158 results about "Hemolytic Agents" patented technology

The present invention relates to articles coming into contact with bodily fluids, preferably absorbent articles like sanitary napkins and pantiliners, which comprise an oxidising agent having a reduction potential higher than that of the reaction of Fe<2+> to Fe<3+> togather with a hemolytic agent. In a preferred embodiment the articles further comprise an additional odour control agent.

There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the step (3).

The invention relates to a hemolytic agent for blood leukocyte five-classification counting and an application thereof. The hemolytic agent comprises a hemolytic agent A and a hemolytic agent B. The hemolytic agent A comprises a polyoxyethylene nonionic surfactant, an organic acid, a quaternary ammonium salt cationic surfactant cosolvent, a stabilizing agent and an osmotic regulator; the hemolytic agent B comprises alkaline inorganic salt and an osmotic regulator. When compared with the result detected by a standard hemolytic agent produced by BeckmanCoulter company, the hemolytic agent of the invention has good correlation and high accuracy. Especially, when abnormal cells exist in a blood specimen, the hemolytic agent has high warning sensitivity and good specificity, and exhibits the most beneficial effect.

The invention discloses a fluid system of a flow cytometer and a flow cytometric detection method. The fluid system comprises a sampling needle, a sampling needle cleaning device, a flowing chamber, a reaction tank, a hemolytic agent syringe, a sample syringe, a diluent syringe, a diluent bottle, a hemolytic agent bottle and a motor, wherein the sample syringe is respectively communicated with the sampling needle, the reaction tank, the flowing chamber, the sampling needle cleaning device and the diluent syringe, the diluent syringe is respectively communicated with the diluent bottle, the flowing chamber and the reaction tank, the hemolytic agent syringe is respectively communicated with the hemolytic agent bottle and the reaction tank, and the hemolytic agent syringe, the sample syringe and the diluent syringe are all driven and controlled by the motor. According to the invention, driving motors for a plurality of syringes are integrated into one, arrangement of the syringes satisfies moment balance, integral rigidity and inertia are improved, gap instability of the driving motor can be buffered, a more stationary sample flow can be obtained, and thus, detection precision of the flow cytometer is improved.

The invention relates to the technical field of human blood detection. A flow cytometry detection fluid circuit system comprises a diluent injection system, a hemolysin and blood sample injection system and a pressure system, wherein for the diluent injection system, a diluent injector is driven by a motor, and the diluent injection system is connected with a diluent barrel to suck a diluent to inject to a flowing chamber, a reaction tank and a sampling needle cleaning device; for the hemolysin and blood sample injection system, a hemolytic agent injector is driven by a motor, the hemolysin and blood sample injection system is used for sucking the hemolytic agent to inject to the reaction tank, and the sample injection sucking collects a distribution blood sample to inject to the reaction tank; for the pressure system, a diaphragm pump is connected with a pressure chamber to form positive and negative pressures, the pressure system is used for pumping waste liquid of the sampling needle cleaning device, sucking a sample in the reaction tank to a sample injection port of the flowing chamber, carrying out gas blowing stirring and evacuation on the reaction tank, and pumping the diluent to the reaction tank. According to the invention, the number of reaction tanks is reduced, system structure and complexity are simplified, and miniaturization and low cost are realized for machines.

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to an agent for a blood cell analyzer. The agent for the blood cell analyzer consists of a diluent, an EOI hemolytic agent, an LH hemolytic agent, an EOII staining solution and a cleaning solution. The agent for the blood cell analyzer is wide in range of use, suitable for many types of machines, suitable for detectors with machine types of BC5100/5180/5300/5380/5310/5390 and the like, and stable and accurate in detection result; the R value of a correlation coefficient after linear regression reaches more than or equal to 0.99 compared with a Mairui original blood cell agent and expresses relatively good relativity; and moreover, the agent is rich in raw material and low in price, and the raw materials are nontoxic and harmless and do not have threats on operators, so that the burden of the environment is lightened, and the raw materials are beneficial for popularization and application of the agent for the blood cell analyzer.

The invention relates to a hemolytic agent for a blood cell analysis device. The hemolytic agent is capable of cracking the erythrocytes in the blood, and the blood with cracked erythrocytes can be used for hemoglobin concentration determination. In addition, the invention also relates to a leukocyte classification reagent system which comprises the hemolytic agent and an inhibitor. After the hemolytic agent cracks the erythrocytes, the inhibitor can be added into the blood to prevent the hemolytic agent from damaging the morphology and structure of the leucocytes. Furthermore, the invention also relates to a method of classifying the leucocytes by using the reagent system.

A liquid-based cell preservation liquid composite is characterized by comprising the following components in percentage by weight: cell fixing agent: 2-15% of formaldehyde, preservative: 0.1-5% of sodium diacetate, anticoagulant: 2-10% of sodium citrate, buffering agent: 0.5-2% of sodium chloride, chelating agent: 0.002-0.5% of ethylene diamine tetraacetic acid (EDTA), hemolytic agent: 0.5-10% ofglacial acetic acid, and water: 5-80% of pure water, wherein the formaldehyde is the analytical reagent, and accounts for 37-40%; the sodium diacetate is the food additives; the sodium citrate and the sodium chloride are analytical reagents, and NaCl is more than 99.5%; and the EDTA and the glacial acetic acid are analytical reagents, and H2COOH is more than 99.5%. The invention has the advantages that the liquid-based cell preservation liquid composite can keep the cell form to the maximum extent, can improve the diagnosis accuracy and can prevent the cell form of a specimen from significantdifference at room temperature for at least three months.

The invention discloses a fluid system of a flow cytometer and a flow cell detecting method. The fluid system of the flow cytometer comprises a sheath flow injector, a sample flow injector, a blood sampling injector, a hemolytic agent injector, a blood sampling needle, a reaction tank, a flowing chamber, a diluent barrel, a hemolytic agent bottle, a first motor, a second motor and a third motor, wherein the sheath flow injector is driven by the first motor; the hemolytic agent injector is driven by the third motor; and the sample flow injector and the blood sampling injector are driven by the second motor. The sample flow injector and the blood sampling injector share the second motor, and are arranged in a moment balancing manner; the second motor is arranged between the two injectors; the cost of the system is reduced; the whole rigidity and the inertia of the system are also improved; sample flow is pushed by the injector stably; and the detection accuracy is improved.

The invention relates to the technical field of human blood detection and discloses a flow cytometry fluid path system. The system consists of a motor drive sample stream injector and a sheath fluid injector, wherein the outlet of the sample stream injector is respectively connected with a diluent bucket and a sample injection port by virtue of a pipeline and a three-way solenoid valve and is also connected with a reaction tank; the outlet of the sheath fluid injector respectively passes through a two-way solenoid valve by virtue of a pipeline and a liquid pressure sensor and is connected with a flow chamber sample injection port, a flow chamber sheath fluid injection port and a fluid suction port of the sample stream injector; a hemolytic agent injector is driven by the motor, and the outlet of the hemolytic agent injector is respectively connected with a hemolytic agent bottle and the reaction tank by virtue of a pipeline and a three-way solenoid valve; the outlet of a sampling injector is connected with a sampling needle by virtue of a pipeline; and a diluent injection port in the sampling injector is connected with the diluent bucket by virtue of a pipeline. The system disclosed by the invention is simple in structure, low in cost and simple and reliable in detection method, the detection data is accurate, and machine miniaturization can be realized.

The invention discloses a simple and reliable method, system and device for five-classification analysis of blood cells, including a white blood cell counting pool, a red blood cell and platelet counting pool, a five-classification detection device, a hemoglobin detection device, and a red blood cell detection device. The device divides blood samples and corresponding reagents into the two counting pools for reaction. It is characterized in that the system is equipped with two kinds of hemolysis agents, namely the first hemolysis agent and the second hemolysis agent, and the white blood cell four-category count and basophil The count is measured twice by the five-differential detection device, and the four-differential count and the total white blood cell count are realized by mixing the first hemolytic agent with the blood sample; the basophilic count is realized by mixing the second hemolytic agent with the blood sample added with diluent cell counts. The invention can realize low cost and small volume, and the accuracy of basophils can reach the level of high-end five-differentiation blood cell analyzer, which is more suitable for use in primary hospitals.

The present invention relates to the field of medical immunology, particularly to an immunoturbidimetric kit for detecting procalcitonin. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer liquid, a stabilizer, a protection agent, a coagulant, a hemolytic agent and a preservative, the reagent R2 comprises a buffer liquid, a stabilizer, a protection agent, a preservative and a polystyrene latex microsphere-procalcitonin antibody complex, and the polystyrene latex microspheres comprise two types of microspheres such as microspheres having a particle size of 80-150 nm and microspheres having a particle size of 150-400 nm. According to the present invention, the test results show that the kit has advantages of good stability and long storage period, can directly detect the PCT in whole blood, and further has advantages of high detection result accuracy, high sensitivity and wide linear detection range.

A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic acid, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pallet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.

The invention discloses a leukocyte classification and a classification method thereof, and the kit is composed of a water soluble quantum dots-cyanine dye probe, and an erythrocyte and platelet hemolytic agent, wherein the quantum dots-cyanine dye probe is composed of a stabilizing agent, which takes a polypeptides material as the quantum dots, and Cy5 cyanine dye covalent joint molecules whose emission spectrum is in the near infrared area, and has the characteristics of high quantum yield and sensitivity; the erythrocyte and platelet hemolytic agent can rapidly solves erythrocytes in the blood, and is composed of a cationic surfactant, a non-ionic surfactant, a pH buffer agent or combination of the cationic surfactant, the non-ionic surfactant, and the pH buffer agent. The leukocyte classification reagent provided by the invention can carry out precise subgroup identification on lymphocyte, monocyte, neutrophil, basophilic granulocyte, and acidophilic granulocyte in leukocytes of blood samples, and further identify immature cells or abnormal cells.

The invention discloses a hemolytic agent which contains at least one organic acid whose content ranges from 1 to 20g/L, and relates to a multipurpose hemolytic agent, which can be used for automatic hemanalysis, and can accurately test hematoglobin and keep the normal classification of leucocyte at the same time. A stabilizing agent of hematoglobin derivants is added in the hemolytic agent, thereby preventing the derivants from changing along with the reaction temperature change, and ensuring that an accurate and stable test result can be obtained within the normal service temperature range of the instrument.

The invention discloses a hemolytic agent, a method for pretreatment of a biological sample, a method for determining the content of a target substance, and a kit. The invention provides a use of a boric acid buffer system in preparation of the hemolytic agent, the hemolytic agent for immunological turbidimetry determination, the method for pretreatment of the biological sample, the method for determining the content of the target substance in the biological sample, and the kit for immunological turbidimetry determination. The hemolytic agent is used for immunological turbidimetry determination. When the hemolytic agent prepared by the boric acid buffer system is used for immunological turbidimetry determination, the hemolytic agent has at least one of the following advantages of high sensitivity, fast and simple detection process and strong specificity.

A blood cell analyzer diluent and a hemolytic agent, are characterized in that one litre diluent is provided with 12.0-4.0g of sodium chloride, 2.0-10.0g of sodium sulfate, 0.8-0.5g of 1,3,2-methylol urea, 0.2-0.5g of copper sulfite, 3.0-8.0g of EDTA-2Na, 0.2-0.7g of Piperacillin Sodium, a borate buffering liquid toning the ph value to 7.2-7.8, and the balance is water; one litre hemolytic agent is provided with 0.8-5.0g of potassium chloride, 0-60.0g of dodecyl trimethyl ammonium chloride, 14.0-0g of octadecyl trimethyl ammonium bromide, 6.0-10.0ml of isopropanol, carbonate or alcaine buffering liquid toning the ph value to 7.2-7.8, and the balance is water. The inventive reagent can form stable hemoglobin derivatives, and the absorption spectrum curves are similar when lambada is 540nm, lambada is 504nm, which can satisfy the clinical inspection requirement; the reagent does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operating staff, and can reduce harm of toxicant to personal health.

The invention discloses a hemocyte analyzer hemolytic agent. Every 1L of hemolytic agent comprises 5-40.0g of quaternary ammonium salt, 5.0-25.0g of alkali metal chloride, 0.05-5.0g of ethylenediamine tetraacetic acid or ethylenediamine tetraacetic acid derivative, 0.5-5.0ml of inorganic acid, 0.5-8.0g of pH regulator for regulating the pH value to 5.0-8.0, and the balance of water. The invention solves the problem that the peak shapes of lymphocyte, monocyte and granulocyte in a leucocyte histogram of XFA6000-series and XFA6000 Intelligent-series full-automatic hemocyte analyzers produced by Nanjing Pulang Medical Equipment CO, LTD are not obvious or only the wave peaks of the lymphocyte and granulocyte can appear, and also solves a series of problems of insufficient accuracy of classification of leucocytes; and the reagent does not contain any preservative or cyanide, and thus, does not pollute the environment.

A method for improving the accuracy of leukocyte classifying result of blood sample comprises: A, in the counting process of leukocyte, adding hemolytic agent in two times, testing and obtaining a first leukocyte counting number after adding the hemolytic agent at first time and a first leukocyte histogram, and a second leukocyte counting number after adding hemolytic agent at second time, while the hemolytic dosage added in the first time makes red blood cell fragments left affecting the counting number of leukocyte in the blood sample, and the hemolytic dosage added in the second time makesno red blood cell fragments left to avoid affecting the counting number of leukocyte; B, according to the ratio between the first leukocyte counting number and the second leukocyte counting number, determining a leukocyte classification histogram, and classifying leukocytes. The method can confirm the accuracy of leukocyte counting number and hemoglobin test result, and can improve the classification accuracy of leukocyte of animal blood samples of dog, cat and the like.

The invention provides a kit for detecting serum amyloid A content and a preparation method and detection method thereof and belongs to the field of medical detection. The kit comprises a hemolytic agent R1 and a latex reagent R2 which are independent with each other. The latex reagent R2 comprises latex coated with a serum amyloid A antibody. The latex coated with the serum amyloid A antibody isprepared from latex having particle sizes of 60-130nm and coated with the anti-serum amyloid A1 antibody and latex having particle sizes of 190-220nm and coated with the anti-serum amyloid A2 antibodythrough mixing. The invention also provides a preparation method and a detection method of the kit for detecting serum amyloid A content. The kit can detect whole blood, serum, a cerebrospinal fluidand a pleuroperitoneal fluid, utilizes a small amount of samples, is free of separation or pretreatment, can be directly used for detection and has good sample compatibility.

The invention discloses a white blood cell classification reagent, which comprises the following raw materials: an ionic surfactant, a pH value adjusting buffer, an alkali metal salt for adjusting osmotic pressure, and an alcohol capable of dissolving and accelerating. According to the present invention, with the white blood cell classification reagent, white blood cells can be divided into four cell clusters, and the boundary between each cell cluster is clear; compared to the traditional five-class hemolytic agent, the white blood cell classification reagent of the present invention has thefollowing advantages that the boundary between the monocyte clusters and the neutrophil cluster is clear, and the clustering property is strong; the white blood cell classification reagent has characteristics of strong low-temperature hemolysis ability and wide temperature range, and can rapidly dissolve red blood cells in a low-temperature environment, ensure the normal classification of white blood cells, and reduce the manufacturing cost of the instrument on temperature control; and the white blood cell classification reagent further has advantages of low raw material cost, environmental protection and no toxicity, and can classify white blood cells by requiring only one reagent so as to achieve advantages of convenience, time saving and reduced production cost.

The invention provides a multifunctional support comprising a blood collecting needle, blood collecting bottle and reagent bottle support, which is used for storing blood collecting needles, blood collecting bottles and reagent bottles, wherein the blood collecting bottles can be heated and the reagent bottles can be refrigerated. A refrigeration area of the blood collecting needle, blood collecting bottle and reagent bottle support is mainly used for refrigerating reagents (including antibody reagents) requiring refrigeration, wherein an ice storage area can cool the refrigeration area on the condition of the power failure to prevent the reagents requiring refrigeration from losing efficacy at high temperature. A normal temperature area of the blood collecting needle, blood collecting bottle and reagent bottle support is used for storing reagents and solutions stored at normal temperature, such as diluents, cleaning fluid and hemolytic agents. A blood collecting needle and blood collecting bottle storage area is provided with a blood collecting needle support, a blood collecting bottle bracket, blood collecting bottle bracket guide rails, a video camera device, a limit switch and a temperature control device.

The invention discloses a blood cell and biochemical component analysis instrument and method. The analysis instrument comprises a transfer module, a first hemolytic agent adding module, a second hemolytic agent adding module, a third hemolytic agent adding module, a diluent adding module, a cleaning agent adding module, a cleaning module, an even mixing module, a control module, a first detection cup, a second detection cup, a third detection cup and a fourth detection cup. The blood cell and biochemical component analysis instrument can achieve rapid completion of conventional and biochemical item detection of blood cells in one, makes detection functions of the instrument stronger, makes the sample usage amount smaller, makes the detection efficiency higher and provides more comprehensive information for clinic disease diagnosis.

The invention belongs to the technical field of medical treatment detection equipment, and particularly relates to a hematology analyzer adopting a single negative pressure source and a pipeline flow regulator. The hematology analyzer comprises a diluent container, a hemolytic agent container, a cleaning agent container, a cleaning pond, a hollow blood sample taking needle, a needle transferring device, a photoelectric colorimetry device, a No.1 diluting colorimetric pond, a No. 2 diluting pond, a metering and measuring device, a waste liquid bottle, a constant pressure device and a negative pressure pump connected with the constant pressure device. The hematology analyzer is characterized in that the constant pressure device and the negative pressure pump are of one group to form the single negative pressure source, all devices are connected with one another through pipelines, and an electronic valve and a flow regulator are arranged on the pipeline. According to the hematology analyzer provided by the invention, the single negative pressure source is matched with a corresponding quantitative pipeline device, so that works that a blood sample, a testing reagent and waste liquid in processes of sampling, reagent injecting, uniform-mixing, detecting, discharging and the like of the hematology analyzer enter specific positions through specified channels according to specified dosage in sequence, and the like are completed, and thus an accurate detection result is ensured.