32 results about "A hemoglobin" patented technology

The utility model relates to the technical field of livestock and poultry blood utilization, in particular to a hemoglobin low-temperature extraction device which adopts two-stage separation and integrated design, is high in integration level, suitable for small-scale hemoglobin extraction work and good in practicability. Comprising a tank; the storage crushing tank is installed on the upper portion of the tank body, the liquid inlet pipe is installed on the top of the storage crushing tank, the first centrifugal separation tank is installed in the middle of the tank body, the storage crushing tank is communicated with the first centrifugal separation tank through a pipeline, and the second centrifugal separation tank is installed in the middle of the storage crushing tank through a pipeline. A residue discharging pipe is installed on the side wall of the first centrifugal separation tank, the upper end of the conveying pipe is communicated with the first centrifugal separation tank, the second centrifugal separation tank is installed on the lower portion of the tank body, a first liquid discharging pipe is installed on the side wall of the second centrifugal separation tank, and the upper end of the second liquid discharging pipe is communicated with the bottom of a second separation cavity of the second centrifugal separation tank. The upper end of the second liquid discharging pipe is located on the inner side of the inner end of the first liquid discharging pipe.

To provide a color and shape change monitoring system capable of accurately monitoring the color and shape change of a human body caused by a blood flow change.SOLUTION: The image processing apparatus includes a three dimensional point cloud data acquisition unit configured to acquire three dimensional point cloud data of a measurement target portion of a human body, a color data acquisition unit configured to acquire color data of the measurement target portion, a color-shape-data generation unit configured to generate color-shape data of the measurement target portion in which the color data is associated with the three dimensional point cloud data, and a pigment component separation unit configured to separate the color-shape data into a hemoglobin pigment component, a melanin pigment component, and a shade component.SELECTED DRAWING: Figure 1

The application discloses a hemoglobin camel-derived nanobody and a preparation method and application thereof. The hemoglobin camel-derived nanobody is an amino acid sequence shown in SEQ ID NO: 7, is a hemoglobin camel-derived nanobody specific to hemoglobin derived from a bactrian camel, has small molecules, can easily penetrate, has high specificity, good stability, is easy to produce and transform, has wide application prospects, and can be applied to the fields of biomedical research, clinical diagnosis and treatment and the like.

The application relates to a biochemical analyzer and a detection method for detecting glycated hemoglobin. The hemoglobin concentration and the glycated hemoglobin concentration of a to-be-detected whole blood sample are obtained, and the content proportion of the glycated hemoglobin is calculated according to the hemoglobin concentration and the glycated hemoglobin concentration. The hemoglobin concentration is obtained by the following steps: obtaining a first absorbance of a to-be-detected sample liquid at a hemoglobin measurement wavelength, wherein the to-be-detected sample liquid is prepared by mixing a to-be-detected whole blood sample, a hemolytic agent and a hemoglobin detection reagent; obtaining at least two second absorbances of the to-be-detected sample liquid at at least two different correction wavelengths, wherein the correction wavelengths are designed so that the absorbance of a lipid interferent at the correction wavelength is greater than the absorbance of hemoglobin at the correction wavelength; correcting the first absorbance according to the second absorbance; and determining the hemoglobin concentration according to the corrected first absorbance. The application can improve the accuracy of hemoglobin detection.

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.

In at least one embodiment, a hemoglobin measuring device may include a plurality of sensors, a display configured to present information, and a computing device in communication with the sensors and display and having a processor and a memory storing instructions executable by the processor. The instructions may include instructions to receive, from the sensors, measurements of one or more forms of glycated hemoglobin in a blood samples, determine a total fetal hemoglobin value in the blood sample by performing a peak area summation of all forms of fetal hemoglobin, subtract the total fetal hemoglobin value from 100 to derive a total adult hemoglobin value, determine a glycated fetal hemoglobin value, compare the glycated fetal hemoglobin value to a reference range to obtain a comparison, determine a baseline glucose control value based on the comparison, and output the baseline glucose control value and the comparison to a display for medical evaluation.

The invention relates to a hemoglobin oxygen carrier based on small molecule modification as well as a preparation method and application thereof. The hemoglobin oxygen carrier comprises hemoglobin and N-fluoromethoxycarbonyl diphenylalanine, the hemoglobin forms the hemoglobin oxygen-carrying carrier through the non-covalent bond acting force between the hemoglobin and the N-fluoromethoxycarbonyl diphenylalanine. According to the hemoglobin oxygen carrier disclosed by the invention, a co-assembly strategy of N-fluoromethoxycarbonyl diphenylalanine and hemoglobin is rapidly implemented by taking a non-covalent bond acting force as a driving force. The hemoglobin oxygen-carrying carrier prepared by the process greatly shortens the stabilization time of hemoglobin molecules on the basis of ensuring the stability of molecular chemical groups, has good biocompatibility and injectable application characteristics on the basis of the characteristics of non-covalent acting force, and provides a new foundation for research and development of a new generation of hemoglobin oxygen-carrying carriers. And an engineering design thought and a theoretical basis are provided.

This application relates to the field of medical image processing technology, specifically to a hemoglobin emphasis device and method for endoscopes. The device includes a light source unit; an imaging unit for acquiring reflected light signals from a target area under multispectral illumination and outputting a raw Bayer format image or an RGB color image; and an image processing unit for receiving and processing the output Bayer format image or RGB color image, comprising at least: a spectral reconstruction module for calculating and reconstructing a single-band image signal using a spectral reconstruction model; a hemoglobin concentration calculation module configured to calculate a relative hemoglobin concentration based on the image signal; and an image enhancement module configured to perform pixel-level gain adjustment on the raw color image or the processed image, convert it to a set color space for contrast mapping, and finally output a hemoglobin-emphasized image. The image output by this invention can significantly improve the contrast between blood vessels and surrounding tissues.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

An object of the present invention is to provide an eluent for suppressing bimodality of hemoglobin F which is a β - thalassemia disease marker in hemoglobin analysis in cation exchange chromatography and stably separating hemoglobin F without depending on the type of diluent, and a method for separating hemoglobin F and a method for diagnosing hemoglobin F using the eluent.SOLUTION: In the analysis of a hemoglobin sample using cation exchange chromatography, the problem is solved by an eluent having a pH of 5.0 to 6.0, which is passed when separating and quantifying hemoglobin F from the hemoglobin sample, wherein the eluent contains at least one or more pH buffers, and a relationship between the pH of the eluent and a pKa value in a range of 5.0 to 7.0 among pKa values indicated by the pH buffers is a relationship of pH ≤ pKa ≤ pH + 1.0.SELECTED DRAWING: Figure 1

The invention provides a color correction method and apparatus, an electronic device and a computer readable storage medium. The method comprises the steps of obtaining a target face image; determining a melanin dominant component and a hemoglobin dominant component in the target face image; determining a skin color type corresponding to the target face image according to the melanin dominant component and the hemoglobin dominant component; determining a correction parameter corresponding to the skin color type; correcting a preset color correction matrix through the correction parameter to obtain a target color correction matrix; and correcting the target face image through the target color correction matrix. The melanin dominant component and the hemoglobin dominant component in the target face image are determined, so that the skin color features of the user in the target face image are determined, the specific skin color type is determined, the color correction matrix is adjusted according to the skin color type, and then correction is performed through the target color correction matrix, so that the color correction accuracy is improved. And different skin color types can be adapted.

According to the real-time bleeding monitoring method and device based on the spectrometry, the equipment and the storage medium, the drainage container is clamped through the intelligent detection clamp, the instantaneous flow and the corresponding actual absorbance data of the drainage container under the reference wavelength and the multiple different specified wavelengths are collected in real time, and collection is convenient and fast; according to all actual absorbance data and the hemoglobin concentration quantitative analysis model, the current hemoglobin concentration is determined objectively, accurately and efficiently; according to the current hemoglobin concentration and the instantaneous flow, the hemoglobin loss rate and the total hemoglobin loss amount are determined in real time, according to the hemoglobin loss rate and the total hemoglobin loss amount, first grading early warning is carried out, and systolic pressure and heart rate are collected in real time; according to the hemoglobin loss total amount, the systolic pressure and the heart rate, the second grading early warning is carried out, and real-time precise double grading early warning is convenient for related personnel to timely and accurately evaluate the bleeding condition and take corresponding measures.

The invention discloses a hemoglobin-based micro-nano reactor as well as a preparation method and application thereof, belongs to the technical field of biochemistry, and particularly relates to a hemoglobin-based micro-nano reactor which is formed by self-assembly of hemoglobin-polylactic acid conjugates. The hemoglobin-based micro-nano reactor has a cell-like space structure and has multiple functions of a drug delivery system and a micro-nano reactor, hemoglobin biomolecules in the hemoglobin-based micro-reactor are utilized to catalyze atom transfer radical polymerization reaction, and the activity of the hemoglobin-based micro-nano reactor is improved under the condition of not additionally adding a catalyst or gene modification. The micro-nano reactor has the advantages of simplicity and convenience in synthesis, good biocompatibility, various catalytic polymers and the like, and is suitable for the fields of cell engineering, biological material preparation, fluorescence labeling, production of polymers by a fermentation method and the like.

A non-invasive hemoglobin monitoring device comprises processing circuitry coupled to an infrared receiver and a tactile button. The processing circuitry may be configured to detect a finger positioned in a finger placement area based on activation of the tactile button, acquire multiple sensor readings from the infrared receiver as the finger is positioned, calculate an average value from the multiple sensor readings, and determine a hemoglobin concentration based on the average value. A display screen coupled to the processing circuitry may display the hemoglobin concentration. The device may provide accurate hemoglobin measurements without requiring invasive blood sampling procedures, utilizing optical sensing technology and tactile feedback mechanisms to ensure proper finger positioning during measurement operations.

The utility model provides a hemoglobin and transferrin joint detection test strip and a reagent plate, and belongs to the technical field of biological detection. The colloidal gold pad is coated with a conjugate of an anti-human hemoglobin monoclonal antibody and colloidal gold and a conjugate of an anti-human serum transferrin monoclonal antibody and colloidal gold; the first detection line T1 is coated with at least one of an anti-human hemoglobin specific antibody or an anti-human serum transferrin specific antibody; the hemoglobin and transferrin combined detection test strip is coated with at least one second detection line T2 line of an anti-human hemoglobin specific antibody or an anti-human serum transferrin specific antibody, and is coated with an anti-mouse IgG antibody quality control region C line; the detection of serum protein and transferrin can be rapidly realized at one time, the use amount of detection materials in actual combats is saved, and the kit conforms to the operation habits of public security actual combats and is more suitable for difficult bloodstain detection.

The application provides a hemoglobin concentration measuring endoscope device and method, and relates to the technical field of endoscopes. The target tissue is alternately illuminated by narrow-band light combination 1 and narrow-band light combination 2, color image 1 and color image 2 are obtained, reflection information sensitive to shallow, middle and deep hemoglobin is extracted therefrom, shallow, middle and deep hemoglobin indexes are obtained by using scattering correction and background light correction, the hemoglobin indexes are normalized and corrected by using a hemoglobin absorption coefficient, and finally, the hemoglobin concentration is calculated based on the corrected hemoglobin indexes and a concentration distribution map is output. The application can more accurately reflect the comprehensive distribution of blood vessels in the shallow, middle and deep tissues, and further more accurately measure the hemoglobin concentration of the tissues, while ensuring the need for real-time image observation of the endoscope.

A method (100) for on-site detection at trace levels in a water sample of a fecal pigment, i.e. a metabolite or degradation product of a hemoglobin, such as urobilin and / or stercobilin is suggested, comprising: (i) providing a zinc salt and silane functionalized fibrous substrate (2), in particular a zinc salt and silane functionalized glass fiber substrate (2); (ii) wetting a detection region (3) of the zinc salt functionalized fibrous substrate (2) with the water sample; (iii) exposing the detection region (3) to an excitation light, the excitation light typically comprising a wavelength in a range of about 450 – 490 nm, particularly of about 475 nm, a full width half maximum of the excitation light typically being at most about 25 nm; (iv) detecting a fluorescence light emitted in response to exposing the detection region (3) to the excitation light; and at least one of: (v) detecting, based on the fluorescence light, a presence of the fecal pigment in the water sample; and (vi) correlating an intensity of the detected fluorescence light with a calibration data set for determining a concentration of the fecal pigment in the water sample.

The application discloses a hemoglobin analysis anticoagulant sample agglutination detection equipment, and relates to the technical field of biomedical detection, which comprises a casing, a blood collection tube and a machine cover rotatably installed on the casing, a plurality of laser receiving plates rotatably installed on the machine cover and a light blocking cylinder fixedly installed in the casing; a support is installed in the casing through a damping support, a motor is fixedly installed on the support, a rotating disc is rotatably installed on the support, an object placing disc is fixedly installed on the rotating disc, and a plurality of tube rack stations for storing the blood collection tubes are installed on the object placing disc. The application has the advantages that the centrifugal light transmittance is used to detect the agglutination condition of the blood sample, a plurality of batches of samples can be detected at a time, the detection efficiency is higher, the detection has higher real-time performance, the detection result is more accurate, the level position of the agglutination can be judged, and the type of the agglutination can be judged according to the agglutination position.

The present invention relates to a polynucleotide construct comprising a hemoglobin subunit alpha-hemoglobin subunit beta (HBA-HBB) hybrid gene construct for enhancing the potency of gene therapy for the β-hemoglobinopathies, particularly β-thalassemia and sickle cell disease (SCD). The polynucleotide construct comprising elements that are operatively connected to each other in the following sequence: HBB locus control region (HBB-LCR), HBA promoter, and β-like globin gene. Additionally, the HBA-HBB gene construct further comprises a microRNA-based short hairpin RNA (shRNAmir) targeting one of the endogenous HBA or HBB genes to increase the ratio of vector-encoded HBBT87Q to endogenous HBA and β-like globin chains while maintaining high vector titers.

The invention relates to the field of biological medicine, in particular to a drug combination which comprises a hemoglobin oxygen carrier and a radionuclide drug, and the drug combination can effectively play a role in inhibiting tumor cell proliferation. Particularly, the invention relates to application of hemoglobin oxygen carrier combined radionuclide medicine in treatment of prostatic cancer.

Techniques and apparatuses are described for determining an estimated cardiac output of a patient during a dialysis treatment. In one embodiment, for example, an apparatus can include a memory and logic coupled with the memory. The logic can be configured to determine an upper body oxygen consumption of the patient, determine, during the dialysis process, a hemoglobin concentration and a venous oxygen saturation, as measured via an optical blood monitor operably coupled to an extracorporeal circuit of a dialysis system performing the dialysis process, an arterial oxygen saturation as measured via a pulse oximeter operably coupled to the patient, an arterial-venous oxygen content difference based on the arterial oxygen saturation and the venous oxygen saturation, and an upper body blood flow (UBBF) as (the upper body oxygen consumption) / (the arterial-venous oxygen content difference), and determine a treatment recommendation based on the upper body blood flow. Other embodiments are also described.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

The invention discloses a monoclonal antibody for glycosylated hemoglobin detection, a preparation method thereof and a kit. The preparation method comprises the following steps: preparing complete antigens of saccharified hemoglobin and non-saccharified hemoglobin, respectively immunizing mice, and obtaining a hybridoma cell bank through cell electrofusion and HAT screening; screening three monoclonal antibodies by using an ELISA plate coated with HbA0-BSA and HbA1c-BSA through an enzyme-linked immunosorbent assay, wherein the three monoclonal antibodies comprise a first monoclonal antibody for specifically recognizing a covalent binding region of valine and saccharides at the N end of a hemoglobin beta chain and a second monoclonal antibody for specifically recognizing 1-6 amino acid regions at the N end of an unglycosylated hemoglobin beta chain; and a third monoclonal antibody that recognizes a hemoglobin alpha chain epitope. The kit comprises the antibody, and the percentage of the glycosylated hemoglobin is calculated by respectively detecting the concentration of the glycosylated hemoglobin and the concentration of the non-glycosylated hemoglobin by adopting a magnetic particle chemiluminescence method. The antibody is good in specificity and high in affinity, and the kit is high in hemoglobin variant recognition capacity and suitable for diabetes diagnosis and blood glucose monitoring in hospitals of all levels.

To provide assays and genotyping methods for the detection of hemoglobin A (HBA). [Solution] This disclosure relates to a hemoglobin A (HBA) assay and an HBA genotype determination tree matrix for implementation in clinical trials. More specifically, the embodiments relate to obtaining raw data from an HBA assay performed on multiple samples, calculating a first set of probe ratios for each sample based on the raw data, identifying the number of reference samples to be combined as a synthetic reference sample based on the first set of probe ratios, calculating a second set of probe ratios for each of the multiple samples based on the raw data and the synthetic reference samples, and determining the HBA genotype of each sample by a decision tree matrix based on the second set of probe ratios for each sample and the copy number call threshold of the sample probe / reference probe ratio associated with each probe of the multiple probes.

The invention provides an anemia detection device and an anemia detection model training method and device, and relates to the technical field of medical auxiliary equipment and the technical field of image processing, and the anemia detection device comprises an acquisition module which is used for acquiring a face image of a target user; the identification module is used for identifying a region of interest of a target image in the face image to obtain a region of interest image, and the region of interest is a lip region, a cheek region, a lip and cheek region or a full face region; and the detection module is used for performing anemia detection on the image of the region of interest through a pre-trained anemia detection model to obtain an anemia detection result corresponding to the target user, and the anemia detection result is a three-classification anemia degree detection result and / or a hemoglobin detection result. According to the invention, accurate grading of anemia severity and more accurate quantification of hemoglobin detection results are realized, and a more accurate basis is provided for differentiated treatment and clinical decision.

The invention relates to the technical field of red blood cell life detection, in particular to a self-service red blood cell life detection system and method based on an intelligent vending terminal, and the system comprises a man-machine interaction module which is used for guiding a user to purchase and wear a gas collection accessory and putting a finger into a detection groove; the central processing and control module is used for introducing the expiration sample into an analysis cabin of the endogenous carbon monoxide concentration test module and calculating to obtain a red blood cell lifetime value of the user; the endogenous carbon monoxide concentration test module is used for measuring the endogenous carbon monoxide concentration of an expiration sample through electrochemical detection or a non-dispersive infrared spectroscopy and transmitting a measurement result of the endogenous carbon monoxide concentration to the central processing and control module; and the hemoglobin concentration test module is used for measuring the hemoglobin concentration of the user by detecting the finger placed in the detection groove and transmitting the measurement result of the hemoglobin concentration to the central processing and control module. The red blood cell life detection efficiency can be improved.

The application discloses a hemoglobin-resistant Taq DNA polymerase mutant and a construction method thereof, relates to the field of biology, and has the technical scheme as follows: the amino acid sequence coded by the nucleotide sequence of Taq DNA polymerase shown in SEQ ID NO. 1 is subjected to mutation, and the mutant contains at least one mutation or all mutations in the S623D and E721A sites. On the basis of natural Taq DNA polymerase, the molecular structure of the Taq DNA polymerase is reformed through rational design and a site-directed mutation biological technology, so that the Taq DNA polymerase mutant is more suitable for PCR amplification of samples containing hemoglobin and the like than the wild type, and is more beneficial to the accuracy of result evaluation of samples containing hemoglobin inhibitors.

The present invention relates to a hemoglobin-based oxygen carrier comprising a complex formed by chemically binding hemoglobin, PEG, and albumin, and a preparation method therefor. Furthermore, the present invention relates to an artificial blood composition comprising the hemoglobin-based oxygen carrier as an active ingredient. The hemoglobin-based oxygen carrier according to the present invention overcomes issues of low oxygen saturation and CO binding, and exhibits excellent biocompatibility and stability, thereby demonstrating oxygen transport capacity and oxidative stability comparable to or superior to those of natural blood.

The application provides a hemoglobin cascade response nano vesicle and a preparation method and application thereof, and belongs to the technical field of biological medicine. The first hemoglobin response nano vesicle (DecAS-PC@NM) is designed, and disease-specific treatment is realized through cascade reaction of two functional phospholipids. First, hemoglobin activates artemisinin-modified phospholipid (A-PC) to generate active oxygen, the active oxygen oxidizes the sulfide structure in another phospholipid (S-PC), and the transformation from hydrophilicity to hydrophobicity is caused, so that the vesicle is disintegrated. Through co-assembly with neutrophil membranes, the chemotaxis to inflammatory sites is further enhanced. In vitro studies show that DecAS-PC@NM has unique hemoglobin responsiveness, and in vivo data emphasize its targeting to ischemic cores and significant therapeutic effect. This innovative hemoglobin-triggered phospholipid cascade reaction provides a promising strategy for the treatment of myocardial ischemia-reperfusion injury.

The application relates to the technical field of medical image processing, in particular to a hemoglobin emphasis device and method for an endoscope. The device comprises a light source part; a shooting part used for collecting reflected light signals of a target area under the multispectral illumination and outputting original Bayer format images or RGB color images; an image processing part used for receiving and processing the outputted Bayer format images or RGB color images, which at least comprises: a spectrum reconstruction module used for calculating and reconstructing single-band image signals by using a spectrum reconstruction model; a hemoglobin concentration calculation module configured to calculate relative hemoglobin concentration based on the image signals; and an image enhancement module configured to perform pixel-level gain adjustment on the original color images or the processed images, convert to a set color space for contrast mapping, and finally output hemoglobin emphasized images. The outputted images can significantly improve the contrast between blood vessels and surrounding tissues.