58 results about "Assisted fertilization" patented technology

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

This invention relates to the interdisciplinary field of assisted reproductive technology and artificial intelligence, specifically to a system and method for constructing an embryonic developmental trajectory map that integrates morphological features. By deeply integrating static morphological features with dynamic morphodynamic parameters, a more comprehensive panoramic map of embryonic development with richer information dimensions is constructed, overcoming the one-sidedness of single-dimensional assessment. Automated analysis of the map using an AI model reduces the influence of subjective human factors, enabling the discovery of subtle developmental patterns and potential differences that are difficult to discern with the human eye, significantly improving the accuracy of selecting high-quality embryos. The provided developmental trajectory map allows embryologists to intuitively examine the entire developmental process of the embryo and the performance of each key node, enhancing the interpretability and credibility of the assessment process. Through more scientific embryo selection, it is expected to improve embryo implantation and clinical pregnancy rates, and reduce multiple pregnancy and miscarriage rates.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

The invention discloses application of rutin hydrate in preventing and treating repeated embryo planting failure, and belongs to the technical field of assisted reproduction. It is found for the first time that CD36 is a key membrane protein for lipid transport and uptake of EECs, rutin hydrate competitively inhibits combination of CD36 and natural ligands of CD36, abnormal lipid excessive uptake is remarkably blocked, lipid accumulation and lipid toxicity damage in cells are effectively relieved, and the effect of inhibiting EECs lipid transport and uptake is achieved. And finally, the endometrial receptivity defect related to RIF is reversed, and the embryo adhesion and implantation capability is remarkably improved. Therefore, rutin hydrate provides a brand new strategy for developing targeted therapy drugs for RIF, especially for lipid metabolism disorder type endometrial receptivity badness, and has remarkable clinical application and transformation prospects.

This image is an information display image used for observing sperm in assisted reproductive technology. This image includes an object (information display section) that is displayed according to the position of the sperm and displays information about the sperm, and an object (display information selection section) for selecting information about the sperm to be displayed by the information display section. The information display section displays information about the state of the sperm, for example, using color or brightness. The display information selection section is an operation section that enables selection of information about the sperm to be displayed by the information display section. Image 1 showing the changed state is an image showing the state in which the information displayed by the information display section in the image diagram has changed over time, etc. Image 2 showing the changed state shows that it is also possible to prevent the information display section from being displayed by selecting the display information selection section.

This utility model discloses an improved semen DNA collection card for assisted reproductive technology, belonging to the field of medical supplies technology. It includes a folding card with an upper cover, a lower cover, and a bottom surface. The bottom surface is located between the upper and lower covers. The lower cover has a sampling filter paper surface, and a PVC diaphragm is provided in the middle of the sampling filter paper surface to divide it into a pre-processing semen collection area and a post-processing semen collection area. This utility model stores the patient's unprocessed semen in the pre-processing semen collection area and the processed semen in the post-processing semen collection area. The PVC diaphragm between the two areas prevents interference and cross-contamination between the two semen samples, facilitating DNA comparison before and after semen processing. The folding card also facilitates transportation and transfer, allowing for the storage of both pre- and post-processing semen within a single card, which is beneficial for clinical research.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The present invention relates to the identification and / or selection of sperm, and in particular a means for identifying and / or selecting the fittest sperm for use in assisted reproductive technologies.

The present application relates to the technical field of biological medicine, and more particularly to a culture solution, a kit and an application for in-vitro maturation of aged oocytes. Through in-vitro experiments, the present application adds exogenous stromal cell-derived factor 1, establishes a culture system for in-vitro maturation of aged oocytes, and reveals the molecular mechanism of stromal cell-derived factor 1 playing a role through autophagy, thereby providing a new solution for improving the fertility of aged women and improving the success rate of assisted reproductive technology, and having important clinical application value.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

This invention discloses a method for predicting the outcome of in vitro fertilization-embryo transfer (IVF-ET) based on static embryo images, used to distinguish between biochemical pregnancy and clinical pregnancy. It belongs to the field of medical artificial intelligence and assisted reproductive technology and is applicable to single embryo transfer (SET) and dual embryo transfer (DET) scenarios. The method includes: acquiring a static image of at least one embryo to be transferred; preprocessing and standardizing the embryo image, and then inputting it into a feature extraction model based on a convolutional neural network. The model uses DenseNet-169 as the backbone network and combines it with a Feature Pyramid Network (FPN) for multi-scale feature fusion. When the input is a dual embryo image, a Trophectoderm–Inner Cell Attention (TEIC-Attn) attention module is introduced to adaptively weight and fuse the features of the two embryos to characterize the contribution of different embryos to the final transfer outcome. When the input is a single embryo image, the prediction is completed based on the corresponding embryo features. The model ultimately outputs a probability prediction result of whether the IVF treatment outcome is a biochemical pregnancy or a clinical pregnancy. Furthermore, this invention combines a gradient-based class activation mapping method to generate interpretable visual heatmaps, highlighting key morphological regions closely related to embryo transfer outcomes, such as the inner cell mass and trophectoderm, thereby providing clinicians and patients with intuitive and reliable decision-making support. This invention enables unified prediction and interpretation of single and double embryo transfer outcomes without relying on time-series data, improving the accuracy and interpretability of in-vitro fertilization treatment decisions and demonstrating promising clinical application prospects.

This invention discloses a microfluidic chip for three-stage non-destructive sperm screening and its usage method. The chip includes a substrate, a sample inlet channel layer, a sample chamber layer, a recovery chamber layer, and a screening filter membrane. The sample inlet channel layer has an inlet channel and an overflow channel. The sample chamber layer has an annular sample chamber, an inlet orifice, and an overflow orifice. The recovery chamber layer is divided into an outer recovery chamber and an inner recovery chamber by a dike. An inducing agent patch is located in the center of the inner recovery chamber. The screening filter membrane is sandwiched between the sample chamber layer and the recovery chamber layer. In use, after injecting a sperm sample, initial motility screening is achieved through the screening filter membrane. Secondary chemotactic screening is completed through an inducing agent concentration gradient. Tertiary adaptive screening for swimming direction is completed using the chemotactic channel. Finally, high-quality sperm are obtained from the inner recovery chamber. This invention achieves multi-parameter graded screening, effectively avoiding sperm damage, improving sperm recovery quality and screening efficiency, and is applicable to various assisted reproductive technologies, helping to improve conception success rates.

This invention relates to the fields of biomedical engineering and embryo quality assessment, and particularly to a biomarker for assessing the quality of IVM-derived embryos and its application. The biomarker includes time point parameters t2 and t9 of the IVM-derived embryos, and time interval parameters S2, t9–t7, t9–t8, or tSB–t9. A method for assessing the quality of IVM-derived embryos based on the aforementioned biomarker has been established. This method combines non-invasive temporal imaging technology with morphodynamic multi-parameter analysis, providing a reliable tool for assessing the blastocyst formation potential and chromosomal euploidy of IVM-derived embryos in clinical practice, thus potentially expanding the application of IVM technology in assisted reproductive technology.

The present application relates to the field of biomedical technology, and more particularly to a prediction model for embryo quality outcome of women of childbearing age undergoing assisted reproductive technology; the present application is based on clinical and laboratory data of women of childbearing age undergoing assisted reproductive technology, uses light GBM multi-factor analysis to screen the key factors of clinical core parameters affecting women of childbearing age undergoing assisted reproductive technology, and performs spear correlation analysis with each clinical core parameter to obtain core factors affecting embryo quality outcome of women of childbearing age undergoing assisted reproductive technology, and to construct a model capable of accurately predicting embryo quality outcome, which can accurately predict the positive influencing factors of embryo quality, effectively reduce the psychological burden of a part of ART pregnant patients, so as to make clinical intervention more targeted, more conducive to the rational allocation of clinical resources, and suitable for large-scale popularization and application.

Disclosed are methods and products for improving the quality of sperm, methods for treating subjects to improve sperm quality or improve fertility, and use of sperm with improved quality in assisted reproductive technologies, including exposing the sperm to BGP-15 and / or a derivative thereof, such as BGP-15, propanolol, bimoclomol, arimoclomal, NG94, iroxanadine, and / or a pharmaceutically acceptable derivative, prodrug, solvate, salt, tautomer, stereoisomer, and / or racemate thereof.

The present disclosure relates to a method of controlling an incubator including controlling an environmental condition of an incubation chamber for a first time period, wherein a pH value of media surrounding a biological specimen in a culture dish in the incubation chamber is affected by the environmental condition. The method includes controlling the environmental condition of the incubation chamber for a second time period, wherein the pH value of media surrounding the biological specimen in the culture dish during the second time period is different than the pH value during the first time period.

The invention belongs to the technical field of biology, and particularly relates to a method for improving in-vitro mature oocyte telomere length and maturation quality and application of the in-vitro mature oocyte telomere length and the maturation quality. And daphnetin. According to the preparation disclosed by the invention, at least one of the Sema3 type protein and the daphnetin is added, so that the telomere length of an oocyte and an in-vitro embryo is effectively maintained, repaired or prolonged, the maturation quality of the in-vitro oocyte and the development potential and development efficiency of the in-vitro embryo are further remarkably improved, and further, the development potential and development efficiency of the in-vitro embryo are further improved. The application provides a more effective new strategy for in-vitro embryo production of livestock, animal genetic improvement, assisted reproduction technology and the like, and the application prospect is wide.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a biological response type memristor for in-vitro embryo monitoring and application of the biological response type memristor. The memristor comprises a substrate, a bottom electrode, a dielectric layer and a top electrode which are sequentially arranged from bottom to top, the dielectric layer is a double-layer functional layer composed of an Nb2O5 thin film and a barium titanate thin film, the barium titanate thin film is located on the upper layer of the bottom electrode, and the Nb2O5 thin film is located on the upper layer of the barium titanate thin film. The memristor realizes response to biological information by monitoring metabolic byproducts and oxidative stress markers in an embryo in-vitro culture solution in real time, realizes operation by inducing redistribution of surface charges of an interface through ion adsorption, and shows a resistance state transformation characteristic. Besides, the system breaks through the limitation of traditional microscope visual evaluation, realizes direct, sensitive and quantitative electric signal reading of the embryo metabolism state and the oxidative stress level, and solves the problem of embryo development potential evaluation caused by the increase of aneuploid rate in old lying-in women and recurrent implantation failure groups.

A method of determining fusogenic potential of sperm is provided. The method comprising: (a) contacting a biological sample comprising sperm with somatic cells expressing ectopic JUNO under conditions which allow binding of the sperm to the JUNO; and subsequently (b) detecting multinucleation in the somatic cells, wherein a level of the multinucleation is indicative of the fusogenic potential of the sperm.

The invention discloses a culture solution for promoting in-vitro maturation of bovine oocytes and application of the culture solution, and belongs to the technical field of assisted reproduction. The invention provides the culture solution which is composed of brand new components and is used for promoting in-vitro maturation of the bovine oocytes, support is provided for maturation of the oocytes from multiple aspects of carrier transportation, energy metabolism regulation, signal transduction, extracellular environment and the like, the in-vitro maturation quality of the bovine oocytes can be effectively promoted, and the survival rate of the bovine oocytes is increased. The quality and consistency of nucleus maturation and cytoplasm maturation of oocytes are obviously improved; the cultured mature oocytes are used for in-vitro fertilization, so that the fertilization proportion and the blastocyst hatching rate can be remarkably improved; the provided culture solution is appropriate in component number, clear in proportion, simple and easy to operate in preparation method, short in time consumption, good in repeatability and suitable for batch production, and has a good application prospect in maturation culture of bovine in-vitro oocytes.

The application discloses a culture medium for improving in-vitro aging of oocytes after ovulation and application thereof. The culture medium is prepared by adding 0.1-30 muM of astragaloside IV into an oocyte culture solution. The culture medium is safe, non-toxic and free of side effects, can reduce the oocyte fragmentation rate, active oxygen level and abnormal spindle rate of the oocytes after 24 hours of in-vitro culture, can significantly improve the mitochondrial membrane potential, in-vitro sperm binding capacity, in-vitro fertilization pronucleus rate and cleavage rate of the mature oocytes after 24 hours of in-vitro culture, and can improve the quality of the late embryos. The application of the culture medium provides favorable support for assisted reproductive technology, and has a good application prospect.

The invention belongs to the field of drug sustained release preparations, and provides an ovulation inducing preparation for improving fertility and a preparation method thereof. The core-shell structure microsphere design is adopted, leuprorelin acetate is entrapped in the core of the polylactic acid microsphere, and covalently anchored amido bond connection is formed through an end group activated polylactic acid NHS ester intermediate and a chitosan-sodium tripolyphosphate ion network shell layer, so that the high stability of the shell layer is realized; by optimizing the compounding use of the internal phase stabilizer and the freeze-drying protective agent, the particle size distribution D50 of the microspheres is controlled to be 10-60 microns, the thickness of the shell layer is controlled to be 0.05-5 microns, and high encapsulation efficiency, good drug slow release performance and excellent storage stability are realized. The technical problems that in an existing peptide medicine core-shell microsphere system, a shell layer is prone to falling off, the medicine is suddenly released, and the stability is poor after freeze-drying are solved, and the peptide medicine core-shell microsphere has the advantages of being high in encapsulation efficiency, controllable in release and good in biocompatibility and has wide application value in the technical field of assisted reproduction.

The utility model relates to the field of assisted reproductive technology equipment discloses a kind of bovine in vitro embryo culture environment simulation device, including device body assembly, the device body assembly includes simulation device body, the outer surface of the simulation device body is rotatably connected with turnover observation window, the bottom of the simulation device body is fixedly connected with buffer rod by bottom mounting seat.The utility model is through the series connection effect of first spring damper, buffer spring and second spring damper, device forms gradient shock-absorbing network in three-dimensional space, horizontal direction impact is preferentially absorbed by first spring damper and converted into heat energy dissipation, vertical load is guided to spiral spring by buffer rod and carried out flexible support, extend the action cycle to reduce peak acceleration, moving block linkage mechanism cooperates second damper to realize the vector decomposition of oblique disturbance, finally reduce the vibration amplitude at culture dish, provide nearly stationary growth interface for embryo cell division.

This disclosure features methods and compositions for the in vitro maturation of oocytes. In particular, this disclosure features methods and compositions relating to the manipulation of multiple ovarian supporting cells (OSCs) to promote the maturation of oocytes in a culture medium. Such methods and compositions are particularly useful in assisted reproductive technology (ART) procedures.

The invention discloses a porcine epididymal cell-derived extracellular vesicle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. By optimizing the preparation method of the extracellular vesicles, the extracellular vesicles with high purity and standard shape are successfully obtained. Furthermore, by virtue of a proteomics technology, the composition characteristics of rich key functional proteins related to oxidation resistance, movement regulation and fertilization are systematically clarified for the first time. Functional experiments prove that the vesicle can be effectively attached to sperms, the sperm motility is remarkably improved under the low-temperature preservation condition, the oxidation resistance is enhanced, membrane damage, mitochondrial dysfunction and DNA damage are relieved, and the specific protection effect on the sperms is shown. The invention provides a reliable material basis and theoretical basis for developing a novel sperm preservative and an assisted reproduction technology based on extracellular vesicles.

The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and application thereof in preparation of a medicine for improving and / or treating male infertility. The traditional Chinese medicine composition is prepared from Fructus Malli, Fructus Equiseti, Rhiocarpae, Radix Rehmanniae Preparatae, Rubus chingii Hu, Fructus Carpesii, Rhizoma Polygonati, Morus, Fructus Lycii, Semen Marsdeniae, Fructus Schisandrae Chinensis, Semen Plantaginis, Fructus Rosae Laevigatae, Radix Glycyrrhizae Preparatae. The traditional Chinese medicine composition is supplemented with multiple medicinal materials on the basis of a traditional prescription, forms a comprehensive conditioning scheme considering multiple viscera and multiple causes, a multi-dimensional syndrome differentiation and treatment strategy, and is beneficial to the treatment purpose of treating both the symptoms and the root cause, can more effectively cope with the multiple pathological changes of oligospermia and asthenospermia of male infertility, and improve the overall treatment effect. The traditional Chinese medicine composition is applied in cooperation with an assisted reproductive technology, and the effect is remarkable.

Disclosed in the present invention are a composition and method for increasing the number and proportion of inner cell mass cells of an in vitro embryo and use. According to the present invention, in view of the problems of in vitro fertilized embryos such as low blastocyst rate during early development, low inner cell mass cell number in the blastocyst stage, low proportion of the inner cell mass cells, and poor subsequent embryonic development potential, by adding one or two of regulators folic acid and laxiflorin B into the culture medium for early embryo in vitro culturing, the blastocyst rate, the number and proportion of the inner cell mass cells in the blastocyst, the survival rate of blastocysts after extended culturing, and the diameter of blastocysts are significantly improved, effectively improving the developmental potential of in vitro embryos. In addition, the present invention also effectively improves the ratio of in vitro fertilized embryos that can be subsequently transplanted. The present invention is low in cost, safe, and efficient, provides a new strategy for in vitro embryo production of livestock and development of human-assisted reproductive technology, and exhibits broad application prospects.

The invention provides a fertilization result prediction method and device for in-vitro fertilization and electronic equipment, and relates to the technical field of assisted reproduction. Comprising the following steps: performing oocytoplasm segmentation processing on each frame of collected image, and determining an oocytoplasm image area of each frame of image; dividing the oocytoplasm image region of each frame of image to obtain a plurality of oocytoplasm proton image regions of each frame of image; according to the information of each pixel point in each oocyte proton image region of each frame of image and a preset pixel coordinate offset range, generating average displacement information and average speed information of a particle swarm under each oocyte proton image region of each frame of image, and further generating various motion time sequence feature data corresponding to the target oocyte; and predicting a fertilization result corresponding to the target oocyte according to the multiple motion time sequence characteristic data corresponding to the target oocyte. The method improves the prediction accuracy of the fertilization result, and provides a judgment basis for early fertilization remediation of an embryologist.