150 results about "A hemoglobin" patented technology

The invention discloses a hemoglobin-resistant Taq DNA polymerase mutant and a construction method thereof, and relates to the field of biology, and the hemoglobin-resistant Taq DNA polymerase mutant is characterized in that a nucleotide sequence for coding the Taq DNA polymerase is shown as SEQ ID NO.1, and the mutant comprises at least one mutation or all mutations selected from S623D and E721A sites. On the basis of natural Taq DNA polymerase, the molecular structure of the Taq DNA polymerase is modified through rational design and combination with a site-specific mutagenesis biotechnology, so that the Taq DNA polymerase mutant is more suitable for PCR amplification of samples containing hemoglobin and the like than a wild type, and the result judgment accuracy of a sample containing a hemoglobin inhibitor is more facilitated.

The invention belongs to the field of biological medicine, and provides a protection liquid capable of preserving in-vitro animal organs or in-vitro animal tissues at normal temperature as well as a preparation method and application of the protection liquid, and the protection liquid comprises a solution containing a hemoglobin oxygen carrier or a hemoglobin carrier, an osmotic pressure regulating substance and the like. The protective liquid provided by the invention can be used for preserving or perfusing broken limbs, isolated animal organs or isolated animal tissues at normal temperature, and can also be used as a culture liquid of artificial organs and artificial tissues.

The invention belongs to the technical field of medical treatment, and particularly relates to a hemoglobin chyle joint detection device and a detection method.The hemoglobin chyle joint detection device comprises a shell, an analysis mechanism is arranged in the shell, and a colorimetric tube is inserted into the upper surface of the shell; the analysis mechanism comprises a light source module, a sample processing module, an optical detection module and a data processing module; the optical detection module synchronously acquires absorbance signals under two wavelengths through double detectors; the data processing module calculates hemoglobin concentration and chyle degree based on the dual wavelength absorbance values. The method is different from traditional single-index detection, two-parameter synchronous analysis of hemoglobin and chyle is achieved for the first time, and the preliminary screening efficiency before blood donation is remarkably improved. By adopting a dual-wavelength correction algorithm, the influence of residual absorption interference of hemoglobin at 580-700nm on chyle index determination is eliminated, and the influence of absorption interference of chyle at 480-570nm on hemoglobin determination is eliminated. The method is suitable for rapid screening on a blood sampling site to realize informatization management of detection data.

A stool resuspension solution comprising a hemoglobin stabilization reagent is provided. In some embodiments, the hemoglobin stabilization reagent may be an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, or an HRP stabilization component and, optionally, a polyvalent cation. A method of stabilizing hemoglobin in a stool sample in the solution is also provided, as well as a sample collection device containing the solution.

The invention relates to a continuous noninvasive physiological parameter detection method and device, and belongs to the technical field of physiological parameter detection. According to the method, on the basis of photoelectric volume pulse wave detection data under multiple wavelengths, absorbance related characteristic parameters and pulse wave related characteristic parameters are extracted, a hemoglobin concentration detection result is obtained through calculation of a hemoglobin concentration calculation model, and the absorbance related characteristic parameters comprise the adjacent surface difference, the relative difference, the adjacent surface ratio, the relative ratio and the progressive surface ratio. Extracting based on a normalized absorbance fluctuation amplitude curve; the pulse wave characteristic parameters comprise rising branch maximum slope, rising time, falling time, dicrotic wave slope, dicrotic amplitude ratio, rising branch time ratio, low-high frequency energy ratio and harmonic component ratio, and extraction is carried out based on normalized pulse waves. The detection device for implementing the method comprises a probe, a data processing circuit and the like. The accuracy of the noninvasive hemoglobin concentration detection result can be effectively improved.

The invention relates to a hemoglobin peptide capable of promoting iron absorption and a preparation method thereof, the mass content of polypeptide in the hemoglobin peptide is greater than 90%, and the hemoglobin peptide contains at least one of FERF and FDGL; the preparation method comprises the following steps: S1, adding trisodium citrate into fresh animal blood, centrifuging, adding a NaCl solution for washing, preparing the solution, and performing high-pressure homogenization wall breaking for 2-5 times to obtain a hemoglobin solution; s2, adding 10-200 U / mL of neutral protease, carrying out heat preservation treatment, then adding 5-50 U / mL of flavourzyme, carrying out enzymolysis, and carrying out enzyme deactivation to obtain an enzymatic hydrolysate; s3, filtering with an ultrafiltration membrane, and collecting permeate; s4, adjusting the pH value, adding into an anion exchange chromatographic column, eluting with a NaCl solution, and collecting the eluent; s5, desalting, concentrating and drying to obtain hemoglobin peptide; and S6, carrying out mass spectrometric detection, and screening bioactive peptides to obtain the oligopeptide with the activity of promoting iron absorption. The hemoglobin peptide obtained by the preparation method disclosed by the invention has relatively good iron chelation capability and iron absorption promoting capability, and the bioavailability of iron ions can be remarkably improved.

A method of producing a solution containing hemoglobin A2 and a method of producing a hemoglobin precision control substance, the methods including contacting a solution containing hemoglobin and having a pH of from 7.8 to 9.5 with an anion exchanger, are provided.

The invention relates to the technical field of chromatographic detection, and particularly discloses a fluorescent microsphere labeled fecal occult blood quantitative reagent strip and a preparation process thereof. The fluorescent microsphere labeled fecal occult blood quantitative reagent strip comprises a fluorescent microsphere labeled solution, and is prepared by the following steps: firstly taking fluorescent microspheres, adding an activating solution for activation, then adding a coupling solution and a hemoglobin antibody I for coupling, then adding a chicken IgY antibody for continuous coupling, and then adding a preservation solution to obtain the fluorescent microsphere labeled solution; wherein the preserving fluid is prepared from a tris (hydroxymethyl) aminomethane hydrochloride buffer solution, trehalose, bovine serum albumin, gleditsia sinensis lam gum and methionine. According to the fluorescent microsphere-labeled fecal occult blood quantitative reagent strip disclosed by the invention, the hemoglobin antibody I and the chicken IgY antibody are labeled one by one through the fluorescent microspheres by virtue of a linked labeling process, the detection range is expanded, the detection sensitivity and specificity are improved, and the stability of the reagent strip is improved by virtue of a specific preservation solution.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

The utility model relates to the technical field of livestock and poultry blood utilization, in particular to a hemoglobin low-temperature extraction device which adopts two-stage separation and integrated design, is high in integration level, suitable for small-scale hemoglobin extraction work and good in practicability. Comprising a tank; the storage crushing tank is installed on the upper portion of the tank body, the liquid inlet pipe is installed on the top of the storage crushing tank, the first centrifugal separation tank is installed in the middle of the tank body, the storage crushing tank is communicated with the first centrifugal separation tank through a pipeline, and the second centrifugal separation tank is installed in the middle of the storage crushing tank through a pipeline. A residue discharging pipe is installed on the side wall of the first centrifugal separation tank, the upper end of the conveying pipe is communicated with the first centrifugal separation tank, the second centrifugal separation tank is installed on the lower portion of the tank body, a first liquid discharging pipe is installed on the side wall of the second centrifugal separation tank, and the upper end of the second liquid discharging pipe is communicated with the bottom of a second separation cavity of the second centrifugal separation tank. The upper end of the second liquid discharging pipe is located on the inner side of the inner end of the first liquid discharging pipe.

To provide a color and shape change monitoring system capable of accurately monitoring the color and shape change of a human body caused by a blood flow change.SOLUTION: The image processing apparatus includes a three dimensional point cloud data acquisition unit configured to acquire three dimensional point cloud data of a measurement target portion of a human body, a color data acquisition unit configured to acquire color data of the measurement target portion, a color-shape-data generation unit configured to generate color-shape data of the measurement target portion in which the color data is associated with the three dimensional point cloud data, and a pigment component separation unit configured to separate the color-shape data into a hemoglobin pigment component, a melanin pigment component, and a shade component.SELECTED DRAWING: Figure 1

The present application relates to a non-invasive hemoglobin detection method based on hyperspectral image reconstruction, belonging to the field of physiological signal detection. The present application irradiates human skin tissue by using a visible light source, simultaneously uses a hyperspectral camera and an RGB camera to simultaneously collect images of the irradiated skin area as a training set, trains a deep learning model, and realizes hyperspectral reconstruction of ordinary RGB images. A hemoglobin extraction feature extraction model for the visible light range diffuse reflectance spectrum is established using an algorithm, and the real hemoglobin content of the measured human body is output through a hemoglobin correction model.

The application discloses a hemoglobin camel-derived nanobody and a preparation method and application thereof. The hemoglobin camel-derived nanobody is an amino acid sequence shown in SEQ ID NO: 7, is a hemoglobin camel-derived nanobody specific to hemoglobin derived from a bactrian camel, has small molecules, can easily penetrate, has high specificity, good stability, is easy to produce and transform, has wide application prospects, and can be applied to the fields of biomedical research, clinical diagnosis and treatment and the like.

To provide dosing regimens for administering a HIF prolyl hydroxylase inhibitor in the treatment or prevention of anemia, such as anemia secondary to or associated with chronic kidney disease, or anemia associated with or resulting from chemotherapy.SOLUTION: A pharmaceutical composition in a form of a tablet or capsule comprising Compound 1 having the following structure, for use in a method of increasing hemoglobin levels in a patient in need thereof relative to a baseline hemoglobin level, wherein the patient has a hemoglobin level of ≤10.5 g / dL or ≥9.5 g / dL and ≤12 g / dL at a start of treatment, a serum hemoglobin level increases by at least about 0.1 to about 1.0 g / dL relative to the baseline hemoglobin level, and the pharmaceutical composition is used such that Compound 1 is administered to the patient once daily, for example at a dose of about 150 mg.SELECTED DRAWING: Figure 1a

A recombinant hemoglobin, a nucleic acid encoding the recombinant hemoglobin, and a preparation method therefor are provided to address the current problems of complex processes, high production costs, and low yields that exist for improving a hemoglobin having a stable tetrameric structure. The recombinant hemoglobin comprises: two or more units of recombinant hemoglobins, the units of recombinant hemoglobins each comprise four polypeptide chains, and the polypeptide chains are two α chains and two β chains. The amino acid sequences of the four polypeptide chains of each unit of recombinant hemoglobin are linked by means of intra-unit linker peptides to form the unit of recombinant hemoglobin, and two or more of the units are linked by means of inter-unit linker peptides.

Disclosed are an apparatus and method for estimating a target component value. The apparatus for estimating a target component value may include: a sensor configured to obtain a spectrum of light scattered or reflected from an object; and a processor configured to correct a first reflectance value of the spectrum based on a melanin index; obtain a second reflectance value based on the correction of the first reflectance value; convert the second reflectance value into an absorbance value; estimate the target component value based on the absorbance value; and correct the target component value based on a hemoglobin index.

This invention relates to the field of blood testing technology and proposes a hemoglobin detection and analysis instrument, including a detection platform and a detection box and a machine set on the detection platform. The top surface of the detection platform is provided with an electric guide rail that can drive the machine to move in and out of the detection box. A drive unit is installed on the inner side of the machine. A rotating arm fixed to the output end of the drive unit is provided above the machine. Two sets of carriers arranged in a straight line are provided on the rotating arm. In this invention, because the two magnetic poles at the outer ends of the two magnetic columns of one telescopic component in the same group are opposite, while the two magnetic poles at the outer ends of the two magnetic columns of the other telescopic component in the same group are the same, and the gear transmission ratio of the two carriers in the same group is 1:1, during the rotation of the two carriers in the same group, through the magnetic attraction and repulsion of the two telescopic components, the sample tube can be made to swing back and forth under the clamping component, which further increases the mixing effect compared with the prior art.

The invention discloses a method for removing methemoglobin in a hemoglobin solution. The method comprises the following steps: firstly, adjusting the pH value of the hemoglobin solution to 6.5-8.5 and the ion strength to 0-3000mmol / L; deoxidizing the hemoglobin solution to obtain a deoxidized hemoglobin solution; raising the temperature of the deoxidized hemoglobin solution to a certain temperature and maintaining the temperature for a certain time; and finally, removing suspended or precipitated substances in the solution to obtain the hemoglobin solution for removing the methemoglobin. The process steps are pure physical processes, and the MetHb component can be effectively removed on the premise that new impurities or new components are not brought into an original system; the hemoglobin structure and oxygen carrying and releasing performance are not obviously influenced, the technological process is simpler than that of a chemical method, and the cost is low. In addition, the treatment process is easy to control, the content of Hb in the solution is not affected by the process, and the property is stable. The whole adopted temperature range is similar to the temperature condition of the existing biological product pasteurization virus inactivation process, and the method has the possibility of being combined with the virus inactivation process and has good application value.

The application relates to a biochemical analyzer and a detection method for detecting glycated hemoglobin. The hemoglobin concentration and the glycated hemoglobin concentration of a to-be-detected whole blood sample are obtained, and the content proportion of the glycated hemoglobin is calculated according to the hemoglobin concentration and the glycated hemoglobin concentration. The hemoglobin concentration is obtained by the following steps: obtaining a first absorbance of a to-be-detected sample liquid at a hemoglobin measurement wavelength, wherein the to-be-detected sample liquid is prepared by mixing a to-be-detected whole blood sample, a hemolytic agent and a hemoglobin detection reagent; obtaining at least two second absorbances of the to-be-detected sample liquid at at least two different correction wavelengths, wherein the correction wavelengths are designed so that the absorbance of a lipid interferent at the correction wavelength is greater than the absorbance of hemoglobin at the correction wavelength; correcting the first absorbance according to the second absorbance; and determining the hemoglobin concentration according to the corrected first absorbance. The application can improve the accuracy of hemoglobin detection.

The system comprises a laminated 5 diagnostic strip including a backing layer (105), an absorbent pad layer (106) configured for controlled capillary transport of milk, and a reagent deposit layer (107) defining multiple spatially separated dry reagent zones. The reagent zones include a pH indicator zone (101), a leukocyte esterase detection zone (102), a hemoglobin detection zone (103), and a nitrite detection zone (104), each adapted 10 for operation in the milk matrix. A calibrated sample application point (108) receives a predefined volume of untreated milk and fluidically communicates the sample to the reagent zones via the absorbent pad layer. Colorimetric reactions develop in a result window area (109), enabling simultaneous visual interpretation without analytical instruments, typically within three minutes. The invention further 15 includes a moisture-controlled storage container comprising desiccant elements (204, 205), a reference color chart (208), and a calibrated pipette (209), ensuring reagent stability and standardized use.

A blood analyzer and a hemoglobin detection method, the method comprising: a control device controls a sampling device (11) to obtain a sample to be tested (201); a control device controls a sample preparation device (12) to mix a portion of the sample to be tested with a first reagent to prepare a first sample liquid to be tested, and a control device controls a portion of the sample to be tested with a second reagent to prepare a second sample liquid to be tested (202); a control device controls a colorimetric detection device (13) to perform a hemoglobin detection on the first sample liquid to be tested using a colorimetric method to obtain a first measurement value (203); a control device controls an optical detection device (14) to perform a hemoglobin detection on the second sample liquid to be tested using a light scattering method to obtain a second measurement value (204); and a control device determines whether the sample to be tested is a sample with a falsely high hemoglobin level (205); if the sample to be tested is a sample with a falsely high hemoglobin level, the control device outputs a second measurement value (206); if the sample to be tested is a sample with a falsely high hemoglobin level, the control device outputs a first measurement value (207).

The invention relates to the technical field of biological detection, in particular to a hemoglobin content prediction method and equipment, and the method comprises the following steps: processing multiple groups of light absorption signals obtained based on a hemoglobin solution to obtain time sequence data; inputting the time sequence data into a one-dimensional convolutional neural network to extract multi-scale local features, and inputting the multi-scale local features into an attention transformation network to extract global information and local information to obtain comprehensive attention features; extracting a relationship between different peaks in the target signal corresponding to the time sequence data through a graph attention network to obtain a peak-to-peak relationship feature; and inputting the comprehensive attention features and the peak-to-peak relationship features into a feature fusion network for fusion, and calculating a hemoglobin content index based on the obtained fusion features. The method comprehensively considers the influence of the overall and local characteristics, the peak-to-peak relationship and the like, so that the hemoglobin content index can be accurately detected.

The invention comprises a process and composition relating to the use of an artificial blood that is a pharmaceutically acceptable solution of an iron amino acid chelate. The composition is directed to a hemoglobin free artificial blood composition made from one or more amino acids known to be bound to the hemoglobin molecule but free of hemoglobin for purposes of this invention. The hemoglobin free amino acids are chelated with iron [ferrous or ferric], oxygenated, and used as an artificial blood replacement for whole blood or as a component of an artificial blood composition. The iron component of the amino acid chelate is oxygenated to enable the chelate to carry oxygen throughout the body. To form a solution suitable for transfusion, the oxygen bearing iron chelate of the amino acid is dissolved, to the extent necessary, preferably in an isotonic saline solution. In use, the hemoglobin free artificial blood composition is transfused into an individual suffering trauma due to loss of blood using conventional transfusion procedures know in the art.

In at least one embodiment, a hemoglobin measuring device may include a plurality of sensors, a display configured to present information, and a computing device in communication with the sensors and display and having a processor and a memory storing instructions executable by the processor. The instructions may include instructions to receive, from the sensors, measurements of one or more forms of glycated hemoglobin in a blood samples, determine a total fetal hemoglobin value in the blood sample by performing a peak area summation of all forms of fetal hemoglobin, subtract the total fetal hemoglobin value from 100 to derive a total adult hemoglobin value, determine a glycated fetal hemoglobin value, compare the glycated fetal hemoglobin value to a reference range to obtain a comparison, determine a baseline glucose control value based on the comparison, and output the baseline glucose control value and the comparison to a display for medical evaluation.

The invention discloses a blood hemolysis detection kit and a detection method, and the kit comprises a hemoglobin cracking reagent which is used for destroying hemoglobin to release internal iron ions and / or ferrous ions; and the iron ion fluorescent probe is used for detecting the iron ion concentration in combination with fluorescence detection. The invention provides a strategy different from conventional hemolysis detection: the hemolysis degree is indirectly judged by detecting the content of Fe, specifically, the erythrocyte releases free hemoglobin after hemolysis, and the hemoglobin contains specific iron; based on the principle, hemoglobin is destroyed and cracked to release internal Fe and / or Fe, then a self-made iron ion fluorescent probe is adopted, and a fluorescence measurement means is combined, so that the iron ion concentration can be detected, and the hemolysis degree of the blood to be detected is judged, namely the higher the iron ion concentration is, the more serious the hemolysis is.

A stool resuspension comprising a hemoglobin stabilizing reagent is provided.SOLUTION: In some embodiments, the hemoglobin stabilizing reagent may be an osmolyte, a multivalent cation, a sugar or polysaccharide, and optionally a multivalent cation, a protoporphyrin, or an HRP stabilizing component, and optionally a multivalent cation. Also provided are methods of stabilizing hemoglobin in a stool sample in a solution, and sample collection devices comprising the solution.SELECTED DRAWING: None

The invention relates to a hemoglobin oxygen carrier based on small molecule modification as well as a preparation method and application thereof. The hemoglobin oxygen carrier comprises hemoglobin and N-fluoromethoxycarbonyl diphenylalanine, the hemoglobin forms the hemoglobin oxygen-carrying carrier through the non-covalent bond acting force between the hemoglobin and the N-fluoromethoxycarbonyl diphenylalanine. According to the hemoglobin oxygen carrier disclosed by the invention, a co-assembly strategy of N-fluoromethoxycarbonyl diphenylalanine and hemoglobin is rapidly implemented by taking a non-covalent bond acting force as a driving force. The hemoglobin oxygen-carrying carrier prepared by the process greatly shortens the stabilization time of hemoglobin molecules on the basis of ensuring the stability of molecular chemical groups, has good biocompatibility and injectable application characteristics on the basis of the characteristics of non-covalent acting force, and provides a new foundation for research and development of a new generation of hemoglobin oxygen-carrying carriers. And an engineering design thought and a theoretical basis are provided.

To provide a method for increasing a shelf-life of a preserved red blood cell composition for drug delivery.SOLUTION: Provided is a method for increasing the shelf-life of a preserved red blood cell composition for drug delivery, comprising: obtaining a red blood cell comprising a pharmaceutical agent; treating the red blood cell comprising the pharmaceutical agent with a chemical agent to prepare a red blood cell comprising a hemoglobin derivative; and storing the red blood cell comprising the hemoglobin derivative under a storage atmosphere comprising an oxygen pressure of less than 20 millimeters of mercury (mmHg) to prepare a preserved red blood cell composition for drug delivery.SELECTED DRAWING: Figure 1

The invention provides a hemolysis index quality control product as well as a preparation method and application thereof. The hemolysis index quality control product provided by the invention comprises serum, a preservative and a hemolysis component, the serum, the preservative and the hemolysis component are mixed in advance according to different preset proportions to obtain quality control products with different hemolysis indexes; the hemolysis component is a blood sample with damaged red blood cells or a hemoglobin standard substance; the preservative is 0.05% of sodium azide. The hemolysis index quality control product provided by the invention can be used for monitoring the reaction and detection capability of a clinical laboratory inspection instrument on a hemolysis sample, and can also be used for assisting biochemical detection, analysis and judgment.

To provide a hemoglobin type identification method, learned model generation method, learned model, hemoglobin analysis system and program that can identify a type of hemoglobin in a blood sample even when using separation data in which a separation time of separation processing to a blood sample is short.SOLUTION: A hemoglobin type identification method is configured to: a prepare a learned model created by using a data set including learning-purpose frequency analysis data created by analyzing a frequency of separation data created by separation processing to a learning-purpose blood sample having a type of hemoglobin known, and the known type of the hemoglobin as a learning-purpose data set of machine learning; analyze a frequency of separation data created by separation processing to a learning-purpose blood sample having the type of hemoglobin unknown, and prepare inspection-purpose frequency analysis data; an input the inspection-purpose frequency analysis data to the learned model, and thereby identify a type of the hemoglobin included in the inspection-purpose blood sample.SELECTED DRAWING: Figure 3