41 results about "Immunologic Competence" patented technology

The invention discloses an aptamer and a new use of derivative thereof. The new use provided by the invention is application of aptamer and the derivative thereof in preparation of target drugs. The invention further protects the target drugs obtained by coupling the drugs with the aptamer or the derivative thereof. The invention has the advantages of screening and preparing specific aptamer or derivative thereof under the condition of not knowing the molecular transformation of the target cell surface related to the disease, and can simultaneously obtain multiple aptamers or derivatives thereof of molecular related to the cell surface cell; the specificity of the obtained aptamer or derivative thereof is strongly combined with the disease cell; the aptamer or the derivative thereof can be chemically synthesized in large scale and can be easily connected with the drug molecules and has low cost; the molecular weight of the aptamer or the derivative thereof is relatively small without immunologic competence and toxicity; the aptamer or the derivative thereof has good stability; and the formed target drugs are stable. The invention has very high application value.

The invention provides a streptococcus thermophilus AR-333 strain and application thereof. The streptococcus thermophilus AR-333 strain is obtained through being separated from naturally fermented milk, and the collection number of the strain is CGMCC 10262. The streptococcus thermophilus AR-333 is fermented for 16h under the condition of 40 DEG C by skimmed milk; the yield of extracellular mucopolysaccharide reaches 256.97 mg / L; meanwhile, the streptococcus thermophilus is used as a yoghourt fermenting agent; the viscosity of yoghourt can be obviously improved; the tissue state of the yoghourt can be improved; good flavor is realized; the generated extracellular mucopolysaccharide has an immunologic competence regulating function; macrophages RAW264.7 can be simulated to release NO.

The invention relates to a method for preparing microbial feed additive used for promoting growth of goose liver. All of the microbial strain is screened out from the animal intestinal tract. The method comprises culturing all the strains on the same culture medium through combined culture, constituting powerful compound microbial community by using mixed sync solid fermentation process, and forming effective enzyme producing ability to make the product has amount of physiologic metabolite such as organized enzyme for analysing cellulose and fructose, peptide, antioxidants, free amino acid, and vitamin. The invention can promote feed digestion and absorption of animal, promote synthesize and utilization of protein, enhance immunologic competence of livestock, fowl and aquatic livestock, and improve feed conversion ratio and production capacity of breed animal. The inventive product has good practicability and pertinency.

The invention relates to bursa of Fabricius heptapeptide with immune regulation effect and application thereof in immunity (in improving the immune capability of animals, improving the immune effect of vaccines and affecting the activity of tumor cells), and belongs to the field of immunology. The molecular weight of the separated heptapeptide is 722.240, the amino acid sequence is EPASGMM, and the heptapeptide has a simple structure, no toxic or side effect and extremely weak immunogenic property. The heptapeptide can be separated and extracted from bursa of Fabricius, also can be chemically synthesized, has low cost, and can be massively produced. The bursa of Fabricius heptapeptide has induction effect on the production of antibody and subtype thereof, and meanwhile can regulate the production of cell factors, partition of T lymphocytes and proliferation of spleen cells, and promote the immune reaction of the cells. The bursa of Fabricius heptapeptide is an immune regulation factor on functions, has wide application prospect on the aspects of immune regulation, immune therapy and the like, and can be applied in the fields of basic immune research, clinical application research and the like.

This document relates to methods and materials involved in evaluating immune competence in a subject. For example, methods and materials for evaluating immune competence in a subject to BK virus (BKV) are provided. Also provided are isolated peptides, compositions, and methods useful for evaluating whether or not a subject is BKV immunocompetent. Also provided are methods for treating BK virus nephropathy in a subject that is BKV immune incompetent.

Improved methods, reagents, and kits for quantitation of HLA-DR and / or CD11b expression on peripheral blood cells are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR and CD11b expression. Use of a novel anti-CD14 conjugate, anti-CD14-PerCP / CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits may be used to assess immune competence, and to direct and monitor immunostimulatory therapies in septic patients exhibiting monocyte deactivation.

The invention discloses bamboo leaves teabag and a preparation process and is made of the following raw material according to the following weight percentage: tender tea leaves 60-80 percent and tender bamboo leaves 20-40 percent. Procedure is as follows: tender tea leaves is cut into fine-grained share with drying, sterilization, strip straightening out and rolling and cooling; with cleaning and drying as well as hot steaming, rolling and baking at a certain temperature and for a certain time, bamboo leaves is cut into fine-grained share; after processed with the prodecures, tender tea leaves and tender bamboo leaves is mixed together by a certain ratio and then is packaged after repeat stirring and sterilization. Bamboo leaves teabag provided by the invention is convenient and time-saving while having the good health care effect of conditioning visceral balance and strengthening immunologic competence and no preservative.

The invention relates to a method for the preparation of efficient antigen-presenting human γδ T cells, to the γδ T cells prepared by such a method, and to their use in immunotherapy, vaccination, vaccine development and diagnostics. Similar to dendritic cells (DCs) in potency and efficacy, these human γδ T cells process antigens and present antigenic peptides to αβ T cells and induce antigen-specific responses (proliferation and differentiation) in naïve αβ T cells. γδ T cells are easily purified from peripheral blood, acquire “maturation” status (expression of essential adhesion, co-stimulatory and major histocompatibility complex molecules) within 1 day of in vitro culture under stimulation and induce strong primary and secondary T helper cell responses. The γδ T cells may be used in a method of treatment of tumors or chronic or recurrent infectious diseases, in identification of novel tumor or pathogen-derived antigens, and in the diagnosis of the immune competence of a patient.

The invention relates to a vitamin premix for juvenile sea cucumbers and a preparation method thereof. The invention is characterized in that the vitamin premix per ton comprises 500 thousand IU to 1000 thousand IU of vitamin A, 100 thousand IU to 500 thousand IU of vitamin D, 5g to 10g of vitamin E, 1g to 5g of vitamin K3, 1g to 5g of vitamin B1, 1g to 5g of vitamin B2, 1g to 5g of vitamin B6, 1g to 5g of vitamin B12, 0.5g to 15g of vitamin C, 5g to 10g of nicotinic acid, 100mg to 500mg of folic acid, 5g to 10g of pantothenic acid, 10g to 15g of inositol, 1g to 5g of calcium pantothenate, 10mg to 20mg of biotin and the balanced alpha-cellulose. The preparation method comprises the following steps: firstly weighting the vitamin D, the vitamin B12, the folic acid, the biotin and 100g of alpha-cellulose and mixing the same evenly; then, adding the other weighted vitamins and 500g of alpha-cellulose and mixing evenly the mixture; and finally, adding the rest alpha-cellulose to the mixture and mixing the new mixture to be fully even. The invention has the advantages that the growth performance and the immunologic competence of the juvenile sea cucumbers in a growth period are remarkably improved, the addition effect is stable, the cost is low and the application is convenient.

The invention relates to a method for the preparation of efficient antigen-presenting human γδ T cells, to the γδ T cells prepared by such a method, and to their use in immunotherapy, vaccination, vaccine development and diagnostics. Similar to dendritic cells (DCs) in potency and efficacy, these human γδ T cells process antigens and present antigenic peptides to αβ T cells and induce antigen-specific responses (proliferation and differentiation) in naïve αβ T cells. γδ T cells are easily purified from peripheral blood, acquire “maturation” status (expression of essential adhesion, co-stimulatory and major histocompatibility complex molecules) within 1 day of in vitro culture under stimulation and induce strong primary and secondary T helper cell and cytotoxic T cell responses. The γδ T cells may be used in a method of treatment of tumors or chronic or recurrent infectious diseases, in identification of novel tumor or pathogen-derived antigens, and in the diagnosis of the immune competence of a patient.

The invention relates to a feed additive, in particular to a method for preparing fructo-oligosaccharide serving as a feed additive with an enzyme method, belonging to the technical field of deep processing of farm products. Fructo-oligosaccharide is a nuisanceless and functional dual-purpose feed additive which can be used for stimulating the growth of bifidobacteria, suppressing the growth of diarrhea causing pathogenic bacteria, eliminating or reducing the possibilities of specific recognition and binding of pathogenic bacteria with intestinal wall cells of a host animal by being bound with specific glycosyl binding sites of enteric pathogenic bacteria, reducing the settling and reproduction of salmonella, colon bacillus and enterococcus in intestinal tracts, inducing the production of microbial polysaccharide carbohydrate hydrolase in alimentary tracts of animals and directly or indirectly stimulating in-vivo immunologic competence of animals positively, and the like. By applying the fructo-oligosaccharide to a feed additive, the feed conversion rate and the health levels of animal organisms can be increased remarkably, a part of conventional antibiotics for feeds are replaced, the residual quantity of antibiotics in livestock products is reduced, the quality of livestock products is improved, and the growing speed of animals is increased remarkably.

The present disclosure relates to a Fusobacterium nucleatum FomA protein vaccine, a preparation method and applications thereof. The preparation method comprises: linking a signal-peptide-sequence-deleted FomA gene linked to a MBP tag to an expression vector, transforming into Escherichia coli, carrying out induced expression, carrying out affinity purification on FomA recombinant protein by usingAmylose Resin High Flow, and carrying out enzyme digestion with a tev enzyme to remove the MBP tag to obtain the Fusobacterium nucleatum FomA protein vaccine. According to the present invention, theFusobacterium nucleatum FomA protein vaccine has good stability, and can effectively improve the immunity of organisms to Fusobacterium nucleatum, improve the survival rate and delay the occurrence and development of colons tumor; and in APCMin / + mice models, the FomA protein vaccine has significant protecting effect on the mice, can be used as a new colon tumor vaccine candidate, and has great application prospects.

The present invention relates to a method for treatment of cancer, which comprises the following steps (A) and (B): (A) applying a treatment which induces the reduction in lymphocytes to a patient; and (B), subsequent to step (A), administering lymphocytes to the patient promptly. The present invention also relates to a cancer therapeutic agent and a cancer treatment kit for use in the method. When the method is used as an immunoreconstructive therapy, the decrease in immunologic competence which may be caused by the reduction in lymphocytes can be avoided and, therefore, the risk of the occurrence of an infectious disease can be decreased.

The invention belongs to the technical field of fodder, and particularly relates to a material for preventing bovine gastrointestinal nematodiasis and a preparation method of the material. According to the material, a traditional Chinese medicine preparation, stillage, phoenix tree leaves, corn flour, wheat bran, eggshell powder and salt are taken as raw materials, and are reasonably matched to prepare the material for preventing the bovine gastrointestinal nematodiasis. The material for preventing the bovine gastrointestinal nematodiasis can promote the growth and development of cows, has an obvious weight increment effect, and can lower the morbidity of the bovine gastrointestinal nematodiasis simultaneously, so that the bovine immunologic competence is improved, and the economic loss of farmers is reduced.

The invention discloses preparation and application of a recombinant yeast preparation for co-expression of porcine interleukin 4 / 6 and fusion porcine antibacterial peptide. The antibacterial peptidefusion cytokine FFPIL46 is as follows A1), A2) or A3); A1) is the protein of which an amino acid sequence is the sequence 1 in a sequence listing; A2) is the protein with the same functions as the amino acid sequence shown by the sequence 1 in the sequence listing subjected to substitution and / or deletion and / or addition of one or more amino acid residues; A3) is the fusion protein obtained by connecting the N-terminal or / and C-terminal of A1) or A2) with labels. Experiments show that the FPAPL46 can promote the proliferation of lymphocytes, red blood cells and white blood cells, inhibit the growth of pathogenic microorganisms, promote the secretion of non-specific antibodies (IgG, IgG1, IgG2a) and disease-specific antibodies, and improve the immunity and survival rate of animals.

The invention relates to a method for the preparation of efficient antigen-presenting human γδ T cells, to the γδ T cells prepared by such a method, and to their use in immunotherapy, vaccination, vaccine development and diagnostics. Similar to dendritic cells (DCs) in potency and efficacy, these human γδ T cells process antigens and present antigenic peptides to αβ T cells and induce antigen-specific responses (proliferation and differentiation) in naïve αβ T cells. γδ T cells are easily purified from peripheral blood, acquire “maturation” status (expression of essential adhesion, co-stimulatory and major histocompatibility complex molecules) within 1 day of in vitro culture under stimulation and induce strong primary and secondary T helper cell and cytotoxic T cell responses. The γδ T cells may be used in a method of treatment of tumors or chronic or recurrent infectious diseases, in identification of novel tumor or pathogen-derived antigens, and in the diagnosis of the immune competence of a patient.

Immunocompetent animal models having immunological tolerance to a xenograft and methods of producing the same. The animal models are tolerized to the xenograft in the pre-immune, fetal or embryonic stage, followed by transplanting the xenograft into the animal in the post-natal stage, such that the post-natal animal is immunologically tolerant to the xenograft, while remaining immunocompetent.

The invention discloses preparation for a genetic modified rice formulation and usage thereof. The preparation includes steps of: A. acquiring specific promoter for rice and semaphore peptides thereof; B. obtaining human GM-CSF gene of rice's genetic codon; C. genetically transforming rice gene and highly expressing selection of genetic modified plant; D. processing seeds of genetic modified rice. An application of genetic modified rice seed as medicament for treating or preventing leucocytopenia is also disclosed. The invention is simple in process, low in cost, good in safety property and curative effect, and has no side effect. Because recombinant rhGM-CSF is stored in rice flour, and rice is main food of human, any purification steps are not needed in tablet preparation, and the tablet contains no pathogenic bacteria and virus of human, the invention is safe and reliable. The tablet has functions to stimulate proliferation of leukocyte, improve immunologic competence in vivo, recover lowered immunologic competence resulted by reduction of leucopenia induced by chemotherapy and radiotherapy of cancer.

The invention discloses the preparation and application of an antimicrobial peptide fusion cytokine CAMPILs co-expression biological preparation. The antimicrobial peptide fusion cytokine CAMPILs provided by the present invention is as follows A1), A2) or A3): A1) the amino acid sequence is the protein of sequence 1 in the sequence listing; A2) the amino acid sequence shown in sequence 1 in the sequence listing is passed through a or several amino acid residue substitutions and / or deletions and / or additions and proteins with the same function; A3) A fusion protein obtained by linking tags at the N-terminal or / and C-terminal of A1) or A2). Experiments have proved that CAMPILs of the present invention can promote the proliferation of lymphocytes, red blood cells and white blood cells, inhibit the growth of pathogenic microorganisms, promote the secretion of non-specific antibodies (IgG, IgG1, IgG2a) and disease-specific antibodies, and improve the immunity and survival of animals. Rate.

The invention relates to a liquid dairy product containing omega-3 unsaturated fatty acid; 0.01-10 parts of omega-3 unsaturated fatty acid are contained per 100 parts by weight. Because omega-3 unsaturated fatty acid is reasonably added, the dairy product has good stability and taste while providing the immunologic competence efficacy. The invention also relates to the production method of the liquid dairy product; omega-3 unsaturated fatty acid is added in sterilized normal temperature posterior segment so that all functional components will not lose and the health-care function can be fully performed.

The invention discloses a streptococcus pyogenes HtsA protein vaccine as well as a preparation method and an application thereof. The streptococcus pyogenes vaccine is prepared by the following operations: firstly, linking an HtsA gene that a signal peptide sequence is removed to an expression vector containing a His tag; then, transferring escherichia coli, implementing induced expression, and implementing Ni-affinity chromatography purification on HtsA recombinant protein; and finally, conducting mixed emulsifying of the HtsA recombinant protein and an immunologic adjuvant, so that the streptococcus pyogenes vaccine is obtained. The vaccine is good in stability; the immunological capacity of an organism to GAS is effectively enhanced, and a survival rate is improved; titers of the HtsA protein and the immunologic adjutant to an HtsA antibody in serum undergoing immune response can reach 1 / 163840-1 / 2621440; and in a mouse bacteremia model and a skin infection model, the HtsA proteinvaccine, which shows an obvious protective effect on mice, can serve as a novel vaccine candidate of streptococcus pyogenes; therefore, the HtsA protein vaccine is relatively high in application prospect.

The invention discloses an active polypeptide composition and application thereof to animal vaccines. The composition includes Lys-His-Gly-NH2 and Salpichrolide O, is dedicated to improve the immunity of animals such as chickens, ducks, rabbits, pigs, sheep, dogs, cows, and deer, and other economic animals, and can improve the immune effect of the vaccine. The active polypeptide is a small-molecular-weight natural substance in the body of animals, is non-toxic in individual usage or combination with vaccines, does not produce antibodies against the active polypeptide. The active polypeptide improves immunity by improving the host's own immune response capability and is very safe and reliable. The active polypeptide is convenient and low-cost for chemical synthesis manufacturing, and the pharmaceutical grade active polypeptide is more secure and reliable. Salpichrolide O is also a natural material with small molecular weight, and is non-toxic in individual usage or combination with vaccines; and the Salpichrolide O cooperating with the active polypeptide can further improve the host's own immune response capability, thereby further enhancing immunity.

The invention provides a preparation method of fermented crystallized haws and an obtained product. The method comprises the steps: preparing cordyceps militaris fermentation liquor; carrying out sorting; carrying out sugar steeping; inoculating fermented base stock with the cordyceps militaris fermentation liquor according to the inoculum size of 15% to 20%, and carrying out fermentation for 32 to 48 hours at the temperature of 30 DEG C to 35 DEG C; carrying out stringing; carrying out sugar cooking; carrying out sugar dipping. The method provided by the invention is unique and is simple in process flow and convenient in operation, and the product is more unique in taste on the basis of reserving the basic color, shape and taste of the traditional crystallized haws; due to the fermentation action of cordyceps militaris, the shelf life of the product is prolonged; the product has the health-care function of improving immunologic competence of human bodies and the like.

The invention relates to a novel lactobacillus strain and a novel composition which contains an unknown strain which can be used for enhancing the anti-allergic capability. The composition can exist in the form of food or medicinal composition. A method for screening and testing the lactobacillus strain composition for enhancing the Th1 immunological competence and regulate overreacting Th2 immunologic reaction caused by allergy is also disclosed.

The invention discloses an instant coffee formula capable of slimming and detoxifying, and relates to the field of health drinks. The instant coffee formula capable of slimming and detoxifying uses Brazilian black coffee powder, L-carnitine, dietary fiber, xylooligosaccharide, vitamins, and compound enzyme powder For the raw material. The instant coffee can promote kidney function, excrete excess sodium ions in the body, increase urine output, improve abdominal distension and edema, and help lose weight. It has a protective effect on gastric ulcer, can promote the synthesis of DNA and protein, prolong the life of red blood cells, enhance the immunity of disease, and delay the aging of blood vessels and tissues. The instant coffee that can slim down and detoxify is used for socializing, people with irregular diet, obese people, and fatigued people, and long-term consumption can improve the situation.

The invention relates to bursa of Fabricius heptapeptide with immune regulation effect and application thereof in immunity (in improving the immune capability of animals, improving the immune effect of vaccines and affecting the activity of tumor cells), and belongs to the field of immunology. The molecular weight of the separated heptapeptide is 722.240, the amino acid sequence is EPASGMM, and the heptapeptide has a simple structure, no toxic or side effect and extremely weak immunogenic property. The heptapeptide can be separated and extracted from bursa of Fabricius, also can be chemically synthesized, has low cost, and can be massively produced. The bursa of Fabricius heptapeptide has induction effect on the production of antibody and subtype thereof, and meanwhile can regulate the production of cell factors, partition of T lymphocytes and proliferation of spleen cells, and promote the immune reaction of the cells. The bursa of Fabricius heptapeptide is an immune regulation factoron functions, has wide application prospect on the aspects of immune regulation, immune therapy and the like, and can be applied in the fields of basic immune research, clinical application research and the like.

The invention discloses feedstuff for enhancing mating capability of rams and a preparation method thereof. The feedstuff comprises the ingredients in percentage by weight: 60% to 80% of straws, 13% to 30% of maize, 2% to 4% of soybean meal, 1.5% to 2.5% of dicalcium phosphate, 0.2% to 0.5% of common salt, 0.5% to 2% of compound additives and 0.5% to 2.5% of traditional Chinese medicine additives,wherein the traditional Chinese medicine additives comprise the ingredients in parts by weight: 15-20 parts of semen juglandis, 12-15 parts of Chinese chive seeds, 10-12 parts of cortex phellodendri,10-12 parts of tangerine seeds, 9-12 parts of rhizoma dioscoreae, 8-12 parts of prepared rehmannia roots, 9-11 parts of aconite roots, 8-10 parts of cortex cinnamomi, 6-9 parts of fructus lycii and 6-8 parts of Chinese angelica. The feedstuff is ingenious in prescription and can be used for effectively improving semen collection amount and sperm density of the rams and enhancing the mating capability of the rams; the immunologic competence of the rams can be enhanced, and the physical constitution of propagated offspring is robust.

The invention discloses engineered yeast of a fish natural killer cell enhancer and an application thereof. The puffer fish NKEF engineered yeast is named as saccharomyces cerevisiae INVSC1-pYES2-NKEFwith the preservation number of CGMCC1847. The engineered yeast is obtained by inserting the puffer fish NKEF gene into the place between EcoRI and XhoI restriction enzyme cutting sites of a pYES2 carrier to construct a recombinant plasmid and then introducing the recombinant plasmid into the expression host saccharomyces cerevisiae. The engineered yeast is cultivated under the condition of 30 oC, induced by 2% galactose, and the expression of NKEF recombinant protein can reach more than 20%. The anti-disease application of the above-mentioned engineered yeast in aquatic animal breeding is also disclosed, and the effect of improving the anti-infective capability and autoimmune capability of aquaculture animals can be achieved.

The present invention belongs to the field of medicine, and specifically discloses an amino alcohol derivative represented by Formula I, a pharmaceutically acceptable salt, solvate, polymorph or prodrug thereof. In addition, the present invention also discloses a pharmaceutical composition comprising the above substances, and a use of the substance in the preparation of a medicament for the prevention and treatment of an immune inflammatory disease, or a disease or condition associated with immunological competence such as multiple sclerosis, ALS, CIDP, systemic lupus erythematosus, rheumatoid arthritis, ulcerative colitis, psoriasis, polymyositis, etc.

The invention discloses preparation and application of a fusion bovine antibacterial peptide (FBAP) recombinant yeast preparation. The invention provides four kinds of bovine antibacterial peptides; amino acid sequences of the four kinds of bovine antibacterial peptides are 1-12 sites of a sequence 1, 21-32 sites of the sequence 1, 41-55 sites of the sequence 1 and 63-75 sites of the sequence 1 respectively. Proved by experiments, the FBAP provided by the invention can promote proliferation of lymphocytes, erythrocytes and leukocytes, inhibits growth of pathogenic microorganisms, promotes secretion of non-specific antibodies (IgG, IgG1 and IgG2a) and disease-specific antibodies, improves the immunity of animals and increases the survival rate of the animals.