41 results about "Immunologic Competence" patented technology

The invention relates to a method for the preparation of efficient antigen-presenting human γδ T cells, to the γδ T cells prepared by such a method, and to their use in immunotherapy, vaccination, vaccine development and diagnostics. Similar to dendritic cells (DCs) in potency and efficacy, these human γδ T cells process antigens and present antigenic peptides to αβ T cells and induce antigen-specific responses (proliferation and differentiation) in naïve αβ T cells. γδ T cells are easily purified from peripheral blood, acquire “maturation” status (expression of essential adhesion, co-stimulatory and major histocompatibility complex molecules) within 1 day of in vitro culture under stimulation and induce strong primary and secondary T helper cell and cytotoxic T cell responses. The γδ T cells may be used in a method of treatment of tumors or chronic or recurrent infectious diseases, in identification of novel tumor or pathogen-derived antigens, and in the diagnosis of the immune competence of a patient.

The invention discloses the preparation and application of an antimicrobial peptide fusion cytokine CAMPILs co-expression biological preparation. The antimicrobial peptide fusion cytokine CAMPILs provided by the present invention is as follows A1), A2) or A3): A1) the amino acid sequence is the protein of sequence 1 in the sequence listing; A2) the amino acid sequence shown in sequence 1 in the sequence listing is passed through a or several amino acid residue substitutions and / or deletions and / or additions and proteins with the same function; A3) A fusion protein obtained by linking tags at the N-terminal or / and C-terminal of A1) or A2). Experiments have proved that CAMPILs of the present invention can promote the proliferation of lymphocytes, red blood cells and white blood cells, inhibit the growth of pathogenic microorganisms, promote the secretion of non-specific antibodies (IgG, IgG1, IgG2a) and disease-specific antibodies, and improve the immunity and survival of animals. Rate.

The invention relates to bursa of Fabricius heptapeptide with immune regulation effect and application thereof in immunity (in improving the immune capability of animals, improving the immune effect of vaccines and affecting the activity of tumor cells), and belongs to the field of immunology. The molecular weight of the separated heptapeptide is 722.240, the amino acid sequence is EPASGMM, and the heptapeptide has a simple structure, no toxic or side effect and extremely weak immunogenic property. The heptapeptide can be separated and extracted from bursa of Fabricius, also can be chemically synthesized, has low cost, and can be massively produced. The bursa of Fabricius heptapeptide has induction effect on the production of antibody and subtype thereof, and meanwhile can regulate the production of cell factors, partition of T lymphocytes and proliferation of spleen cells, and promote the immune reaction of the cells. The bursa of Fabricius heptapeptide is an immune regulation factoron functions, has wide application prospect on the aspects of immune regulation, immune therapy and the like, and can be applied in the fields of basic immune research, clinical application research and the like.

The invention discloses engineered yeast of a fish natural killer cell enhancer and an application thereof. The puffer fish NKEF engineered yeast is named as saccharomyces cerevisiae INVSC1-pYES2-NKEFwith the preservation number of CGMCC1847. The engineered yeast is obtained by inserting the puffer fish NKEF gene into the place between EcoRI and XhoI restriction enzyme cutting sites of a pYES2 carrier to construct a recombinant plasmid and then introducing the recombinant plasmid into the expression host saccharomyces cerevisiae. The engineered yeast is cultivated under the condition of 30 oC, induced by 2% galactose, and the expression of NKEF recombinant protein can reach more than 20%. The anti-disease application of the above-mentioned engineered yeast in aquatic animal breeding is also disclosed, and the effect of improving the anti-infective capability and autoimmune capability of aquaculture animals can be achieved.