The invention relates to a IL-6/PCT combined detection time-resolved fluorescence immunochromatography quantitative detection kit and a detection method, which utilize the sensitivity of a time-resolved fluorescence immunochromatography technology and rare earth vanadate nano fluorescence labeling materials and combine a dry-type immunofluorescence analyzer to realize high sensitivity, accurate quantification, simplicity and convenience and rapidness. The detection kit can accurately and quantitatively detect the contents of interleukin 6 and procalcitonin in human serum, plasma and whole blood samples, the samples can be processed by the blood filtering membrane sample pad without pretreatment and dilution by diluent, the fluorescence interference of the samples can be avoided by using atime-resolved fluorescence microsphere detection technology, the rare earth vanadate nano material has the advantages of low background, strong fluorescence signal, high signal-to-noise ratio and thelike, the rare earth vanadate nano particles are connected with the antibody through a covalent bond, and the labeled product is stable, so that the detection kit has the characteristics of wide detection range, high sensitivity, high accuracy, quick and simple detection and the like.