Streptococcus thermophilus and application thereof

A technology of Streptococcus thermophilus and strains, applied in the field of microbiology, can solve the problems of imperfect preparation of Streptococcus thermophilus starter, poor strain viability, low exopolysaccharide production, etc., to achieve large-scale industrial application and improve quality Structural characteristics, the effect of simple process

Inactive Publication Date: 2016-08-24
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of incomplete preparation of Streptococcus thermophilus starter, poor strain vigor and low exopolysaccharide yield in the prior art, and provides a Streptococcus thermophilus that can solve the above problems and its application

Method used

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  • Streptococcus thermophilus and application thereof
  • Streptococcus thermophilus and application thereof
  • Streptococcus thermophilus and application thereof

Examples

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preparation example Construction

[0023] The preparation method of the culture medium used in the embodiment is as follows:

[0024] Skim milk culture solution: skim milk powder 120g / L, sterilized at 115°C for 15min.

[0025] Skim milk medium: skim milk powder 120g / L, bacterial agar powder 20g / L, sterilized at 115°C for 15min.

Embodiment 1

[0026] Example 1. Screening and Identification of Streptococcus thermophilus AR-333 Strain

[0027] 1. Sample dilution preparation and strain isolation

[0028] Add 1 g of naturally fermented dairy products to 15 mL of sterile water, and shake vigorously for 10 min. Use concentration gradient dilution method to dilute, take 100uL of the diluted solution and spread it on the skim milk agar medium plate, culture it anaerobically at 35~42℃ for 40~56h, select the strain with large colony, fast growth and high viscosity of filament, and further draw Line separation and purification. In this embodiment, the preferred culture temperature is 42° C., and the preferred anaerobic culture time is 48 h.

[0029] 2. Primary screening of strains

[0030] Inoculate the isolated strain with high thread-picking viscosity in 1.1 into the skim milk liquid medium, and culture it statically at 35-42°C for 8-12 hours to prepare a seed liquid; then inoculate the seed liquid with an inoculum volume...

Embodiment 2

[0049] Embodiment 2. Using Streptococcus thermophilus AR-333 strain as starter to prepare fermented milk and cheese

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Abstract

The invention provides a streptococcus thermophilus AR-333 strain and application thereof. The streptococcus thermophilus AR-333 strain is obtained through being separated from naturally fermented milk, and the collection number of the strain is CGMCC 10262. The streptococcus thermophilus AR-333 is fermented for 16h under the condition of 40 DEG C by skimmed milk; the yield of extracellular mucopolysaccharide reaches 256.97 mg/L; meanwhile, the streptococcus thermophilus is used as a yoghourt fermenting agent; the viscosity of yoghourt can be obviously improved; the tissue state of the yoghourt can be improved; good flavor is realized; the generated extracellular mucopolysaccharide has an immunologic competence regulating function; macrophages RAW264.7 can be simulated to release NO.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of streptococcus thermophilus and its application in the preparation of fermented milk or cheese and the extraction of extracellular polysaccharides. Background technique [0002] Exopolysaccharides (EPS) are water-soluble polysaccharides secreted outside the cell wall and into the environment by some special microorganisms during their growth and metabolism. This microbial polysaccharide has a short production cycle and is not limited by seasons, regions, and pest conditions. Compared with plant polysaccharides, it has strong market competitiveness and broad development prospects. [0003] Streptococcus thermophilus belongs to this group of microorganisms. Streptococcus thermophilus belongs to lactic acid bacteria (LAB), which is a food-grade industrial production bacterium. The EPS secreted by it is recognized as green and safe in the dairy industry, and can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P19/04A23C9/12A23C19/032C12R1/46C12R1/66
CPCA23C9/1203A23C19/032C12N1/02C12N1/20C12P19/04C12N1/205C12R2001/46
Inventor 艾连中任伟夏永军王光强印伯星张汇熊志强
Owner UNIV OF SHANGHAI FOR SCI & TECH
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