained timely, and can be applied to the scientific research of human tissue engineering; the cell culture technique is adopted to separate epithelial cells, adipose cells and osteoblasts of the sixth finger, and thus the obtained cells are closer to real cell experiment; and the sixth finger is used for scientific experiment researches.