A sample dilution method, a detection method, a detection device, and a storage medium.

Abstract

This application discloses a sample dilution method, a detection method, a detection device, and a storage medium. The sample dilution method includes: estimating at least one dilution ratio; determining another dilution ratio based on the target dilution ratio of the sample and the at least one dilution ratio; adjusting the at least one dilution ratio until the other dilution ratio meets a preset requirement, and determining that the current at least one dilution ratio and the other dilution ratio are respectively the dilution ratios used in one dilution process within a series of dilutions of the sample. Through the above method, this application can improve the accuracy of the dilution ratio of the target solution when performing multiple dilutions.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical treatment, in particular to a sample dilution method, a detection method, a detection device and a storage medium. BACKGROUND

[0002] At present, medical examination technology can realize automatic analysis of various cells or biochemical components in blood, and obtain indexes of each component in blood. When performing blood cell analysis, such as analyzing red blood cells or platelets, the blood is usually diluted to a certain multiple, and then blood cell counting is performed.

[0003] In order to realize accurate analysis of blood, the sample usually needs to be diluted to a lower concentration, which may cause the sample and the diluent to exceed the range and accuracy of the sampling needle, and finally result in a large error between the obtained sample dilution ratio and the target dilution ratio. SUMMARY

[0004] The present application mainly provides a sample dilution method, a detection method, a detection device and a storage medium, which can solve the problem that the sample suction amount and the diluent suction amount are difficult to balance in multiple dilutions in the prior art.

[0005] To solve the above technical problems, the present application provides a sample dilution method in the first aspect. The method comprises the following steps: estimating at least one dilution ratio; determining another dilution ratio according to the target dilution ratio of the sample and the at least one dilution ratio; adjusting the at least one dilution ratio until the other dilution ratio meets the preset requirement, and determining the at least one dilution ratio and the other dilution ratio as the dilution ratio used in one dilution process in the multiple dilution processes of the sample respectively.

[0006] To solve the above technical problems, the present application provides a sample detection method in the second aspect. The method comprises: determining a first dilution ratio and a second dilution ratio according to the method provided in the first aspect; based on the second dilution ratio, sucking diluent and sample liquid, and spitting the sucked liquid into a first dilution cup to obtain a first solution; based on the first dilution ratio, sucking diluent and the first solution, and spitting the sucked liquid into a second dilution cup to obtain a second solution; and detecting the second solution.

[0007] To solve the above technical problems, the present application provides a sample detection device in the third aspect. The sample detection device comprises a processor and a memory coupled to each other. The memory stores a computer program, and the processor is configured to execute the computer program to realize the sample dilution method provided in the first aspect.

[0008] To address the aforementioned technical problems, a fourth aspect of this application provides a computer-readable storage medium storing a computer program that, when executed by a processor, implements the sample dilution method provided in the first aspect.

[0009] The beneficial effects of this application are as follows: Unlike existing technologies, this application first estimates at least one dilution ratio, then uses the target dilution ratio and the estimated at least one dilution ratio to determine another dilution ratio. It then judges whether the obtained dilution ratio meets the preset requirements. If it does not, the estimated at least one dilution ratio is adjusted, and another dilution ratio is determined again, until the preset requirements are met. By continuously adjusting the estimated dilution ratio, the dilution ratio value used in each dilution is made more consistent with the preset requirements. In this way, the optimal dilution ratio for each dilution step can be found, reducing errors caused by inaccurate sample and diluent addition volumes due to dilution ratio data, and making the final sample dilution ratio more consistent with the target dilution ratio. Attached Figure Description

[0010] Figure 1 This is a schematic flowchart of an embodiment of the sample dilution method of this application;

[0011] Figure 2 This is a flowchart illustrating another embodiment of the sample dilution method of this application;

[0012] Figure 3 This is a schematic flowchart of another embodiment of the sample dilution method of this application;

[0013] Figure 4 This is a flowchart illustrating an embodiment of the preset requirements defined in this application;

[0014] Figure 5 This is a schematic diagram of the structure of an embodiment of the sample dilution device of this application;

[0015] Figure 6 This is a simplified schematic diagram of an embodiment of the dilution process of this application;

[0016] Figure 7 This is a schematic diagram of the structure of an embodiment of the transfer component of this application;

[0017] Figure 8 This is a schematic diagram of an embodiment of the transport path of each component in the dilution process of this application;

[0018] Figure 9 This is an exploded view of an embodiment of the transfer component of this application;

[0019] Figure 10 This application is this application. Figure 9 A schematic diagram of a embodiment of the transfer shaft;

[0020] Figure 11 This is a schematic diagram of another embodiment of the rotating mechanism of this application;

[0021] Figure 12 This is a schematic block diagram of the circuit structure of an embodiment of the sample analyzer of this application;

[0022] Figure 13 This is a schematic flowchart of an embodiment of the detection method for samples in this application;

[0023] Figure 14 This is a flowchart illustrating another embodiment of the detection method for samples in this application;

[0024] Figure 15 This is a flowchart illustrating another embodiment of the detection method for samples in this application;

[0025] Figure 16 This is a schematic block diagram of the circuit structure of an embodiment of the sample detection device of this application;

[0026] Figure 17 This is a schematic block diagram of the circuit structure of an embodiment of the computer-readable storage medium of this application. Detailed Implementation

[0027] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0028] The terms "first" and "second" in this application are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features shown. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to such processes, methods, products, or apparatus.

[0029] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0030] In testing applications requiring the dilution of samples such as blood, the ratio of diluent to sample volume is extremely high when a low-concentration diluent is needed. Attempting to dilute in a single step could result in the diluent volume exceeding the sampling needle's range or the sample volume falling short of the needle's precision, ultimately leading to inaccurate target solution dilution ratios. Therefore, multiple dilution steps can improve the accuracy of the target solution dilution ratio. In each of these multiple dilution steps, the solution obtained in the previous dilution step is used as the sample for further dilution.

[0031] This application proposes a sample dilution method to determine the dilution ratios used in multiple dilutions. Specifically, one dilution ratio is calculated by estimating another dilution ratio. Each dilution process is performed using either one dilution ratio or the other, and after multiple dilutions, a target solution that meets the target dilution ratio is finally obtained.

[0032] Please see Figure 1 , Figure 1 This is a schematic flowchart illustrating an embodiment of the sample dilution method of this application. The sample dilution method of this embodiment includes the following steps:

[0033] S11: Estimate at least one dilution ratio.

[0034] The dilution ratio, which is the ratio between the sample volume and the volume of solution obtained from dilution, can be expressed as: The sample volume and diluent volume are the volumes of the sample and diluent used, respectively.

[0035] The dilution ratios for each step of the dilution process have the following relationships:

[0036] γ=γ1*…*γ n ………………(1)

[0037] Where γ represents the target dilution ratio, γ1, ..., γ n These are the dilution ratios used in multiple dilution steps, where n is an integer greater than or equal to 2.

[0038] In this step, at least one dilution ratio can be estimated based on the number of dilutions and the target dilution ratio. For example, the dilution ratios of multiple dilution steps can be determined to be close or equal values, thereby determining the estimated value of at least one dilution ratio. Alternatively, minimizing the dilution error of the current dilution step can be used as the target to determine the estimated value of a dilution ratio. Those skilled in the art can also estimate at least one dilution ratio in other ways. For example, when a sample needs to be diluted twice, another dilution ratio can be determined by estimating one of the dilutions. When a sample needs to be diluted three or more times, the dilution ratio of a certain dilution process can be determined by estimating multiple dilutions. For example, in a three-dilution process, the appropriate dilution ratio for the first dilution process can be gradually determined by estimating the dilution ratios of the second and third dilution processes.

[0039] S12: Determine another dilution ratio based on the target dilution ratio of the sample and at least one dilution ratio.

[0040] The target dilution ratio is the dilution factor of the sample in the target solution. Another dilution ratio can be determined by equation (1).

[0041] S13: Determine whether the other dilution ratio meets the preset requirements.

[0042] After setting preset requirements, and calculating another dilution ratio in step S12, this step is used to determine whether it meets the conditions.

[0043] If the other dilution ratio meets the preset requirements, proceed to step S15; otherwise, proceed to step S14.

[0044] S14: Adjust at least one dilution ratio.

[0045] Adjust each dilution ratio data in at least one dilution ratio by a preset step size, and return to step S12 to recalculate another dilution ratio. The adjustment direction can be either increasing or decreasing, and the adjustment step size for each dilution ratio data can be the same or different.

[0046] S15: Determine at least one current dilution ratio and another dilution ratio as the dilution ratio used in one of the multiple dilution processes of the sample.

[0047] The above method is used to determine at least one dilution ratio and another dilution ratio, wherein the at least one dilution ratio is the dilution ratio of one or more dilutions in a multi-dilution process, and the other dilution ratio is the dilution ratio of the remaining dilution processes.

[0048] After obtaining at least one dilution ratio and another dilution ratio, calculate the sample volume and diluent volume for each dilution ratio to facilitate the dilution operation of the corresponding steps based on the sample volume and diluent volume.

[0049] When at least one dilution ratio includes multiple dilution ratios, after determining another dilution ratio, the dilution ratio data in at least one dilution ratio can be further optimized. Specifically, some dilution ratio data in at least one dilution ratio can be estimated to determine one of the dilution ratio data, and step-by-step optimization can be performed according to steps S11 to S15 to obtain each dilution ratio data.

[0050] In this way, when the target dilution ratio is small, the dilution process can be divided into multiple dilution steps to improve the sampling accuracy of the sampling needle, thereby reducing the dilution ratio error of the target solution. Moreover, the multiple dilution processes in this application only calculate two dilution ratio data, resulting in less data processing.

[0051] Optionally, before step S11, it is determined whether the target dilution ratio is less than the preset dilution ratio. If it is less, steps S11 to S15 are executed to calculate the dilution ratio multiple times. Otherwise, a single dilution is performed at the target dilution ratio without calculating the dilution ratio multiple times. Specifically, the smaller the target dilution ratio, the greater the difference between the amount of sample to be taken and the amount of diluent. Performing multiple dilutions can improve accuracy.

[0052] The other dilution ratio includes a first dilution ratio, and the at least one dilution ratio includes a second dilution ratio, wherein the first dilution ratio and the second dilution ratio are respectively the dilution ratios used in one of the two dilution processes of the sample.

[0053] That is, step S11 can be an estimated first dilution ratio, and step S12 is to determine the second dilution ratio based on the first dilution ratio and the target dilution ratio; or step S11 can be an estimated second dilution ratio, and step S12 is to determine the first dilution ratio based on the second dilution ratio and the target dilution ratio.

[0054] In one embodiment, the sample dilution is divided into two dilution steps: a first dilution and a second dilution. The first dilution uses a first dilution ratio, and the second dilution uses a second dilution ratio. The first dilution samples the first sample and the diluent, while the second dilution samples the solution obtained from the first dilution and the diluent, resulting in the target solution.

[0055] Please refer to the following: Figure 2 and Figure 3 , Figure 2 This is a schematic flowchart of another embodiment of the sample dilution method of this application. Figure 3 This is a flowchart illustrating the process of this embodiment.

[0056] The dilution steps in this embodiment include a first dilution and a second dilution. The first dilution uses ρa1μL of the first sample and ρb1μL of diluent to obtain a diluted solution. The second dilution uses a2μL of the second sample and b2μL of diluent to obtain the target solution. The second sample is obtained from the solution after the first dilution.

[0057] The volume of the solution obtained from the first dilution can be expressed as:

[0058] V1=ρa1+ρb1……………………(2)

[0059] Where ρ is the scaling factor.

[0060] The volume of the target solution obtained from the second dilution can be expressed as:

[0061] V2=a2+b2……………………(3)

[0062] The first dilution ratio can be expressed as:

[0063]

[0064] The second dilution ratio can be expressed as:

[0065]

[0066] The sample dilution method in this embodiment includes the following steps:

[0067] S21: Estimated second dilution ratio.

[0068] This step allows you to preset the dilution ratio as an estimate of the second dilution ratio.

[0069] For example, in one embodiment, 0.5 is used as the estimated value for the second dilution ratio. Specifically, when the second dilution ratio is 0.5, the volume of the second sample taken during dilution is equal to the volume of the diluent taken. For the second dilution step, there is no risk of exceeding the measurement accuracy and range of the sampling needle, resulting in high accuracy. Those skilled in the art can certainly preset other values ​​as the estimated value for the second dilution ratio.

[0070] S22: Determine the first dilution ratio based on the target dilution ratio and the second dilution ratio of the sample.

[0071] The first dilution ratio is determined using the following relationship:

[0072] γ=γ1·γ2……………………(6)

[0073] Wherein, γ is the target dilution ratio, γ1 is the first dilution ratio, and γ2 is the second dilution ratio. The target dilution ratio γ and the second dilution ratio γ2 are both known quantities. The first dilution ratio γ1 can be calculated by formula (6).

[0074] S23: Determine whether the first dilution ratio meets the preset requirements.

[0075] If the first dilution ratio γ1 meets the preset requirements, then proceed to step S25; otherwise, proceed to step S24 to adjust the second dilution ratio.

[0076] The preset requirements can be determined through the following steps S31 to S32.

[0077] S31: Obtain: the volume of the target solution, the range of solution volumes obtained in the first dilution process, and the ratio of the volume of the first sample used in the first dilution process to the volume of the second sample used in the second dilution process.

[0078] This step determines the volume of the target solution as V, where:

[0079] V=V2=a2+b2……………………(7)

[0080] The volume range of the solution obtained from the first dilution process is:

[0081] V min ≤V1≤V max ……………………(8)

[0082] The ratio of the volume of the first sample used in the first dilution process to the volume of the second sample used in the second dilution process is as follows:

[0083] ρa1=La2……………………(9)

[0084] S32: Determine the preset requirements using the volume of the target solution, the range of intermediate sample volumes, the first dilution ratio, the target dilution ratio, and the proportional relationship.

[0085] Use the following formula to determine the preset requirements:

[0086]

[0087] Among them, V min V represents the minimum range of solution volumes obtained from the first dilution. max γ1 represents the maximum range of solution volumes obtained from the first dilution, γ2 represents the target solution volume, γ1 represents the first dilution ratio, γ2 represents the second dilution ratio, and L represents the ratio of the volume of the first sample used in the first dilution process to the volume of the second sample used in the second dilution process.

[0088] If the first dilution ratio γ1 calculated in step S22 satisfies the condition of equation (10), then the first dilution ratio γ1 is considered to meet the preset requirements.

[0089] Among them, V min V max And V can be determined by those skilled in the art based on the volume of the dilution container used or the range of the sampling needle, etc.

[0090] Specifically, step S32 utilizes the volume V of the target solution and the intermediate sample volume range V. min ~V max The method for determining the preset requirements for the first dilution ratio γ1, the target dilution ratio γ, and the proportional relationship L is as follows:

[0091] Combining equations (2) and (4), we can conclude that:

[0092] ρa1=γ1·V1……………………(11)

[0093] Combining equations (5) and (7), we can conclude that:

[0094] a2=γ2·V……………………(12)

[0095] Combining equations (9), (11), and (12), we can derive...

[0096]

[0097] Combining equations (8) and (13), we can derive the presupposition conditions of equation (10).

[0098] Optionally, the ratio of the volume of the first sample used in the first dilution process to the volume of the second sample used in the second dilution process is 1:1, i.e., L = 1. In this case, the preset condition is:

[0099]

[0100] The advantage of setting the ratio of the volume of the original sample used in the first dilution process to the volume of the intermediate sample used in the second dilution process to 1:1 in this embodiment is that the sample volumes used in the two dilution processes are equal, which can minimize the sample addition error and improve the dilution accuracy.

[0101] S24: Adjust the second dilution ratio.

[0102] If the first dilution ratio does not meet the preset requirements, the second dilution ratio is adjusted with a preset adjustment step size. Optionally, the second dilution ratio is decreased with a preset adjustment step size of 0.1 until the first dilution ratio meets the preset requirements.

[0103] After adjusting the dilution ratio, this step returns to step S22 to redetermine the first dilution ratio until the first dilution ratio meets the preset requirements.

[0104] In another embodiment, step S24 may also involve adjusting the volume of the intermediate sample to be used in the second dilution. Specifically, the volume a2 of the intermediate sample to be used in the second dilution may be adjusted to an integer multiple of the sampling needle graduation value. Here, the graduation value is the measurable value between two adjacent graduation values ​​on the sampling needle or sampling syringe.

[0105] S25: Determine the current first dilution ratio as the dilution ratio used in the first dilution process, and determine the current second dilution ratio as the dilution ratio used in the second dilution process.

[0106] After determining the first dilution ratio and the second dilution ratio, the amount of the first sample ρa1 and the amount of diluent ρb1 for the first dilution can be determined by combining equations (9) and (12), and the amount of the second sample a2 and the amount of diluent b2 for the second dilution can be determined by combining equations (5) and (12).

[0107] Using the method described above, this embodiment can calculate a suitable first dilution ratio and a second dilution ratio. These are used for the first and second dilutions, respectively, to improve the accuracy of each dilution step.

[0108] In another embodiment, the dilution ratio data for each dilution step in a dilution process with more than two dilution steps can also be determined using the above method. The relationship between the dilution ratio data for multiple dilution processes is: γ = (α)·(β)Where α and β are both integers greater than or equal to 1, and α+β is the number of dilutions to be performed, γ represents the target dilution ratio, and γ′ and γ″ are the dilution ratios for each dilution process. In some dilution processes, the same dilution ratio data is used. If either α or β is greater than 1 or both are greater than 1, the dilution ratio data for each step can be determined according to the above steps S21 to S25. Specifically, in the dilution step with dilution ratio γ′, the volume of the diluted solution to be obtained in each dilution step is determined to be equal and to satisfy the volume range of equation (8); in the dilution step with dilution ratio γ″, the volume of the diluted solution to be obtained in each dilution step is determined to be equal and to be the preset value V. γ′ and γ″ are respectively used as the first dilution ratio and the second dilution ratio in steps S21 to S25. In this way, γ′ and γ″ can be determined using steps S21 to S25. For example, in a three-stage dilution process, the dilution ratios of the first and second dilutions can be made equal, with a dilution ratio of γ′, and the dilution ratio of the third dilution is γ″. This can be achieved by ensuring that the volumes of the solutions obtained from the first and second dilutions are equal and satisfy the volume range of equation (8), and that the volume of the diluted solution to be obtained from the third dilution is a preset value V. γ′ and γ″ are then used as the first and second dilution ratios in steps S21 to S25, respectively. The dilution ratio γ′ of the first and second dilutions is determined using the method in steps S21 to S25. And the dilution ratio γ″ for the third dilution. In the process of three dilutions, the dilution ratio of the first dilution can be γ′, and the dilution ratio of the second and third dilutions can be γ″. The volume of the solution obtained from the first dilution is determined to satisfy the volume range of equation (8), and the volume of the solution to be obtained from the third dilution is the preset value V. Similarly, γ′ and γ″ can be used as the first dilution ratio and the second dilution ratio in steps S21 to S25, respectively. The dilution ratio γ′ of the first dilution and the dilution ratio γ″ of the second and third dilutions are determined by the method of steps S21 to S25.

[0109] Alternatively, when more than three dilutions are required, for example, in n dilution processes, the dilution ratios for each dilution step are γ1, γ2, ..., γ... n , where γ=γ1*γ2*...*γ n γ is the target dilution ratio. One of the dilution ratio values ​​can be determined by using partial dilution ratio values. For example, when n=3, γ2 and γ3 can be determined first to obtain γ1. Depending on whether γ1 meets the preset requirements, γ2 and γ3 can be adjusted until γ1 meets the preset requirements. γ2 and γ3 can also be optimized after γ1 is determined. In this way, multiple dilution ratio data can be obtained step by step to meet the requirements of multiple dilution times.

[0110] Please see Figure 5 , Figure 5This is a schematic diagram of an embodiment of the sample dilution device of this application. The sample dilution device 10 is provided with a liquid addition position 100, a transfer position 200, and a liquid addition mechanism 30. The liquid addition position 100 is used to load a first dilution cup. The liquid addition mechanism 30 is located above the liquid addition position 100 and is used to draw diluent and sample solution, and add the drawn diluent and sample solution to the first dilution cup located at the liquid addition position to form a primary diluted sample solution. The transfer position 200 is located below the liquid addition mechanism 30 and is used to receive the first dilution cup containing the primary diluted sample solution. The liquid addition mechanism 30 is also used to draw diluent and primary diluted sample solution and add them to a second dilution cup to form a secondary diluted sample solution.

[0111] The first-dilution sample solution is the diluted sample solution obtained by diluting the sample for the first time, and the second-dilution sample solution is the diluted sample solution obtained by diluting the sample (the sample solution obtained after the first dilution) for the second time. The sample dilution device 10 can also perform more than two dilution operations, such as three-dilution, four-dilution, or even five-dilution operations.

[0112] The liquid dispensing mechanism 30 can be a structure such as a sampling needle that can quantitatively transfer liquids. The amount of sample liquid and diluent drawn by the liquid dispensing mechanism each time is in accordance with the amount of sample liquid and diluent required for each dilution step.

[0113] Specifically, the liquid filling level 100 is a set position, which is a fixed position in space. At this set position, the liquid filling mechanism 30 can be set to discharge the sample liquid and diluent above the set position, so that the sample liquid and diluent are added to the dilution cup located at the liquid filling level 100.

[0114] The transfer station 200 is another designated location. In a two-stage dilution process, it is used to hold a dilution cup containing the first-stage diluted sample solution. In three or more dilution processes, it can be used to hold a dilution cup containing an intermediate-stage diluted sample solution, so that in the next dilution step, the dispensing mechanism 30 can draw the intermediate-stage diluted sample solution from the transfer station 200 for the next dilution. The intermediate-stage diluted sample solution is the diluted sample solution obtained in the dilution step before the final dilution. For example, when performing a three-stage dilution, in terms of the operational sequence, the transfer station 200 is used sequentially to place the dilution cup containing the first-stage diluted sample solution and the dilution cup containing the second-stage diluted sample solution. After obtaining the third-stage diluted sample solution, which is to be used for testing, the transfer station 200 does not transfer it.

[0115] The intermediate transfer position 200 can be set at a position adjacent to the liquid addition position 100, so that the liquid addition mechanism 30 can move to the liquid addition position 100 after drawing the intermediate diluted sample solution at the intermediate transfer position for the next dilution.

[0116] In one embodiment, the sample dilution device 10 further includes a rotating mechanism 20, which includes a fixed base 21 and a rotating base 22. The rotating base 22 is disposed inside the fixed base 21 and can rotate relative to the fixed base 21. The outer edge of the rotating base 22 is provided with a plurality of dilution cup holes 220. When the dilution cup holes 220 rotate with the rotating base 22, they will pass through the liquid filling position 100. In this way, the dilution cup loaded in each dilution cup hole 220 can be driven to the liquid filling position 100 in sequence with the rotation of the rotating base 22, and can receive the liquid drawn by the liquid filling mechanism 30 at the liquid filling position 100, and perform each dilution step in sequence.

[0117] In this embodiment, when a dilution cup is located at the liquid filling level 100, the cup opening is protruding to facilitate placement, transfer, or mixing. In one feasible embodiment, a pad is provided at the bottom of the fixing base 21 corresponding to the liquid filling level 100. The pad has two inclined surfaces along the circumferential direction of the rotating base 22. Regardless of whether the rotating base 22 rotates clockwise or counterclockwise, when a dilution cup is rotated to the liquid filling level 100, the dilution cup is gradually raised, causing its opening to protrude beyond the dilution cup opening for easy placement and removal. Unless otherwise specified, any dilution cup mentioned in this document can be the first dilution cup, the second dilution cup, or any dilution cup used in any dilution process.

[0118] For example, in a secondary dilution process, the rotating seat 22 rotates to move the first dilution cup located in one of the dilution cup holes 220 to the liquid addition position 100. The liquid addition mechanism 30 adds the sample solution and diluent to the first dilution cup to obtain a primary dilution. The first dilution cup is then transferred to the intermediate position 200. The rotating seat 22 rotates to move the second dilution cup located in one of the dilution cup holes 220 to the liquid addition position 100. The liquid addition mechanism 30 draws the primary diluted sample solution from the first dilution cup located at the intermediate position 200 and draws the diluent from the container containing the diluent. The drawn primary diluted sample solution and diluent are added to the second dilution cup located at the liquid addition position 100 to obtain a secondary dilution. To perform multiple dilutions, after the dispensing mechanism 30 has drawn the diluted sample solution once, the first dilution cup is discarded, freeing up the transfer position 200. After obtaining the second dilution solution, the transfer position 200 of the second dilution cup and the rotating seat 22 are rotated, moving the third dilution cup located in one of the dilution cup holes 220 to the dispensing position 100. The dispensing mechanism 30 draws the diluted sample solution once from the second dilution cup located in the transfer position 200 and draws the dilution solution. The drawn diluted sample solution and the dilution solution are added to the third dilution cup located in the dispensing position 100 to obtain the third dilution solution. To perform multiple dilutions, the operation sequence described above is followed to control the coordination of each component, which will not be elaborated further.

[0119] In one feasible embodiment, the rotating mechanism 20 includes a motor 23, which is fixedly mounted on the fixed base 21. A rotating base 22 is connected to the output end of the motor 23. The rotation of the motor 23 drives the rotating base 22 to rotate clockwise or counterclockwise, thereby sequentially rotating the dilution cup holes 220 to the liquid filling position 100, enabling automatic loading of the dilution cup at the liquid filling position 100. The dilution cup holes 220 are evenly distributed along the edge of the rotating base 22. Controlling the motor 23 to rotate a preset angle each time ensures that the dilution cup holes 220 are sequentially positioned at the liquid filling position 100. For example… Figure 1 The rotating base 22 shown has four dilution cup holes 220, which are evenly distributed along the edge of the rotating base 22. Each 90-degree rotation of the rotating base 22 positions each dilution cup hole 220 sequentially at the liquid filling level 100, thus enabling the dilution cups loaded in the dilution cup holes 220 to be sequentially transferred to the liquid filling level 100. Of course, the number of dilution cup holes can be set according to actual needs, and therefore is not specifically limited.

[0120] The rotating mechanism 20 also includes a dilution cup channel 24, which has a receiving groove 240 for holding at least one dilution cup. One end of the dilution cup channel 24 is connected to a cup-adding mechanism (not shown) for adding dilution cups to the receiving groove 240. The other end of the dilution cup channel 24 is connected to a rotating seat. When the receiving groove 240 is connected to at least one dilution cup hole 220, the dilution cups in the receiving groove 240 that are close to the dilution cup hole 220 are transferred to the dilution cup hole 220 for automatic filling. A first detection optocoupler 25 is provided inside the receiving groove 240 to detect the dilution cups in the receiving groove 240 and control the number of dilution cups in the receiving groove 240.

[0121] In a feasible embodiment, a second detection optocoupler 26 is provided on the side of the fixed base 21 at the position corresponding to the liquid level 100. The second detection optocoupler 26 is used to detect the position of the dilution cup at the liquid level 100 so as to control operations such as liquid addition or dilution cup transfer.

[0122] In one embodiment, the dilution cup orifice 220 passes sequentially through the liquid filling position 100 and the intermediate position 200 as the rotating seat 22 rotates. That is, the intermediate position 200 is set as a fixed position in space. When the rotating seat 22 rotates, the dilution cup orifice 220 can be located at the intermediate position 200 in sequence. Moreover, the dilution cup orifice 220 is first located at the liquid filling position 100 and then at the intermediate position 200.

[0123] In this embodiment, the dilution cup hole 220 can be located in the intermediate position 200 as the rotating seat 22 rotates. That is, the dilution cup containing the intermediate dilution sample solution is carried by the dilution cup hole 220, without the need to open a separate station as an intermediate station. This makes the internal structure of the dilution device more compact and improves the space utilization rate.

[0124] For a specific dilution procedure, please refer to Figure 10 , Figure 10 This is a schematic diagram of an embodiment of the rotary seat of this application. A first dilution cup 51 and a second dilution cup 52 are respectively located in one of the dilution cup holes 220. As the rotary seat 22 rotates, the first dilution cup 51 can be moved to the liquid filling position 100 for the first dilution operation. After the rotary seat 22 rotates once, the second dilution cup 52 is transferred to the liquid filling position 100. At this time, the first dilution cup 51 is located at the intermediate position 200, where the second dilution operation is performed. The first dilution cup 51 can be moved to the intermediate position 200 by the rotation of the rotary seat 22, or it can be clamped and transferred to the intermediate position 200.

[0125] In a feasible embodiment, a pad is provided at the bottom of the fixed base 21 corresponding to the position of the intermediate position 200. The pad has two inclined surfaces along the circumferential direction of the rotation of the rotating base 22. Regardless of whether the rotating base 22 rotates counterclockwise or clockwise, when a dilution cup is driven to rotate to the intermediate position 200, the dilution cup can be gradually raised so that the cup mouth protrudes from the dilution cup hole, making it easy to be clamped; or when a dilution cup is placed at the intermediate position 200, the cup mouth of the dilution cup protrudes from the dilution cup hole, making it easy to pick up and put down the dilution cup.

[0126] Please continue reading for more details. Figure 1 A third detection optocoupler 27 can be installed on the side of the fixed base 21 corresponding to the position of the transfer position 200. The third detection optocoupler 27 is used to detect the presence or absence of the dilution cup in the transfer position 200, so as to control the liquid dispensing mechanism 30 to perform liquid dispensing operation from the dilution cup in the transfer position 200.

[0127] The sample dilution device 10 may further include a transfer assembly 40, which is positioned above the rotating mechanism 20 and is used to transfer the dilution cup. Please refer to [link to relevant documentation]. Figure 7 , Figure 7 This is a schematic diagram of the structure of an embodiment of the transfer component of this application.

[0128] The transfer assembly 40 includes a motion mechanism 60 and a mixing mechanism 70. The motion mechanism 60 enables the mixing mechanism 70 to grip and transfer the first dilution cup at the liquid level 100. The mixing mechanism 70 mixes the first dilution cup during the gripping and transfer process. In three or more dilution processes, the transfer assembly 40 can also operate on other dilution cups.

[0129] In another feasible embodiment, instead of setting the transfer station at the position corresponding to the dilution cup orifice 220, the mixing mechanism 70 can be used as the transfer station. Specifically, in the secondary dilution process, the first dilution cup is placed in the mixing mechanism 70. During the secondary dilution operation, the liquid addition mechanism 30 directly draws the diluted sample solution once from the mixing mechanism 70. In this way, while the mixing mechanism 70 is mixing the first dilution cup, the rotating mechanism 20 can be controlled to move the second dilution cup to the liquid addition position 100. After the first dilution cup is mixed, the liquid addition mechanism 30 can directly draw the diluted sample solution once from the mixing mechanism 70. There is no need to set up a separate station as a transfer station, which can effectively save space. There is also no need to transfer the first dilution cup to other stations for liquid retrieval, which can save operation time and improve dilution efficiency.

[0130] Please see Figure 8 The dashed line in the figure represents the movement path of the transfer component 40. The intermediate transfer position 200 can be set at a certain position on the movement path of the transfer component 40 so that when the dilution cup is transferred, the liquid addition mechanism 30 can draw intermediate diluted sample solution from the dilution cup located in the transfer component 40.

[0131] The transfer component 40 can be a three-axis motion mechanism, capable of moving relative to the liquid level 100 in the X and / or Y and / or Z directions. The intermediate transfer position 200 and the liquid level 100 are arranged in the same straight line in the X or Y direction. Figure 8 The X and Y directions are for illustrative purposes only and do not imply that they are on the same plane. The Z-axis movement allows the transfer component 40 to move vertically for picking up and placing the dilution cup.

[0132] For a specific dilution procedure, please refer to [link / reference needed]. Figure 8The solid line represents the movement path of the liquid adding mechanism 30, and the dashed line represents the movement path of the transfer component 40. To clearly show the movement paths of the liquid adding mechanism 30 and the transfer component 40, the dashed and solid lines are offset in the diagram. In actual operation, the paths represented by the solid and dashed lines can be set to coincide. In the dilution process, the first dilution cup is loaded at the liquid adding position 100. The liquid adding mechanism 30 moves along path AB, sequentially passing through the container 11 containing the diluent and the container 12 containing the sample solution, drawing the required amounts of diluent and sample solution respectively. Then, it moves along path BC, and upon reaching the liquid adding position 100, it adds the drawn liquid to the first dilution cup. The transfer component 40 moves the first dilution cup out of the liquid adding position 100, mixes it, and moves along path CE to the intermediate transfer position 200. It then controls the rotating seat 22 to rotate, transferring the second dilution cup to the liquid adding position 100. The liquid adding mechanism 30 then adds the liquid... After being added to the first dilution cup, the component moves along path CA, cleaning itself in the process. It then moves along path AB, passing through container 11 containing the diluent, where a quantitative amount of diluent is drawn. Next, it moves along path BC, drawing a quantitative amount of diluted sample solution from the first dilution cup at transfer station 200. At liquid addition station 100, the drawn liquid is added to the second dilution cup, resulting in a second-diluted sample solution. After the liquid addition mechanism 30 draws a diluted sample solution from the first dilution cup at transfer station 200, the transfer component 40 can move along path EDC, discarding the first dilution cup in the process. If a second dilution is performed, the second dilution cup is mixed and transferred to the detection area for testing. If further dilution steps are required, the components continue operating according to the aforementioned coordination method until the target diluted solution is obtained. In this way, intermediate diluted sample solution can be aspirated during the mixing, transport and disposal of the dilution cup, without having to transport the dilution cup to a specific transfer station. The various components of the sample dilution device 10 can cooperate efficiently, improving dilution efficiency. On the other hand, there is no need to set up a separate transfer station, improving space utilization.

[0133] The path settings in this embodiment are for illustrative purposes only, and the movement direction and transport path of each component may not be the same. Figure 8 As described, there are other ways besides the one described. The direction of movement of the transfer component 40 relative to the liquid level 100 is not limited to the X and Y directions. There are many ways, which will not be listed here.

[0134] Please refer to the following: Figure 7 and Figure 9 , Figure 9 This is an exploded view of an embodiment of the transfer component of this application.

[0135] The transfer assembly 40 includes a motion mechanism 60 and a mixing mechanism 70. The mixing mechanism 70 includes a limiting block 71, a gripper slider 72, a gripper assembly 73, and a rotating shaft 74. The limiting block 71 is provided with a limiting groove 710. One end of the gripper slider 72 is clearance-fitted with the limiting groove 710, and the other end is fixedly connected to the gripper assembly 73. The gripper assembly 73 is used to grip the dilution cup. The rotating shaft 74 is clearance-fitted with the gripper slider 72 so that the rotation of the rotating shaft 74 drives the gripper slider 72 and the gripper assembly 73 to shake, thereby realizing the mixing operation of the sample in the dilution cup.

[0136] The gripper slider 72 has a first limiting hole 721 and a second limiting hole 722. The first limiting hole 721 is used to engage with the aforementioned rotating shaft 74, so that when the rotating shaft 74 rotates, it causes the gripper slider 72 to oscillate within the limiting groove 710. The motion mechanism 60 is disposed on one side of the limiting block 71. The motion mechanism 60 can drive the limiting block 71 to drive the gripper slider 72 to move axially relative to the rotating shaft 74, and then drive the gripper assembly 73 to move axially relative to the rotating shaft 74 through the gripper slider 72.

[0137] The mixing mechanism 70 further includes a limiting pin 711 disposed within the limiting block 71 and an eccentric driven wheel 712 sleeved on the limiting pin 711, the eccentric driven wheel 712 being sleeved with the second limiting hole 722. The secondary axis of the rotating shaft 74 has a first eccentricity distance with respect to the main axis, and the axis of the limiting pin 711 has a second eccentricity distance with respect to the axis of the eccentric driven wheel 712. The first eccentricity distance is less than or equal to the second eccentricity distance. In this embodiment, an eccentric driven wheel 712 is sleeved on the gripper slider 72 to rotate in coordination with the rotating shaft 74, thereby improving the responsiveness of the gripper slider 72.

[0138] In a feasible embodiment, the mixing mechanism 70 further includes a base frame 75, a top frame 76, and a guide rod 77 connecting the base frame 75 and the top frame 76. A limiting block 71 and a gripper slider 72 are movably disposed between the base frame 75 and the top frame 76 based on the guide rod 77 and the rotating shaft 74. Specifically, through holes 1100 are respectively opened on both sides of the limiting block 71, and the guide rod 77 passes through the through holes 1100. When the limiting block 71 and the gripper slider 72 are driven by an external force, they can move axially within the area between the base frame 75 and the top frame 76. A bearing 82 that transitionally engages with the rotating shaft 74 is also fixedly mounted on the base frame 75. The end of the rotating shaft 74 near the base frame 75 engages with the bearing 82 to rotate.

[0139] Please see Figure 10 , Figure 10 For this application Figure 9A schematic diagram of one embodiment of the rotating shaft. The rotating shaft 74 includes a main shaft section 741, a transition section 742, and a secondary shaft section 743 connected in sequence. The main shaft section 741 and the secondary shaft section 743 have the same outer diameter, while the outer diameter of the transition section 742 is smaller than that of the main shaft section 741 and the secondary shaft section 743. When the rotating shaft 74 is assembled with the gripper slider 72, there is a certain gap between the rotating shaft 74 and the gripper slider 72 at the engagement position. The gripper slider 72 moves axially relative to the rotating shaft 74, sliding from the transition section 742 to the secondary shaft section 743, which can realize the mixing operation of the dilution cup, thereby avoiding jamming of the mixing device due to the influence of the outer diameter of the transition section 742 during the switching of operating states. The transition section 742 is frustum-shaped, and the cross-sectional area of ​​the side of the transition section 742 connected to the main shaft section 741 and the secondary shaft section 743 is the same as the cross-sectional area of ​​the main shaft section 741 and the secondary shaft section 743.

[0140] Furthermore, the axis of the secondary shaft section 743 is not on the same straight line as the axis of the main shaft section 741; that is, the secondary axis of the secondary shaft section 743 is eccentrically positioned relative to the main axis of the main shaft section 741. Because the rotating shaft 74 and the gripper slider 72 are fitted together with a gap, when the rotating shaft 74 is driven by an external force, it rotates based on the main axis. When the gripper slider 72 is located in the secondary shaft section 743, the secondary shaft section 743 causes the gripper slider 72 to sway within the limiting groove 710, which in turn causes the gripper assembly 73 to sway, thereby mixing the dilution cup. When the gripper slider 72 is located in the main shaft section 741, it can grip the dilution cup, or release or discard the gripped dilution cup.

[0141] In one feasible embodiment, please continue to refer to Figure 9 The limiting block 71 includes a bottom block 714 and a surface block 715, and the limiting groove 710 is located between the bottom block 714 and the surface block 715.

[0142] In a feasible embodiment, the mixing mechanism 70 further includes an optical coupler 78. A baffle 713 corresponding to the optical coupler 78 is provided on the limiting block 71. The optical coupler 78 is used to obtain the positioning state of the baffle 713 to correspondingly determine the positioning state of the gripper slider 72 relative to the secondary shaft section 743. Specifically, when the optical coupler 78 detects the baffle 713, the gripper slider 72 is in the secondary shaft section 743; otherwise, the gripper slider 72 is not in the secondary shaft section 743.

[0143] In one feasible embodiment, the mixing mechanism 70 further includes a rotary motor 79, the output end of which is connected to the secondary shaft section 743. (See also...) Figure 6The free end of the secondary shaft section 743 is provided with a mating hole 744 concentric with the main shaft section 741. The mating hole 744 is used to mate with the output end of the rotary motor 79, so that the output end of the rotary motor 79 corresponds to the mating holes 744 on the main shaft section 741 and the secondary shaft section 743. When the rotary motor 79 is working, the rotating shaft 74 responds to the rotation of the rotary motor 79 and rotates around the main shaft center. This, in turn, drives the gripper slider 72 and the gripper assembly 73 to shake through the secondary shaft section 743, thereby mixing the dilution cup held in the grip. The rotary motor 79 can be directly fixedly installed, or it can be connected to the rotating shaft 74 through a motor mounting base 83 provided between the rotary motor 79 and the limit block 71.

[0144] In one feasible embodiment, the gripper assembly 73 includes a gripper capable of elastically opening and closing. The gripper includes two opposing grippers 731 and 732, which work together to hold the dilution cup. The gripper assembly 73 further includes a limiting member 733, a guide member 734, and an elastic member 735. The second grippers 731 and 732 pass through the guide member 734 and slide along the guide member 734 to bring the second grippers 731 and 732 closer together or further apart to grip or release the dilution cup. The elastic member 735 is disposed between the second grippers 731 and 732 to connect the second grippers 731 and 732. The elastic member 735 can stretch or contract under external force, thereby clamping the second grippers 731 and 732 to hold the dilution cup in a clamped state. The limiting member 733 is disposed on the outside of the second gripper 731 and the second gripper 732 to limit the sliding space of the second gripper 731 and the second gripper 732 on the guide member 734 and prevent the second gripper 731 and the second gripper 732 from falling off. In a feasible embodiment, the elastic member 735 is a spring.

[0145] The motion mechanism 60 includes a lifting motor 61, a driving wheel 62 connected to the output end of the lifting motor 61, a driven wheel 63 spaced apart from the driving wheel, a synchronous belt 64 sleeved on the driving wheel 62 and the driven wheel 63, and a linkage 65 connecting the synchronous belt 64 to the limiting block 71. The lifting motor 61 drives the synchronous belt 64 to rotate in both directions, and then the linkage 65 drives the limiting block 71 to move axially relative to the guide rod 77 and the rotating shaft 74. The linkage 65 presses the synchronous belt 64 partially onto the limiting block 71, so that the limiting block 71 moves synchronously with the synchronous belt 64, and drives the gripper slider 72 to move axially along the rotating shaft 74, so that the gripper slider 72 can be in the main shaft section 741 or the secondary shaft section 743.

[0146] In one embodiment, the linkage 65 includes a first pressing and fixing member 651, which directly presses and connects a portion of the timing belt 64 to the limiting block 71.

[0147] In another embodiment, the linkage 65 includes a first pressing and fixing member 651 and a second pressing and fixing member 652. The first pressing and fixing member 651 has a groove on its side near the second pressing and fixing member 652. The portion of the timing belt 64 used to connect to the limiting block 71 is accommodated within the groove. The timing belt 64 is partially fixed within the groove by the first pressing and fixing member 651 and the second pressing and fixing member 652, and thus connected to the limiting block 71. The linkage 65 can be fixedly connected to the timing belt 64 and the limiting block 71 by bolts or screws, or by a snap-fit ​​connection, as long as the linkage 65 can drive the movement of the limiting block 71.

[0148] Furthermore, the mixing mechanism 70 may include a protective member 80, which is disposed on the top frame 76 and corresponding to the exposed portion of the rotary motor 79, for protecting the rotary motor 79 and other components of the mixing mechanism 70.

[0149] Furthermore, the mixing mechanism 70 may include a dilution cup detection optocoupler 81, which is disposed on the base frame 75 at a position opposite to the direction of the gripper, and is used to detect the gripper or the dilution cup held by the gripper to determine whether the gripper slider 72 is located in the spindle section 741.

[0150] The transfer component 40 of this embodiment has both transfer and mixing functions, which can significantly improve the efficiency of transfer and mixing during the dilution process, especially during multiple dilution operations.

[0151] In another embodiment, the transfer position may not be located at the position corresponding to the dilution cup orifice 220, nor may it be located in the mixing mechanism 70. For example, the rotating mechanism 20 may be embedded in the cup carrier platform (not shown in the figure). The cup carrier platform may be provided with other working areas or a station for placing dilution cups, so that the dilution device can operate in conjunction with other working areas. In this embodiment, a transfer position (not shown in the figure) may be provided on the cup carrier platform. When dilution cup transfer is required, the dilution cup is transferred to the transfer position on the cup carrier platform. When the liquid dispensing mechanism 30 needs to perform a liquid dispensing operation, it can be driven to dispensing liquid at the transfer position located on the cup carrier platform.

[0152] The intermediate position is a countersunk hole recessed into the surface of the cup-carrying platform. The countersunk hole can be round or square. A round hole can be used to carry an optical cup, while a square hole can be used to carry a magnetic bead cup. The depth of the countersunk hole is less than the height of the first or second dilution cup, allowing the rim of the dilution cup located in the countersunk hole to protrude from the surface of the cup-carrying platform for easy placement and removal of the dilution cup.

[0153] Please see Figure 11 , Figure 11This is a schematic diagram of another embodiment of the rotating mechanism of this application. The rotating mechanism 20 of this embodiment includes a fixed base 21 and a rotating base 22. The rotating base 22 is provided with multiple dilution cup holes 220. A liquid filling position 100 is provided corresponding to one of the dilution cup holes 220. The dilution cup hole 220 is used to load dilution cups. The rotating base 22 can rotate clockwise or counterclockwise to sequentially move the dilution cups located in the dilution cup holes 220 to the liquid filling position 100. In this embodiment, a transfer position 300 is provided in the fixed base 21. This transfer position 300 is a recessed countersunk hole provided on the surface of the fixed base 21. Similar to the previous embodiment, the transfer position 300 is used to load dilution cups containing intermediate dilution sample solution, so that in the next dilution step, the liquid adding mechanism 30 can draw intermediate dilution sample solution from the transfer position 300 for the next dilution step.

[0154] The countersunk hole of the intermediate position 300 can be a square hole or a round hole. The round hole can be used to support the optical cup, and the square hole can be used to support the magnetic bead cup.

[0155] Alternatively, the mounting base 21 may have two transfer positions, both of which are recessed countersunk holes. One transfer position has a square countersunk hole for transferring the magnetic bead cup, while the other transfer position has a round countersunk hole for transferring the optical cup. The structure of this embodiment allows for the selection of transfer positions according to actual needs. For example, please refer to [further details omitted]. Figure 11 The two intermediate transfer positions are transfer position 300 and transfer position 400. The countersunk hole of transfer position 300 is a round hole, and the countersunk hole of transfer position 400 is a square hole. When an optical cup is needed for dilution operation, transfer position 300 is selected for dilution cup transfer. When a magnetic bead cup is needed for dilution operation, transfer position 400 is selected for dilution cup transfer. It is suitable for dilution operation of various detection items and can improve the utilization rate of sample dilution device 10.

[0156] The depth of the countersunk holes at the two intermediate positions is less than the height of the first or second dilution cup. In short, the cup mouth of the dilution cup located at the intermediate position 300 or 400 protrudes from the surface of the fixing base 21 for easy placement and removal.

[0157] In all the embodiments described above, the transfer component 40 can be used to perform mixing and transfer operations on the dilution cup. For example, in one embodiment, the dilution process for a sample is as follows: When the first dilution cup is driven to the liquid filling position 100 by the rotating mechanism 20, the liquid filling mechanism 30 moves above the first dilution cup at the liquid filling position 100 and adds the absorbed diluent and sample solution to the first dilution cup, resulting in a first-diluted sample solution in the first dilution cup. Subsequently, the transfer component 40 moves above the first dilution cup at the liquid filling position 100 and clamps the first dilution cup by the mixing mechanism 70 provided on the transfer component 40. The motion mechanism 60 drives the gripper slider 72 to move to the secondary shaft section 743 of the rotating shaft 74. After the movement, the bottom of the first dilution cup can completely avoid the height of the dilution cup at the liquid filling position 100. The rotating mechanism 20 is controlled to move, driving the second dilution cup to the liquid filling position 100. The rotating motor 79 drives the rotating shaft 74 to rotate, thereby mixing the first-diluted sample solution in the first dilution cup, completing one dilution. The first dilution cup is then moved to the subsequent detection position for detection. If a secondary dilution is required, the first dilution cup is moved to the transfer position, which can be any of the transfer positions described in the above embodiments. At this time, the second dilution cup is located at the liquid addition position 100. The liquid addition mechanism 30 is driven to move to the transfer position, and the diluted sample solution is drawn once from the first dilution cup. The liquid addition mechanism 30 also draws diluted solution from the diluted solution container and moves to the liquid addition position 100. The liquid addition mechanism 30 adds the drawn liquid to the second dilution cup located at the liquid addition position 100, and a secondary sample dilution is obtained in the second dilution cup. After the liquid addition mechanism 30 draws diluted sample solution once from the first dilution cup, the transfer component 40 is driven to transfer the first dilution cup to the cup dropping area for cup dropping operation. Then it moves to the liquid addition position 100, takes out the second dilution cup located at the liquid addition position 100, and performs the same mixing operation as the first dilution cup described above on the second dilution cup. The second dilution is mixed, and the rotating mechanism 20 is controlled to move to move the subsequently added dilution cup to the liquid addition position 100. After the second dilution cup is mixed, it is moved to the subsequent detection position for detection. If there is a need for multiple dilutions (three or more), the process steps for the first and second dilutions described above can be followed to drive each component to complete the process. All components work closely together in the entire dilution process to achieve highly efficient multiple dilutions.

[0158] The use of a first dilution cup and a second dilution cup in the description of the operation or effect of various components in the embodiments of this application does not imply a limitation on the use of each component in the device. The first dilution cup and the second dilution cup may also be other dilution cups.

[0159] Please see Figure 12 , Figure 12This is a schematic block diagram of the circuit structure of an embodiment of the sample analyzer of this application. The sample analyzer 500 includes a sample detection device 501 and a sample dilution device 502. The sample dilution device 502 can be any of the sample dilution devices provided in the above embodiments of this application. During the sample analysis process, the sample dilution device 502 prepares a sample to be tested that meets the detection requirements by dilution at least twice. The preparation method can refer to the dilution operation flow of the above embodiments of the sample dilution device, and will not be repeated here. The sample detection device 501 detects the sample to be tested.

[0160] The sample dilution device 502 can be applied to any sample analyzer that requires sample dilution, such as a hematology analyzer, bioanalytical analyzer, and coagulation analyzer. When the sample dilution device 502 is applied to a coagulation analyzer, the dilution cup can be a sample cup used for optical detection. When the sample analyzer can use optical and / or magnetic bead methods for detection, the intermediate dilution cup can be either an optical or magnetic bead cup. To save costs, when using magnetic bead methods, optical cups can be used before obtaining the target diluent, and the obtained target diluent is added to the magnetic bead cup for subsequent detection.

[0161] Please see Figure 13 This application also provides a method for detecting a sample. Taking a two-dilution sample as an example, the method includes the following steps:

[0162] S101: Determine the first dilution ratio and the second dilution ratio.

[0163] The first dilution ratio and the second dilution ratio are determined according to the steps of each embodiment of the sample dilution method.

[0164] S102: Based on the first dilution ratio, absorb the diluent and sample solution, and spit the absorbed liquid into the first dilution cup to obtain the first solution.

[0165] In this process, after determining the first dilution ratio and the second dilution ratio according to step S25, the amount of the first sample ρa1 and the amount of diluent ρb1 for the first dilution are determined, and the amount of the second sample a2 and the amount of diluent b2 for the second dilution are determined.

[0166] This step involves drawing up the sample solution and diluent according to the first sample volume ρa1 and the diluent volume ρb1, respectively. This first dilution cup is the same as the first dilution cup described in the above embodiments.

[0167] S103: Based on the second dilution ratio, absorb the diluent and the first solution, and then spit the absorbed liquid into the second dilution cup to obtain the second solution.

[0168] In this step, the first solution and the diluent can be drawn up according to the second sample volume a2 and the diluent volume b2. This second dilution cup is the second dilution cup described in the above embodiments.

[0169] S104: Detect the second solution.

[0170] In this embodiment, the second solution obtained by dilution in steps S102 to S103 is the solution used for detection. The dilution ratio of the sample in the second solution is the target dilution ratio. By using each step of the sample dilution method to determine the dilution ratio of each dilution step, errors caused by the sampling accuracy of the sampling needle can be reduced, making the dilution of the sample more accurate, and the detection results of the diluted sample are also more accurate.

[0171] Please see Figure 14 Step S102 may include:

[0172] S1021: Control the dispensing mechanism to draw up the diluent and sample solution based on the volume of diluent and sample solution determined by the first dilution ratio.

[0173] The dispensing mechanism can first move to the container containing the diluent, draw up the diluent, and then move to the container containing the sample solution to draw up the sample solution; or it can move to the container containing the sample solution first, draw up the sample solution, and then move to the container containing the diluent to draw up the diluent. The order is determined by the actual movement path of the dispensing mechanism and is not limited here.

[0174] S1022: Control the liquid dispensing mechanism to move to the liquid dispensing position, and dispense the aspirated diluent and sample solution into the first dilution cup located at the liquid dispensing position to obtain the first solution.

[0175] After this step, the liquid dispensing mechanism can be controlled to move to an area where cleaning can be performed to prepare for the next liquid dispensing.

[0176] S1023: Control the transfer component to transfer the first dilution cup to the intermediate transfer position.

[0177] The controllable transfer component can remove the first dilution cup, mix the first dilution cup evenly, and then move it to the intermediate transfer position; or, the controllable transfer component can mix the first dilution cup evenly during the path of transferring the first dilution cup to the intermediate transfer position.

[0178] Taking the transfer component 40 in the above embodiment as an example, controlling the transfer component to remove the first dilution cup may include steps a to b:

[0179] a: Control the transfer component to move to the liquid collection position;

[0180] b: Control the motion mechanism 60 to work, so that the gripper slider 72 moves to the main shaft section 741 and grips the first dilution cup.

[0181] Controlling the mixing of the first dilution vessel by the transfer component may include steps c to d:

[0182] c: Control the motion mechanism 60 to work, so that the gripper slider 72 moves to the secondary shaft section 743;

[0183] d: Control the rotary motor 79 to work, so that the rotating shaft 74 rotates, thereby driving the gripper slider 72 to shake, and to perform a mixing operation on the first dilution cup.

[0184] Controlling the transfer component to transfer the first dilution cup to the transfer position may include: controlling the transfer component to move to the transfer position along a preset path.

[0185] After step S1023, the liquid dispensing position is vacated, and the second dilution cup can be transferred to this liquid dispensing position.

[0186] Please see Figure 15 Step S103 may include:

[0187] S1031: The control liquid dispensing mechanism draws up the diluent and the first solution based on the amount of diluent and the amount of first solution determined by the second dilution ratio.

[0188] In this step, the liquid dispensing mechanism is moved to the transfer position and the container holding the diluent, respectively, to draw up the first solution and the diluent according to the determined second sample volume a2 and diluent volume b2. The liquid dispensing mechanism can first move to the container holding the diluent to draw up the diluent, and then move to the transfer position to draw up the first solution; or it can be the other way around, first moving to the transfer position to draw up the first solution, and then moving to the container holding the diluent to draw up the diluent. The order is determined by the actual movement path of the liquid dispensing mechanism and is not limited here.

[0189] S1032: Control the liquid adding mechanism to move to the liquid adding position, and dispense the absorbed diluent and the first solution into the second diluent cup located at the liquid adding position to obtain the second solution.

[0190] After this step, the liquid dispensing mechanism can be controlled to move to an area where cleaning can be performed to prepare for the next liquid dispensing.

[0191] S1033: Control the transfer component to remove the second dilution cup and perform a mixing operation on the second dilution cup to mix the second solution evenly.

[0192] This operation can be performed by referring to steps a to d above, and will not be repeated here.

[0193] S1034: Control the transfer assembly to transfer the second dilution cup to the detection area to detect the second solution.

[0194] This step controls the transfer component to transfer the second dilution cup to the detection area along a preset path.

[0195] At this point, the sample dilution operation can be completed. The same principle applies to multiple dilutions. Based on the above steps, the various components and mechanisms can be controlled to operate in a cyclical manner, which will not be elaborated further.

[0196] Please see Figure 16 , Figure 16 This is a schematic block diagram of the circuit structure of an embodiment of the sample detection device of this application. The sample detection device 31 includes a processor 311 and a memory 312 coupled to each other. The memory 312 stores a computer program, and the processor 311 is used to execute the computer program to implement the steps of the sample dilution method embodiments of this application as described above.

[0197] Alternatively, the memory 312 stores a computer program for the above-described sample detection method, and the processor 311 executes the computer program to implement the steps of the various embodiments of the sample detection method of this application as described above.

[0198] For a description of each step of the processing, please refer to the description of each step in the above embodiment of the sample dilution method of this application, and it will not be repeated here.

[0199] In the various embodiments of this application, the disclosed sample dilution method and sample detection device can be implemented in other ways. For example, the embodiments of the sample detection device described above are merely illustrative. For instance, the division of modules or units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection through some interfaces, devices, or units, and may be electrical, mechanical, or other forms.

[0200] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment, depending on actual needs.

[0201] Furthermore, the functional units in the various embodiments of this application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or as a software functional unit.

[0202] If the integrated unit is implemented as a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product, which is stored in a storage medium.

[0203] See Figure 17 , Figure 17 This is a schematic block diagram of the circuit structure of an embodiment of the computer-readable storage medium of this application. The computer storage medium 1000 stores a computer program 1001. When the computer program 1001 is executed, it implements the steps of the sample dilution method and / or the sample detection method of the above-described embodiments of this application.

[0204] The computer storage medium 1000 can be any medium capable of storing program code, such as a USB flash drive, external hard drive, read-only memory (ROM), random access memory (RAM), magnetic disk, or optical disk.

[0205] The above description is merely an embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A sample dilution method, characterized in that, The method includes: Estimate at least one dilution ratio; the at least one dilution ratio includes a second dilution ratio; Based on the target dilution ratio of the sample and the at least one dilution ratio, another dilution ratio is determined; the other dilution ratio includes the first dilution ratio. Adjust the at least one dilution ratio until the other dilution ratio meets the preset requirements, and determine that the current at least one dilution ratio and the other dilution ratio are the dilution ratios used in one of the multiple dilution processes of the sample; The method further includes: The volume of the target solution, the volume range of the solution in the first dilution process, and the ratio of the volume of the first sample used in the first dilution process to the volume of the second sample used in the second dilution process are obtained; wherein the second sample is obtained from the solution after dilution of the first sample; The preset requirement is determined using the volume of the target solution, the solution volume range, the first dilution ratio, the target dilution ratio, and the proportional relationship; wherein the preset requirement is: ,in, This represents the minimum range of solution volumes obtained from the first dilution. This represents the maximum range of solution volumes obtained from the first dilution. The volume of the target solution. This is the first dilution ratio. This is the second dilution ratio. The ratio of the volume of the first sample used in the first dilution process to the volume of the second sample used in the second dilution process.

2. The method according to claim 1, characterized in that, The first dilution ratio and the second dilution ratio are respectively the dilution ratios used in one of the two dilution processes of the sample.

3. The method according to claim 2, characterized in that, The first dilution ratio is the dilution ratio used in the first dilution process of the sample, and the second dilution ratio is the dilution ratio used in the second dilution process of the sample; The estimated at least one dilution ratio includes: Estimated second dilution ratio; Determining another dilution ratio based on the target dilution ratio of the sample and the at least one dilution ratio includes: The first dilution ratio is determined based on the target dilution ratio of the sample and the second dilution ratio; Adjusting the at least one dilution ratio until the other dilution ratio meets the preset requirement includes: Adjust the second dilution ratio until the first dilution ratio meets the preset requirements.

4. The method according to claim 3, characterized in that, The step of adjusting the second dilution ratio until the first dilution ratio meets the preset requirements includes: When the first dilution ratio does not meet the preset requirement, the second dilution ratio is adjusted with a preset adjustment step size; Repeat the step of determining the first dilution ratio based on the target dilution ratio and the second dilution ratio of the sample until the first dilution ratio meets the preset requirements.

5. The method according to claim 4, characterized in that, The estimated second dilution ratio includes: The initial value for the second dilution ratio is 0.5; The step of adjusting the second dilution ratio until the first dilution ratio meets the preset requirements further includes: The second dilution ratio is increased or decreased by a preset adjustment step of 0.1 until the first dilution ratio meets the preset requirements.

6. The method according to claim 3, characterized in that, Determining the first dilution ratio based on the target dilution ratio of the sample and the second dilution ratio includes: The first dilution ratio is calculated using the following formula: in, The target dilution ratio, This is the first dilution ratio. This is the second dilution ratio.

7. A method for detecting a sample, characterized in that, The method includes: The first dilution ratio and the second dilution ratio are determined according to the method of any one of claims 1-5; Based on the first dilution ratio, the diluent and sample solution are drawn up, and the drawn up liquid is spat into the first dilution cup to obtain the first solution; Based on the second dilution ratio, the diluent and the first solution are drawn up, and the drawn up liquid is spat into the second dilution cup to obtain the second solution; The second solution was then tested.

8. The method according to claim 7, characterized in that, The step of drawing up the diluent and sample solution based on the first dilution ratio, and then dispensing the drawn liquid into the first dilution cup to obtain the first solution includes: The control dispensing mechanism draws up the diluent and the sample solution based on the volume of diluent and the volume of sample solution determined by the first dilution ratio; Control the liquid dispensing mechanism to move to the liquid dispensing position, and dispense the aspirated diluent and sample solution into the first dilution cup located at the liquid dispensing position to obtain the first solution; The control transfer component transfers the first dilution cup to the transfer position.

9. The method according to claim 7, characterized in that, The step of taking the diluent and the first solution based on the second dilution ratio, and then dispensing the taken liquid into the second dilution cup to obtain the second solution includes: The control dispensing mechanism draws up the diluent and the first solution based on the amount of diluent and the amount of first solution determined by the second dilution ratio; The liquid dispensing mechanism is controlled to move to the liquid dispensing position, and the absorbed diluent and the first solution are dispensed into the second diluent cup located at the liquid dispensing position to obtain the second solution; The control transfer component removes the second dilution cup and performs a mixing operation on the second dilution cup to mix the second solution evenly; The transfer assembly is controlled to transfer the second dilution cup to the detection area for detection of the second solution.

10. A sample detection device, characterized in that, The sample detection device includes a processor and a memory coupled to each other; the memory stores a computer program, and the processor executes the computer program to implement the steps of the method as described in any one of claims 1-9.

11. A computer-readable storage medium, characterized in that, The computer-readable storage medium stores a computer program that, when executed by a processor, implements the steps of the method as described in any one of claims 1-9.

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