Biological sample diluting and cultivating device
By designing a biological sample dilution and cultivation device, the problem of uneven sample dilution is solved by using group dilution and sealing structure, uniform dilution and anaerobic cultivation of samples are achieved, and experimental accuracy is improved.
Patent Information
- Application Number
- CN202422193252.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-09
- Publication Date
- 2025-08-01
- Estimated Expiration
- 2034-09-09
AI Technical Summary
In the prior art, it is difficult to ensure uniform mixing of samples and dilution during dilution and cultivation of biological samples, especially viscous samples, which affects the accuracy of experimental results.
A biological sample dilution and cultivation device is designed, which includes a culture tank composed of an upper cover, a stress strip, a seal strip and a plurality of baffles. Through group dilution and sealing structures, the samples are ensured to be evenly mixed and cultivated in an oxygen-free environment.
The uniform dilution of the samples is achieved, the accuracy of experimental results is improved, and bacterial samples can be cultivated in an aerobic environment.
Smart Images
Figure CN223176095U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of sample cultivation, in particular to a biological sample dilution and cultivation device. Background Art
[0002] Dilution and cultivation of biological samples can usually be carried out according to the following steps: Dilution operation: Dilution operation is carried out in an ultra-clean workbench or sterile environment to prevent external microorganisms from contaminating the sample; operators should wear sterile laboratory coats, gloves, masks, etc.; Take a certain amount of original sample and add it to a test tube containing an appropriate amount of sterile diluent (such as saline, buffer, etc.), mix it thoroughly to obtain the first dilution; Then take a certain amount from the first dilution and add it to the next test tube containing dilution, and so on, to obtain sample solutions with different dilution multiples; Cultivation operation: Use a pipette to draw up an appropriate amount of diluted sample and inoculate it onto a pre-prepared culture medium; Different inoculation methods such as coating method and pouring method can be used; Try to avoid bubbles and splashing during the operation; Set appropriate culture conditions according to the characteristics of the biological sample, including temperature, humidity, oxygen content, etc.; For example, bacterial culture is usually carried out in a constant temperature incubator at about 37°C, while some cell cultures may require a specific carbon dioxide concentration; Observe the growth of the sample regularly during the culture process, and record characteristics such as growth rate and morphological changes;
[0003] When performing dilution operations in a culture dish, it may be difficult to ensure that the sample and diluent are fully and evenly mixed, resulting in local concentrations that are too high or too low, affecting the accuracy of the experimental results; especially for viscous biological samples, it is even more difficult to achieve uniform dilution. Utility Model Content
[0004] The purpose of the present invention is to provide a biological sample dilution and incubation device to solve the defects mentioned in the above background technology.
[0005] To achieve the above-mentioned objectives, a biological sample dilution and incubation device is provided, comprising an upper cover, a stress-bearing strip fixedly mounted on the outer side of the upper cover, and a sealing strip fixedly mounted on the circumferential inner wall of the upper cover, a culture dish mounted on the bottom of the upper cover, and a positioning area provided on the outer side of the culture dish, a first baffle fixedly arranged inside the culture dish, a second baffle mounted on one side of the first baffle, and a third baffle mounted on one side of the second baffle, and both ends of the third baffle, the second baffle, and the first baffle are all fixedly mounted on the inner wall surface of the culture dish; a first incubation groove is formed between the first baffle and the side wall of the culture dish, a second incubation groove is formed between the second baffle and the side wall of the culture dish, a third incubation groove is formed between the third baffle and the side wall of the culture dish, and a fourth incubation groove is formed between the first baffle, the second baffle, the third baffle, and the side wall of the culture dish.
[0006] Preferably, two groups of stress bars are fixedly provided on the circumferential outer surface of the upper cover, and the cross section of the stress bars is a semicircular structure, and the stress bars and the upper cover are a concentric circle structure.
[0007] Preferably, a sealing groove is provided on the circumferential outer wall surface of the culture dish, and the size of the sealing groove matches that of the sealing strip, and the cross-sections of the sealing strip and the sealing groove are both isosceles trapezoidal.
[0008] Preferably, the upper cover is mounted inside the sealing groove on the outer surface of the culture dish via an internal sealing strip, and the sealing strip is an annular structure made of rubber material.
[0009] Preferably, a stress-bearing inclined surface is provided on the outer side of the top of the culture dish, and the inclination of the stress-bearing inclined surface is consistent with the inclination of the bottom inclined surface of the sealing strip.
[0010] Preferably, the positioning area is adapted to the size of the upper cover, and the bottom of the upper cover is clamped on the outer surface of the positioning area.
[0011] Compared with the prior art, the beneficial effects of the present invention are:
[0012] 1. In this utility model, biological samples to be cultured can be evenly divided into four groups and placed in the first, second, third, and fourth culture tanks in sequence. During dilution, the samples in the four groups of culture tanks can be diluted separately, reducing the amount of sample required for dilution in each group and improving the uniformity of sample dilution. This device can also be used to observe the growth of samples at different dilution concentrations.
[0013] 2. In the present invention, the inclination rate of the force-bearing inclined surface is consistent with the slope of the bottom inclined surface of the sealing strip, so that the rubber sealing strip is squeezed and moves along the outer wall of the culture dish. When the squeezed sealing strip moves to a relative position with the sealing groove, the compressed sealing strip resets and rebounds, so that the sealing strip is stuck inside the sealing groove, which can seal the culture dish and facilitate the cultivation of bacterial samples in an oxygen-free environment. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 This is a front view schematic diagram of the structure of the utility model;
[0015] Figure 2 for Figure 1 Bottom view of
[0016] Figure 3 for Figure 1 A top view of
[0017] Figure 4 A cross-sectional view of a culture dish.
[0018] Reference Numerals in the Figures: 1. Upper cover; 2. Stress bar; 3. Sealing strip; 4. Culture dish; 41. First baffle; 42. Second baffle; 43. Third baffle; 5. Stress inclined plane; 6. Sealing groove; 7. Positioning area. Detailed implementation mode
[0019] Please refer to Figures 1-4 , the present utility model provides a biological sample dilution and culture device, including an upper cover 1, a stress bar 2 is fixedly installed on the outer side of the upper cover 1, a sealing strip 3 is fixedly installed on the circumferential inner wall of the upper cover 1, a culture dish 4 is installed at the bottom of the upper cover 1, a positioning area 7 is opened on the outer side of the culture dish 4, a first baffle 41 is fixedly arranged inside the culture dish 4, a second baffle 42 is installed on one side of the first baffle 41, a third baffle 43 is installed on one side of the second baffle 42, and both ends of the third baffle 43, the second baffle 42 and the first baffle 41 are fixedly arranged on the inner wall surface of the culture dish 4.
[0020] Working principle: A first culture tank is formed between the first baffle 41 and the side wall of the culture dish 4, a second culture tank is formed between the second baffle 42 and the side wall of the culture dish 4, a third culture tank is formed between the third baffle 43 and the side wall of the culture dish 4, and a fourth culture tank is formed between the first baffle 41, the second baffle 42, the third baffle 43 and the side wall of the culture dish 4; the biological samples to be cultured can be evenly divided into four groups and sequentially placed into the first culture tank, the second culture tank, the third culture tank and the fourth culture tank. When diluting, the samples in the four culture tanks can be diluted in groups respectively, reducing the sample content to be diluted in each group and improving the uniformity of sample dilution; at the same time, samples with different dilution concentrations can also be diluted in the first culture tank, the second culture tank, the third culture tank and the fourth culture tank, expanding the use range of the culture dish 4. With this device, the growth of samples under different dilution concentrations can be observed; when culturing samples that require an anaerobic environment, at this time, hold the stress bar 2 on the outer side of the upper cover 1, and the stress bar 2 facilitates the installation or disassembly of the upper cover 1. Press down the upper cover 1, so that the sealing strip 3 on the inner wall surface of the upper cover 1 contacts the stress inclined plane 5 on the top of the culture dish 4. The inclination rate of the stress inclined plane 5 is the same as the slope of the bottom inclined plane of the sealing strip 3, so that the rubber sealing strip 3 is squeezed and moves on the outer side wall of the culture dish 4. When the squeezed sealing strip 3 moves to a relative position with the sealing groove 6, the compressed sealing strip 3 rebounds and resets, so that the sealing strip 3 is clamped inside the sealing groove 6, which can seal the culture dish 4 and facilitate the cultivation of bacterial samples in an anaerobic environment.
[0021] Two groups of stress bars 2 are fixedly arranged on the circumferential outer surface of the upper cover 1, the cross-section of the stress bar 2 is a semi-circular structure, and the stress bar 2 and the upper cover 1 are concentric structures.
[0022] As a preferred embodiment, a sealing groove 6 is formed on the circumferential outer wall surface of the culture dish 4, and the size of the sealing groove 6 is adapted to that of the sealing strip 3. At the same time, the cross-sections of both the sealing strip 3 and the sealing groove 6 are isosceles trapezoids.
[0023] The upper cover 1 is clamped inside the sealing groove 6 on the outer surface of the culture dish 4 through the internal sealing strip 3, and the sealing strip 3 is an annular structure made of rubber material.
[0024] A force-receiving inclined surface 5 is formed on the outer side of the top of the culture dish 4, and the inclination rate of the force-receiving inclined surface 5 is consistent with the slope of the bottom inclined surface of the sealing strip 3.
[0025] As a preferred embodiment, the positioning area 7 is adapted to the size of the upper cover 1, and the bottom of the upper cover 1 is clamped on the outer surface of the positioning area 7.
[0026] A first culture groove is formed between the first baffle 41 and the side wall of the culture dish 4, a second culture groove is formed between the second baffle 42 and the side wall of the culture dish 4, a third culture groove is formed between the third baffle 43 and the side wall of the culture dish 4, and a fourth culture groove is formed between the first baffle 41, the second baffle 42, the third baffle 43 and the side wall of the culture dish 4.
Claims
1. A biological sample dilution and cultivation device, comprising an upper cover (1), characterized in that: A stress bar (2) is fixedly installed on the outer side of the upper cover (1), and a sealing strip (3) is fixedly installed on the circumferential inner wall of the upper cover (1). At the same time, a culture dish (4) is installed at the bottom of the upper cover (1), and a positioning area (7) is provided on the outer side of the culture dish (4). A first baffle (41) is fixedly arranged inside the culture dish (4), and a second baffle (42) is installed on one side of the first baffle (41). At the same time, a third baffle (43) is installed on one side of the second baffle (42). At the same time, both ends of the third baffle (43), the second baffle (42), and the first baffle (41) are fixedly arranged on the inner wall surface of the culture dish (4); a first culture groove is formed between the first baffle (41) and the side wall of the culture dish (4), a second culture groove is formed between the second baffle (42) and the side wall of the culture dish (4), a third culture groove is formed between the third baffle (43) and the side wall of the culture dish (4), and a fourth culture groove is formed between the first baffle (41), the second baffle (42), the third baffle (43) and the side wall of the culture dish (4).
2. The biological sample dilution and cultivation device according to claim 1, wherein: Two groups of stress bars (2) are fixedly arranged on the circumferential outer surface of the upper cover (1), and the cross-section of the stress bar (2) is a semi-circular structure. At the same time, the stress bar (2) and the upper cover (1) are concentric circle structures.
3. The biological sample dilution and cultivation device according to claim 1, characterized in that: A sealing groove (6) is provided on the circumferential outer wall surface of the culture dish (4), and the size of the sealing groove (6) is adapted to that of the sealing strip (3). At the same time, the cross-sections of the sealing strip (3) and the sealing groove (6) are both isosceles trapezoid settings.
4. The biological sample dilution and cultivation device according to claim 1, characterized in that: The upper cover (1) is clamped inside the sealing groove (6) on the outer surface of the culture dish (4) through the internal sealing strip (3), and the sealing strip (3) is an annular structure made of rubber material.
5. A biological sample dilution and cultivation device according to claim 1, characterized in that: A stress inclined surface (5) is provided on the outer side of the top of the culture dish (4), and the inclination rate of the stress inclined surface (5) is the same as the slope of the bottom inclined surface of the sealing strip (3).
6. The biological sample dilution and cultivation device according to claim 1, wherein: The positioning area (7) is adapted to the size of the upper cover (1), and the bottom of the upper cover (1) is clamped on the outer surface of the positioning area (7).