442 results about "Sample dilution" patented technology

The present invention provides methods and apparatuses related to measurement of analytes, including measurements of analytes in samples withdrawn from a patient.

The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

The invention is to devices and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature for forming a diluted sample for analysis. The devices and methods also include a dilution verification feature for verifying the degree of dilution of the diluted sample. The devices preferably are capable of being used in the point-of-care diagnostic field is provided.

In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample extraction unit, wherein a portion of said extraction unit defines a metered volume of a sample. A diluent may be transported over and/or through the extraction unit to form a diluted sample for sample analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, capable of being used in the point-of-care diagnostic field is provided. The devices and methods of the invention preferably are well-suited for high range sample dilution.

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent/stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.

The present invention relates to a method for the highly sensitive Immuno-PCR detection of an analyte in a sample comprising the use of a nucleic acids containing sample dilution buffer for diluting the sample as well as methods for the preparation of the sample dilution buffer and the use thereof.

The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.

A method of liquid chromatography includes providing an injection valve, drawing a sample and a diluent while mixing, pushing the mixed sample and diluent onto a sample loop of the injection valve, and injecting the mixed sample and diluent. An analytical apparatus includes a proportioning unit, an injection valve having a sample loop, and a sample pump. The injection valve has a draw state and a load state, and has a port in fluidic communication with an outlet port of the proportioning unit. The sample pump is in fluidic communication with the outlet port of the proportioning unit, if the injection valve is in the draw state, to draw both a sample and a diluent through the proportioning unit and the injection valve, and in fluidic communication with the sample loop, if the injection valve is in the load state, to push the drawn sample and diluent onto the sample loop.

A discharge ionization current detector capable of supplying plasma gas in large quantity to stabilize plasma simultaneously with lowering the sample dilution ratio to improve detection sensitivity is provided. A gas supply pipe 7 for supplying a plasma gas, which also functions as a dilution gas, is connected to a point near the connecting section of a first gas passage 3 having electrodes 4-6 for plasma generation and a second gas passage having electrodes 16 and 17 for ion detection. A first gas discharge pipe 8 is connected to the other end of the first gas passage 3, and a second gas discharge pipe 13 is connected to the other end of the second gas passage 11. Flow controllers 9 and 14 are provided in the gas discharge pipes 8 and 13, respectively. The flow rate of the gas passing through a plasma generation area and that of the gas passing through an ion current detection area can be independently regulated. Therefore, for example, it is possible to increase the former flow rate to stabilize the plasma and simultaneously decrease the latter flow rate to enhance the detection sensitivity for a low-concentration sample.

The invention relates to a kit for quickly and quantificationally detecting serum amyloid A (SAA) by using an immunogold method. The kit includes lyophilized anti-SAA immunogold, a reaction plate, a sample diluent, a lyophilized colloidal gold complex solution, a scrubbing solution and a sealing solution. The preparation method comprises the following steps: (1) firstly preparing a conjugate of the colloidal gold and anti-SAA immunogold, and preparing lyophilized anti-SAA immunogold; and (2) preparing the sample diluent, the lyophilized colloidal gold complex solution, the scrubbing solution and the sealing solution according to the proportion, preparing the reaction plate, and assembling the prepared objects to a finished product to obtain the kit. By using the kit provided by the invention, a doctor can obtain an SAA measurement result on the spot when a person is ill so as to quickly judge the patient's condition, so that more targeted treatment can be effectively taken on the patient, healing time is shortened and medical cost is reduced. The kit provided by the invention has a good application prospect.

The invention relates to a diagnostic reagent kit for testing the hepatitis c virus (HCV) and the preparation and test method, which is to add the HCV recombinant antigen used for peridium into the buffer solution, blend it, move into the luminous microplate, make incubation for 18 hours under 4DEG.C, wash the luminous microplate, add into the confining liquid, leave the liquid after incubation and fully dry the luminous microplate to complete the preparation of the pre-peridium luminous microplate; combine the anti-human IgG used for marking and the horse radish peroxidase by improving the sodium periodate to complete the preparation of the enzyme marker; prepare the chemical luminous substrate solution A with luminal, Tween20 and luminous intensifier and prepare the chemical luminous substrate solution B with the hydrogen peroxide. The reagent kit also comprises the sample diluent and concentrated scrub solution. The negative corresponds to the normal human serum while the positive corresponds to the people with serum of pooled serum with HCV antibody. The reagent kit provided in the invention has much higher detection sensitivity than the ELISA, which is safe and reliable, easy to operate with low cost, and without any expensive full-automatic chemical luminous measuring apparatus required.

In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample isolation unit and having a diluent introduction port disposed therebetween for introduction of a diluent into the sample holding chamber. The volume within the sample holding chamber between the diluent introduction port and the sample isolation unit defines a metered volume of a sample for analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, preferably suitable for low range sample dilution, and preferably capable of being used in the point-of-care diagnostic field.

The invention relates to an active sludge online computer image analysis and early warning system and an active sludge online computer image analysis and early warning method thereof. The system consists of a sample diluter, a peristaltic pump, a computer, a high-speed camera, a microscope and a sample chamber. The method comprises the steps of acquiring a morphological image of a sample firstly and then processing the acquired image; analyzing floc size distribution and filamentous bacterium length distribution; associating the floc particle size distribution and filamentous bacterium length distribution of active sludge with settling properties of the active sludge so as to perform early warning on the settling effect of the active sludge.

The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.

The invention discloses a portable underground water fixed-depth sampling device, a sampling system and a sampling method, and belongs to the field of site pollution investigation and risk assessment.A moving blocking air bag is arranged; stagnant water at the upper end and the lower end of an underground water sampling probe in a well pipe is effectively blocked, an underground water sample at the depth where the sampling probe is located can be accurately collected, the problem that in the well washing sampling process of a traditional method, upper-layer water samples and lower-layer watersamples in the well pipe are mixed, and consequently sample dilution is caused is solved, and the concentration detection accuracy is improved. Underground water samples of different depths can be accurately collected in the same monitoring well, fixed-depth sampling is achieved, and the cost is obviously reduced. In addition, the stagnant water on the upper layer and the lower layer of the underground water sampling probe in the well pipe is effectively blocked, the well washing volume before sampling can be reduced, the sampling time is saved, the sampling efficiency is improved, the well washing wastewater generation amount is reduced, the sampling cost is further reduced, disturbance to underground water in the well pipe can be reduced, and the accuracy of detected concentration is guaranteed.

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

The invention provides a liquid chromatographic sampling passage cleaning device. The liquid chromatographic sampling passage cleaning device mainly comprises a microfluidic pump, a compressed air source, a capillary flow limiter, a liquid-gas switching valve, a sampling-cleaning switching valve, a waste liquid collecting cup, a waste liquid bottle, a sampling valve, a sampling port and a program controller. After sampling, a cleaning solution is used for reversely rinsing a liquid chromatographic sampling passage to reversely rinse particles left on a filter in the sampling process and discharge the particles out of a sampling pipeline first and then microflow gas is used for reversely purging the sampling passage to expel liquid left inside the pipeline so as to avoid sample dilution during sampling once again. The liquid chromatographic sampling passage cleaning device is suitable for various analytical-grade liquid chromatographs, comprising positive chromatography, reverse chromatography, ion chromatography and the like, as well as suitable for cleaning a sampling loop for flow injection analysis, so that cross pollution of samples is avoided, the sampling repeating precision is improved, and the service life of the sampling filter can be greatly prolonged.

The invention discloses a stationary combustion source flue gas mixing channel dilution multistage sampling device, and relates to a stationary combustion source emission high temperature flue gas dilution cooling device, physical and chemical change processes in the process for emission of high temperature flue gas into environment atmosphere are simulated by the device, an automatic feedback regulation controlled two-stage dilution channel is used, sampling dilution ratio and residence time can be adjusted, accurate dilution ratio and temperature control can be achieved, experimental data acquisition, recording and display can be achieved, eight particle size levels of atmospheric total suspended particles in the flue gas are simultaneously sampled, and the device belongs to the technical field of environmental detection. The stationary combustion source flue gas mixing channel dilution multistage sampling device comprises a sampling gun, a heating insulating tube, a jet pump, a dilution chamber, a clean air generator, a temporary storage box, a multistage sampling system and a display control system in effective assembly to complete high temperature flue gas two-stage dilution sampling. The stationary combustion source flue gas mixing channel dilution multistage sampling device effectively solves the problem that first condensation particles in stationary combustion source emission flue gas are difficult to collect, and can be used for classified sampling of particles in the stationary combustion source emission flue gas, the whole device adopts a modular design, is easy to repair, maintain and service, and the whole device can be integrated with a mobile platform, and is suitable for in-situ sampling work under various conditions.

The invention relates to a kit for determining troponin-T (TNT) content of serum, aims to overcome the deficiencies of the above background technology, and provides a kit for detecting troponin-T by an immunoturbidimetry method, wherein the kit does not require sample dilution, has simple operation, high accuracy and good repeatability, and is suitable for various types of fully automatic biochemical analyzers and special protein instruments. A technical scheme is as below: the kit comprises: A. a reagent R1 containing a buffer solution, a preservative, an accelerating agent, inorganic salts, a surfactant and the balance of purified water; b. a reagent R2 containing a buffer solution, latex microspheres with diameter of 60-150 nm combined with anti-human troponin antibodies and an antiseptic; and c. a reference calibrator containing a buffer solution, a stabilizer, a preservative, a certain amount of recombinant human protein troponin-T pure product determined by concentration requirements and the balance of purified water.

The invention discloses an apolipoprotein E4 ELISA kit and a preparation method thereof, the invention applies the purified human apolipoprotein E4 to prepare an anti-human ApoE4 antibody, and an ELISA plate is coated after the compatible purification. The kit composition includes the ELISA plate coated by the anti-human ApoE4 monoclonal antibody, an enzyme-labeled antibody, ApoE4 standard frozen powder, sample dilution solution, TMB substrate color developing solution, concentration washing liquid and reaction termination liquid. The ApoE4 in the antibody capture standard solution or the sample solution which is fixed on the microporous surface of the ELISA plate is identified by and combined with the enzyme-labeled antibody, so as to form the antibody-ApoE4-antibody-enzyme compound, the absorbance is measured after the reaction and color development of the enzyme and the substrate and the concentration of the ApoE4 in the sample can be calculated by the standard curve. The kit can detect the consistency of the apolipoprotein E4 in human serum, plasma or cerebrospinal fluid, the invention has the advantages of sensitivity, rapidness, simpleness and accuracy, so the invention provides the effective means for in vitro diagnosis and determination of ApoE4 gene type and the gene dosage.

The invention relates to a preparation and detection method for an ELISA kit detecting Fumonisins. The ELISA kit has the characteristics of sensitive, accurate and fast detection, simple operation and strong specificity, and is suitable for detection of a large number of samples. The kit includes: a Fumonisins antigen coated enzyme label plate, a Fumonisins standard substance, a Fumonisins antibody working solution, a Fumonisins enzyme labeled secondary antibody working solution, a substrate solution A, a substrate solution B, a terminating solution, a concentrated sample dilution solution and a concentrated washing solution. The principle of the Fumonisins detection kit is solid-phase indirect competitive enzyme-linked immunosorbent assay reaction. The extracted sample, the enzyme labeled secondary antibody working solution, and the antibody working solution are added into corresponding enzyme labeled holes, after incubation for a period of time, the substrate solution A and the substrate solution B are added into a washing plate, and under the action of enzyme, the holes can present a blue color. Then the terminating solution is added, and the color changes to yellow from blue. The color depth and the content of Fumonisins in the standard substance or the sample are in an inverse proportion relationship. This method can be directly used for detecting Fumonisins in maize.

The invention discloses a method for measuring content of chlorine in a mixed acid electrolyte through potentiometric titration. According to the method, the chlorine content of a to-be-measured solution is titrated by adopting a silver nitrate standard solution, a titration end point is judged according to change of electric potential in a titration process, and the content of chloride ions in a tested sample is calculated according to the volume of the consumed silver nitration solution. By means of sample dilution and addition of an appropriate amount of nitric acid in a sample processing process, on one hand, the influence of other acid radicals in the sample on the titration is eliminated; on the other hand, trivalent vanadium ions in the solution are oxidized into tetravalent vanadium ions through addition of nitric acid, so that complexing capacity between the vanadium ions and the chloride ions is reduced, and the reaction of titrating the chloride ions by silver nitrate is enabled to be more smooth and more stable. According to the chloride ion concentration obtained by the test, a chloride ion masking agent is added through calculation, and the content of trivalent vanadium and tetravalent vanadium in the solution is measured with a potassium permanganate-potentiometric titration method. The method effectively avoids disturbance of the chloride ions on the vanadium measurement process and is fast and accurate.