174 results about "Water buffalo" patented technology

The invention discloses a low-temperature ultrahigh-pressure continuous sterilization method for liquid milk, relates to liquid milk, and particularly relates to a method for sterilizing the liquid milk by adopting ultrahigh-pressure water jet. The method is characterized in that the liquid milk is subjected to the ultrahigh-pressure water jet treatment in a nozzle of coaxial equivalent clash type or collision type at the temperature of between 4 and 60 DEG C under the pressure of 50 to 400MPa; and the liquid milk overcomes the defects that the pasteurized milk has short shelf life and the instantaneous ultrahigh-temperature sterilized milk has large loss of nutrient components, and can preserve the nutrition, prolong the shelf life, save time and labor and reduce the energy consumption. The method is applied to sterilization of cow milk, yak milk, water buffalo milk and goat milk, and the sterilized milk is expected to become a new variety of the liquid milk, namely ultrahigh-pressure milk.

The invention belongs to the technical field of animal feedstuff preparation, and specifically relates to a buffalo calf starter feed. According to the invention, corn, wheat bran, soybean meal, rice bran, brown sugar, stone powder, an anti-weaning-stress protective agent (chitosan), vitamins and a trace element premix are adopted as raw materials, and the raw materials are mixed according to a formulated weight ratio, such that the feed is obtained. The starter feed is suitable for buffalo calve unweaned or two weeks after weaning. The feed has comprehensive nutrients. With the feed, buffalo calf rumen development can be effectively promoted, buffalo calf intake of non-dairy feed can be increased, buffalo calf growth can be promoted, buffalo calf early weaning can be realized, adverse reaction of weaning stress to buffalo calves can be reduced, and a basis is provided for good production potential of adult buffalos.

The invention belongs to an animal feed technology field, concretely a milk replacer for river type and marsh type dairy hybrid hybrid buffalo calves. The milk replacer uses high protein whey powder, condensed whey protein, whole milk powder, skimmed milk powder, soybean oil, colza oil, butter, salt, emulsifier, methionine, lysine, calcium hydrogen phosphate, pre-mixture of vitamin and trace elements, anti-weaning stress protective agent chitosan as raw materials. According to a weight ratio of a raw material formula, the milk replacer is prepared with water to be a liquid in a certain proportion for feeding. The milk replacer of the invention contains various nutrients required for hybrid buffalo calf growth, which can replace buffalo milk to feed a buffalo calf, saves buffalo milk expenditure, and reduces calf breeding cost. Simultaneously, the milk replacer further contains an anti-weaning stress protective agent, which can effectively promote growth and development of a calf, raises daily gain, weans calf early, reduces bad effects of weaning stress on a calf, and reduces diseases.

The invention relates to a method for producing transgenic buffalo embryos by applying intracytoplasmic sperm injection (ICSI) mediation. The method comprises the following steps of: 1, incubating buffalo sperms and transgenic deoxyribonucleic acid (DNA) together; 2, performing intracytoplasmic microinjection on the buffalo sperms; and 3, cultivating and identifying the transgenic buffalo embryos. By the method of the invention, the transgenic buffalo embryos are obtained successfully. In 102 injection spawns of the transgenic buffalo embryos, 61.8 percent of the injection spawns are subjected to cleavage, 17 blastospheres are obtained in embryos expressed by blastomere serving as enhanced green fluorescent protein (EGFP), 11 blastospheres express the EGFP, and the transgenic positive rate of the blastospheres is up to 64.7 percent; and after one of the transgenic embryos is cultured in vitro for a long time, the expression of the EGFP can be still seen during 2 to 7 days, so the method makes the important progress for producing transgenic buffalos further.

The invention relates to a fermented milk product with a blood pressure reducing function and a preparation method thereof and belongs to the technical field of milk product processing. Water buffalo milk is adopted as a raw material and combined with saccharose, a water buffalo milk antihypertensive peptide base material, taurine and a lactic acid bacterium strain, and the fermented milk product with the blood pressure reducing function is prepared. The preparation method of the fermented milk product is simple, and the prepared yogurt is strong in milk fragrance, creamy in texture, fine and smooth in taste and rich in nutrition. All nutritional value of the water buffalo milk can be kept, meanwhile, the blood pressure reducing effect is improved, and the healthcare effect of the fermented milk product is improved. The prepared fermented milk product is free of any additive, long in shelf life, safe, environmentally friendly and healthy.

The invention relates to the technical field of molecular markers, and discloses a method for identifying a molecular marker of a candidate gene related to milk production traits of buffaloes. The method comprises the following specific steps: (1) obtaining SNP genotypes of 45 buffalo individuals by a dd-RAD method; (2) performing grouping based on population genetic diversity and population structures, further performing selection signal analysis by using Fst and XP-CLR strategies, and identifying the candidate gene related to the milk production traits; (3) using a comparative transcriptomestrategy to assist in verifying the relevance between the candidate gene and the milk production traits of the buffaloes; and (4) identifying the related molecular marker influencing the milk production traits of the buffaloes by using a marker-trait correlation analysis strategy. The candidate gene SCLO3A1 related to the milk production traits of the buffaloes and the molecular marker g.54621870G>T influencing the milk protein rate are identified by the method for the first time. The molecular marker can be used for breeding a buffalo population with high milk protein content.

The invention belongs to the technical field of preparation of livestock molecular markers, and particularly relates to an application of a buffalo milk production trait related Leptin gene G3683T locus as a molecular marker to buffalo lactation performance research. The method is characterized in that a nucleotide sequence of a Leptin gene fragment is represented as SEQ ID NO:1, wherein the 3683th locus has G/T base mutation, the locus is remarkably related with milk production of buffalo species, and P is smaller than 0.05. The invention discloses sequences of upstream and downstream primers required for detecting the G/T base mutation at the 3683th locus represented as a sequence table SEQ ID NO:1. The novel molecular marker method is provided for buffalo milk production trait markers.

The invention discloses a method for identifying single-parent buffalo paternity relationship as well as primers and kits thereof, belonging to the technical filed of genetic engineering. The method comprising the steps: screening out eight microsatellite primers with rich polymorphism; PCR amplifying by taking detected buffalo genome DNA as a template; detecting PCR amplified fragment through polyacrylamide gel electrophoresis; judging individual genotype according to the detected result; counting single-parent paternity index PI value and parent-child relative probability value W value according to the genotype; and judging whether the paternity relationship is existed or not according to the W value. In order to further improve identification stability, accuracy and sensitivity, the method designs primers so as to identify mitochondrial inheritance according to the D-Loop region of common cattle mtDNA. The method combines microsatellite method with mitochondrial inheritance marker method, and identifies from different angles and reaches consistent result, thereby improving identification stability, accuracy and sensitivity and having the advantages of wider identifying range and lower sample requirement.

The invention is concerned with a kind of Chinese traditional medicine compounding and its preparation method to cure ischemia type apoplexy. This compounding is a compound Chinese materia medica preparation made of cornu satgae, gastrodia tuber, buffalo horn concentrated powder, rhubarb, love ipea seed, notoginseng, red sage root, earthworm, pangolin, chuanxiong rhizome, Radish seed semen raphani, and moschus musk in weight proportion. Grind gastrodia tuber, peach kernel, red sage root, earthworm, chuanxiong rhizome and Radish seed semen raphani, extract with water and alcohol, concentreate, dry and grind into fine powder, mix with buffalo horn concentrated powder to get common preparation. It is fit to cure ischemia type apoplexy caused by wind phlegm, blood stasis, impotency skeleton with perfect clinical treatment purpose and high safety.

The invention provides a SREBP1 gene related to lactation of a buffalo, and application of the same as a molecular marker, belonging to the field of molecular markers for livestock. The nucleotide sequence of the gene is as shown in SEQ ID No. 1, wherein C8358T and G15306T single-base mutation occurs at site 8358 bp and site 15306 bp, respectively; the SNP site at the 8358 bp is substantially related to the milk output of the buffalo; and the SNP site at the 15306 bp is substantially related to the milk output, milk protein rate and lactose rate of the buffalo. The invention discloses the sequences of upstream and downstream primers needed by all the base mutation sites in SEQ ID No. 1 in a detection sequence table. Application of the SREBP1 gene is beneficial for improving the milk production performance of the buffalo and provides technical support and theoretical foundation for breeding of milk buffalos with high milk output, high milk protein rate and high lactose rate of produced milk.

The invention discloses Bacillus subtilis X3 and application thereof for reducing generation of hydrogen sulfide in buffalo dung. The Bacillus subtilis X3 is preserved in China Center for Type Culture Collection on June 16, 2013, the serial number is CCTCC NO: M2013261, and the preservation address is Wuhan University, Wuhan, China. Soil and the like in a buffalo farm are used as strain separation sources, the Bacillus subtilis X3 is cultured at 37 DEG C for 24h and is directly inoculated into a sealed beaker filled with buffalo dung in a proportion of 20% (W/W), and the Bacillus subtilis X3 is cultured for 2-8 days at room temperature to reduce hydrogen sulfide. Compared with a control group added with an equal proportion of sterile water, the Bacillus subtilis X3 can reduce the generation of hydrogen sulfide in the buffalo dung by 19.59-52.74% and has the advantages of high efficiency, low nutrition requirements, simple operation and high safety.

The invention relates to the field of animal molecular markers, in particular to an obtaining method and application of SNP related to milk buffalo milk production traits. The application of five SNPloci of a buffalo in buffalo milk production traits or molecular breeding analysis is provided. Nucleotide sequences of the five SNP molecular markers are shown in SEQ ID NO.1-5. The invention furtherdiscloses a buffalo milk production trait or molecular breeding analysis method. The five SNP loci can provide support for upstream and downstream processes of breeding, and have the advantages of high determination accuracy and the like; the milk production performance of a milk buffalo can be improved, and technical support is provided and a theoretical basis is laid for breeding of the milk buffalo with excellent milk production performance.

The invention discloses a moist and regenerative burn liquid, which is prepared by decocting 80-120g of buffalo horn, 80-120g of honeysuckle flower, 80-120g of radix ophiopogonis, 40-80g of gardenla jasminoides Ellis, 80-120g of trichosanthes root, 80-120g of dahurian angelica root, 80-120g of raw rehmannia root, 40-80g of akebia stem and 80-120g of dandelion with water. The burn liquid of the invention has efficacies of clearing heat, detoxifying, diminishing inflammation, easing pain, removing necrosis, promoting granulation, moistening intestines, relaxing bowel and purging fire, and has characteristics of no scar after healing, no disability, no disfigurement, no granulation hyperplasia, no pigmentation, no functional disorder, no anodyne injection or anodyne administration during treatment. Besides, the burn liquid can eliminate pains in 20 minutes in order to rapidly eliminate pains, fantod, anxiety and mitigate mental pressure of a patient. The burn liquid of the invention has a short treatment period and low treatment costs; the wound can be scabbed in 7-12 days and shed automatically; and the patient can keep normal defecation, dietary and mental state during the treatment.

The present invention provides ointment, suppository, and herbal tea modalities for the treatment of hemorrhoids. The ointment and suppository modalities include the extracts of Nigella sativa, leek seed powder, Adeps camel, cow butter oil (Friesian), cow butter oil (Jersey), water buffalo butter oil, sheep butter oil, goat butter oil, coconut oil, and farm cow butter oil. The herbal tea modalities include Mentha piperita, Peppermint (American Mentha), Mentha aquatic, and Mentha longifolia (Moroccan mint).

The invention discloses a feed capable of reducing methanogenesis of gastrointestinal tracts of buffalos. The feed is composed of following components in percentage by mass (on the basis of air dry matters): 25.00%-27.00% of corns, 7.50%-11.25% of soya bean meal, 3.75%-7.50% of beer residues, 25.00%-27.00% of silo corns, 22.50%-26.25% of pennisetum purpureum schum, 2.00%-7.50% of fermented cassava residues, 0.13%-0.33% of cysteamine hydrochloride and 1.00%-3.00% of sodium bicarbonate. According to the feed, the methanogenesis of the gastrointestinal tracts of milk buffalos is reduced by scientifically matching a rough feed and combining and adding the cysteamine hydrochloride. Different from other methods, the cysteamine hydrochloride is added on the basis of an authorized patent, namely a formula of a feed capable of reducing methanogenesis of gastrointestinal tracts of buffalos (the application number is 201110189541.X; an authorization patent number is ZL201110189541.X), so as to reduce the emission amount of methanol of the gastrointestinal tracts of cud chewers.

The invention belongs to the technical field of livestock molecular marker screening, and particularly relates to a haplotype combination relevant to the Mediterranean buffalo good milk secretion property and application. The molecular marker is obtained through the clone from the Mediterranean buffalo; 12 SNP markers relevant to the milk secretion property are obtained through screening; the haplotype formed by one marker or the combined markers is used for performing relevance analysis on the Mediterranean buffalo milk secretion property. The sequences of the 12 SNP markers of the ACSL1 geneare shown by SEQ ID NO: 1 to SEQ ID NO: 12. The invention also relates to development of detection primer of ACSL1_SNP1 and ACSL1_SNP12 site genotypes and application of good haplotype combined sequences. The haplotype combined sequences are obviously relevant to the Mediterranean buffalo peak milk quantity, 270d milk yield and 270d milk protein quantity properties. The invention provides a novelgenetic marker resource for the marker auxiliary selection of the Mediterranean buffalo.

The invention discloses a babesia orientilas thrombin gene 1 which has the nucleotide sequence shown in SEQ ID NO:1. The invention further discloses recombinant protein obtained by coding the babesia orientilas thrombin gene 1, wherein the recombinant protein has the amino acid sequence shown in SEQ ID NO:2. The recombinant protein can effectively detect the existence of antigens of a water buffalo infected with the babesia orientilas, has good reactogenicity, belongs to a candidate factor for developing a serological diagnosis method for detecting the babesia orientilas disease, and is beneficial for controlling the prevalence and spread of the babesiosis disease of the water buffalo.

The invention relates to preparation of rumen bypass coated bacteriophage and an application of the rumen bypass coated bacteriophage in prevention and treatment of buffalo diarrhea. The rumen bypass coated bacteriophage is prepared by compounding six bacteriophages, comprising a staphylococcus aureus bacteriophage 52, a staphylococcus aureus subspecies bacteriophage B1, an escherichia coli bacteriophage T1, an escherichia coli bacteriophage T3, an escherichia coli bacteriophage S13 and a bacillus subtilis bacteriophage phi-e. The rumen bypass coated bacteriophage provided by the invention is added into a buffalo concentrate supplement according to the proportion that 30-70kg of the rumen bypass coated bacteriophage is added into each ton of concentrate. The rumen bypass coated bacteriophage is administrated to buffalo in a rumen bypass coating mode, the effect of preventing and treating buffalo diarrhea is ideal, and reference is provided for research of screening specific bacteriophages in the later period.

The invention provides a buffalo calf lick brick and a preparation method thereof, and belongs to the field of feeds. The buffalo calf lick brick is prepared from the following raw materials in partsby weight: 200 to 400 parts of hydroxymethyl cellulose, 250 to 450 parts of bentonite, 2800 to 3200 parts of feed salt, 400 to 500 parts of urea, 100 to 130 parts of anhydrous calcium hydrophosphate,120 to 150 parts of mineral additives, 400 to 600 parts of composite vitamins, 0.2 to 1 part of acetohydroxamic acid, 1 to 3 parts of lactoferrin, 1 to 5 parts of rumen bypass protein, 1 to 5 parts ofalgal polysaccharide, 1 to 5 parts of yeast cell wall polysaccharide and 20 to 50 parts of plant extracts. The buffalo calf lick block is rich in nutrition and capable of maintaining electrolyte balance of calf bodies, preventing and treating mineral nutrient deficiency, promoting growth, improving feed conversion, improving resistance and preventing and treating ticks.

The present invention relates to a Chinese medicine prescription for curing chronic myeloid leukemia and a preparation method thereof, pertaining to the art of Chinese medicine. The internal prescription includes 20 to 40g of radix notoginseng, 10 to 40g of field thistle, 15 to 40g of pilose deer horn, 20 to 45 of lotus powder, 20 to 50g of prepared rehmannia rhizome, 20 to 55g of donkey-hid gelatin, 20 to 60g of dangshen, 20 to 40g of milk veteh, 20 to 40g of carbonized sanguisorba root, 15 to 40g of saffron, 10 to 40g of medlar, 15 to 45g of carbonized buffalo horn, 15 to 50g of Chinese caterpillar fungus, 10 to 40g of carbonized leatherleaf millettia, 10 to 45g of carbonized black plum and 10 to 50g of carbonized hair. The external prescription includes 5 to 15g of carbonized radix notoginseng, 5 to 20g of carbonized setose thistle, 5 to 25 carbonized field thistle, 5 to 15g of carbonized human hair, 5 to 20g of carbonized petiole of windmill palm, 5 to 15g of carbonized rehmannia root and 5 to 25g of common bletilla. The oral powder made by smashing the internal materials is added with honey and made into honey pills or added with water and made into waterdewed pills. The external materials are smashed to obtain the external powder. Patients with chronic myeloid leukemia select honey pill or waterdewed pill and have one pill before three meals a day; the external powder is coated on the affected part by a tampon. Patients with chronic myeloid leukemia use the present invention both for external use and oral administration.

The invention provides a molecular marker related to white hair phenotypes of buffaloes and an application. The molecular marker is developed based on buffalo whole genome association analysis, and comprises 2 SNP molecular markers, namely T/C mutation of the 101st base of the sequence shown in SEQ ID NO.1 and G/A mutation of the 101st base of the sequence shown in SEQ ID NO.2. The molecular marker provided by the invention can be used for genetically identifying white hair phenotypes and tile grey hair phenotypes of buffaloes, can be used for auxiliary breeding of white hair buffaloes havingbuffalo or buffaloes having tile grey hair, and has a good application prospect.

The invention discloses cloning of a buffalo milk production character MC4R gene and an application thereof serving as a molecular marker. A sequence obtained by cloning is as shown in SEQ ID NO:1, three base mutations affecting the buffalo milk production character are detected, which are respectively C226-T226 at 226bp, C1104-T1104 at 1104bp and A1525-G1525 at 1525bp. The invention discloses a buffalo gene MC4R sequence, 3 base mutation sites affecting the buffalo milk production character, a method for obtaining the gene and an application thereof, and provides a new molecular marker for auxiliary selection of buffalo milk production character markers.

The present invention provides a buffalo embryo freezing method in combination with laser membrane rupture. The method comprises: culturing an in vitro fertilized buffalo embryo to be an expanded blastocyst; using a laser membrane rupture instrument to drill a blastocyst cavity; discharging blastocyst fluid; and then using freezing liquid to perform vitrification freezing. According to the present invention, discharging the blastocyst fluid before freezing can make the cryoprotectant enter an embryo more quickly, reduce the possibility of ice crystal forming in a cell, and thereby avoiding embryo damage to a large extent, and improving a freezing survival rate of the embryo. On the other hand, due to partial breaking of a zona pellucida before freezing, so that after thawing cultivation, the zona pellucida is easier to be hatched in a revived blastocyst cavity, and thereby facilitating embryo implantation of the embryo after transplanting, and improving a transplant pregnancy rate.