58 results about "A hemoglobin" patented technology

The invention discloses a hemoglobin-resistant Taq DNA polymerase mutant and a construction method thereof, and relates to the field of biology, and the hemoglobin-resistant Taq DNA polymerase mutant is characterized in that a nucleotide sequence for coding the Taq DNA polymerase is shown as SEQ ID NO.1, and the mutant comprises at least one mutation or all mutations selected from S623D and E721A sites. On the basis of natural Taq DNA polymerase, the molecular structure of the Taq DNA polymerase is modified through rational design and combination with a site-specific mutagenesis biotechnology, so that the Taq DNA polymerase mutant is more suitable for PCR amplification of samples containing hemoglobin and the like than a wild type, and the result judgment accuracy of a sample containing a hemoglobin inhibitor is more facilitated.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

The utility model relates to the technical field of livestock and poultry blood utilization, in particular to a hemoglobin low-temperature extraction device which adopts two-stage separation and integrated design, is high in integration level, suitable for small-scale hemoglobin extraction work and good in practicability. Comprising a tank; the storage crushing tank is installed on the upper portion of the tank body, the liquid inlet pipe is installed on the top of the storage crushing tank, the first centrifugal separation tank is installed in the middle of the tank body, the storage crushing tank is communicated with the first centrifugal separation tank through a pipeline, and the second centrifugal separation tank is installed in the middle of the storage crushing tank through a pipeline. A residue discharging pipe is installed on the side wall of the first centrifugal separation tank, the upper end of the conveying pipe is communicated with the first centrifugal separation tank, the second centrifugal separation tank is installed on the lower portion of the tank body, a first liquid discharging pipe is installed on the side wall of the second centrifugal separation tank, and the upper end of the second liquid discharging pipe is communicated with the bottom of a second separation cavity of the second centrifugal separation tank. The upper end of the second liquid discharging pipe is located on the inner side of the inner end of the first liquid discharging pipe.

To provide a color and shape change monitoring system capable of accurately monitoring the color and shape change of a human body caused by a blood flow change.SOLUTION: The image processing apparatus includes a three dimensional point cloud data acquisition unit configured to acquire three dimensional point cloud data of a measurement target portion of a human body, a color data acquisition unit configured to acquire color data of the measurement target portion, a color-shape-data generation unit configured to generate color-shape data of the measurement target portion in which the color data is associated with the three dimensional point cloud data, and a pigment component separation unit configured to separate the color-shape data into a hemoglobin pigment component, a melanin pigment component, and a shade component.SELECTED DRAWING: Figure 1

The present application relates to a non-invasive hemoglobin detection method based on hyperspectral image reconstruction, belonging to the field of physiological signal detection. The present application irradiates human skin tissue by using a visible light source, simultaneously uses a hyperspectral camera and an RGB camera to simultaneously collect images of the irradiated skin area as a training set, trains a deep learning model, and realizes hyperspectral reconstruction of ordinary RGB images. A hemoglobin extraction feature extraction model for the visible light range diffuse reflectance spectrum is established using an algorithm, and the real hemoglobin content of the measured human body is output through a hemoglobin correction model.

The application discloses a hemoglobin camel-derived nanobody and a preparation method and application thereof. The hemoglobin camel-derived nanobody is an amino acid sequence shown in SEQ ID NO: 7, is a hemoglobin camel-derived nanobody specific to hemoglobin derived from a bactrian camel, has small molecules, can easily penetrate, has high specificity, good stability, is easy to produce and transform, has wide application prospects, and can be applied to the fields of biomedical research, clinical diagnosis and treatment and the like.

A recombinant hemoglobin, a nucleic acid encoding the recombinant hemoglobin, and a preparation method therefor are provided to address the current problems of complex processes, high production costs, and low yields that exist for improving a hemoglobin having a stable tetrameric structure. The recombinant hemoglobin comprises: two or more units of recombinant hemoglobins, the units of recombinant hemoglobins each comprise four polypeptide chains, and the polypeptide chains are two α chains and two β chains. The amino acid sequences of the four polypeptide chains of each unit of recombinant hemoglobin are linked by means of intra-unit linker peptides to form the unit of recombinant hemoglobin, and two or more of the units are linked by means of inter-unit linker peptides.

Disclosed are an apparatus and method for estimating a target component value. The apparatus for estimating a target component value may include: a sensor configured to obtain a spectrum of light scattered or reflected from an object; and a processor configured to correct a first reflectance value of the spectrum based on a melanin index; obtain a second reflectance value based on the correction of the first reflectance value; convert the second reflectance value into an absorbance value; estimate the target component value based on the absorbance value; and correct the target component value based on a hemoglobin index.

The application relates to a biochemical analyzer and a detection method for detecting glycated hemoglobin. The hemoglobin concentration and the glycated hemoglobin concentration of a to-be-detected whole blood sample are obtained, and the content proportion of the glycated hemoglobin is calculated according to the hemoglobin concentration and the glycated hemoglobin concentration. The hemoglobin concentration is obtained by the following steps: obtaining a first absorbance of a to-be-detected sample liquid at a hemoglobin measurement wavelength, wherein the to-be-detected sample liquid is prepared by mixing a to-be-detected whole blood sample, a hemolytic agent and a hemoglobin detection reagent; obtaining at least two second absorbances of the to-be-detected sample liquid at at least two different correction wavelengths, wherein the correction wavelengths are designed so that the absorbance of a lipid interferent at the correction wavelength is greater than the absorbance of hemoglobin at the correction wavelength; correcting the first absorbance according to the second absorbance; and determining the hemoglobin concentration according to the corrected first absorbance. The application can improve the accuracy of hemoglobin detection.

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.

The invention relates to the technical field of biological detection, in particular to a hemoglobin content prediction method and equipment, and the method comprises the following steps: processing multiple groups of light absorption signals obtained based on a hemoglobin solution to obtain time sequence data; inputting the time sequence data into a one-dimensional convolutional neural network to extract multi-scale local features, and inputting the multi-scale local features into an attention transformation network to extract global information and local information to obtain comprehensive attention features; extracting a relationship between different peaks in the target signal corresponding to the time sequence data through a graph attention network to obtain a peak-to-peak relationship feature; and inputting the comprehensive attention features and the peak-to-peak relationship features into a feature fusion network for fusion, and calculating a hemoglobin content index based on the obtained fusion features. The method comprehensively considers the influence of the overall and local characteristics, the peak-to-peak relationship and the like, so that the hemoglobin content index can be accurately detected.

In at least one embodiment, a hemoglobin measuring device may include a plurality of sensors, a display configured to present information, and a computing device in communication with the sensors and display and having a processor and a memory storing instructions executable by the processor. The instructions may include instructions to receive, from the sensors, measurements of one or more forms of glycated hemoglobin in a blood samples, determine a total fetal hemoglobin value in the blood sample by performing a peak area summation of all forms of fetal hemoglobin, subtract the total fetal hemoglobin value from 100 to derive a total adult hemoglobin value, determine a glycated fetal hemoglobin value, compare the glycated fetal hemoglobin value to a reference range to obtain a comparison, determine a baseline glucose control value based on the comparison, and output the baseline glucose control value and the comparison to a display for medical evaluation.

The invention discloses a blood hemolysis detection kit and a detection method, and the kit comprises a hemoglobin cracking reagent which is used for destroying hemoglobin to release internal iron ions and / or ferrous ions; and the iron ion fluorescent probe is used for detecting the iron ion concentration in combination with fluorescence detection. The invention provides a strategy different from conventional hemolysis detection: the hemolysis degree is indirectly judged by detecting the content of Fe, specifically, the erythrocyte releases free hemoglobin after hemolysis, and the hemoglobin contains specific iron; based on the principle, hemoglobin is destroyed and cracked to release internal Fe and / or Fe, then a self-made iron ion fluorescent probe is adopted, and a fluorescence measurement means is combined, so that the iron ion concentration can be detected, and the hemolysis degree of the blood to be detected is judged, namely the higher the iron ion concentration is, the more serious the hemolysis is.

A stool resuspension comprising a hemoglobin stabilizing reagent is provided.SOLUTION: In some embodiments, the hemoglobin stabilizing reagent may be an osmolyte, a multivalent cation, a sugar or polysaccharide, and optionally a multivalent cation, a protoporphyrin, or an HRP stabilizing component, and optionally a multivalent cation. Also provided are methods of stabilizing hemoglobin in a stool sample in a solution, and sample collection devices comprising the solution.SELECTED DRAWING: None

The invention relates to a hemoglobin oxygen carrier based on small molecule modification as well as a preparation method and application thereof. The hemoglobin oxygen carrier comprises hemoglobin and N-fluoromethoxycarbonyl diphenylalanine, the hemoglobin forms the hemoglobin oxygen-carrying carrier through the non-covalent bond acting force between the hemoglobin and the N-fluoromethoxycarbonyl diphenylalanine. According to the hemoglobin oxygen carrier disclosed by the invention, a co-assembly strategy of N-fluoromethoxycarbonyl diphenylalanine and hemoglobin is rapidly implemented by taking a non-covalent bond acting force as a driving force. The hemoglobin oxygen-carrying carrier prepared by the process greatly shortens the stabilization time of hemoglobin molecules on the basis of ensuring the stability of molecular chemical groups, has good biocompatibility and injectable application characteristics on the basis of the characteristics of non-covalent acting force, and provides a new foundation for research and development of a new generation of hemoglobin oxygen-carrying carriers. And an engineering design thought and a theoretical basis are provided.

To provide a method for increasing a shelf-life of a preserved red blood cell composition for drug delivery.SOLUTION: Provided is a method for increasing the shelf-life of a preserved red blood cell composition for drug delivery, comprising: obtaining a red blood cell comprising a pharmaceutical agent; treating the red blood cell comprising the pharmaceutical agent with a chemical agent to prepare a red blood cell comprising a hemoglobin derivative; and storing the red blood cell comprising the hemoglobin derivative under a storage atmosphere comprising an oxygen pressure of less than 20 millimeters of mercury (mmHg) to prepare a preserved red blood cell composition for drug delivery.SELECTED DRAWING: Figure 1

The invention provides a hemolysis index quality control product as well as a preparation method and application thereof. The hemolysis index quality control product provided by the invention comprises serum, a preservative and a hemolysis component, the serum, the preservative and the hemolysis component are mixed in advance according to different preset proportions to obtain quality control products with different hemolysis indexes; the hemolysis component is a blood sample with damaged red blood cells or a hemoglobin standard substance; the preservative is 0.05% of sodium azide. The hemolysis index quality control product provided by the invention can be used for monitoring the reaction and detection capability of a clinical laboratory inspection instrument on a hemolysis sample, and can also be used for assisting biochemical detection, analysis and judgment.

The application discloses a hemoglobin mutant with improved stability and application thereof, and belongs to the technical field of genetic engineering. The application lays a foundation for obtaining an ideal stable human hemoglobin mutant by designing a high-throughput screening method for the structural stability of human hemoglobin and the hematin binding intensification mutant. The application determines hot amino acid sites for the structural stability and thermal stability modification of human hemoglobin through semi-rational design, and further carries out iterative saturation mutation to obtain an ideal mutant rHb 9th The application has the advantages that in terms of structural stability, thermal stability, anti-autoxidation capacity, hematin binding rate, anti-hematin loss rate and human hemoglobin yield, etc. wild‑type The application has the advantages that in terms of structural stability, thermal stability, anti-autoxidation capacity, hematin binding rate, anti-hematin loss rate and human hemoglobin yield, etc.

This application relates to the field of medical image processing technology, specifically to a hemoglobin emphasis device and method for endoscopes. The device includes a light source unit; an imaging unit for acquiring reflected light signals from a target area under multispectral illumination and outputting a raw Bayer format image or an RGB color image; and an image processing unit for receiving and processing the output Bayer format image or RGB color image, comprising at least: a spectral reconstruction module for calculating and reconstructing a single-band image signal using a spectral reconstruction model; a hemoglobin concentration calculation module configured to calculate a relative hemoglobin concentration based on the image signal; and an image enhancement module configured to perform pixel-level gain adjustment on the raw color image or the processed image, convert it to a set color space for contrast mapping, and finally output a hemoglobin-emphasized image. The image output by this invention can significantly improve the contrast between blood vessels and surrounding tissues.

Apparatus and methods for a blood sample (48, 50) are described that include measuring a hemoglobin concentration of at least a portion (48) of the blood sample by performing a first measurement on the blood sample. The mean corpuscular hemoglobin of the blood sample (48, 50) is measured by performing a second measurement on the blood sample (48, 50). A parameter of the blood sample is determined based on a relationship between the hemoglobin concentration and the mean corpuscular hemoglobin. Other applications are described.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

An object of the present invention is to provide an eluent for suppressing bimodality of hemoglobin F which is a β - thalassemia disease marker in hemoglobin analysis in cation exchange chromatography and stably separating hemoglobin F without depending on the type of diluent, and a method for separating hemoglobin F and a method for diagnosing hemoglobin F using the eluent.SOLUTION: In the analysis of a hemoglobin sample using cation exchange chromatography, the problem is solved by an eluent having a pH of 5.0 to 6.0, which is passed when separating and quantifying hemoglobin F from the hemoglobin sample, wherein the eluent contains at least one or more pH buffers, and a relationship between the pH of the eluent and a pKa value in a range of 5.0 to 7.0 among pKa values indicated by the pH buffers is a relationship of pH ≤ pKa ≤ pH + 1.0.SELECTED DRAWING: Figure 1

The invention provides a color correction method and apparatus, an electronic device and a computer readable storage medium. The method comprises the steps of obtaining a target face image; determining a melanin dominant component and a hemoglobin dominant component in the target face image; determining a skin color type corresponding to the target face image according to the melanin dominant component and the hemoglobin dominant component; determining a correction parameter corresponding to the skin color type; correcting a preset color correction matrix through the correction parameter to obtain a target color correction matrix; and correcting the target face image through the target color correction matrix. The melanin dominant component and the hemoglobin dominant component in the target face image are determined, so that the skin color features of the user in the target face image are determined, the specific skin color type is determined, the color correction matrix is adjusted according to the skin color type, and then correction is performed through the target color correction matrix, so that the color correction accuracy is improved. And different skin color types can be adapted.

The invention relates to the technical field of composite films, in particular to a preparation process of a ZIF-8 / Hb mixed LB film, which comprises the following steps: adding a hemoglobin solution into an aqueous solution of zinc nitrate hexahydrate, and stirring for reaction to obtain a first reaction solution; adding an aqueous solution of 2-methylimidazole into the first reaction solution, carrying out a stirring reaction, carrying out centrifugal washing, and drying to obtain ZIF-8 / Hb nanoparticles; adding the ZIF-8 / Hb nanoparticles into an isooctane solution of sodium dioctyl sulfosuccinate, and carrying out ultrasonic dispersion to obtain a second reaction solution; drawing a pi-A curve of ZIF-8 / Hb, and preparing a ZIF-8 / Hb film through a multifunctional LB film drawing machine and setting film drawing parameters; according to the invention, a reverse micelle synergistic stable interface is realized, a nano-particle film forming bottleneck is broken through, the biological activity is accurately reserved, an electrochemical sensing interface and technological parameters are intelligently regulated and controlled, and the effect of customizable film performance is realized.

According to the real-time bleeding monitoring method and device based on the spectrometry, the equipment and the storage medium, the drainage container is clamped through the intelligent detection clamp, the instantaneous flow and the corresponding actual absorbance data of the drainage container under the reference wavelength and the multiple different specified wavelengths are collected in real time, and collection is convenient and fast; according to all actual absorbance data and the hemoglobin concentration quantitative analysis model, the current hemoglobin concentration is determined objectively, accurately and efficiently; according to the current hemoglobin concentration and the instantaneous flow, the hemoglobin loss rate and the total hemoglobin loss amount are determined in real time, according to the hemoglobin loss rate and the total hemoglobin loss amount, first grading early warning is carried out, and systolic pressure and heart rate are collected in real time; according to the hemoglobin loss total amount, the systolic pressure and the heart rate, the second grading early warning is carried out, and real-time precise double grading early warning is convenient for related personnel to timely and accurately evaluate the bleeding condition and take corresponding measures.

Systems and methods for detecting cognitive disorders are disclosed. The head-mounted sensor may be worn by a user. The head-mounted sensor includes an optical sensor for collecting an optical response from a region of interest in the user's brain. The user input interface receives a user test input from a test device for performing a stimulation test on a user. The controller is coupled to the sensor device and the user input interface. A controller collects optical response data from the optical sensor and test input data from the test device. The controller determines a hemoglobin level in the region of interest from the optical response data. The determined hemoglobin level in the region of interest is associated with a user test input in response to the stimulation test. Brain activity is evaluated based on the determined hemoglobin level.

The application relates to a sample analyzer and a sample analysis method. The sample analyzer comprises: a sample preparation device configured to prepare a pretreatment test solution by using a blood sample and a pretreatment reagent, prepare an interferent detection test solution by using the pretreatment test solution and a first detection reagent, and prepare a to-be-tested sample solution by using the pretreatment test solution or the interferent detection test solution and a second detection reagent; a first detection device configured to detect the interferent detection test solution to obtain interferent information; a second detection device configured to detect the to-be-tested sample solution to obtain a hemoglobin detection result and / or a glycated hemoglobin detection result of the blood sample; and a controller configured to output prompt information when the interferent information indicates that there is an interferent in the blood sample which interferes with the hemoglobin detection and / or the glycated hemoglobin detection. The embodiments of the application can detect the interferent and output corresponding interferent prompt information when the hemoglobin and / or the glycated hemoglobin is detected.

The present invention relates to a hemoglobin protein composition comprising biologically active hemoglobin and less than 2% methemoglobin (metHb) wherein the metHb is maintained below 2% after reoxygenation of hemoglobin. Furthermore, hemoglobin protein composition comprising biologically active hemoglobin and less than 25% of charge variants of total oxygenated hemoglobin. Moreover, the present invention also provides an effective concentration of antioxidant more than 5 mM used during heat treatment. The present invention provides pharmaceutically stable composition of hemoglobin. Furthermore, the present invention also provides a process for reducing and / or controlling the formation of metHb, charge variants and inactivation of viral and / or prion during manufacturing of oxygenated hemoglobin. In addition, the invention provides therapeutic use of oxygenated hemoglobin.

A hemoglobin peptide and preparation method thereof that can promote iron absorption, the mass content of polypeptide in the hemoglobin peptide is greater than 90%, containing at least one of FERF and FDGL;Preparation method includes: S1, after adding trisodium citrate to fresh animal blood, centrifugation adds NaCl solution to wash, after preparing solution, high pressure homogenization broken wall 2 5 times, obtain hemoglobin solution;S2, after adding 10 200 U / mL neutral protease heat treatment, then add 5 50 U / mL flavor protease enzymolysis, deactivate enzyme to obtain enzymolysis solution;S3, ultrafiltration membrane filtering, collect permeate;S4, after adjusting pH, add in anion exchange chromatography column, NaCl solution elution, collect eluate;S5, desalination, concentration, obtain hemoglobin peptide after drying;S6, mass spectrometry detection, bioactive peptide screening, obtain short peptide with promoting iron absorption activity. The hemoglobin peptide obtained by the preparation method of the present invention has good iron chelating ability and promotes iron absorption ability, and can significantly improve the bioavailability of iron ions.

An immunological measurement method for hemoglobin, comprising a step of performing the immunological measurement using an anti-hemoglobin antibody under coexistence of the hemoglobin and haptoglobin, the haptoglobin having a characteristic (1) below: (1) at least a portion of an N-linked glycan is deleted. The present invention further provides a method for suppressing change in measurement values of hemoglobin, a method and a solution for stabilizing hemoglobin, a hemoglobin measurement kit, and the haptoglobin and a method for producing the same. According to the present invention, hemoglobin can be measured while suppressing haptoglobin-associated change in hemoglobin measurement values.