99 results about "Sample dilution" patented technology

The invention discloses a preparation method of a CGRP detection kit and application of the CGRP detection kit in migraine diagnosis, and belongs to the technical field of biological medicine detection. According to the invention, alpha-CGRP polypeptide coupled KLH protein is subjected to efficient animal immunization, an antibody with good affinity is screened by using methods such as enzyme-linked immunosorbent assay, cross pairing analysis is carried out, and final antibody sequence information is obtained through sequencing. The paired monoclonal antibody screened by the invention has high sensitivity (KD value of 1nM), the limit of detection (LoD) can be as low as pg / mL level, a low-concentration CGRP sample can be effectively detected, the matrix effect of human serum / plasma can be effectively neutralized by an optimized sample diluent formula, and the repeatability is high (in-plate and inter-plate CV values of 1t and 10%). According to the invention, the kit prepared from the specific paired antibody is systematically and closely linked with the indication function of migraine for the first time, and a clinical diagnosis direction is provided.

Disclosed herein are improved methods for quantifying monoclonal antibody (mAb) titer using Protein A affinity High-Performance Liquid Chromatography (HPLC). The methods address inaccuracies caused by mAb adsorption to sample vial surfaces by incorporating a surfactant into the mobile phase used for sample dilution. This approach enhances the accuracy, linearity, and robustness of the titer assay, making it suitable for process control and quality assessment in biopharmaceutical development and manufacturing. The methods can be applied as a platform approach for various mAb products.

An analyzer having an inner chassis surrounded by a housing includes: sample and diluent probes, a mixing housing including a first mixing chamber and a second mixing chamber, a flow cytometer including a flow unit, and sample and sheath pumps configured to perform a first set of tasks and a second set of tasks, respectively. The first set of tasks includes: aspirating a sample into the sample probe; dispensing a sample from the sample probe into the first and second mixing chambers; delivering a first sample diluent mixture to the flow unit; and delivering a second sample diluent mixture to the flow unit. The second set of tasks includes: dispensing a sheath into the flow unit in a manner cooperating with delivering the first sample diluent mixture to the flow unit; and dispensing a sheath into the flow unit in a manner cooperating with delivering the second sample diluent mixture to the flow unit.

The invention belongs to the technical field of instant diagnosis, and particularly relates to a sample sampling and detecting device. The sample sampling and detecting device comprises a sample diluting assembly and a sampling assembly, the sample dilution assembly comprises a sample dilution box, and the sample dilution box is provided with a containing cavity for storing sample dilution liquid; the sampling assembly comprises a sampling head, detection test paper and a flow guide structure, the sampling head is provided with a liquid inlet, the sampling head can be inserted into the accommodating cavity so that a sample diluent enters the sampling head through the liquid inlet and is mixed with a sample, and the flow guide structure is arranged in the sampling head and is provided with a flow guide part; a lateral flow channel is formed between the flow guide part and the inner wall of the sampling head. By using the sample sampling and detecting device in the technical scheme, a sample diluent can be prevented from overflowing from a gap between the outer wall of the sampling head and the inner wall of the sample dilution box in the process that the sampling head is inserted into the sample dilution box, meanwhile, waste caused by splashing of the sample diluent can be prevented, and full contact of the sample diluent and detection test paper is ensured.

The present invention discloses a rapid hydrogen peroxide test kit and a method of using the same, the rapid hydrogen peroxide test kit comprising a sample diluent and a test strip containing a chromogen for detection and a chromogen for quality control in a dry environment. The sample diluent comprises 100-500 mM ammonium oxalate, 0.3-2.0 mM 8-hydroxyquinoline, 50-500 mM sodium chloride, and 10-100 mM of a first pH adjuster for pH 4.5-7.0.

This invention provides a kit for detecting anti-peroxidase-1-IgG antibodies, comprising the antigen protein peroxiredoxin-1 (peroxidase-1), a solid-phase carrier, labeled antibody, antigen dilution buffer, sample dilution buffer, antibody dilution buffer, substrate chromogenic agent, washing buffer, standards, positive control, and negative control. This kit utilizes an indirect reaction principle combined with magnetic microparticle chemiluminescence immunoassay to detect anti-peroxidase-1-IgG antibodies in the serum of the test sample. This invention is the first to identify autoantibodies against the target antigen peroxidase-1 in the serum of patients with autoimmune nephrotic syndrome. The provided kit provides a basis for domestic and international research on the molecular mechanisms and clinical diagnosis and treatment of autoimmune nephrotic syndrome related to peroxidase-1 and peroxidase-1-IgG autoantibodies.

The present invention provides a kit for detecting antigen myosin 1 IgG antibodies, which is composed of antigen protein Tropomyosin 1 (tropomyosin 1), a solid phase carrier, a standard, a positive quality control product, a negative quality control product, a labeled antibody, a substrate color developing solution, an antibody diluent, an antigen diluent, a sample dilution buffer, a washing solution, a stop solution, etc. The present invention utilizes the target antigen Tropomyosin 1 corresponding to the anti-Tropomyosin 1 IgG antibody, and uses human anti-label peptide IgG as a standard for quantitative detection to perform anti-Tropomyosin 1 IgG antibody detection on the antigen-antibody complex formed in the serum. The kit of the present invention is easy to operate, has very good reagent stability, is easy to store, and fills the gap in identifying biomarkers for patients with immune nephrotic syndrome at home and abroad.

The invention provides a kit for detecting the concentration of Venoclarias and a Venoclarias concentration detection method, and belongs to the technical field of in-vitro diagnos.The kit comprises a calibrator, an internal standard mother solution, a quality control product, an internal standard-sample releasing agent, a sample dilution treating fluid and a mobile phase. And the detection of low-concentration Venokacrah can be realized. The kit provided by the invention also has the advantages of high sensitivity, strong anti-interference capability and high accuracy.

This disclosure relates to a sample diluent and its preparation method. Specifically, this disclosure relates to a sample diluent composed of Tris, Tween-20, BSA, mixed serum, and PC300. A key improvement is that the mixed serum is prepared by mixing goat serum and fetal bovine serum at a volume ratio of 2:1, and the mixed serum accounts for 40% of the sample diluent. The sample diluent of this disclosure is designed for the detection of lambda and kappa type free light chains, significantly reducing sample matrix interference, improving detection specificity and stability, and solving the technical problem of disproportionate dilution after high-value sample dilution. It is suitable for clinical testing, biomedical research, and other scenarios.

The invention provides a sample ion detection method and a sample dilution method, and belongs to the technical field of trace detection. Comprising the following steps: acquiring the conductivity of a to-be-tested sample, substituting the conductivity into a preset dilution relation function, and obtaining the dilution multiple of the to-be-tested sample by utilizing the dilution relation function; diluting the to-be-tested sample according to the dilution ratio to obtain a diluted sample; and testing the diluted sample to obtain the ion concentration. Therefore, after the to-be-tested sample is taken, the accurate dilution multiple can be obtained only by measuring the conductivity of the to-be-tested sample and substituting the conductivity into the dilution relation function, and the sample is diluted and tested, so that accurate dilution and test of different samples are realized, namely, the different to-be-tested samples are adaptively diluted and tested by adopting the appropriate dilution multiple; therefore, the test data is more accurate, and the test efficiency is higher.

The invention discloses a method for detecting an AAV neutralizing antibody. The method comprises the following steps: preparing a recombinant AAV vector AAV-Gluc carrying a Gaussian luciferase reporter gene; the method comprises the following steps: diluting AAV-Gluc according to a certain dilution step to prepare an AAV-Gluc working solution; diluting the sample to be detected; carrying out cell planking, culturing the target cells to form a uniform cell suspension, and adding the cell suspension into a cell culture plate; mixing the sample diluent with the AAV-Gluc diluent, and carrying out incubation; adding the incubated sample mixed solution into the cell culture plate, and carrying out virus infection and culture; obtaining a cultured cell supernatant, adding a detection solution, and detecting signal intensity; and analyzing and sorting the data to obtain the neutralization titer. The secreting type Gaussian luciferase reporter vector is used, target protein is secreted outside cells after being expressed, and detection is facilitated; the infected supernatant is directly sucked for detection, and the operation is convenient and fast; cell lysis and extraction are not needed, protein loss is reduced, and detection accuracy and precision are improved.

The invention relates to the technical field of instrument measuring range expansion, in particular to an instrument measuring range intelligent expansion optimization method based on sample dilution, which comprises the following steps: acquiring an effective measuring range threshold value through an intelligent sensor, counting relative standard deviation and signal-to-noise ratio of a concentration interval in the measuring range, and acquiring a precision distribution curve; performing original sample test on a to-be-tested sample, directly detecting if a response value is within a range, starting an intelligent prediction model according to pollutant types and matched dilution parameters of the to-be-tested sample if the response value exceeds the range, and predicting a recommended dilution multiple according to initial test signal intensity and the sample; when the prediction confidence is lower than a threshold value, triggering gradient scanning; carrying out parallel sample measurement on the diluent to calculate relative standard deviation, executing two adjacent dilution multiples if the distance difference between the diluent and the segmented boundary is smaller than a threshold value, and carrying out mutual verification through concentration consistency of an overlapping region; and performing full-process detection on different dilution multiples, establishing a dilution correction model, updating a correction coefficient of the dilution multiples, and updating a range boundary and a precision distribution curve.

The invention relates to a specific protein analyzer sample track sample injection step cycle dilution reexamination method. The method comprises the following steps: 1, sucking a sample to be detected, and respectively adding the sample to be detected into a reaction cup and a dilution cup; 3, judging whether the analyzer outputs an effective detection result or not, if yes, executing the step 6, and if not, executing the step 4; 4, the dilution puncture needle sucks the sample diluent and the to-be-detected sample in the dilution cup and distributes the sample diluent and the to-be-detected sample in the dilution cup to the dilution cup in an idle state for dilution, and the diluted to-be-detected sample is obtained; 5, sucking the diluted to-be-detected sample into the idle reaction cup by the sample adding needle, and executing the step 2; 6, emptying and cleaning the reaction cup and the dilution cup corresponding to the to-be-detected sample, according to the scheme, the dilution and re-detection process of the sample does not need to be manually intervened, when the detection result is not output, the sample can be automatically diluted and re-detected through the dilution puncture needle and the sample adding needle, the re-detection time is greatly shortened, and the detection efficiency is improved.

This invention discloses a direct bilirubin assay kit and its usage method. The kit includes reagent A, reagent B, and reagent C. Reagent A contains sulfuric acid; reagent B contains 2,5-diethoxy-4-(4-morpholino)diazobenzene sulfate and sulfuric acid; and reagent C contains N,N-diisopropylethylamine and n-hexane. Using this kit, the upper limit of linearity reaches 500 μmol / L, making it suitable for the determination of higher concentrations of direct bilirubin. When measuring high-concentration samples, sample dilution is not required, reducing operational steps and minimizing the influence of human factors on the results. This kit shows no significant interference in DBIL determination when hemoglobin concentration is ≤4.5 g / L, and no significant interference in DBIL determination when triglyceride concentration is ≤40 mmol / L (P>0.05). This indicates that the kit is more suitable for the detection of lipemia and hemolyzed samples.

The utility model relates to the technical field of experimental tools, in particular to a multifunctional kit which comprises a kit body and a kit cover, a clamping support is arranged in the kit body, a plurality of clamping grooves used for clamping and storing reagents are formed in the clamping support, an auxiliary chuck is detachably assembled at an opening of the kit body, and a plurality of clamping holes are formed in the auxiliary chuck in an array mode. The clamping hole is used for keeping the tubular container in a vertical state, the tubular container comprises an EP tube and / or a cryopreservation tube, the box cover is detachably assembled on the peripheral edge of the auxiliary chuck, and the auxiliary chuck and the clamping support are arranged in parallel in a vertically spaced mode. According to the multifunctional kit, a worker can vertically insert an EP tube, a cryopreservation tube and other tubular tools into the clamping holes, reagents / samples in the tube can be conveniently stored or taken, during detection, the worker does not need to additionally prepare an EP tube frame or a sample tube frame or other experiment tools, standard substance preparation, sample dilution and other operations can be directly carried out, operation is more convenient and faster, and the working efficiency is improved. The detection efficiency is improved, and the experiment cost is reduced.

The invention relates to the technical field of cell packaging and counting, in particular to a micro-fluidic chip and a cell counting system. A sample hole, a dye hole, a waste liquid hole and a dilution hole are formed in the micro-fluidic chip, a sample micro-channel, a dye micro-channel, a waste liquid channel and a dilution micro-channel are arranged in the micro-fluidic chip, the sample hole is communicated with the sample micro-channel, the dye hole is communicated with the dye micro-channel, the waste liquid hole is communicated with the waste liquid channel, and the dilution hole is communicated with the dilution micro-channel; a dilution cavity, a uniform mixing micro-channel and a photographing observation cavity are also arranged in the micro-fluidic chip. According to the micro-fluidic chip disclosed by the invention, through integrated design and ingenious design of the positions and connection relations of the dilution cavity, the uniform mixing micro-channel and the photographing observation cavity, the steps of sample dilution, dyeing, observation and the like are successfully integrated into one micro-fluidic chip, so that a full-automatic process from sample introduction to final counting is realized, the operation flow is greatly simplified, and the working efficiency is improved. And the cell counting efficiency and accuracy are greatly improved.

The invention provides a system for testing the content of iron with different valence states in mineral aggregate transfer leaching liquid. The system comprises a sample dilution unit, an auxiliary agent unit and a sample analysis unit, the sample diluting unit is used for diluting a sample to be detected to obtain a diluted sample; the auxiliary unit is used for providing reaction liquid and an indicator; the feeding end of the sample analysis unit is respectively connected with the discharging end of the sample dilution unit and the discharging end of the auxiliary agent unit, and is used for enabling a diluted sample from the sample dilution unit and a reaction liquid and an indicator from the auxiliary agent unit to be subjected to valence state conversion reaction and chromogenic reaction of corresponding valence state iron in sequence; and carrying out ultraviolet irradiation and analysis on the obtained chromogenic reaction mixed solution to obtain the content value of the iron in the corresponding valence state. According to the method and the system, the content of iron with different valence states in the transfer leaching liquid in the wet metallurgy mineral aggregate transfer leaching process can be simply, conveniently and accurately tested on line in real time.

The invention relates to the technical field of medical instruments, and discloses a medical examination liquid adding diluter which comprises a device body, a sealing cover is fixedly connected to the top of the device body, a liquid inlet pipe is fixedly connected to the side face of the device body, and a fixing disc is rotationally connected to the device body and fixedly connected with an output shaft of a motor. The top of the fixed disc is fixedly connected with a liquid distribution disc, and the liquid distribution disc is fixedly connected with the device main body; the device body comprises a device shell, a driving motor is fixedly connected into the device shell, a driving gear is fixedly connected to a top output shaft of the driving motor, and a planet carrier is connected to the side face of the driving gear in an engaged mode. The gear transmission system arranged in the device realizes accurate proportioning and efficient mixing of a diluent and a mother solution, greatly shortens the dilution time of a single sample, is suitable for a scene in which a large number of samples need to be detected every day, can accelerate the dilution process through continuous automatic operation, and improves the flux and timeliness of the whole detection work.

A pouch for a diagnosis item includes (i) a pocket portion with a storage space configured to hold a buffer that has an elongate shape and is configured to hold a sample diluent, and (ii) a buffer supporter formation portion that is connected to one side of the pocket portion. The buffer supporter formation portion includes a buffer support hole that is sized to receive the buffer and support the buffer such that the buffer is oriented in an upright state to avoid spilling of the sample diluent. The buffer supporter formation portion includes a shelf supported by leg portions. The shelf includes the buffer support hole. The buffer supporter formation portion is configured to allow bending of the buffer supporter formation portion into a U shape, and to hold the U shape after being detached from the pocket portion.

A system and method to extend a dynamic range of fluorescence measurements. The system includes a neutral density filter stack apparatus including a plurality of neutral density filters. The neutral density filter stack modulates the intensity of light from a source. This modulation improves the ability to detect emissions from a wider-range of fluorophore concentrations, specifically addressing inner-filter effects at higher concentrations that would normally require sample dilution. It can be used in a system in which absorbance and fluorescence measurements are carried out, wherein the intensity of the light source is modulated with the apparatus for the fluorescence measurements. In this hybrid system, the stack modulates the intensity of the excitation light source for fluorescence measurements to be made at very low concentrations of analyte. The hybrid system also includes optics for absorbance measurements to be made at concentrations of analyte that are higher than those detected by fluorescence.

A method for determining a dilution factor of a sample comprising an analyte (115) of interest is disclosed. The method comprises the following steps:a) (136) preparing a mixture (113) by diluting defined volumes of calibrators (114) comprising the analyte (115) of interest with a defined volume of a dilution reagent comprising at least one first internal standard ISTDa (118);b) (138) performing at least one sample preparation workflow (120) on a defined volume of the mixture (113) thereby generating a prepared mixture (122), wherein the sample preparation workflow (120) comprises adding at least one second internal standard ISTDb (124);c) (140) performing at least one sample measurement on the prepared mixture (122) comprising determining a peak area for each of the first internal standard ISTDa (118) and the second internal standard ISTDb (124) using an in vitro diagnostic medical device (110) and determining an area ratio ARsample of ISTDa (118) and ISTDb (124) by using at least one processing device (130);d) (142) performing at least one calibration measurement on a plurality of calibration samples (128), wherein the calibration samples (128) have a predefined concentration of ISTDa (118) and ISTDb (124), and determining an averaged area ratio ARcalibration,av of ISTDa (118) and ISTDb (124) of the calibration samples (128) by using the processing device (130);e) (144) determining the dilution factor by determining a deviation of the area ratio ARsample and the averaged area ratio ARcalibration,av by using the processing device (130).

The multi-matrix PFAS detection method comprises the following steps: mixing WAX and an HLB (Hydrophile-Lipophile Balance) adsorbent, and then adding a cross-linking agent, an initiator and a catalyst for polymerization reaction to prepare an adsorption glue solution; and filling the adsorption glue solution into a pipe seam between the sampling pipe and the auxiliary pipe, and forming, soaking and cutting to complete the preparation of the annular adsorption phase. Adding an internal standard into a to-be-detected sample, diluting the to-be-detected human body sample with ultrapure water, and not diluting the to-be-detected sewage sample; a to-be-detected sample PFAS is enriched by using a sampling tube containing an annular adsorption phase, and a to-be-detected solution is obtained through elution, nitrogen blowing, redissolution and filtration. The WAX adsorbent and the HLB adsorbent are combined with the cross-linking agent, so that the contact area and the adsorption efficiency of the adsorption material to PFAS are improved, and the adsorption material is suitable for multiple matrixes such as serum, urine and sewage; sample dilution can reduce protein interference and simplify pretreatment steps; by combining the high performance liquid chromatography-tandem mass spectrometry, the PFAS component and content of the sample can be accurately detected, the materials are easy to obtain, and the cost is low.

The present invention relates to a method for determining the boron-gallium ratio of silicates, comprising the following steps: (1) digesting a sample using a hydrolyzed Na2O2 alkali fusion method to prepare a sample mother solution; (2) preparing a sample dilution solution and a sensitivity drift correction solution; (3) preparing a calibration curve standard solution using a hydrolyzed Na2O2 solution and a nitric acid mixed solution; and (4) performing a plasma mass spectrometry test to obtain the boron-gallium ratio of the sample. The determination method of the present invention uses the mixed sample dilution solution as a sensitivity drift correction solution to perform instrument signal float correction, and a standard solution matching the matrix of the sample dilution solution for calibration, thereby avoiding the different matrix effects of boron and gallium caused by different media and solving the problem of reagent background subtraction, thereby achieving the effect of efficiently and accurately testing the boron-gallium ratio.

The application provides an incubation device for immunohistochemistry, a staining treatment all-in-one machine and a control method thereof. The device comprises a cabin body, a carrier and a condensation flow guide plate. The carrier is provided with a humidification flow channel, which is divided into multiple liquid accumulation pools by a liquid distribution baffle, and the overflow principle is used to ensure uniform humidity and inhibit liquid accumulation during movement. The condensation flow guide plate is provided with a heating surface and a condensation surface, the temperature of the condensation surface is controlled to be lower than the dew point, vapor is induced to condense on the condensation surface and is discharged to a waste liquid outlet through the flow guide surface, and condensate droplets are prevented from falling on the slide. A driving assembly drives the carrier to move between an incubation position and an operation position and drives the cabin door to move. The application combines zoned temperature control, induced condensation and physical directional flow guidance, solves the risk of sample dilution caused by random condensation of condensate in a high humidity environment, simplifies the overall structure of the machine and improves the automation integration degree.

This application discloses a sample dilution method, a detection method, a detection device, and a storage medium. The sample dilution method includes: estimating at least one dilution ratio; determining another dilution ratio based on the target dilution ratio of the sample and the at least one dilution ratio; adjusting the at least one dilution ratio until the other dilution ratio meets a preset requirement, and determining that the current at least one dilution ratio and the other dilution ratio are respectively the dilution ratios used in one dilution process within a series of dilutions of the sample. Through the above method, this application can improve the accuracy of the dilution ratio of the target solution when performing multiple dilutions.

The invention belongs to the technical field of detection, and particularly discloses a measurement uncertainty evaluation method for measuring immune factors in serum by an ELISA (enzyme-linked immuno sorbent assay) method. According to the method, ELISA detection is carried out on immune factors (such as IL-1beta) in serum of an experimental animal in contact with a medical instrument according to standards such as GB / T16886.20-2015, system analysis comprises nine types of uncertainty sources such as repeated detection, standard substance preparation and sample dilution, the standard uncertainty of each component is calculated through mathematical methods such as rectangular distribution and a least square method, and the standard uncertainty of each component is calculated according to the standard uncertainty. And synthesizing the standard uncertainty and calculating the expansion uncertainty, and finally outputting a standard detection result. The method comprehensively covers the uncertainty source, is rigorous in computational logic and clear in key quality control factors, provides a scientific and complete uncertainty evaluation scheme for medical instrument immunogenicity evaluation and immune factor determination in other experimental scenes, is beneficial to laboratory quality control and detection result optimization, and has wide practicability.

The utility model belongs to the technical field of auxiliary equipment for food microbiological detection, and discloses a homogenizing bag frame, the homogenizing bag frame is used for placing a homogenizing bag, the homogenizing bag is provided with a positioning hole, the homogenizing bag frame comprises a first connecting piece, support frames and hooks, the first connecting piece and two adjacent support frames form a support frame, and the first connecting piece and the two adjacent support frames form the support frame. A worker aligns the positioning holes with the hooks and puts one homogenizing bag into the corresponding supporting frame, and the other end of each hook is inserted into the corresponding positioning hole to hook the homogenizing bag, so that the homogenizing bags are fixed, the homogenizing bags can be fixed on the hooks in the process of inclining the whole homogenizing bag frame to suck sample diluent in the homogenizing bags, and the sample diluent in the homogenizing bags can be sucked by the hooks. The sample diluent can be sucked more conveniently, and the situation that the sample diluent in the homogenizing bag is spilled out and the loss of the sample diluent is caused due to the fact that the homogenizing bag slides out of the upper portion of the supporting frame is avoided.

The invention discloses application of serum alpha-non-red blood cell ghost protein 1 in preparation of drugs for diagnosing heart light chain amyloidosis diseases, and belongs to the technical field of biological medicines. The serum SPTAN1 protein is used for preparing the medicine for diagnosing the heart light-chain amyloidosis disease, the medicine is limited to be an enzyme linked immunosorbent assay kit, and the kit comprises an antibody of the SPTAN1 protein and further comprises a pre-coated plate, a standard substance, a coating buffer solution, a sample diluent, a confining liquid, horse radish peroxidase, an eluent and a color developing agent. The concentration of the SPTAN1 protein in the serum is measured through an enzyme linked immunosorbent assay kit, and early diagnosis of the heart AL amyloidosis disease is carried out. The invention provides a novel, efficient and sensitive biomarker, namely the serum SPTAN1 protein, early diagnosis of heart AL amyloidosis is realized by detecting the concentration of the SPTAN1 protein in serum, and the serum SPTAN1 protein has an important clinical application prospect.

The invention relates to a pancreatic ductal adenocarcinoma exosome marker detection kit based on a chemiluminescence method as well as a preparation method and application of the pancreatic ductal adenocarcinoma exosome marker detection kit. The kit comprises an exosome targeted capture processing component, an REG1A detection component, an REG1B detection component and a common component, the exosome targeted capture treatment component consists of EphA2 immunocapture magnetic beads, a high-efficiency capture buffer solution and an exosome lysis solution; the REG1A detection component is composed of REG1A capture magnetic particles, a horse radish peroxidase labeled anti-REG1A detection antibody, a REG1A calibration product and a REG1A quality control product; the REG1B detection component is composed of REG1B capture magnetic particles, a horse radish peroxidase labeled anti-REG1B detection antibody, a REG1B calibration product and a REG1B quality control product; the kit is composed of a 20 * concentrated washing solution, a sample diluent and a chemiluminescent substrate solution. The detection kit can effectively eliminate interference of free protein and other exosomes, the detection specificity reaches 98.3%, the sensitivity reaches 1.6-1.9 pg / mL, and a more reliable auxiliary detection basis is provided for clinical decision making.

The utility model relates to a detection sample dilution system, which comprises a reaction cup, a first pump body and a sampling needle, the reaction cup mixes a sample and a diluent, and fully shakes up to obtain a first mixed solution; the reaction cup is also used for discharging the residual first mixed solution; mixing the first mixed solution and the diluent in the reaction cup, and fully shaking up to obtain a second mixed solution; the first pump body is communicated with the reaction cup; the first pump body pumps the diluent into the reaction cup; the first pump body pumps a certain amount of first mixed liquid in the reaction cup for temporary storage; after the residual first mixed solution is discharged from the reaction cup, a certain amount of the first mixed solution is introduced into the reaction cup again; the first pump body injects a diluent into the reaction cup; the sampling needle extracts a sample and injects the sample into the reaction cup; the sampling needle injects the reagent into the reaction cup. Compared with the prior art, the rapid dilution device can rapidly dilute a sample solution, the dilution multiple is flexible and adjustable, the sample testing efficiency is improved, and the sample dilution cost can be reduced.