50 results about "Sample dilution" patented technology

The invention discloses a preparation method of a CGRP detection kit and application of the CGRP detection kit in migraine diagnosis, and belongs to the technical field of biological medicine detection. According to the invention, alpha-CGRP polypeptide coupled KLH protein is subjected to efficient animal immunization, an antibody with good affinity is screened by using methods such as enzyme-linked immunosorbent assay, cross pairing analysis is carried out, and final antibody sequence information is obtained through sequencing. The paired monoclonal antibody screened by the invention has high sensitivity (KD value of 1nM), the limit of detection (LoD) can be as low as pg / mL level, a low-concentration CGRP sample can be effectively detected, the matrix effect of human serum / plasma can be effectively neutralized by an optimized sample diluent formula, and the repeatability is high (in-plate and inter-plate CV values of 1t and 10%). According to the invention, the kit prepared from the specific paired antibody is systematically and closely linked with the indication function of migraine for the first time, and a clinical diagnosis direction is provided.

An analyzer having an inner chassis surrounded by a housing includes: sample and diluent probes, a mixing housing including a first mixing chamber and a second mixing chamber, a flow cytometer including a flow unit, and sample and sheath pumps configured to perform a first set of tasks and a second set of tasks, respectively. The first set of tasks includes: aspirating a sample into the sample probe; dispensing a sample from the sample probe into the first and second mixing chambers; delivering a first sample diluent mixture to the flow unit; and delivering a second sample diluent mixture to the flow unit. The second set of tasks includes: dispensing a sheath into the flow unit in a manner cooperating with delivering the first sample diluent mixture to the flow unit; and dispensing a sheath into the flow unit in a manner cooperating with delivering the second sample diluent mixture to the flow unit.

This invention provides a kit for detecting anti-peroxidase-1-IgG antibodies, comprising the antigen protein peroxiredoxin-1 (peroxidase-1), a solid-phase carrier, labeled antibody, antigen dilution buffer, sample dilution buffer, antibody dilution buffer, substrate chromogenic agent, washing buffer, standards, positive control, and negative control. This kit utilizes an indirect reaction principle combined with magnetic microparticle chemiluminescence immunoassay to detect anti-peroxidase-1-IgG antibodies in the serum of the test sample. This invention is the first to identify autoantibodies against the target antigen peroxidase-1 in the serum of patients with autoimmune nephrotic syndrome. The provided kit provides a basis for domestic and international research on the molecular mechanisms and clinical diagnosis and treatment of autoimmune nephrotic syndrome related to peroxidase-1 and peroxidase-1-IgG autoantibodies.

This disclosure relates to a sample diluent and its preparation method. Specifically, this disclosure relates to a sample diluent composed of Tris, Tween-20, BSA, mixed serum, and PC300. A key improvement is that the mixed serum is prepared by mixing goat serum and fetal bovine serum at a volume ratio of 2:1, and the mixed serum accounts for 40% of the sample diluent. The sample diluent of this disclosure is designed for the detection of lambda and kappa type free light chains, significantly reducing sample matrix interference, improving detection specificity and stability, and solving the technical problem of disproportionate dilution after high-value sample dilution. It is suitable for clinical testing, biomedical research, and other scenarios.

The invention relates to the technical field of instrument measuring range expansion, in particular to an instrument measuring range intelligent expansion optimization method based on sample dilution, which comprises the following steps: acquiring an effective measuring range threshold value through an intelligent sensor, counting relative standard deviation and signal-to-noise ratio of a concentration interval in the measuring range, and acquiring a precision distribution curve; performing original sample test on a to-be-tested sample, directly detecting if a response value is within a range, starting an intelligent prediction model according to pollutant types and matched dilution parameters of the to-be-tested sample if the response value exceeds the range, and predicting a recommended dilution multiple according to initial test signal intensity and the sample; when the prediction confidence is lower than a threshold value, triggering gradient scanning; carrying out parallel sample measurement on the diluent to calculate relative standard deviation, executing two adjacent dilution multiples if the distance difference between the diluent and the segmented boundary is smaller than a threshold value, and carrying out mutual verification through concentration consistency of an overlapping region; and performing full-process detection on different dilution multiples, establishing a dilution correction model, updating a correction coefficient of the dilution multiples, and updating a range boundary and a precision distribution curve.

The invention relates to a specific protein analyzer sample track sample injection step cycle dilution reexamination method. The method comprises the following steps: 1, sucking a sample to be detected, and respectively adding the sample to be detected into a reaction cup and a dilution cup; 3, judging whether the analyzer outputs an effective detection result or not, if yes, executing the step 6, and if not, executing the step 4; 4, the dilution puncture needle sucks the sample diluent and the to-be-detected sample in the dilution cup and distributes the sample diluent and the to-be-detected sample in the dilution cup to the dilution cup in an idle state for dilution, and the diluted to-be-detected sample is obtained; 5, sucking the diluted to-be-detected sample into the idle reaction cup by the sample adding needle, and executing the step 2; 6, emptying and cleaning the reaction cup and the dilution cup corresponding to the to-be-detected sample, according to the scheme, the dilution and re-detection process of the sample does not need to be manually intervened, when the detection result is not output, the sample can be automatically diluted and re-detected through the dilution puncture needle and the sample adding needle, the re-detection time is greatly shortened, and the detection efficiency is improved.

The invention provides a system for testing the content of iron with different valence states in mineral aggregate transfer leaching liquid. The system comprises a sample dilution unit, an auxiliary agent unit and a sample analysis unit, the sample diluting unit is used for diluting a sample to be detected to obtain a diluted sample; the auxiliary unit is used for providing reaction liquid and an indicator; the feeding end of the sample analysis unit is respectively connected with the discharging end of the sample dilution unit and the discharging end of the auxiliary agent unit, and is used for enabling a diluted sample from the sample dilution unit and a reaction liquid and an indicator from the auxiliary agent unit to be subjected to valence state conversion reaction and chromogenic reaction of corresponding valence state iron in sequence; and carrying out ultraviolet irradiation and analysis on the obtained chromogenic reaction mixed solution to obtain the content value of the iron in the corresponding valence state. According to the method and the system, the content of iron with different valence states in the transfer leaching liquid in the wet metallurgy mineral aggregate transfer leaching process can be simply, conveniently and accurately tested on line in real time.

A pouch for a diagnosis item includes (i) a pocket portion with a storage space configured to hold a buffer that has an elongate shape and is configured to hold a sample diluent, and (ii) a buffer supporter formation portion that is connected to one side of the pocket portion. The buffer supporter formation portion includes a buffer support hole that is sized to receive the buffer and support the buffer such that the buffer is oriented in an upright state to avoid spilling of the sample diluent. The buffer supporter formation portion includes a shelf supported by leg portions. The shelf includes the buffer support hole. The buffer supporter formation portion is configured to allow bending of the buffer supporter formation portion into a U shape, and to hold the U shape after being detached from the pocket portion.

A system and method to extend a dynamic range of fluorescence measurements. The system includes a neutral density filter stack apparatus including a plurality of neutral density filters. The neutral density filter stack modulates the intensity of light from a source. This modulation improves the ability to detect emissions from a wider-range of fluorophore concentrations, specifically addressing inner-filter effects at higher concentrations that would normally require sample dilution. It can be used in a system in which absorbance and fluorescence measurements are carried out, wherein the intensity of the light source is modulated with the apparatus for the fluorescence measurements. In this hybrid system, the stack modulates the intensity of the excitation light source for fluorescence measurements to be made at very low concentrations of analyte. The hybrid system also includes optics for absorbance measurements to be made at concentrations of analyte that are higher than those detected by fluorescence.

The application provides an incubation device for immunohistochemistry, a staining treatment all-in-one machine and a control method thereof. The device comprises a cabin body, a carrier and a condensation flow guide plate. The carrier is provided with a humidification flow channel, which is divided into multiple liquid accumulation pools by a liquid distribution baffle, and the overflow principle is used to ensure uniform humidity and inhibit liquid accumulation during movement. The condensation flow guide plate is provided with a heating surface and a condensation surface, the temperature of the condensation surface is controlled to be lower than the dew point, vapor is induced to condense on the condensation surface and is discharged to a waste liquid outlet through the flow guide surface, and condensate droplets are prevented from falling on the slide. A driving assembly drives the carrier to move between an incubation position and an operation position and drives the cabin door to move. The application combines zoned temperature control, induced condensation and physical directional flow guidance, solves the risk of sample dilution caused by random condensation of condensate in a high humidity environment, simplifies the overall structure of the machine and improves the automation integration degree.

This application discloses a sample dilution method, a detection method, a detection device, and a storage medium. The sample dilution method includes: estimating at least one dilution ratio; determining another dilution ratio based on the target dilution ratio of the sample and the at least one dilution ratio; adjusting the at least one dilution ratio until the other dilution ratio meets a preset requirement, and determining that the current at least one dilution ratio and the other dilution ratio are respectively the dilution ratios used in one dilution process within a series of dilutions of the sample. Through the above method, this application can improve the accuracy of the dilution ratio of the target solution when performing multiple dilutions.

The invention belongs to the technical field of detection, and particularly discloses a measurement uncertainty evaluation method for measuring immune factors in serum by an ELISA (enzyme-linked immuno sorbent assay) method. According to the method, ELISA detection is carried out on immune factors (such as IL-1beta) in serum of an experimental animal in contact with a medical instrument according to standards such as GB / T16886.20-2015, system analysis comprises nine types of uncertainty sources such as repeated detection, standard substance preparation and sample dilution, the standard uncertainty of each component is calculated through mathematical methods such as rectangular distribution and a least square method, and the standard uncertainty of each component is calculated according to the standard uncertainty. And synthesizing the standard uncertainty and calculating the expansion uncertainty, and finally outputting a standard detection result. The method comprehensively covers the uncertainty source, is rigorous in computational logic and clear in key quality control factors, provides a scientific and complete uncertainty evaluation scheme for medical instrument immunogenicity evaluation and immune factor determination in other experimental scenes, is beneficial to laboratory quality control and detection result optimization, and has wide practicability.

The utility model belongs to the technical field of auxiliary equipment for food microbiological detection, and discloses a homogenizing bag frame, the homogenizing bag frame is used for placing a homogenizing bag, the homogenizing bag is provided with a positioning hole, the homogenizing bag frame comprises a first connecting piece, support frames and hooks, the first connecting piece and two adjacent support frames form a support frame, and the first connecting piece and the two adjacent support frames form the support frame. A worker aligns the positioning holes with the hooks and puts one homogenizing bag into the corresponding supporting frame, and the other end of each hook is inserted into the corresponding positioning hole to hook the homogenizing bag, so that the homogenizing bags are fixed, the homogenizing bags can be fixed on the hooks in the process of inclining the whole homogenizing bag frame to suck sample diluent in the homogenizing bags, and the sample diluent in the homogenizing bags can be sucked by the hooks. The sample diluent can be sucked more conveniently, and the situation that the sample diluent in the homogenizing bag is spilled out and the loss of the sample diluent is caused due to the fact that the homogenizing bag slides out of the upper portion of the supporting frame is avoided.

The invention discloses application of serum alpha-non-red blood cell ghost protein 1 in preparation of drugs for diagnosing heart light chain amyloidosis diseases, and belongs to the technical field of biological medicines. The serum SPTAN1 protein is used for preparing the medicine for diagnosing the heart light-chain amyloidosis disease, the medicine is limited to be an enzyme linked immunosorbent assay kit, and the kit comprises an antibody of the SPTAN1 protein and further comprises a pre-coated plate, a standard substance, a coating buffer solution, a sample diluent, a confining liquid, horse radish peroxidase, an eluent and a color developing agent. The concentration of the SPTAN1 protein in the serum is measured through an enzyme linked immunosorbent assay kit, and early diagnosis of the heart AL amyloidosis disease is carried out. The invention provides a novel, efficient and sensitive biomarker, namely the serum SPTAN1 protein, early diagnosis of heart AL amyloidosis is realized by detecting the concentration of the SPTAN1 protein in serum, and the serum SPTAN1 protein has an important clinical application prospect.

The invention relates to a pancreatic ductal adenocarcinoma exosome marker detection kit based on a chemiluminescence method as well as a preparation method and application of the pancreatic ductal adenocarcinoma exosome marker detection kit. The kit comprises an exosome targeted capture processing component, an REG1A detection component, an REG1B detection component and a common component, the exosome targeted capture treatment component consists of EphA2 immunocapture magnetic beads, a high-efficiency capture buffer solution and an exosome lysis solution; the REG1A detection component is composed of REG1A capture magnetic particles, a horse radish peroxidase labeled anti-REG1A detection antibody, a REG1A calibration product and a REG1A quality control product; the REG1B detection component is composed of REG1B capture magnetic particles, a horse radish peroxidase labeled anti-REG1B detection antibody, a REG1B calibration product and a REG1B quality control product; the kit is composed of a 20 * concentrated washing solution, a sample diluent and a chemiluminescent substrate solution. The detection kit can effectively eliminate interference of free protein and other exosomes, the detection specificity reaches 98.3%, the sensitivity reaches 1.6-1.9 pg / mL, and a more reliable auxiliary detection basis is provided for clinical decision making.

The utility model relates to a detection sample dilution system, which comprises a reaction cup, a first pump body and a sampling needle, the reaction cup mixes a sample and a diluent, and fully shakes up to obtain a first mixed solution; the reaction cup is also used for discharging the residual first mixed solution; mixing the first mixed solution and the diluent in the reaction cup, and fully shaking up to obtain a second mixed solution; the first pump body is communicated with the reaction cup; the first pump body pumps the diluent into the reaction cup; the first pump body pumps a certain amount of first mixed liquid in the reaction cup for temporary storage; after the residual first mixed solution is discharged from the reaction cup, a certain amount of the first mixed solution is introduced into the reaction cup again; the first pump body injects a diluent into the reaction cup; the sampling needle extracts a sample and injects the sample into the reaction cup; the sampling needle injects the reagent into the reaction cup. Compared with the prior art, the rapid dilution device can rapidly dilute a sample solution, the dilution multiple is flexible and adjustable, the sample testing efficiency is improved, and the sample dilution cost can be reduced.

Sample preparation systems and methods are described having pump control, valve configurations, and control logic that facilitate automatic, inline preparation dilutions of a sample according to at least two dilution operating modes. A system embodiment includes, but is not limited to a first pump configured to drive a carrier fluid; a second pump configured to drive a diluent; and a plurality of selection valves fluidically coupled with the first pump and the second pump, the plurality of selection valves being configured to direct fluid flows from the first pump and the second pump according to at least two modes of operation to provide a single-stage sample dilution according to a first operating mode and to provide a dual-stage sample dilution according to a second operating mode.

The invention provides a sample delivery system for an analytical instrument, comprising: a valve assembly comprising one or more valves, the valve assembly configured to receive an external sample from a sample source; an external sample reservoir coupled via the valve assembly to a sample dilution junction, wherein the valve assembly is configurable to alternatively permit either (i) flow of the external sample from the sample source to the external sample reservoir or (ii) flow of the external sample from the external sample reservoir to the sample dilution junction; a first fluid pump to control a flow of the external sample from the external sample reservoir to the sample dilution junction; a second fluid pump to control a flow of diluent to the sample dilution junction, thereby diluting the external sample to produce a diluted sample; and an analysis sample reservoir configured to deliver an analysis sample contained therein to an analysis device of the analytical instrument, wherein the analysis sample reservoir is coupled via the valve assembly to the sample dilution junction, and wherein the valve assembly is configurable to alternatively permit either (i) flow of the external sample along a continuous flow path from the sample source to the analysis sample reservoir, thereby by-passing the external sample reservoir, or (ii) flow of the diluted sample from the sample dilution junction to the analysis sample reservoir.

The utility model provides a sample dilution device for liquor flavor substance extraction belongs to liquor dilution technical field, including dilution cylinder, be located dilution cylinder bottom's straight flow pipe, and be located dilution cylinder top's cylinder cover, be located the alcohol concentration detector, main sample outlet pipe and stop valve on straight flow pipe outer lateral wall, main sample outlet pipe is located between alcohol concentration detector and stop valve, and main sample outlet pipe is located alcohol concentration detector below, be located the support frame of dilution cylinder inner lateral wall close bottom end position, and place in the positive screw spiral blade no.

Laboratory Apparatus for Sample Dilution with Sliding Valve Geometry.

Systems and methods for controlling sample dilution in an analytical instrument system comprise transmitting an injection command to an autosampler to inject a predetermined volume of air into a sample tubing coupled to an analytical instrument; and transmitting, subsequent to the injection of the predetermined volume of air, a fill command to the autosampler to aspirate a volume of sample into the sample tubing, wherein the predetermined volume of air is configured to form an immiscible barrier between the volume of sample and a solvent within the sample tubing.

The invention relates to a sample dilution method and a sample analysis instrument. The method comprises the following steps: obtaining dilution configuration information, wherein the dilution configuration information comprises dilution parameters and pre-distributed dilution digits corresponding to the dilution parameters; when the sample analysis instrument is in a standby state, target dilution positions on the sample analysis instrument are determined according to the pre-distributed dilution digits, and corresponding diluents are distributed to the target dilution positions in sequence according to the dilution parameters; and in response to a submission request of the target test task, sequentially adding the to-be-diluted samples to the target dilution positions pre-distributed with the diluent. According to the scheme provided by the invention, the use cycle of the sample can be effectively shortened, namely, the cycle of removing all the sample from the sample analysis instrument.

The invention relates to the technical field of sample analysis, and discloses a full-automatic specific protein analyzer intelligent operation analysis management platform which comprises a full-automatic specific protein analyzer and an operation management system used for controlling and managing the full-automatic specific protein analyzer. The puncture automatic dilution module is used for self-defining a sample dilution multiple and automatically diluting a to-be-detected sample in a stepped circulation manner; the puncture automatic dilution module comprises a puncture starting sub-module, an automatic dilution starting sub-module, an automatic dilution setting sub-module and an automatic dilution application sub-module, the puncture starting sub-module is used for starting a dilution puncture needle to suck a sample and a diluent at a specified position, and the automatic dilution starting sub-module is used for selecting a dilution mode; the automatic dilution setting sub-module is used for setting an automatic dilution multiple, and the automatic dilution application sub-module is used for sequentially diluting the samples to be detected according to the selected dilution mode. The sample dilution efficiency can be improved, the inaccurate dilution ratio is avoided, and the accuracy of a detection result is improved.

The utility model discloses a sample on-line dilution and separation device, which comprises a sample mixing mechanism, a sample filling mechanism and a waste liquid separation mechanism, and the sample mixing mechanism comprises a mounting structure, a linear power source arranged on the mounting structure, a mixing power source driven by the linear power source to linearly move back and forth, and an eccentric mixing assembly. The sample mixing mechanism disclosed by the utility model is provided with at least two eccentric mixing assemblies, and the eccentric mixing assemblies can linearly move back and forth, so that the linear back and forth movement of the eccentric mixing assemblies can be realized; the at least two eccentric uniform mixing assemblies can alternately complete cup receiving, liquid injection, uniform mixing and the like, so that flexible dilution (single time, two times or multiple times) of each sample is realized, the detection requirements of high-concentration samples are met, continuous dilution of a plurality of samples is realized, the sample dilution efficiency is improved, and the detection efficiency is further ensured; residual liquid is pumped away by the waste liquid separation mechanism, so that empty reaction cups are discarded, and biological hazards caused by residual samples are reduced.

The application belongs to the technical field of instant diagnosis, and particularly relates to a sample sampling and detecting device. The sample sampling and detecting device comprises a sample dilution component and a sampling component. The sample dilution component comprises a sample dilution box, and the sample dilution box has a containing cavity for storing sample diluent. The sampling component comprises a sampling head, a detection test paper and a flow guide structure. The sampling head has a liquid inlet, and the sampling head can be inserted into the containing cavity so that the sample diluent enters the interior of the sampling head through the liquid inlet and mixes with the sample. The flow guide structure is arranged in the interior of the sampling head, and the flow guide structure is provided with a flow guide part. A lateral flow channel exists between the flow guide part and the inner wall of the sampling head. By using the sample sampling and detecting device, the sample diluent can be prevented from overflowing from the gap between the outer wall of the sampling head and the inner wall of the sample dilution box during the process of inserting the sampling head into the sample dilution box. Meanwhile, the waste of the sample diluent caused by splashing can be prevented, and the full contact between the sample diluent and the detection test paper can be ensured.

This invention discloses a DNA detection method based on LAMP and immunochromatography, belonging to the field of biomedical detection technology, including the following steps: S1, LAMP amplification and product labeling: adding sample dilution buffer to a LAMP reaction tube, and using FITC-labeled specific primers to perform LAMP isothermal amplification of the target DNA in the sample; S2, immunochromatographic reagent detection: using a double-antibody sandwich immunochromatography method, the FITC-labeled LAMP product is visualized and detected by colloidal gold; S3, result determination. This DNA detection method based on LAMP and immunochromatography can achieve efficient amplification of target DNA under isothermal conditions using LAMP primers, and label the amplified product by pre-incorporating FITC-labeled specific primers. Subsequently, the signal is visualized and read out using a double-antibody sandwich immunochromatography method with colloidal gold, thereby achieving highly sensitive, highly specific, and rapid visual detection of target DNA.

The application belongs to the field of metal detection of phosphine, and provides a metal detection method of phosphine, which uses an ICP-MS analysis instrument, injects a sample diluted by a proper multiple into an inductively coupled plasma, ionizes metal elements, separates ions according to mass-to-charge ratios by using a mass spectrometer, and uses a corresponding ion detector to perform signal detection and counting to obtain a response value of an element to be detected of an unspiked sample. An internal standard element with a proper concentration is selected and added to the sample. A standard solution of the element to be detected with a proper concentration gradient is selected, added to the sample, and detected to obtain several standard points. An internal standard correction standard curve is drawn with the ratio of the response value of the element to be detected to the response value of the internal standard element as the y-axis and the ratio of the concentration of the element to be detected to the concentration of the internal standard element as the x-axis. The application solves the problem of sample pretreatment of phosphine, and has high accuracy and precision after detection by using an ICP-MS optimized instrument, and can provide reliable analysis results.

The utility model provides a kind of full-automatic flow cytometry, including sample entering module, reagent disc module, reagent needle module, sample sample adding module, dilution disc module, gripper arm group module, incubation disc module, magnetic separation module, consumable disc module, sample loading discarding module, flow detection module, each module cooperates to realize the whole process of sample dilution, sampling distribution, reagent distribution, constant temperature incubation, reaction system cleaning, flow detection and other flow experiments.The full-automatic flow cytometry can automatically complete the pretreatment and detection of flow sample, improve the consistency and reliability of sample pretreatment, reduce detection difference, simultaneously compatible protein marker and cell detection, support protein detection project and cell analysis project without difference on-machine operation, instrument can automatically identify detection project, correspondingly grab different detection tube, execute different program, without artificial distinction, improve detection efficiency.

The utility model discloses an industrial slurry sampling dilution device, specifically relates to sampling dilution technical field, including the cabinet, be provided with dilution subassembly on the cabinet, dilution subassembly includes the lining frame of setting in the cabinet, the inside of lining frame is provided with the material taking jar for material storage. The utility model discloses through the device through the filter setting, can effectively intercept the solid particle or agglomerate in high concentration slurry, avoid such impurities to enter the subsequent dilution and analysis link. Meanwhile, in combination with the backflow design of pump machine and connecting valve, the pump machine output end can be through connecting valve and return to the filter secondary filtration with slurry, further improve the filtering effect, ensure that there is no interfering impurity in the slurry after dilution, provide the impurity -free sample for the subsequent microscope morphological observation, filtration, centrifugal separation etc. Analysis operation, solve the problem that the analysis result deviation of impurity in the prior art.

The invention provides a purification method of human C-reactive protein, and particularly relates to the technical field of biological medicine. Obtaining a sample containing the C-reactive protein, wherein the sample is a CHO cell fermentation broth or a supernatant obtained by centrifuging the CHO cell fermentation broth; diluting the sample: diluting the sample by using 20-100 mM of MTris, 0-50 mM of NaCl and a diluent with the pH value of 8.0, reducing the conductivity of the sample to 0-4.5 ms / cm, and balancing the pH value of the sample to 7.8-8.2 to obtain a protein sample. Crude purification: carrying out crude purification on a protein sample obtained through a strong anion column to obtain a crude pure sample; diluting the crude pure sample: diluting the crude pure sample by using a diluent with the pH value of 8.0, namely 20-100 mM of MTris, 0-50 mM of NaCl and 1-5 mM of MEDTA, reducing the conductivity of the crude pure sample to 0-4.5 ms / cm, and balancing the pH value of the crude pure sample to 7.8-8.2; performing fine purification: performing fine purification on the diluted coarse pure sample through a weak anion column to obtain a target product; the method is simple in process and has the advantages of low production cost and high purity and yield.