Microorganism feed additive capable of promoting goose liver development and preparation method thereof
A microbial feed and additive technology, which is applied in animal feed, animal feed, additional food elements, etc., can solve the problems of foie gras difficulty in reassurance, slow decomposition of plant fiber, etc., to reduce fecal phosphorus discharge, reduce material-to-weight ratio, and reduce pollution effect
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Embodiment 1
[0036] Step 1: Strain screening and seed production
[0037] a. Strain screening: take chyme from the small intestine of healthy goose as the bacterial source, and use yeast extract 7.5g, peptone 7.5g, glucose 10g, KH 2 PO 4 2g (potassium dihydrogen phosphate), tomato juice 100ml, Tween (Tween) 80 0.5ml, distilled water 900ml pH 7.0 lactic acid bacteria medium for lactic acid bacteria isolation, screening Lactobacillus plantarum, Bifidobacterium, Lactobacillus casei and Bacillus subtilis; Separation of brewer's yeast in the beer fermentation broth with the Mai's agar medium composed of 1g of glucose, 1.8g of potassium chloride, 2.5g of yeast extract, 8.2g of sodium acetate, 15-20g of agar, and 1000ml of distilled water. Bacterial strain is preserved, and the present invention uses following bacterial classification: Lactobacillus plantarum (ATCC8014) Lactobacillus plantarum, Bifidobacterium Lactobacillus casei (ATCC7469), Lactobacillus casei (ATCC7469) Lactobacillus casei, Sa...
Embodiment 2
[0051] The first step: strain screening and production seed production are the same as in Example 1;
[0052] The second step: the preparation of compound bacterial agent
[0053] a. Take by weighing each production strain in the eggplant bottle according to the following weight ratio: 30% of Lactobacillus plantarum, 35% of Bifidobacterium, 15% of Lactobacillus casei, 20% of Saccharomyces cerevisiae, 10% of Bacillus subtilis;
[0054] b. Mix the production strains and add them in a proportion of 6% to the composite bacteria composed of 0.5% molasses, 0.3% peptone, 1% glucose, 0.2% sodium chloride, 4% yeast extract, 0.8% magnesium sulfate, and 93.2% water In the culture medium, adjust the pH value to 7, put it into the container and heat it to 121°C for sterilization for 40 minutes, and ferment it in the fermenter for 6 days after cooling to 30°C;
[0055] The third step: the expansion and domestication of compound bacterial agents
[0056] a. Mix the composite bacterial agen...
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