1638results about "Individual particle analysis" patented technology

Methods for detecting diffusing particles are provided which comprise introducing a sample comprising a diffusing particle to an optical microcavity; coupling probe light into the optical microcavity such that the probe light is in resonance with the optical microcavity, wherein the diffusing particle diffuses into an optical mode volume defined by the coupled probe light; and detecting output light from the optical microcavity as a function of time while maintaining resonance, wherein the diffusing particle generates a change the detected output light. Systems for carrying out the methods are also provided.

A system includes a flow cell and a fluidics system under pressure causing sheath and sample biological fluids to flow. The fluidics system includes a gas bubble remover to remove and eliminate gas bubbles in the sheath fluid. The flow cell receives sheath fluid from the fluidics system, wherein the sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives drops of the sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths. A droplet deposition unit system in communication with the deflection chamber receives the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

A blood cell analyzer (100) and a blood cell analysis method (300). The blood cell analysis method (300) comprises: sucking a blood sample to be tested; preparing a first determination sample and obtaining first optical information; preparing a second determination sample and obtaining second optical information; generating a first leukocyte classification scatter diagram and a second leukocyte classification scatter diagram; determining a first counting result of neutrophils of the to-be-detected blood sample based on the first leukocyte classification scatter diagram and the second leukocyte classification scatter diagram; determining an actual distribution area of neutrophils from the first leukocyte classification scatter diagram; based on a reference distribution region of neutrophils of a reference leukocyte classification scatter diagram of the normal blood sample, determining a leaf-division nucleophore distribution region from the actual distribution region; determining a second counting result of the cells falling into the leaf-separating nucleogranulocyte distribution area; and based on the first counting result and the second counting result, determining a counting result of the rod-shaped nuclear granulocytes in the to-be-detected blood sample.

The application belongs to the technical field of animal models for basic scientific research, and particularly relates to a method for establishing an acute lung injury animal model, which comprises the following steps: sending a podophyllotoxin or a podophyllotoxin reagent into a rat body through oral administration or intragastric perfusion to obtain the acute lung injury animal model. The method for establishing the acute lung injury animal model provided by the application uses the podophyllotoxin as an inducer for establishing a mouse acute lung injury model, and the mouse can orally take or intragastrically perfuse the podophyllotoxin to achieve the method, without needing a special medicine injection device, so that the method is simple in medicine taking, few in experimental steps, and does not need complex surgical operations.

The invention discloses a method for acquiring grain orientation characteristics based on optical and height characteristics of an alloy etching structure. The method comprises the following steps: (1) acquiring grain orientation distribution and grain size of an alloy surface structure; (2) obtaining an etching structure on the surface of the alloy; (3) acquiring optical characteristics of the etched structure on the surface of the alloy; (4) obtaining height characteristics of an alloy surface etching structure; and (5) constructing a correlation model of structure grain orientation characteristics based on alloy surface optics and height characteristics, and realizing prediction. Aiming at the test of the alloy grain orientation, the optical and height characteristics of the alloy surface etching structure are synthesized, the measurement of the centimeter-level large sample size or full-area grain orientation of the alloy is easy to realize, and compared with the existing measurement aspect, the method is more efficient and convenient; and more comprehensive and reliable tissue information can be provided for alloy optimization and new material development, and the method has important significance for high-throughput characterization and intelligent manufacturing.

The invention relates to the technical field of optical detection and automatic control, and discloses a computer vision-assisted nano-particle recognition and micro-spectrum automatic measurement system, which comprises a sample positioning module, an imaging module, an optical measurement module and a processing control module, automatic focusing is realized on the basis of a definition evaluation value calculated by performing Fourier transform on the microscopic image; graying, binaryzation and morphological operation are carried out on the focused image so as to identify effective nano-particles and calculate centroid coordinates of the effective nano-particles; according to the coordinates of the center of mass, the X axis and the Y axis of the electric three-axis displacement table are controlled through closed-loop iteration movement, and the measured particles are accurately positioned to the center of the view field. According to the invention, the problems of low manual measurement efficiency, poor repeatability and complex operation in the prior art are solved, and high-flux, full-automatic and high-precision measurement of the spectral characteristics of the nanoparticles is realized.

The invention discloses a positive charge fluorescent nanoprobe targeting BCR-ABL fusion protein and application of the positive charge fluorescent nanoprobe in leukemia single cell drug resistance detection, and relates to the field of biological medicine. According to the invention, the surface of the nanoprobe is subjected to specific modification of a polyethylene glycol hydrophilic polymer chain-bridged targeting molecule, so that the functionalized fluorescent nanoprobe with leukemia subcellular oncogenic fusion protein targeting property is successfully constructed. The probe realizes efficient and accurate targeting of the BCR-ABL fusion protein by regulating a subcellular transport pathway, completes diagnosis and quantitative analysis of drug resistance of the leukemia single-cell BCR-ABL fusion protein by utilizing an endocytosis-transport-exocytosis process of cells, can more comprehensively reveal heterogeneity and drug resistance conditions of BCR-ABL positive cells, and has a good application prospect. And a new technical means is provided for accurate diagnosis and treatment of chronic myelogenous leukemia.

The application discloses a kind of microscale single particle quantitative analysis method and system based on nano-silver signal enhancement, it is related to spectral analysis technical field, the method includes: using laser-induced breakdown spectroscopy system to the carbon black particle target sample is carried out spectral data acquisition, obtains target full spectrum data and is pretreated, and then obtains target spectral data matrix;Variable importance measurement algorithm and continuous projection algorithm are used to the variable in the target spectral data matrix is screened, and target variable is obtained;Target variable is input into carbon black particle metal content prediction model, and the content of metal element in carbon black particle target sample is obtained.The application can quickly and accurately determine the content of metal element in carbon black particle target sample.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The invention relates to the technical field of micro-fluidic biological detection, and discloses a micro-fluidic chip for rapidly detecting bacteria based on multi-mode detection, a preparation method and a detection method.The chip comprises a PDMS runner layer and a glass substrate layer which are sequentially arranged from top to bottom; the PDMS flow channel layer comprises an injection area used for receiving a to-be-detected sample and carrying out pretreatment; the focusing area is used for realizing three-dimensional fluid focusing of the detected particles through a coupling effect of viscoelasticity and an inertial effect; the detection area is used for performing electrical impedance detection and fluorescence detection on the focused particles; the outflow area is used for collecting and outputting the detected sample; the PDMS flow channel layer and the glass substrate layer are bonded through oxygen plasma to form a sealed channel structure, and the sealed channel structure is used for realizing electrical impedance and fluorescence dual-mode synchronous detection of microbial particles in a single flow channel. By optimizing the fluid focusing structure and the electrode design, high-precision synchronous detection of the microbial particles is realized.

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

The invention belongs to the technical field of biological detection, and particularly relates to a bisulfite-free detection kit for specific site 5-methylcytosine and a detection method thereof. According to the method, a TAPbeta treatment method is adopted, 5hmC is sealed through beta-GT, 5mC oxidation is carried out through TET, and 5mC is reduced into dihydrouracil (DHU) through pyridine borane, so that the apparent difference between 5mC and C and 5hmC is converted into single nucleotide polymorphism difference. In addition, a flow microsphere technology based on PNA-assisted click chemical connection starting is established to detect DNA methylation specific sites. A PNA clip is innovatively introduced into click chemical connection, and non-specific connection is inhibited. Target sequence enrichment, signal amplification and signal acquisition are carried out through surface-functionalized magnetic nanoparticles, and the detection sensitivity is improved to fM. The invention provides a new tool for high-specificity and high-sensitivity epigenetic marker detection.

The present invention relates to a system and a method for measuring and analyzing minimal residual disease (MRD) in pediatric B-cell precursor acute lymphoblastic leukemia (B-ALL) using multiparameter flow cytometry (MPFC). The invention finds application in clinical diagnostics and hematology-oncology for quantifying MRD in B-ALL patients with high sensitivity and specificity, needed for risk stratification, monitoring treatment response, and informing therapeutic decisions. The system comprises interconnected subsystems including an acquisition subsystem with an MPFC instrument, a control and file generation subsystem, and an analytical subsystem. The analytical subsystem incorporates modules for sequential data reduction, automated data cleaning, automated unsupervised data clustering, and interactive cluster analysis. Key advantages include high MRD detection sensitivity (e.g., 10⁻⁵ or 0.001%) and high specificity, without reliance on reference samples or supervised machine learning models, making it applicable in laboratories with different measuring equipment and using different panels of antibodies for identification of leukemic cells.

The invention relates to the technical field of micro-fluidic chips, and discloses a multi-fluid effect synergistic three-dimensional single-row focusing micro-fluidic chip and a method for single-line focusing of polystyrene microspheres by using the micro-fluidic chip, the micro-fluidic chip comprises a sample liquid inlet, one end of the sample liquid inlet is provided with an inertia focusing link, and the other end of the sample liquid inlet is provided with an inertia focusing link; a tapered flow guide cone structure is arranged between the inertial focusing link and the sample liquid inlet and is used for inhibiting the initial dispersion and diffusion trend of particles at the sample liquid inlet; the bionic gradient height structure is positioned at one end, far away from the tapered flow guide cone structure, of the inertial focusing link and is used for focusing and compressing the particles in the vertical direction; a sheath fluid focusing area is arranged at one end, far away from the inertial focusing link, of the bionic gradient height structure, and a sheath fluid inlet and a chip mixed liquid outlet are sequentially formed in one end, far away from the bionic gradient height structure, of the sheath fluid focusing area. According to the invention, efficient three-dimensional centered arrangement is realized, dynamic convergence of a particle trajectory from a dispersion state to a central single beam is realized, and a digital focusing regulation capability is realized.

The object of the present invention is to provide a method for screening particles such as cells efficiently. The object can be solved by a method for detecting a particle in droplet by illuminating light with one direction onto the particle which is contained in water in oil while it is flowing together with the oil in a flow path and detecting changes in the intensity of the light signal from the particles over time, characterized in that the particle in the droplet is detected by measuring the scattered light of the droplet and the scattered light of the particle.

Described herein are systems, devices, and methods allowing for a subset of images to be used to establish a population count for biological material (e.g., blood cells).

The invention relates to a method and system for detecting cells of a first cell type (1) in a biopsy sample in which a plurality of cells of different cell types (2, 3) are present. The invention further relates to a device for carrying out a magnetic field measurement on cells of a biopsy sample that are labeled with magnetic nanoparticles. The method comprises the following method steps: carrying out tissue dissociation of the biopsy sample in order to singulate the cells present in the biopsy sample; supplying magnetic nanoparticles (11) to the singulated cells in order to selectively label the cells of the first cell type (1); removing the excess magnetic nanoparticles (11); introducing the cells into a microchannel (5), the microchannel (5) having a cross section which allows only one labeled cell (1) to pass through at a time; carrying out a magnetic field measurement in the microchannel (5) by means of a quantum magnetic field sensor (6) based on the F-center principle; and sorting out the labeled cells (1) after they have passed through the microchannel (5).

The present disclosure provides improved and useful techniques for cross-standardization of flow cytometry instruments and, particularly, spectral flow cytometry instruments. Aspects of the disclosure include methods of calibrating a flow cytometer having a plurality of fluorescence channels. Methods of interest utilize calibration sets of bead populations, wherein each bead population of the calibration set includes a different fluorophore attached to a surface thereof and the calibration set includes a number of bead populations that is less than the number of fluorescence channels of the flow cytometer. Flow cytometers, non-transitory computer-readable storage media, and kits including, e.g., calibration sets of bead populations for carrying out the subject methods are also provided.

The present invention relates to an automatic real-time label-free holography-activated sorting of the cell's technique. The technique provides high-discriminative power on the level of the individual cell. The technique includes rapid automated cell processing during cell visualization and flow, with high discriminative power on the level of the individual cell. The technique may be useful in detection of cancer and to identify different stages of oncogenesis.

A bio-substance analysis method using a sensing substrate having a fluid channel is disclosed. The method includes mixing retroreflective particles with a detection solution containing a target bio-substance, wherein a first bio-recognition substance selectively reacting with the target bio-substance is modified on the retroreflective particles; placing the sensing substrate so that a bottom is located under a cover in a direction of gravity; injecting the detection solution containing therein the retroreflective particles into a fluid channel and maintaining the solution in the channel for a first time duration; turning the sensing substrate upside down so that the bottom is located above the cover in the direction of gravity and maintaining the sensing substrate in the turned state for a second time duration; irradiating light into the fluid channel through the bottom; and generating and analyzing an image based on light retroreflected from the retroreflective particles.

An object of the present invention is to provide a technique capable of coping with sensitivity that changes for various reasons when measuring the size of particles contained in a liquid sample using light. A particle measurement device according to the present invention calibrates a size of a particle included in a sample using a reference sample, the reference sample including a reference sample window having the same material quality and thickness as those of a sample container window and including a substance having a reflectivity similar to that of the sample, and calibrates the sample size using the reflectivities of the substance measured at different two time points (see FIG. 9).

The application discloses a flow cytometry sample processing system and belongs to the technical field of automatic processing of biological samples. The flow cytometry sample processing system comprises a machine table, a flow tube block placing structure, a transfer mechanical arm, a shaking module and a centrifuge. The flow tube block placing structure is arranged on the machine table, a flow tube block is arranged on the flow tube block placing structure, a flow tube is arranged on the flow tube block, the transfer mechanical arm provided with a clamping jaw can integrally transfer the flow tube block provided with a clamping hole to the shaking module and the centrifuge, and the whole process automation is truly realized, and the sample centrifugal processing efficiency is improved.

The present disclosure provides a contact-less detection using a cross-cavity device where a small dielectric sample is placed at its center. The optical properties of the sample, such as the Kerr and Faraday rotation, or polarizability, manifest in the coupling between the cavities' electromagnetic modes and in the shift of their resonant frequencies. By calculating the dynamics of what are referred to herein as geometrical photonic states, a measuring protocol is formulated based on the quantum metric that maximizes the Fisher information and isolates the individual components of the complex dielectric function.

Herpesviridae glycoprotein B (gB) polypeptides comprising a modified ectodomain is stabilized in the prefusion state, enabling development of inhibitors and vaccines directed against these viral pathogens. Modifications to DI, DII, and DV subdomains are important to stabilization of gB in the prefusion state, and additional modifications result in a further improved stabilization of gB in the prefusion state. The gB can be derived from an Epstein Barr Virus (EBV), a Human Cytomegalovirus (CMV), a Human Herpesvirus 6 (HHV6), a Herpes Simplex Virus 1 (HSV1), or a Varicella Zoster Virus (VZV). Polypeptides, nucleic acid constructs, compositions, and methods of using same to elicit an immune response are described.

Aspects of the present disclosure include methods for assessing the suitability of a fluorochrome panel for use in generating flow cytometer data. Methods according to the present disclosure include identifying two or more fluorochromes in a fluorochrome panel that would be associated with variance in flow cytometer data generated using the fluorochrome panel by: obtaining a negative population and a positive population for one or more single-stained fluorochromes in the fluorochrome panel, calculating a covariance matrix for each of the negative population and the positive population, calculating an unmixed covariance matrix of a positive population for each fluorochrome in the fluorochrome panel, calculating a spread metric from the unmixed covariance matrix and identifying that the two or more fluorochromes are associated with variance in flow cytometer data based on the calculated spread metric. Methods include identifying the two or more fluorochromes in the fluorochrome panel by assessing the collinearity between the spectra of each of the two or more fluorochromes based at least in part on a variance decomposition proportion (VDP) of the spectra that is above a set threshold. Systems and non-transitory computer-readable storage media configured to carry out the subject methods are also provided.

Aspects of the present disclosure include methods for modulating the intensity profile of a laser beam. The method, according to certain embodiments, includes illuminating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining from the captured image an intensity profile of the output laser beam along a horizontal axis, and adjusting one or more parameters of a waveform input to the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor, and a waveform generator, as well as a non-transitory computer-readable storage medium having instructions for practicing the subject methods, are also described.

In the preparation method of the visible component detection dry reagent, the redissolving reagent and the microscopic examination sample, the visible component detection dry reagent is formed by drying a liquid dyeing reagent; the liquid staining reagent comprises a staining agent and an acid-base regulator; the pH regulator is used for regulating the pH value of the liquid dyeing reagent; the liquid dyeing reagent is filtered before being dried. Before drying, dividing the liquid dyeing reagent into small parts, and drying separately; the liquid dyeing reagent is divided into single parts with set volume, and then is dried to remove moisture. The single part of the set capacity is 300 ul to 3000 ul per piece. The visible component is used for detecting the dry reagent, so that the reagent is more convenient to store and transport, and the quality guarantee period is longer.

The invention provides an application of a pair of immune costimulatory factors TNFSF9 / TNFRSF9 as markers in preparation of a product for early prediction of preeclampsia, and the immune costimulatory factors comprise a tumor necrosis factor superfamily member 9 (TNFSF9) and a tumor necrosis factor receptor superfamily member 9 (TNFRSF9). The product is used for carrying out early warning on the preeclampsia occurrence risk 20 weeks before pregnancy. The product is used for detecting the immune costimulatory factor through at least one of serum, plasma, whole blood and placental tissue. The invention aims to improve the early prediction efficiency of preeclampsia by detecting the abnormal expression level of the pair of markers.