40 results about "Bovine milk" patented technology

The invention belongs to the technical field of dairy product detection, and relates to a characteristic peptide fragment for identifying alpha-casein of cattle and sheep milk, a qualitative identification method and application. The characteristic peptide fragment comprises a characteristic peptide fragment of cow milk alpha-casein, and the characteristic peptide fragment of the cow milk alpha-casein is CAGCPHCPDHWMGYGDHCYYFSVEK or EYSTAHLSRTLTLVEQIK; the characteristic peptide fragment of the cow milk alpha-casein is CAGCPHCPDHWMGYGDHCYYFSVEK; the characteristic peptide fragment of the goat milk alpha-casein is SLCQETCCSPSCCQTTCCRTTCYR, and the characteristic peptide fragment of the goat milk alpha-casein is According to the method disclosed by the invention, the characteristic peptide fragments of cow milk alpha-casein and goat milk alpha-casein are screened by combining enzymolysis, high performance liquid chromatography and high-resolution mass spectrometry, and then the alpha-casein characteristic peptide fragments are used for qualitatively identifying cow milk and goat milk, so that the detection precision is high, and the accuracy of an identification result is high; and adulteration identification of cow milk and goat milk can be realized.

This invention relates to the field of emulsion technology, specifically disclosing a method for preparing a casein micelle-phenol-whey protein ternary complex, comprising: S1, screening bovine milk with κ-casein genotype AA, and using it as raw material to prepare a casein micelle solution; S2, adding a phenolic compound to the casein micelle solution to form a casein micelle-phenol binary complex mixed solution; S3, dialyzing the mixed solution obtained in step S2; S4, adding whey protein; by combining a phenolic compound (such as EGCG) with casein micelles and whey protein to form a ternary complex, the degree to which proteins form dense clots in the stomach can be reduced, making the clot structure looser, thereby accelerating the digestion rate and reducing stomach discomfort.

The invention belongs to the technical field of biology, and discloses a molecular marker for detecting the character of fatty acid in cow milk and an identification method. The molecular marker SNP provided by the invention is g.16772853G / T, and is related to the contents of fatty acids C14: 0, C14: 1, C16: 1, C17: 0 and C18: 0 of cow milk. By utilizing the SNP provided by the invention, early screening can be realized in the dairy cow breeding process, the breeding cost can be reduced, and the fatty acid content of milk can be effectively increased or reduced according to production requirements. The method is high in detection accuracy and low in cost, and has an important application prospect in dairy cow breeding.

The powdered exosomes isolated from bovine milk contain intact exosomes. The nutritional composition contains protein, carbohydrates, and / or fat, and exosomes isolated from bovine milk. A method for preparing the powdered exosomes includes centrifuging bovine milk to form a first precipitate of an upper lipid fraction, a middle whey fraction, and cells and cell debris; separating the whey fraction and centrifuging the separated whey fraction to further remove fat, casein aggregates, and cell debris to form a substantially clear whey fraction; microfiltering the substantially clear whey fraction to remove remaining cell debris; centrifuging the microfiltered whey fraction to obtain a precipitate containing exosomes; incubating the exosome-containing precipitate in an aqueous medium to dissolve the precipitate without disrupting the exosome membranes and provide an exosome suspension; and drying the suspension to obtain the powdered exosomes. A method for reducing the risk of developing or treating insulin resistance, prediabetes, or diabetes in a subject utilizes exosomes isolated from bovine milk.

The invention relates to the technical field of mRNA vaccine preparation, and particularly discloses a porcine epidemic diarrhea virus mRNA vaccine and a preparation method thereof. The mRNA vaccine is prepared by adopting cow milk source exosome as a carrier and wrapping mRNA encoding PEDV-S protein in the carrier through an ultrasonic loading method. According to the preparation method, the encapsulation efficiency of mRNA and the stability of the vaccine are remarkably improved, the prepared vaccine can more efficiently promote antigen expression, stronger cellular immunity and humoral immunity are induced in a mouse body, and a safe and efficient novel vaccine candidate is provided for preventing and controlling porcine epidemic diarrhea.

This invention relates to an endogenous antimicrobial peptide from bovine milk and its applications. The antimicrobial polypeptide compound VAVALARPKHPIK is derived from endogenously enzymatically hydrolyzed bovine casein, with the amino acid sequence Val-Ala-Val-Ala-Leu-Ala-Arg-Pro-Lys-His-Pro-Ile-Lys. It exhibits broad inhibitory activity against both Gram-positive and Gram-negative bacteria, including *Porphyromonas gingivalis*, *Escherichia coli*, and *Staphylococcus aureus*. This antimicrobial peptide shows promising applications as a health supplement and / or medical device or pharmaceutical for the prevention and / or reduction of infectious diseases, as a food antimicrobial agent, in novel functional foods, pet food and animal feed additives, and in daily chemical products.

This invention discloses a desensitized milk peptide, its preparation method, and its application, relating to the food processing field. The preparation method provided by this invention includes: (1) removing the upper layer of fat from fresh milk; (2) circulating treatment under dynamic high-pressure microjet pressure; (3) adding mixed proteases for enzymatic hydrolysis; (4) adding mulberry polyphenols and using pulsed electric field combined with glycosylation modification to obtain a desensitized milk peptide solution; (6) adding sweeteners, stabilizers, and emulsifiers, mixing with the desensitized milk peptide solution, and then homogenizing in two stages to obtain a homogenized desensitized milk peptide solution; the obtained milk peptide solution can be sterilized and aseptically filled to obtain a desensitized milk peptide beverage. This invention hydrolyzes milk protein into polypeptides through enzymatic hydrolysis and pulsed electric field combined with glycosylation modification of whole milk, and then performs desensitization treatment, significantly reducing the allergenicity of milk, thereby ultimately producing a desensitized milk peptide beverage rich in milk peptides.

Fat blend comprising (i) 20-65 wt % of a vegetable lipid source and (ii) 35-80 wt % of a milk fat composition comprising at least one bovine milk fat fraction, wherein the milk fat composition comprises, relative to total fatty acid moieties, 37-48 mol % of long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0) and wherein 28-43 mol % of all fatty acid moieties positioned on the sn-1,3 position of the glycerol backbone in said milk fat composition are long chain saturated fatty acid moieties with 12-18 carbon atoms (LCSFA C12:0-C18:0).

Milk quality can be compromised by physiological conditions affecting dairy mammals, notably mastitis. Mastitis is an inflammatory disease of the mammary gland that may pose significant health and economic challenges. To address this, a system is proposed for monitoring at least one mammal to predict the onset of a clinical condition, such as, for example, mastitis. The system may be configured to sense one or more characteristics descriptive of mammal-related information, receive sensor data corresponding to those characteristics, and process the data to forecast the onset of mastitis within an upcoming time interval. Early detection enables timely intervention, promoting animal welfare and preserving milk quality.

The present invention relates to a novel vegan plant-based composition for preparing highly functional plant-based milk. The vegan plant-based composition comprises at least one base ingredient in a range of 10-99% by total weight of the vegan plant-based composition; and at least one functional ingredients in a range of 1-15% by total weight of the vegan plant-based composition, and optionally an additional ingredients in a range of 0.1-72% by total weight of the vegan plant-based composition. The vegan plant-based composition is shelf-stable at room temperature for over six months, without the requirement of preservatives, thus eliminating the requirement of the cold chain. The plant-based milk prepared in the present invention using the vegan plant-based composition is highly functional, having highly similar functional properties as that of bovine milk, taste-neutral, provides a creamy mouthfeel, heat stable, and acid stable when used for preparing strong tea and coffee.

The application provides a method for separating alpha s Casein, and belongs to the technical field of protein separation. The method takes bovine milk casein as raw material, separates a first precipitate mainly containing calcium-sensitive casein through low-temperature continuous phase change, and then redissolves the first precipitate, controls the feed liquid ratio during redissolution and the standing time in a low-temperature alkaline environment, maximally dissolves beta-casein, and thus obtains a precipitate rich in alpha s Casein. The method has high purity and yield of alpha s Casein, and the purity and yield of alpha Casein are 69.17%-88.93% and 81.96%-91.15% respectively.

The invention provides a method for separating alpha s-casein, and belongs to the technical field of protein separation. According to the method, bovine milk casein is used as a raw material, a first precipitate mainly containing calcium-sensitive casein is separated out through low-temperature continuous phase change and then redissolved, beta-casein is dissolved out to the maximum extent by regulating and controlling the material-liquid ratio during redissolving and the standing time in a low-temperature alkaline environment, and thus a precipitate rich in alpha s-casein is obtained; the purity of the high-purity alpha s-casein obtained by the method is 69.17%-88.93%, and the yield of the high-purity alpha s-casein obtained by the method is 81.96%-91.15%.

The invention provides casein phosphopeptides with high osteogenic activity as well as a preparation method and application thereof, and belongs to the technical field of active peptides. According to the method, bovine milk casein is taken as a raw material, calcium-sensitive protein is separated out through low-temperature continuous phase change, then the calcium-sensitive protein is redissolved to obtain supernate, the supernate is dried to obtain enriched beta-casein, and the enriched beta-casein is prepared into casein phosphopeptides through an enzymolysis method. Casein with the market price lower than 200 yuan / kg is used as a raw material, and the simple preparation method reduces the dosage of low-cost reagents, so that the preparation cost of the casein phosphopeptides is greatly reduced. The preparation cost of the casein phosphopeptides is greatly reduced. Moreover, the beta-CPPs prepared by the invention has high osteogenic activity, and compared with CPPs, the beta-CPPs can more effectively promote proliferation, differentiation and mineralization of osteoblasts at low concentration, so that the preparation cost of the CPPs is further reduced. In addition, the preparation method provided by the invention is high in controllability, simple and convenient to operate, capable of realizing efficient and stable production of CPPs, and suitable for industrial large-scale preparation.

The invention relates to the technical field of nutritional foods, and particularly discloses a nutritional composition for regulating sleep as well as a preparation method and application of the nutritional composition. The nutritional composition is prepared from cow milk exosome, 1, 3-dioleic acid 2-palmitic acid triglyceride and 1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride. The nutritional composition provided by the invention can promote the expression level of gamma-aminobutyric acid receptors and melatonin so as to achieve the purposes of improving sleep, assisting in regulating circadian rhythm and increasing deep sleep proportion, and a new idea is provided for preparing nutritional foods for regulating sleep.

The invention relates to a fat composition and a nutritional composition comprising such fat composition. The nutritional composition is particularly suitable for therapeutic applications in treating gut discomfort and / or constipation as well as in non-therapeutic applications for reducing the intestinal formation of calcium and magnesium fatty acid soaps. The fat composition comprises a mixture of triacylglycerols (TAG) originating from a bovine milk fat source and a vegetable lipid source, said mixture being characterized by: (a) a content of butanoate groups (C4:0) of 0.5-2.8% by weight based on total weight of fatty acid acyl groups in TAG; (b) a wLCSFA(sn-1,3) of 18.0-35.0% by weight; (c) a mLCFA (sn-1,3) of 48.0-61.0 mol %; and (d) a ratio mLCFA (sn-1,3) / SFA of 0.70-1.25, wherein: —LCFA(sn-1,3) are the long chain fatty acid acyl groups having a chain length of 12 or more carbon atoms at the sn-1 and sn-3 position in TAG; —LCSFA(sn-1,3) are the long chain saturated fatty acid acyl groups having a chain length of 12 or more carbon atoms at the sn-1 and sn-3 position in TAG; —wLCSFA(sn-1,3) is the amount of LCFA(sn-1,3) in % by weight based on total weight of fatty acid acyl groups in TAG; —mLCFA(sn-1,3) is the mole fraction of LCFA(sn-1,3) based on total moles of fatty acid acyl groups in the TAG as expressed in mol %; and—SFA is the mole fraction of saturated fatty acid acyl groups in the TAG as expressed in mol %.

The present invention discloses an antioxidant stress preparation, a preparation method and an application thereof, and belongs to the field of food technology. The antioxidant stress food provided by the present invention is prepared by mixing and drying bovine milk exosomes, microalgae, probiotics and cationic polysaccharides that embed active antioxidants. The antioxidant stress food provided by the present invention has good biosafety. The food can not only delay aging caused by oxidative stress and alleviate neurodegenerative diseases, but also has the functions of lowering blood sugar and blood lipids. It can be used as a functional food or a functional food additive for the efficient prevention and treatment of neurodegenerative diseases and liver diseases related to oxidative stress.

The invention belongs to the technical field of dairy product detection, and relates to a characteristic peptide fragment for identifying alpha-casein of camel milk, a qualitative identification method and application. The characteristic peptide fragment comprises a characteristic peptide fragment of cow milk alpha-casein, and the characteristic peptide fragment of the cow milk alpha-casein is CAGCPHCPDHWMGYGDHCYYFSVEK or EYSTAHLSRTLTLVEQIK; the characteristic peptide fragment of the cow milk alpha-casein is CAGCPHCPDHWMGYGDHCYYFSVEK; the characteristic peptide fragment of the camel milk alpha-casein is PLHYLTIINPKK. According to the method, the characteristic peptide fragment of the alpha-casein of the camel milk is screened by combining enzymolysis, high performance liquid chromatography and high-resolution mass spectrometry, and then the characteristic peptide fragment is used for qualitatively identifying the camel milk, so that the accuracy of an identification result is high; further, the identification of the adulterated cow milk in the camel milk is realized.

The present invention belongs to the field of biomedical materials, and more particularly relates to a composite modified hydrogel formulation and its preparation method. This composite modified hydrogel formulation comprises the following raw materials, calculated by weight: 0.8-1.5% bovine milk lipid microspheres grafted with hyaluronic acid, 0.1-0.3% modified magnesium-aluminum layered double hydroxide, and the balance deionized water. This composite modified hydrogel formulation exhibits high drug loading and excellent sustained-release properties.

A method of promoting healthy catch-up growth in a pediatric individual comprises administering a nutritional composition with an exosome-enriched product comprising intact bovine milk-derived exosomes to the pediatric individual during a period of weight gain. A method also provides promoting healthy catch-up growth in an underweight individual by administering an exosome-enriched product comprising intact bovine milk-derived exosomes to the individual.

The present invention discloses a plasmin-inhibiting peptide YHX-PIP-4 derived from bovine milk protein and its application, and relates to the technical field of bioactive peptides. The present invention provides a plasmin-inhibiting peptide YHX-PIP-4 derived from β-lactoglobulin, whose amino acid sequence is shown in SEQ ID NO.1. With the help of bioinformatics technology, the present invention screened a peptide segment with plasmin inhibition ability from the β-lactoglobulin sequence through molecular docking and molecular dynamics simulation technology. This peptide segment is named plasmin-inhibiting peptide YHX-PIP-4; studies have confirmed that the plasmin-inhibiting peptide YHX-PIP-4 can inhibit the activity of plasmin, and adding it to ultra-high temperature milk can avoid the precipitation and aging gel phenomenon of milk during storage, thereby extending its shelf life.

The invention relates to the technical field of molecular biology, in particular to cow milk extracellular vesicles as well as an extraction process and application thereof. The extraction process of the cow milk extracellular vesicles comprises the following steps: S1, centrifugally separating cow milk to obtain a precipitate, and re-suspending the precipitate by adopting a buffer solution to obtain a suspension solution; s2, establishing a sucrose density gradient layer by using sucrose solutions with the mass percentage concentrations of 60%, 45%, 30%, 15% and 8% respectively, and performing density gradient centrifugation on the suspension solution obtained in the step S1 by using the sucrose density gradient layer; and S3, collecting a sample between the 30% sucrose layer and the 45% sucrose layer, washing the sample, and carrying out ultrafiltration purification to obtain the cow milk extracellular vesicles. The yield and the purity of the cow milk extracellular vesicles can be effectively improved.

This invention relates to the field of nutritional food technology, specifically disclosing a nutritional composition for regulating sleep, its preparation method, and its application. The nutritional composition comprises bovine milk exosomes, 1,3-dioleoyl-2-palmitoylglycerol, and 1-oleoyl-2-palmitoyl-3-linoleic acid triglyceride. The nutritional composition provided by this invention can promote the expression levels of γ-aminobutyric acid receptors and melatonin, thereby improving sleep, assisting in the regulation of circadian rhythms, and increasing the proportion of deep sleep. This invention provides a new approach for preparing nutritional foods that regulate sleep.

This invention belongs to the field of bioactive peptide technology, and relates to a plasmin inhibitory peptide derived from β-lactoglobulin, its screening method, and its application. Its amino acid sequence is shown in SEQ ID NO.1. This invention selects β-lactoglobulin from bovine milk as the source of the plasmin inhibitory peptide. Using the structure of the naturally conformated μ-plasmin as a template, molecular docking is performed between β-lactoglobulin and plasmin. Molecular dynamics simulations are conducted on the complex until equilibrium is reached. Based on the interaction between the active site of plasmin and the β-lactoglobulin sequence, targeted screening is performed on polypeptide sequences within the β-lactoglobulin sequence that may have inhibitory activity, resulting in a small, short peptide that specifically inhibits plasmin and can be rapidly synthesized artificially. Adding this plasmin inhibitory peptide to UHT milk increases the stability of sterilized milk and extends its shelf life, showing promising application prospects.

This application relates to the field of dairy processing technology, specifically disclosing a composite DHA milk protein peptide, its preparation method, and its application. The process involves homogenizing DHA algal oil with an emulsifier under high pressure to prepare a nano-sized DHA emulsion (particle size 100-300 nm), achieving efficient encapsulation and stabilization protection of DHA. The resulting DHA emulsion is then mixed with skim milk, and targeted enzymatic hydrolysis with trypsin and flavor protease at 45-55℃ and pH 6.5-7.0 for 2-4 hours hydrolyzes the milk protein into small molecule peptides. After enzyme inactivation and drying, the composite DHA milk protein peptide is obtained. This process significantly improves protein digestibility while protecting DHA stability; in vitro simulated digestion experiments show a digestibility and absorption rate of over 94.8%. Further application of this protein peptide in the preparation of liquid milk yields a DHA retention rate of over 92%, and even after 6 months of storage at room temperature, there is no obvious oxidized fishy odor. This effectively solves the technical problems of DHA's easy oxidation and poor stability in dairy products, demonstrating good nutritional synergistic value and promising industrial application prospects.

The invention provides a preparation method of an aflatoxin M2 matrix standard substance in freeze-dried cow milk, which is characterized by comprising the following steps: S1, adding an aflatoxin M2 standard substance into the cow milk until the concentration of aflatoxin M2 in the cow milk is 0.5 mu g / kg, slowly stirring and uniformly mixing, and sub-packaging into clean penicillin bottles according to parts; s2, pre-freezing at-20 DEG C for 24 hours, and then carrying out vacuum freeze-drying on the penicillin bottle filled with the frozen cow milk; s3, after freeze-drying the cow milk, covering the cow milk with a cover, and storing the cow milk at-20 DEG C; s4, performing uniformity test and stability test on the freeze-dried matrix standard substance; and S5, cooperatively valuing the prepared matrix standard substance in combination with a plurality of laboratories, calculating uncertainty, and finally determining the characteristic value of the aflatoxin M2 matrix standard substance in the freeze-dried cow milk. The standard substance disclosed by the invention has good uniformity and stability, and can meet the requirement of providing the standard substance required by method verification and quality control for detecting aflatoxin M2 in cow milk.

This invention belongs to the field of bioactive peptide technology, and relates to a β-lactoglobulin-derived plasmin inhibitory peptide, its screening method, and its application. The amino acid sequence of the plasmin inhibitory peptide is shown in SEQ ID NO.1. The screening method involves selecting β-lactoglobulin from bovine milk as the source of the plasmin inhibitory peptide. After preliminary verification that β-lactoglobulin has the ability to inhibit plasmin, molecular docking is performed on β-lactoglobulin and plasmin. The complex is then subjected to molecular dynamics simulation to equilibrium. Based on the interaction between the active site of plasmin and the β-lactoglobulin sequence, targeted screening is conducted on polypeptide sequences in the β-lactoglobulin sequence that may have inhibitory activity. This method solves the technical defects in the current preparation process of plasmin inhibitory peptides, such as the scarcity of raw materials, complex processes, and difficulty in large-scale production. This plasmin inhibitory peptide increases the stability of sterilized milk and extends its shelf life, showing good application prospects.

The invention provides hypoallergenic eye-protecting children milk tablets and a preparation method thereof, and the hypoallergenic eye-protecting children milk tablets comprise hypoallergenic formula whey protein powder, casein hydrolysate freeze-dried powder, eye-protecting composite active ingredients, a flavoring agent, an antioxidant and auxiliary materials, wherein the low-sensitization formula whey protein powder comprises a mixture of lactoferrin powder, alpha-lactalbumin powder and low-sensitization beta-LG protein powder, and the low-sensitization beta-LG protein powder comprises a non-covalent compound formed by combining a small molecule substance and cow milk beta-LG; the casein hydrolysate freeze-dried powder comprises bovine milk casein hydrolyzed by Alcalase protease and bacillus licheniformis protease. The selected small molecule substances are all dairy product available additives permitted by GB2760-2024 National Food Safety Standard Food Additives Usage Standards, and related reports of the food additives for beta-LG sensitization improvement do not exist in the existing research at present, so that the blank in the prior art is filled, and the eating safety of children is guaranteed.

A method for improving bone formation in an individual comprises administering a bovine milk exosome-enriched product and vitamin K2 to the individual. A method for reducing a risk of bone fracture or strengthening bone in an individual comprises administering a bovine milk exosome-enriched product and vitamin K2 to the individual. A method for preventing or delaying onset or development of osteoporosis in an individual comprises administering a bovine milk exosome-enriched product and vitamin K2 to the individual.

The invention discloses a process for extracting high-purity lactoferrin and lactoperoxidase from fresh milk, which comprises the following steps: centrifugally degreasing the fresh milk to obtain skimmed milk; sterilizing and concentrating the obtained skimmed milk through a ceramic membrane to obtain sterilized concentrated milk liquid; adsorbing and eluting the sterilized concentrated milk liquid through cation exchange resin, and collecting eluent rich in lactoferrin and lactoperoxidase; desalting and concentrating the obtained eluent through a ceramic membrane to obtain secondary concentrated milk liquid; the obtained secondary concentrated milk liquid is introduced into a simulated moving bed chromatography system, a lactoferrin extracting solution is obtained from an extracting solution outlet of the simulated moving bed chromatography system, and a lactoperoxidase extracting solution is obtained from a raffinate outlet; carrying out desalination and concentration by using an ultrafiltration membrane, and carrying out spray drying to obtain a lactoferrin final product and a lactoperoxidase final product; the purity of lactoferrin in the obtained lactoferrin final product is 95% or above, and the purity of emulsified peroxidase in the obtained lactoperoxidase final product is 90% or above.

The present application relates to a kind of bovine milk endogenous antibacterial peptide and its application, it is from endogenous enzyme hydrolysis casein of antibacterial polypeptide compound PKTKVIPYVRYL of milk, its amino acid sequence is Pro-Lys-Thr-Lys-Val-Ile-Pro-Tyr-Val-Arg-Tyr-Leu.Gram-positive bacteria and gram-negative bacteria have extensive inhibitory activity respectively, including gingival porphyromonas, escherichia coli, staphylococcus aureus etc..The antibacterial peptide can be used as the health care product and / or medical equipment or drug, food bacteriostatic agent, new functional food, pet food and animal feed additive and daily chemical in preventing and / or reducing infection disease, with good application prospect.