26 results about "Water buffalo" patented technology

The invention is a divisional application with the patent application number of CN202510138171.9. The invention discloses an application of an SNP (Single Nucleotide Polymorphism) site related to buffalo lactation traits and an application of an amplification primer group of the SNP site, the SNP site is used for detecting dominant genotypes related to the buffalo lactation traits, the SNP site is marked as the 51st basic group in Chr6116041915, the basic group is G or A, and the amplification primer group is marked as the 51st basic group in Chr6116041915. The nucleotide sequence of the Chr6116041915 is specifically as shown in SEQ ID NO. 3. Compared with the prior art, one SNP molecular marker related to the buffalo lactation character is screened and extracted and is used for breeding buffalo individuals with high daily average milk yield, the breeding time is effectively shortened, and the breeding cost is reduced.

The invention discloses a buffalo rumen source lignin and cellulose degrading bacterium microecological preparation as well as a preparation method and application thereof, and relates to the technical field of microorganisms. The active ingredient of the microecological preparation comprises bacillus sorola desert, and the preservation number of the bacillus sorola desert in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2025512. According to the invention, a strain of bacillus sorola, which has lignin degradation ability and can degrade cellulose, is screened from a rumen solid-liquid mixture sample obtained from a buffalo rumen fistula, and is developed into a microecological preparation. Experimental verification shows that the degradation rate of the strain to sodium lignin sulfonate in 24 hours reaches 9.54%, the degradation rate of the strain to straw in 3 days can reach 8.6% or above, and in an artificial simulation rumen test, lignin in the straw can be remarkably degraded, and meanwhile, the fiber utilization value of the straw is effectively improved.

This invention relates to the field of buffalo breed identification, specifically to a method for buffalo breed identification and a method and apparatus for constructing a prediction model. The prediction model construction method includes: acquiring second-generation NGS sequencing data of a buffalo population; comparing the sequencing data with a reference genome and performing variant detection to obtain variant site data for each buffalo; merging and filtering the variant site data of all buffaloes to obtain a variant site set; and training the buffalo breed prediction model using the variant site set data to obtain the prediction model for the buffalo breed. The prediction model of this invention achieves high accuracy in buffalo breed identification.

The invention belongs to the technical field of animal molecular biology and animal genetic breeding, and particularly relates to a buffalo whole genome 20k liquid-phase SNP chip and a design method and application thereof, and the chip comprises 24893 SNP molecular markers; according to the method, four selection signal analysis (FST, Piratio, XP-CLR and XP-EHH) are jointly applied to screen 11 buffalo group comparison combinations, it is ensured that the sites have high information content and group universality, through multiple rounds of quality control and function annotation, finally 24893 SNP sites are carefully selected, the whole genome is covered in a balanced mode, and the redundancy is remarkably reduced; based on 115 sample data of 11 main Chinese buffalo varieties (including marsh type and river type), the chip is ensured to be suitable for multi-variety buffalo groups, and the application advantages of variety identification, genetic analysis, genetic diversity monitoring and the like are highlighted.

The invention belongs to the technical field of food processing, and discloses additive-free buffalo formula milk and a preparation method thereof. The raw materials of the additive-free formula milk are pretreated buffalo milk and milk; wherein the mass fraction of the pretreated buffalo milk is 30-45%, and the preparation method comprises the following steps: cooling the buffalo milk to 0-4 DEG C, then carrying out centrifugal treatment to obtain milk liquid and cream enriched with large-particle-size fat globules, strongly crushing the fat globules in the cream, then carrying out stabilizing treatment, and then uniformly mixing with the milk liquid. Through a pure physical process of centrifugation and segmented homogenization, the problem that the formula milk with the buffalo milk content of 30-45% is greasy in mouth feel due to too high fat content is solved on the premise that natural characteristic flavor substances in the buffalo milk are reserved to the maximum extent, and the smooth mouth feel and good preservation stability of the product are ensured under the condition that no additive is added.

The invention discloses a lactation buffalo production performance regulation and control method based on rumen energy nitrogen balance regulation and control, and relates to the technical field of lactation buffalo rearing, the method comprises the steps of obtaining and fusing rumen internal environment parameters, individual ingestion behaviors and production performance data of lactation buffalo, and obtaining a multi-source data fusion analysis result through multi-source data fusion analysis; generating a rumen-physiological dynamic metabolic potential energy map; based on a rumen-physiological dynamic metabolic potential energy map, performing digital twinborn deconstruction analysis on daily ration composition to obtain energy nitrogen libraries with different degradation characteristics; the ideal supply gap spectrum of each energy nitrogen library is dynamically calculated in combination with physiological time sequence parameters obtained in real time; and according to the ideal supply gap spectrum of each energy nitrogen reservoir, precise nutrition intervention is cooperatively executed through time sequence matching feeding, form adaptation regulation and control and targeted supplement regulation and control. The problems of lactation performance fluctuation, low feed utilization efficiency and high metabolic stress risk caused by energy nitrogen imbalance are solved.

The invention belongs to the technical field of molecular biology, and relates to miRNA for regulating and controlling the content of fat and / or unsaturated fatty acid in buffalo muscle and application of the miRNA. The miRNA is miR-30f (bta-miR-30f), and the fat deposition in the buffalo muscle and the composition of unsaturated fatty acid are influenced by regulating and controlling the expression of an RAD23B gene. The invention finds that transfection of a bta-miR-30f simulant in buffalo intramuscular fat cells can significantly improve intramuscular fat content and unsaturated fatty acid proportion, while transfection of an inhibitor shows a reverse effect. Further research finds that knock-down of the RAD23B gene can also increase the content of triglyceride and unsaturated fatty acid in buffalo muscle tissue. Important gene resources and molecular breeding technical means are provided for genetic improvement of buffalo meat quality characters (including flavor characteristics and nutritional value).

The application discloses a Haizi buffalo whole-genome SNP molecular marker combination and application. The SNP molecular marker combination is shown in Table 1 and is composed of 47943 sequencing-derived high-heritage-information SNPs, 868 trait-related SNPs and 76 defect-related SNPs, and a total of 48887 sites. The Haizi buffalo whole-genome SNP molecular marker combination provided by the application can be used for design of a Haizi buffalo whole-genome chip and protection and breeding of Haizi buffalo genetic resources, can realize targeted gene detection work on a Haizi buffalo population, can meet various requirements in genetic resource protection and breeding, can effectively improve molecular breeding efficiency, and has important significance for protection, evaluation and utilization of Haizi buffalo genetic resources.

The present application relates to the field of microbial technology, in particular to bacillus subtilis BS-1 and its application in water buffalo silage feed, the strain BS-1 of the present application has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, after mixing and fermenting according to a specific proportion with orange peel and cassava residue, the bacteriostatic spectrum of the obtained silage feed is significantly enhanced, which can simultaneously inhibit escherichia coli, campylobacter jejuni, salmonella, streptococcus uberis, staphylococcus aureus and streptococcus agalactiae that cause diarrhea and mastitis. In the feed, after mixing 60%-75% of orange peel and 25%-40% of cassava residue, the crude protein content of the fermentation product is significantly improved, and the fiber content is reduced. Feeding test shows that the feed can significantly improve the milk yield and milk quality of lactating water buffalo, and effectively alleviate diarrhea. The present application provides a safe, efficient and nutritious silage feed with disease prevention function.

This invention relates to the design of specific primers for buffalo (Bubalus bubalis) cytochrome b (Cytb) gene for the detection of buffalo DNA using conventional PCR method. The primer sequence of buffalo species specific Cytb gene consists of forward sequence 5'- TTAGTACTATTCGCACCCGACCTC-3' and reverse 5'- TCGTTGTTTGGATGTATGTAGCAG-3'. This primer sequence has an amplicon size of 216 bp. The primers were used in a conventional PCR process operated under the following conditions, initiation stage of denaturation at 95ºC for 60 seconds, denaturation stage at 95ºC for 15 seconds, annealing stage at 63ºC for 15 seconds, elongation at 72ºC for 10 seconds cycle, final elongation at 72ºC for 60 seconds and at 35 cycles. This invention aims to amplify buffalo species specifically using conventional PCR methods so that it can detect the presence of buffalo DNA in processed products, especially for processed buffalo skin crackers.

The invention provides a yellow-causing bacterium capable of being used for in-vitro culture of calculus bovis and application of the yellow-causing bacterium, and relates to the technical field of microorganisms, the yellow-causing bacterium is obtained by separating and culturing natural yak bile, and not only has better yellow-producing capacity, but also has higher tolerance to bile. The yellow-causing bacteria are obtained by screening through an optimized screening strategy, gram-positive yellow-causing bacteria can be obtained through the screening strategy, and the screening range of potential yellow-causing bacteria is expanded. The xanthogenic bacterium can directly take bile of natural yaks, cattle, buffalo and other bovine animals as a raw material to perform in-vitro calculus bovis culture, so that the product is closer to the formation process of natural calculus bovis, has the potential of being developed into a natural calculus bovis substitute, and has a better application prospect in the aspect of in-vitro calculus bovis culture.

The application discloses a kind of based on Internet of Things's water buffalo precise feeding system and method. Including biological feature acquisition terminal, edge computing device, cloud intelligent platform and execution control module, it is applicable to the intelligent management scene of large-scale water buffalo breeding. The method is by collecting the nose print image of water buffalo, voiceprint signal and temperature data, fusion multi-modal biological feature realizes individual identity recognition;On the basis of identification, call the dynamic nutrition model constructed based on GRU neural network, combine weight, exercise amount, environmental parameter and other characteristics to generate individualized feeding formula;Adopt mixed integer programming algorithm to optimize feed proportioning, give consideration to nutrition, cost and palatability Multiple constraints;It also includes abnormal detection and nutrition compensation mechanism, for identifying healthy abnormal individual and adjusting feeding scheme. The system is based on Internet of Things technology to realize edge inference and cloud cooperation, improve the identification accuracy, response speed and feeding intelligent level.

The present application relates to the technical field of biology, in particular to buffalo mammary gland tissue digestion solution and application thereof, and provides a preparation method of buffalo mammary gland tissue digestion solution and buffalo mammary gland tissue single-cell suspension, and the preparation method can obtain a primary cell suspension of buffalo mammary gland tissue with high viability, low clumping rate, high nucleated rate and less impurities, and can greatly improve the success rate of single-cell sequencing experiment of buffalo mammary gland tissue.

The invention provides a sequence combination for identifying cattle-derived components, buffalo-derived components and yak-derived components based on RPA-CRISPR / Cas12a and application of the sequence combination, and belongs to the technical field of biological detection.The sequence combination for rapidly detecting the cattle-derived components, the yak-derived components and the buffalo-derived components based on RPA-CRISPR / Cas12a comprises an RPA primer pair for detecting the cattle-derived components, the yak-derived components and the buffalo-derived components and a specific crRNA sequence, the invention also discloses a kit prepared by using the sequence combination and a detection method for detecting cattle, yak and buffalo-derived components in food by using the kit. According to the detection method disclosed by the invention, an RPA amplification method is combined with a CRISPR / Cas12a detection method, and real-time detection is carried out by detecting a fluorescence signal; the detection method disclosed by the invention is simple and rapid, has good specificity and sensitivity, can be used for detection only by simple constant-temperature heating, and has the advantage of rapidly detecting cattle, yak and buffalo derived components on site.

This invention concerns immunogenic B cell epitopes of the RPO30 protein of the Lumpy Skin Disease virus isolate from East Java as vaccine candidates in the field of animal health. This invention relates to the identification, characterization, and utilization of immunogenic B cell epitopes derived from the RPO30 protein of the Lumpy Skin Disease virus isolate from East Java. The identified B cell epitopes were selected from the group consisting of SL, D, TSSYESTSSQ, and QGMGGYT, which have the ability to induce specific humoral immune responses against the Lumpy Skin Disease virus. These B cell epitopes can be chemically synthesized or recombinantly expressed and formulated with a veterinary acceptable carrier or pharmaceutical adjuvant to produce an epitope-based vaccine candidate. This invention provides an alternative vaccine candidate that is safer than live attenuated vaccines, has the potential to reduce post-vaccination side effects, and is more specific to local Indonesian isolates.This invention is expected to be used for the prevention of Lumpy Skin Disease in cattle and buffalo.

The present invention relates to the technical field of animal genetics and breeding, and in particular, to an SNP molecular marker for identifying or assisting in identifying a black coat color phenotype in water buffaloes and use thereof. Provided is an SNP molecular marker for identifying or assisting in identifying a black coat color phenotype in water buffaloes. The SNP molecular marker is located at a base position 14298688 on chromosome 18 of a water buffalo genome. When the base at the position is T, the coat color of the water buffalo is pure black; the version number of the water buffalo genome is UOA_WB_1. The provided molecular marker is an SNP molecular marker related to the black coat color phenotype of water buffaloes and can be used alone or in combination to genetically identify the body coat color of water buffaloes. According to the different needs for target traits in water buffalo breeding, water buffaloes homozygous for black coat color genes can be selected for marker-assisted breeding, thereby providing a convenient and accurate method for the breeding of water buffaloes with different coat colors.

The invention relates to a bionic rhinoceros horn biological agent and a preparation method, application and identification method thereof, the bionic rhinoceros horn biological agent comprises the following components in percentage by weight: 40-50% of recombinant keratin, the recombinant keratin has an amino acid sequence as shown in SEQ ID NO: 1, or the recombinant keratin has a functional variant which has sequence identity of more than or equal to 80-95% with SEQ ID NO: 1 and has an inhibition rate of more than or equal to 70% for LPS-induced TNF-alpha; the molecular weight of the cornu bubali enzymolysis active peptide is less than 5kDa, and the arginine content of the cornu bubali enzymolysis active peptide is greater than or equal to 12.3 mg / g; 15%-25% of silk fibroin; the content of Ca < 2 + > in the trace elements is 3.0%-3.5%. The rhinoceros horn can be used for replacing natural rhinoceros horns and is suitable for the field of emergency treatment of febrile convulsion, cerebral hemorrhage coma and the like.

The invention discloses a buffalo rumen-derived lignin degrading bacterium composite microecological preparation and application thereof, and relates to the technical field of biology. Active components of the buffalo rumen-derived lignin degrading bacterium composite microecological preparation comprise a buffalo rumen-derived lignin degrading bacterium combination; the buffalo rumen source lignin degrading bacterium composition is prepared from bacillus sorola desert, bacillus cereus and ochrobactrum pseudointermedium. The buffalo rumen source lignin degrading bacterium composite microecological preparation can obviously improve the digestion and absorption capacity of beef cattle, and especially has an obvious degradation effect on wood fiber substances which are difficult to decompose, so that the feed utilization rate is increased, and the feeding cost is reduced.

The present application belongs to the technical field of traditional Chinese medicine, and relates to a traditional Chinese medicine composition for treating thrombotic arteriopathy. The traditional Chinese medicine composition contains radix rehmanniae 20-40 parts, red peony root 10-20 parts, radix paeoniae 10-20 parts, radix lithospermi 10-20 parts, water buffalo horn 10-20 parts, forsythia 20-40 parts, radix codonopsis 20-40 parts, white atractylodes 10-20 parts, poria 20-40 parts, sedum 20-40 parts, ladybell 20-40 parts, sophora 10-20 parts, caper 10-20 parts, radix clematidis 10-20 parts, big belly skin 20-40 parts, and licorice 5-14 parts. The traditional Chinese medicine composition has a good therapeutic effect on thrombotic arteriopathy, has a high clinical treatment efficiency, can obviously improve the clinical symptoms of patients, reduce the pain of patients, and pharmacological experiments show that the traditional Chinese medicine composition can significantly reduce the inflammatory symptoms of rats.

The application relates to the field of biotechnology, and particularly relates to a method for obtaining a biological marker of a buffalo estrus period and application of the biological marker to identifying the buffalo estrus period, S1, collecting milk samples of a female buffalo, and observing follicle development of the female buffalo by using B ultrasonic waves; S2, after the milk samples are extracted, analysis is conducted by using a quadrupole-electric field orbitrap high-resolution mass spectrometry system and an ultrafast high-performance liquid chromatography system; S3, after original data are converted into an mzXML format, extraction data are obtained by using XCMS software processing, the extraction data are processed and analyzed, and significant difference metabolites of cow milk in an estrus period are screened out; S4, expression amounts of the significant difference metabolites of the cow milk in the estrus period and a non-estrus period are detected, the expression amount changes are compared with B ultrasonic wave results, and a biological marker of the buffalo estrus period is obtained. Compared with the prior art, pyridoxamine and homovanillic acid are obtained as the biological marker of the estrus period of the female buffalo, and are used for identifying whether the female buffalo is in the estrus period.

The present application relates to the technical field of animal feed. The present application provides a kind of growing period buffalo feed and its preparation method, the feed includes the following weight parts of component: total mixed silage feed 60-90 parts, fermented wu citrus peel 8-12 parts, fermented passion fruit peel 12-18 parts.Wu citrus peel residue and passion fruit peel residue of the present application are fermented after containing bacillus circulans, fructophilic lactic acid bacteria, sharp's bacteria complex lactic acid bacteria and complex enzyme containing laccase, cellulase, pectinase, glucose oxidase, and mixed with total mixed silage fermented by complex lactic acid bacteria, the obtained feed can promote the growth of growing period buffalo, and has obvious inhibitory effect on diarrhea of growing period buffalo.

The present invention discloses a kit and a novel method of extraction of a progesterone metabolite from feces / dung sample. The present invention also discloses a paper-based microfluidic device for detection of the progesterone metabolite for early determination of pregnancy in cattle and buffaloes. The invention further discloses antibodies developed against said metabolite, method of extraction, methods for fabricating the paper-based microfluidic devices and methods for inexpensive, rapid and non-invasive determination of pregnancy in early stages in cattle and buffaloes.

The present application relates to the technical field of breeding, in particular to a system for controlling nitrogen to energy ratio by monitoring fat production of lactating buffaloes, comprising a detection shed, a weighing assembly fixedly installed in the middle of the inside of the detection shed, a positioning assembly fixedly installed at the top of two fences, and a measuring assembly fixedly installed at the bottom of a vertical moving block. Compared with the prior art, the present application is provided with the weighing assembly and the measuring assembly. In the process of walking, the buffaloes pass through the weighing assembly in the detection shed to record the body weight, and at the same time, the measuring assembly is closely attached between the neck and the back of the buffaloes, the electrode sheet is connected with the skin of the buffaloes, and with the advance of the buffaloes, the longitudinal moving block follows the movement on the slide rail to ensure the measurement time. At this time, the electrical impedance instrument measures and analyzes the body fat, and finally the detailed body fat data and the body weight data are presented on the display screen to provide intuitive support for comprehensively understanding and comparing the health status of the buffaloes.

This invention concerns the RPO30 protein of an Indonesian isolate of Lumpy Skin Disease virus and its use in the development of an epitope-based vaccine in the field of animal health. This invention relates to the provision of the RPO30 protein or its immunogenic B cell epitope fragment derived from an Indonesian isolate of Lumpy Skin Disease virus as an active vaccine ingredient. The RPO30 protein or the immunogenic B cell epitope fragment is identified through genetic sequence analysis and immunogenicity prediction, then chemically synthesized or recombinantly expressed. The immunogenic B cell epitope fragment is then formulated with a veterinary acceptable carrier or pharmaceutical adjuvant to produce an epitope-based vaccine. This epitope-based vaccine is designed to induce a specific humoral immune response against Lumpy Skin Disease virus without the use of live virus.This invention provides an alternative for developing a safer vaccine, specific to local Indonesian isolates, and potentially reducing post-vaccination side effects. It is hoped that this invention can be used to prevent Lumpy Skin Disease in cattle and buffalo.

The invention discloses a buffalo milk collecting device. The buffalo milk collecting device comprises a shell, a lining and a radial adjusting mechanism, the radial adjusting mechanism is provided with at least two sets of adjusting units which are independently arranged in the axial direction of the shell, and each set of unit controls the pressurizing parts in the circumferential array to synchronously move in the radial direction through an adjusting piece. Each group of adjusting units can independently and accurately control the radial shrinkage amount of different axial sections (such as the root and the middle) on the lining. And the inner cavity of the lining can actively deform to form a stepped adaptive shape matched with the natural taper of the buffalo nipple, so that the full-length uniform and fit pressure distribution is realized. The vacuum sealing reliability in the milking process is improved, efficient and mild milk emptying is ensured, the risk of nipple tissue damage caused by too high local pressure or sealing air leakage is remarkably reduced, and the vacuum sealing device is particularly suitable for efficient and healthy collection of buffalo milk.