32 results about "Granulosa cell" patented technology

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The invention belongs to the technical field of biological medicine, and particularly relates to a reagent for improving ovarian development and a preparation method and application thereof. The reagent for improving ovarian development is prepared by mixing the following raw materials in parts by weight: 20-30 parts of N-acetylcysteine; 5 to 10 parts of tranexamic acid; 3 to 8 parts of creatine; and 3000 parts of water. According to the reagent, the mRNA expression of PKD1P6 and follicle stimulating hormone receptors (FSHR) in ovarian tissues is up-regulated, the activity and functions of cumulus granular cells are enhanced, and apoptosis of the cumulus granular cells is inhibited, so that the effects of effectively promoting follicle development and inhibiting the apoptosis of the cumulus granular cells are achieved.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The invention discloses a method for promoting ovulation of sexual maturity zebrafish by using Gap27, and relates to the technical field of artificial breeding of fish, a gap connecting protein inhibitor Gap27 is applied to ovulation induction of zebrafish, an intercellular communication network in follicles is accurately intervened, the problem of asynchronous ovulation is solved, and in the final stage of maturation of oocytes, ovulation of sexual maturity zebrafish is promoted. Close substance and information exchange is carried out among follicular granule cells through a gap connecting channel formed by connecting protein Cx43, and Gap27 is used as a specific peptide fragment, can simulate an extracellular ring structure of Cx43, and competitively binds with Cx43 on an adjacent cell membrane, so that formation and functions of the gap connecting channel are physically blocked, and the follicular granule cells are separated from each other. When ovarian tissues of sexually mature female fish are exposed in a Gap27 solution, the transmission of inhibitory signals from granular cells to oocytes can be effectively interrupted, the rupture of nuclear membranes of the cells is promoted, and the final maturation process is completed.

The present application relates to the technical field of computer vision, and particularly relates to an oocyte maturity analysis method, device and computer storage medium, the analysis method comprises the following steps: S1, classifying oocyte images into oocyte images with granulosa cells removed and oocyte images without granulosa cells removed; further classifying into mature and immature; S2, data augmentation is performed on the immature; S3, the oocyte images are segmented into non-overlapping image blocks, through four-stage processing, the feature maps of each image block in multiple dimensions are obtained; the weight scores of the feature maps in different dimensions are calculated in the dimension direction; the probability value of the category to which the image to be predicted belongs is output through a full connection layer, and the analysis of oocyte maturity is completed; the method of the present application has higher recognition preparation rate, faster speed, stronger device mobility, and has a significant effect on recognizing oocyte images before granulosa cells are removed.

The invention relates to an effect of an REV-ERBalpha agonist SR9009 in the aspect of delaying ovarian senescence, and aims at the problem of fertility decline caused by cliff-type decline of ovarian functions of women over 35 years old, the SR9009 is applied to activate an REV-ERBalpha circadian rhythm signal channel in ovarian tissues, inhibit excessive autophagy of granular cells and maintain a primordial follicle dormancy state, so that the agonist SR9009 can be used for delaying ovarian senescence. Therefore, the original follicle depletion speed is delayed, follicle storage is maintained, and the ovary reproductive function cycle is prolonged. The effect is verified by a mammal model: REV-ERBalpha protein expression in an ovary of an aging individual is reduced, rhythmicity expression is disordered, autophagy of granulosa cells can be effectively inhibited in the ovary of the aging individual intervened by the SR9009, and the number of residual primordial follicles and the number of total follicles are obviously greater than those of an untreated control group. The invention provides an innovative treatment strategy of targeted rhythm regulation and control for clinical intervention of ovarian aging.

The present application relates to a kind of two-dimensional metal polyphenol complex nanomaterials that can be enriched in ovary, preparation method and application thereof, the nanomaterial can be enriched in ovary, including two-dimensional metal polyphenol complex as substrate, and follicle-stimulating hormone polypeptide grafted to the surface of the substrate;Wherein, the surface of the two-dimensional metal polyphenol complex grafts-COOH functional group and forms 2D MPC-COOH intermediate, the follicle-stimulating hormone polypeptide is covalently grafted with the-COOH on the surface of 2D MPC-COOH intermediate and obtains the two-dimensional metal polyphenol complex nanomaterial.The technical effect of the present application is that the preparation method of nanomaterial is simple, and nanoparticle dispersibility is good, and biosafety is high, and nanomaterial can physically absorb BRD7 target protein through its special structure, block BRD7 into cell nucleus, and then realize the safe down-regulation of KAT7 path and the effective reversal of granulosa cell stress aging.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.

The invention discloses a multi-behavior recommendation method for cross-behavior comparative learning based on particle balls, and the method comprises the steps: obtaining a user set, an article set and user-article interaction data under various behavior types, and constructing a user-article diagram corresponding to each behavior; obtaining behavior specificity embedding under each behavior based on the user-article diagram; respectively constructing a particle ball set for the user and article embedding under each behavior; constructing an intra-behavior particle ball incidence matrix and an inter-behavior particle ball overlapping matrix; inputting the enhanced embedding representation into a target perception noise suppression encoder, distinguishing beneficial signals and noise in auxiliary behaviors based on a graph attention mechanism, and generating refined behavior embedding; and calculating a matching score between the user and the article based on the final representation to realize recommendation prediction of the target behavior. According to the method, a user-article interaction embedding space under each behavior is divided into a plurality of local particle balls with consistent semantics, and topological structures in the behaviors and cross-behavior high-order association are captured.

The application relates to a method for establishing an early ovarian failure rat model, which comprises the following steps: taking healthy rats, and performing gavage on the rats according to a dosage of 10-20 mg / Kg of a phytostilbene preparation, wherein the dosage is the ratio of phytostilbene to the weight of the rats, and obtaining the early ovarian failure rat model after four days of administration. The early ovarian failure rat model is obtained by performing gavage on the rats after the phytostilbene preparation, the model is simple to establish, and the model establishment time is short. Through ovarian pathological observation, it can be seen that the simulated animal model has obvious ovary tissue damage, follicular granulosa cell destruction and apoptosis, and follicle atresia and other symptoms. The obtained model has good stability and repeatability. The phytostilbene is used to successfully establish the early ovarian failure rat model, lays a good foundation for establishing a stable early ovarian failure model for basic research, and provides a reliable method for the construction of the early ovarian failure model.

This invention belongs to the field of biomedical technology, specifically relating to the application of a composition in the preparation of a drug for the prevention and treatment of polycystic ovary syndrome (PCOS). The composition comprises bovine spleen peptide and adenosine; the mass ratio of bovine spleen peptide to adenosine is 1:(1-2.5). Bovine spleen peptide, as a bioactive polypeptide, significantly improves insulin resistance associated with PCOS by targeting and activating the PI3K-Akt signaling pathway and promoting insulin receptor substrate phosphorylation. Adenosine, as a natural bioactive substance, significantly reduces serum luteinizing hormone and testosterone levels in rats and increases follicle-stimulating hormone levels, thereby effectively alleviating hyperandrogenism symptoms such as hirsutism and acne in PCOS. Furthermore, this composition can significantly improve polycystic pathological changes in the ovarian tissue of model rats, reduce the number of cystic dilated follicles, restore the granulosa cell layer structure, alleviate ovarian stromal hyperplasia, and promote normal follicular development.

The invention belongs to the technical field of biology, and particularly relates to a preparation for in-vitro maturation of oocytes and application of the preparation. According to the preparation for in-vitro maturation of the oocytes, a new choice which is stable, efficient, convenient, low in cost and safe is provided for in-vitro maturation culture of the oocytes by innovatively adding the cell lysis solution of the granular cells, the preparation is simple and convenient to use, and the preparation can be used for improving the maturation quality of the oocytes / the development potential of embryos and improving the survival rate of the oocytes. The batch-to-batch difference and the pollution risk are remarkably reduced, the repeatability and the result reliability of experiment and clinical application are ensured, and the application prospect is wide.

The invention discloses an in-vitro rapid detection method for ovarian repair activity of mesenchymal stem cell source exosomes. Specifically, the invention provides a KGN granular cell damage model based on 4-hydroxycyclophosphamide (4-hydroxycyclophosphamide, 4HCy) induction, and the model can be used for constructing an exosome function quality control system which is rapid, quantifiable, dose-dependent, capable of defining a threshold value and suitable for batch quality control. And a unified standard is provided for the processes of preparation, identification, function detection, quality control and release of the extracellular vesicles.

The invention discloses an application of an exosome loaded with ENO1 protein in preparation of a product for treating female fertility decline. The method comprises the following steps: transfecting a lentivirus overexpression vector containing an ENO1 gene into a 293T cell, culturing, collecting a cell supernatant, and separating to obtain the exosome loaded with the ENO1 protein. Experimental results show that after the exosome delivers the protein ENO1, the number of primordial follicles, secondary follicles and sinus follicles can be remarkably increased, and the state of low ovarian function is improved; meanwhile, the mitochondrial function of granular cells is enhanced, and the energy metabolism level is promoted. A further research shows that the ENO1 can be specifically combined with mitochondrial outer membrane protein VDAC1, the mitochondrial membrane potential is stabilized, and the mitochondrial structure integrity of the oocyte is recovered, so that the energy metabolism in the oocyte is improved. Therefore, the invention provides a new treatment strategy for the fertility decline of the elderly women, and can be widely used for treating the fertility decline of the elderly women.

A novel medical use of ingenol HEP14, a dosage form of an HEP14-based drug, and a preparation method for the dosage form. The dosage form of the HEP14-based drug is an HEP14 / PLGA microsphere sustained-release dosage form formed by encapsulating the HEP14-based drug with a polylactic acid-glycolic acid copolymer, and has the functions of high HEP14 drug loading rate, high encapsulation rate, and long-acting HEP14 sustained-release. It has been found for the first time that HEP14 can promote production and autophagy of mitochondria in cells and enhance the function of mitochondria in cells, promote the secretion of antioxidant enzymes and remove reactive oxygen species, promote the growth and viability of ovarian follicle granulosa cells and restore the growth and viability of aging granules, promote the secretion of angiogenic factors, reduce the fibrosis of ovarian tissues, promote the angiogenesis of ovarian tissues, and promote the regeneration of aged ovarian follicles and ovarian endocrine functions, and thus has a good effect of treating and / or preventing related diseases caused by mitochondrial dysfunction, especially the effect of treating age-related ovarian decline.

The invention relates to the technical field of genetic engineering, in particular to a gene for regulating chicken follicular granular cell proliferation and hormone synthesis and application thereof. The invention provides application of an IGFBP1 gene in regulation and control of chicken follicular granular cell proliferation and hormone synthesis. The amino acid sequence of the IGFBP1 gene is as shown in SEQ ID NO. 2. The technology can shorten the breeding period of new varieties of high-quality laying hens, has a wide application prospect, can assist in coping with related breeding challenges, improves breeding economic benefits, and provides support for innovation of a poultry genetic breeding technology.

This invention discloses a mixed cell preparation and its application in the preparation of drugs for treating premature ovarian failure. The mixed cell preparation comprises granulosa cells and mesenchymal stem cells, mixed in a certain ratio (preferably 1:1). Granulosa cells are obtained by inducing differentiation from mesenchymal stem cells and express granulosa cell-specific markers (FOXL2, FSHR, etc.), participating in follicular structure reconstruction; mesenchymal stem cells can improve the ovarian microenvironment, inhibit inflammation, and resist fibrosis, providing a suitable "soil" for the survival of granulosa cells. In vitro experiments are expected to show that mixed culture can significantly increase estradiol secretion levels and cell survival rate. Animal models of premature ovarian failure are expected to show that mixed cell therapy can significantly restore the estrous cycle, improve hormone levels, increase the number of follicles at all stages, and improve pregnancy rates, with effects significantly superior to single-cell therapy. This invention utilizes the synergistic effect of the two cell types to achieve dual repair of the "seed" and the "soil," providing a novel treatment strategy for premature ovarian failure.

The invention discloses a method for regulating and controlling pig follicular granule cell proliferation in a heat stress state. The method is realized by regulating and controlling the expression of miR-376a-5p in pig follicular granule cells in the heat stress state. According to the method, the expression of miR-376a-5p in the porcine follicular granular cells influenced by heat stress is regulated and controlled, specifically, in a heat stress state, the proliferation of the porcine follicular granular cells can be inhibited and the apoptosis of the porcine follicular granular cells can be promoted by promoting the expression of miR-376a-5p; by inhibiting the expression of the miR-376a-5p, the proliferation of the porcine follicular granule cells can be promoted and the apoptosis of the porcine follicular granule cells can be inhibited. Therefore, the proliferation and apoptosis of the pig follicular granule cells in the heat stress state can be effectively regulated and controlled. Granular cells are important components of follicle structures and functions, and play a core regulation role in follicle development and atresia processes. Therefore, by regulating and controlling proliferation and apoptosis of the pig follicle granule cells in the heat stress state, the growth state of follicles in the heat stress state can be further regulated and controlled, and a new method and thought are further provided for relieving sow reproductive disorder caused by heat stress.

The application aims to provide a new application of an iron death inhibitor, Liproxstatin-1. The application studies the iron death phenomenon in the process of follicle atresia of laying poultry, finds that the iron death inhibitor Liproxstatin-1 can rescue the follicular granulosa cell death induced by tamoxifen (TMX), can inhibit the oxidative stress level and intracellular iron ion overload caused by TTMX treatment, can effectively inhibit the follicular iron death phenomenon induced by TTMX, and finally can restore the egg production function of livestock and poultry, and has the development into a drug for treating follicle atresia and used for treating follicle atresia of livestock and poultry.

The invention discloses a GV-stage granulosa cell extraction method, which is characterized in that the number of granulosa cells is diluted to 4-6 * 10 < 5 > / L in the extraction process, the extracted cells can be utilized to the maximum extent, PSB is used for flushing in the extraction process, and high-glucose DMEM containing 0.8-1.2% of double antibodies and 8-12% of FBS is used, so that adherent culture of the granulosa cells is neater and cleaner, and the GV-stage granulosa cells are obtained. And adherence and growth of GV-stage granular cells are facilitated.

The application relates to an ovary state determination method and device, computer equipment, a storage medium and a computer program product. The method comprises the following steps: acquiring a target slice image of an ovary staining slice, and at least one target follicle meeting a follicle state analysis condition existing in the target slice image; identifying a first target region where the target follicle is located and a second target region where a follicle membrane layer is located from the target slice image; identifying a third target region where a wall layer granulosa cell layer is located from the first target region; determining the state of the target follicle based on the area size ratio of the second target region and the third target region, and obtaining a target follicle state determination result; and determining the state of the ovary according to the target follicle state determination result, and obtaining an ovary state determination result. The method can accurately evaluate the state of the ovary.

Disclosed are methods for increasing the fertilization rate of an oocyte in a granulosa cell-oocyte complex, and / or the success rate of in-vitro fertilization by using mitochondrial therapies. Also disclosed are methods of identifying compounds for fertility treatment.

The application discloses application of oleic acid in preparation of a medicine or a dietary supplement for delaying ovarian aging, and belongs to the field of biological medicine and reproductive medicine. The application discloses that the content of oleic acid is significantly reduced in the process of ovarian aging, and that the lack of oleic acid inhibits SIRT1-mediated peroxisome proliferator-activated receptor gamma deacetylation, causes the nuclear translocation of the peroxisome proliferator-activated receptor gamma to be blocked, and further activates the pyroptosis of granulosa cells mediated by NLRP3 inflammasome. Dietary supplementation of oleic acid can effectively inhibit the pyroptosis pathway, restore follicle reserve, improve the mitochondrial function and the level of reactive oxygen species of oocytes, and increase the number of natural mating litters. The application provides a nutrition intervention strategy with a clear mechanism for delaying female reproductive aging.

The application provides a long-chain non-coding RNA MSTRG.5970.28 and application thereof, a product and a method for goose ovary development, relates to the technical field of biology, and provides a new long-chain non-coding RNA MSTRG.5970.28; the lncRNA MSTRG.5970.28 is related to the proliferation and apoptosis of goose follicular granulosa cells; by regulating the expression of the lncRNA MSTRG.5970.28, the related miRNA and gene of the proliferation and apoptosis of the follicular granulosa cells can be correspondingly regulated. By applying the lncRNA MSTRG.5970.28 and the corresponding product, the research on the proliferation and apoptosis of the follicular granulosa cells and the physiological and biochemical functions can be effectively guaranteed. In addition, by regulating the proliferation and apoptosis of the follicular granulosa cells through the lncRNA MSTRG.5970.28, the population reproductive capacity can be improved while the excellent characteristics of the goose germplasm resources are maintained, so that the industrialization development of geese is promoted, and the economic benefits are improved.

The invention belongs to the technical field of poultry breeding, and relates to novel application of circFAM172A and related reagents in chicken follicle development, in particular to application of a reagent for detecting circFAM172A in preparation of chicken follicle development evaluation products and application of a reagent for reducing or inhibiting circFAM172A expression or a reagent for promoting circFAM172A adsorption in preparation of chicken follicle development promotion products. Research finds that circFAM172A can promote autophagy and apoptosis of chicken follicular granulosa cells, and circFAM172A can mediate apoptosis of the chicken granulosa cells by regulating and controlling autophagy of the cells, so that autophagy and apoptosis of the chicken follicular granulosa cells can be reversely regulated by reducing or inhibiting expression of circFAM172A. The invention discloses an important regulation effect of circFAM172A in follicle development, and provides a new theoretical basis and technical support for chicken reproductive performance and development of corresponding intervention strategies.

The invention relates to the technical field of cell culture, and discloses a reagent composition and method for in-vitro maturation three-dimensional culture of human oocytes, and the reagent composition comprises a three-dimensional hydrogel scaffold material formed by compounding sodium alginate, hyaluronic acid and collagen; the invention also relates to a culture medium containing a specific bioactive additive, wherein the additive comprises recombinant human anti-mullerian hormone, recombinant human follicle stimulating hormone and recombinant human epidermal growth factor with specific concentrations. The method comprises the following steps: mixing to-be-mature human oocytes and granular cells, embedding the mixture in the three-dimensional hydrogel scaffold to form cell hydrogel microspheres, and culturing the cell hydrogel microspheres in the culture medium. According to the method, the bionic three-dimensional microenvironment is constructed, and a specific factor combination is utilized to synergistically regulate and control ripening synchronization of nucleoplasm, so that the problem of low maturation quality of oocytes in traditional culture is solved, and the maturation rate, the fertilization rate and the blastocyst formation rate of the oocytes are remarkably improved.