66 results about "Hemolytic Agents" patented technology

The present invention relates to the field of medical testing technology, and in particular to a method for rapidly detecting neutrophil extracellular traps. The method comprises the following steps: (1) taking a human whole blood sample, adding a hemolytic agent, and then adding a mixed fluorescent antibody staining solution, shaking and mixing, and incubating at room temperature; (2) centrifuging after incubation in step (1), discarding the supernatant, washing with a washing solution, and then centrifuging, discarding the supernatant; (3) adding a washing solution to resuspend the cells; (4) using a flow cytometer for detection. The detection method has good stability, strong repeatability, and high fluorescence intensity, and can be used for auxiliary diagnosis of diseases.

The invention discloses a compound applied to a hemolytic agent and a composition thereof, which are characterized in that a compound which has weak reducibility and can be combined with hemachrome oxygenase to limit enzymatic activity is introduced into a formula of the hemolytic agent, so that the combination ratio of saponin and hemachrome oxygenase is reduced; according to the present invention, the dependence degree of the heme-biliverdin-bilirubin oxidation reduction system on the saponin is limited, the compatibility of the hemolytic agent formula on different batches of commercial saponin products is improved, and the weak reducibility of the compound avoids the oxidation problem of the saponin on the premise of no influence on the disulfide bond of the antibody, such that the heme-biliverdin-bilirubin oxidation reduction system can be easily obtained. Finally, the effects of reducing batch-to-batch clinical deviation of the hemolytic agent and improving the long-term stability of the hemolytic agent are achieved.

A kit for quantitative analysis of C-reactive protein (CRP) according to an embodiment of the present application may comprise: a first composition comprising a hemolytic agent for hemolyzing at least a portion of blood cells in a blood sample; and a second composition comprising an anti-CRP antibody for the antigen-antibody reaction of the CRP contained in the hemolyzed sample. The CRP quantitative analysis kit of the present application can provide a stabilizer which enables a solid-phase dry anti-CRP antibody to be sufficiently redissolved to participate in an immune reaction, so that quantitative analysis of CRP can be highly accurately and accurately performed in a wide concentration range, and storage stability is improved.

The present application relates to the field of biotechnology, and specifically discloses a kit for preparing a glycated hemoglobin control product, a method for preparing a glycated hemoglobin control product, and a kit. The kit for preparing a glycated hemoglobin control product comprises: a cell washing solution comprising: a buffer, an osmotic pressure maintaining agent; a saccharification solution comprising: a buffer, an osmotic pressure maintaining agent, and glucose; a hemolytic agent comprising: a buffer, a first surfactant; and a lyophilization protectant comprising: a buffer, an osmotic pressure maintaining agent, a stabilizer, and a second surfactant. Through the above-mentioned method, the glycated hemoglobin control product has the characteristics of low cost, high accuracy, good uniformity, and strong stability. The use of the glycated hemoglobin control product can meet the purpose of quality control of the adapted glycated hemoglobin meter system, and the obtained results are accurate and reliable.

The application relates to a biochemical analyzer and a detection method for detecting glycated hemoglobin. The hemoglobin concentration and the glycated hemoglobin concentration of a to-be-detected whole blood sample are obtained, and the content proportion of the glycated hemoglobin is calculated according to the hemoglobin concentration and the glycated hemoglobin concentration. The hemoglobin concentration is obtained by the following steps: obtaining a first absorbance of a to-be-detected sample liquid at a hemoglobin measurement wavelength, wherein the to-be-detected sample liquid is prepared by mixing a to-be-detected whole blood sample, a hemolytic agent and a hemoglobin detection reagent; obtaining at least two second absorbances of the to-be-detected sample liquid at at least two different correction wavelengths, wherein the correction wavelengths are designed so that the absorbance of a lipid interferent at the correction wavelength is greater than the absorbance of hemoglobin at the correction wavelength; correcting the first absorbance according to the second absorbance; and determining the hemoglobin concentration according to the corrected first absorbance. The application can improve the accuracy of hemoglobin detection.

The present invention provides a method for detecting hemoglobin concentration and a blood cell analyzer, wherein the method comprises: detecting a first test sample injected into a reaction cell and obtaining a first HGB value; wherein the first test sample comprises a diluent, a blood sample, and a first hemolytic agent; detecting a second test sample injected into the reaction cell and obtaining a second HGB value; wherein the second test sample is obtained by adding the second hemolytic agent to the first test sample in the reaction cell and mixing them; and selecting the minimum of the first and second HGB values as the HGB value of the blood sample. Because the presence of bubbles during the detection process can lead to an inflated HGB value, the present application proposes selecting the minimum of the first and second HGB values as the HGB value of the blood sample. This minimizes the impact of bubbles on the HGB value and improves the accuracy of the HGB detection result.

The utility model discloses a built-in hemolytic agent bottle and hematology analyzer relates to medical instrument technical field, wherein the built-in hemolytic agent bottle includes solvent bottle, movable joint block and pipeline subassembly, solvent bottle includes mutually communicated bottle body and bottle cap, bottle body and bottle cap detachable connection, bottle cap far away from bottle body side is equipped with the movable mouth; the movable connector block is movably arranged at the movable opening, the movable connector block and the movable opening are arranged in a clearance mode, and the movable connector block is provided with a mounting hole which is formed in the thickness direction in a penetrating mode; the pipeline assembly is fixedly arranged at the mounting hole, one end of the pipeline assembly extends into the bottle body, and the other end of the pipeline assembly penetrates out of the mounting hole and is communicated with an external liquid system. According to the technical scheme, the problem that when a bottle cap of an existing built-in hemolytic agent bottle is screwed down, a pipeline is prone to being bent is solved.

The utility model discloses a hemolytic agent refrigeration device. The hemolytic agent refrigeration device comprises a refrigerator main body, a placing frame and a guide plate, a refrigeration cavity is formed in the refrigerator body, an opening is formed in one side of the refrigerator body, a sealing door is installed on the refrigerator body in an opening and closing mode, and a plurality of universal wheels are installed at the bottom of the installation frame. The guide plate is installed at the lower end of the side, close to the opening, of the refrigerator body through a storage driving structure. According to the hemolytic agent cold storage device, the hemolytic agent packaging container is placed through the placing frame, the unfolded guide plate is matched, hemolytic agents placed on the placing frame can be conveniently pushed into the refrigerator body to be refrigerated, after the placing frame enters the refrigerator body, the guide plate can be automatically stored by starting the storage driving structure, and the hemolytic agent cold storage device is convenient to use. Compared with the prior art, human input is effectively reduced, and cold storage of the hemolytic agent is facilitated.

The invention discloses a hemolytic agent and a detection kit, the hemolytic agent comprises a surfactant and guanidine substances, and the pH value of the hemolytic agent is 2.8-9.5; the concentration of the guanidine substance is 0.2 g / L-12 g / L, and the guanidine substance is a guanidyl compound or guanidine salt. The surfactant can realize red blood cell hemolysis and perform different degrees of treatment on white cell membranes, besides normal contact, guanidine substances are positively charged and can attract hemoglobin with negative charges, and after the guanidine substances are combined with the hemoglobin, disulfide bonds in the hemoglobin can be promoted to be damaged, so that the hemoglobin is disintegrated. Meanwhile, the guanidine substances almost have no destructive effect on the leukocytes, so that the leukocytes still keep respective states in the hemolytic agent disclosed by the invention. In combination with a test case, compared with a traditional hemolytic agent, the hemolytic agent disclosed by the invention can be simultaneously used for detection of hemoglobin and leukocyte classification.

The invention relates to a pretreatment method of a blood culture solution for microorganism mass spectrum identification, which comprises the following steps: fully and uniformly mixing a hemolytic agent with the blood culture solution which comprises blood cells and at least one microorganism; centrifuging and filtering to obtain a microbial precipitate; wherein the hemolytic agent is used for dissolving blood cells without destroying microorganisms, the hemolytic agent comprises a nonionic surfactant, the nonionic surfactant is selected from one or more compounds shown in the formula (I), and R1 is selected from hydrogen and C1-C8 alkyl; r2 is selected from a C8-C20 fatty alcohol group, a C8-C20 fatty acid group and a C8-C20 fatty amine group; and n is 5-30. According to the method, blood cells can be rapidly dissolved, the solution viscosity and bubbling cannot be increased, the microbial separation efficiency is improved, and the microbial mass spectrum identification speed is increased.

The invention relates to a blood cell analyzer and a blood cell analysis method. Sucking a to-be-detected blood sample; mixing a part of the blood sample to be detected, a hemolytic agent and a first fluorescent staining agent to prepare a first measurement sample, and detecting first optical information of particles in the first measurement sample; mixing the other part of the blood sample to be measured, the diluent and a second fluorescent staining agent to prepare a second measurement sample, and detecting second optical information of particles in the second measurement sample; acquiring first leukocyte classification information based on the first optical information; when it is judged that classification abnormity exists in the first leukocyte classification scatter diagram, second leukocyte classification information is obtained based on the second optical information, and the second leukocyte classification information is used for correcting the first leukocyte classification information. According to the invention, an accurate leukocyte classification result can be obtained.

The disclosure provides a sample testing method and a sample analyzer, the method including: obtaining a sample to be tested; providing a reagent, the reagent including a hemolytic agent, a first fluorescent dye and a third fluorescent dye; mixing the sample and the reagent to form a sample solution; making the sample solution flow in a flow cell in a single test, irradiating the particles by using a first light source and a second light source, and detecting scattered light signals, first fluorescence signals and third fluorescence signals; obtaining a classification result and / or a counting result of white blood cells in the sample to be tested based on the first fluorescence signals and the scattered light signals; and obtaining a counting result of nucleated red blood cells in the sample to be tested based on the third fluorescence signals and the scattered light signals.

The application discloses a blood sample distribution method and a blood sample distribution device. The blood sample distribution method comprises the following steps: obtaining an initial blood sample by a sampling needle; adding a hemolytic agent into a DIFF reaction pool; distributing the initial blood sample into the DIFF reaction pool by the sampling needle to prepare a DIFF reaction liquid; adding a diluent into a WBC reaction pool and distributing the initial blood sample into the WBC reaction pool by the sampling needle to prepare a second sample liquid; cleaning the inner wall and the outer wall of the sampling needle; sucking the second sample liquid from the WBC reaction pool by the sampling needle; adding the hemolytic agent into the WBC reaction pool to prepare a WBC reaction liquid; adding the diluent into an RBC reaction pool and distributing the second sample liquid into the RBC reaction pool by the sampling needle to prepare an RBC reaction liquid. The blood sample distribution method changes the blood distribution sequence of the initial blood sample, and the blood sample is first distributed into the DIFF reaction pool which has a lower requirement for the quantitative accuracy of the blood sample, so that the blood throwing operation is not needed, the blood sample consumption is reduced, and the consumption of the cleaning reagent is saved.

The embodiments of the present application relate to a platelet counting method and a blood cell analyzer. The method comprises: aspirating a blood sample to be tested; mixing one portion of said blood sample with a diluent to prepare a first test sample, and mixing the other portion of said blood sample with a hemolytic agent and fluorescent dye to prepare a second test sample; performing impedance measurement on the first test sample, in order to obtain an electrical signal of the first test sample; performing optical measurement on the second test sample, in order to obtain at least one optical signal from among a scattered light signal and a fluorescent light signal of the second test sample; at least on the basis of the electrical signal of the first test sample, obtaining platelet distribution information of said blood sample; and at least on the basis of the pulse width of an optical signal among the at least one optical signal that corresponds to platelets in the second test sample, and the platelet distribution information, obtaining a platelet count value. Thus, an accurate platelet count can be obtained at a low cost.

The present invention has discovered that DO3A-based substances containing gadolinium complexes, which are conventionally used as anti-inflammatory agents, have a novel effect of inhibiting thrombin-induced thrombosis or dissolving blood, without being involved in the platelet action function of stopping bleeding at an injury or wound site, and discloses the applicability of DO3A-based substances containing gadolinium complexes to novel uses as thrombosis inhibitors or blood coagulants.

The present invention provides a four-classification analysis device for leukocytes, the device comprising a leukocyte cup, a positive and negative pressure pump, a diluent adding channel, and a hemolytic agent adding channel, wherein the diluent is added into the leukocyte cup through the diluent adding channel, and the hemolytic agent is added into the diluted leukocyte cup once through the hemolytic agent adding channel, and the positive and negative pressure pumps are connected to the bottom of the leukocyte cup through an air channel, and are used to blow gas into the leukocyte cup to convectively mix the diluted blood sample with the hemolytic agent. The present invention only needs to add the hemolytic agent once, so it can simplify the liquid path structure and reduce the consumption of the hemolytic agent.

According to the hematuria microscopic examination method, the urine detection reagent, the chip and the hematuria evaluation parameters, urine is diluted by M times to obtain a microscopic examination sample; or adding a hemolytic agent into the urine to obtain a microscopic examination sample; adding a microscopic examination sample into a detection cavity A of the detection chip, wherein the cavity height of the detection cavity is HA; shooting an image of the detection cavity; the cavity area corresponding to the microscopic examination image is SA; target visible components in the microscopic examination image are recognized, and the number Kn of the target visible components is obtained; the unit volume number Pn of the target visible components is equal to M * Kn / (SA * HA); the target visible component comprises red blood cells. The method is suitable for quantitative analysis of urine samples which are high in blood component content in hematuria and cannot flatten all blood cells in microscopic examination. A microscopic examination sample is obtained after the hemolytic agent is added into the urine, red blood cells can be eliminated, the red blood cells are prevented from shielding other visible components, and the detection rate of other visible components in the urine is increased.

The present invention discloses a classification cell cleaning system, method and analyzer. The cleaning system includes a waste discharge module, at least one reagent module and a cleaning module connected to the classification cell; the waste discharge module is used to empty the remaining reaction liquid in the classification cell; the reagent module is used to inject a first preset amount of hemolytic agent into the classification cell; the cleaning module is used to inject a second preset amount of diluent into the classification cell when or after injecting the hemolytic agent, so as to rinse the classification cell and empty it through the waste discharge module after rinsing; the cleaning module is used to inject a third preset amount of diluent into the classification cell after rinsing and emptying, so as to soak the classification cell and empty it through the waste discharge module after soaking; the reagent module is used to aspirate a fourth preset amount of air column after soaking and emptying. The present invention optimizes the timing control in the cleaning process, can improve the cleanliness of the classification cell, reduce the usage amount of the hemolytic agent, and reduce the cost.

The invention relates to a blood cell analyzer and a blood cell analysis method. The method comprises the following steps: mixing a part of a sucked blood sample to be detected, a diluent and a fluorescent staining agent in a first reaction tank to prepare a first determination sample, and obtaining first optical information generated after particles in the first determination sample are irradiated by light; mixing the other part of the blood sample to be detected with a hemolytic agent in a second reaction tank to prepare a second determination sample, and obtaining colorimetric optical information of the second determination sample; obtaining a reticulocyte detection result, a platelet detection result, an erythrocyte detection result and a first leukocyte detection result of the to-be-detected blood sample based on the first optical information, wherein the first leukocyte detection result at least comprises a lymphocyte percentage, a mononuclear cell percentage and a granulocyte percentage; and determining the hemoglobin concentration of the to-be-detected blood sample based on the colorimetric method optical information. According to the application, the reagent cost required for obtaining the detection result of the to-be-detected blood sample can be reduced.

The invention discloses a tumor immunotherapy curative effect prediction kit based on bone marrow spleen neutrophil proportion, which comprises an anti-human CD62L-PE antibody, an anti-human CD11b-PE-Cy7 antibody, an anti-human CD44-APC antibody and an anti-human ICAM1-FITC antibody which are respectively used for marking neutrophil subgroups in a peripheral blood sample, and comprises a hemolytic agent which is used for removing red blood cells in the sample, according to the present invention, the structure is scientific and reasonable, the use is safe and convenient, the ratio of the bone marrow-derived neutrophil to the spleen-derived neutrophil, i.e. The M / S ratio, has the significant negative correlation with the treatment effect of the PD-1 inhibitor, and the kit designed based on the discovery can achieve the rapid and non-invasive immune response evaluation at the peripheral blood level, compared with an existing method depending on tissue biopsy or PD-L1 immunohistochemistry, the method has the advantages that the immune microenvironment state of a patient can be reflected more early and dynamically, and a precise curative effect prediction basis is provided for a PD-1 / PD-L1 inhibitor and a combination therapy thereof.

The embodiment of the invention relates to a sample analysis method of a sample analyzer. The sample analysis method comprises the following steps: sucking a to-be-detected blood sample; mixing at least one part of the blood sample to be detected, the hemolytic agent, the first fluorescent dye and the second fluorescent dye to prepare a detection sample at a time; in a single test, each particle in a measurement sample is passed through an optical detection zone irradiated by excitation light to acquire optical information of the measurement sample, the excitation light including light of a first wavelength capable of exciting a first fluorescent dye and light of a second wavelength capable of exciting a second fluorescent dye, the second wavelength being greater than the first wavelength, the optical information comprises a first fluorescent signal corresponding to the first fluorescent dye and a second fluorescent signal corresponding to the second fluorescent dye, which are obtained in a single test; and obtaining infected red blood cell information of the measurement sample based on the first fluorescence signal and the second fluorescence signal. Therefore, the infected red blood cell information can be quickly and accurately obtained to assist malaria diagnosis.

Disclosed is applicability in novel uses of a DO3A-based material containing a gadolinium complex as an antithrombotic agent or a coagulant, on the basis of a discovery finding that the DO3A-based material containing the gadolinium complex which has been used as an anti-inflammatory agent has a novel effect of suppressing a thrombus induced by thrombin or dissolving blood, without being involved in the function of platelet action which stops bleeding at a damaged or injured area.

This invention discloses a method, apparatus, medium, and device for determining insufficient hemolytic agent dosage. First, a set of pulse signals from a blood cell sample under a preset scattering angle is acquired. Then, the signal intensity of each pulse signal in the set is identified, and the particle distribution is statistically analyzed. Next, the rate of change of particle count for each type of signal intensity in the particle distribution is calculated to obtain the rate of change. Then, the zero-crossing points of the rate of change are searched for, and the maximum particle count and the minimum particle count associated with red blood cells are determined based on these points. Finally, the signal interference value is calculated based on the minimum and maximum particle counts. If the signal interference value is greater than an interference threshold, the current hemolytic agent dosage is determined to be insufficient. This invention can promptly remind testing personnel to check the remaining reagent level when significant interference is detected by monitoring the degree of red blood cell interference in the pulse signal.

This invention relates to a sample processing reagent and its application in flow cytometry analysis, belonging to the field of flow cytometry analysis technology. The invention provides a sample processing reagent comprising a hemolysin and a cell-permeable agent. The hemolysin comprises tris(hydroxymethyl)aminomethane (Tris), sodium chloride, calcium chloride, disodium ethylenediaminetetraacetate, paraformaldehyde, and a solvent. The cell-permeable agent comprises sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, nonylphenol polyoxyethylene ether, Triton X-100, paraformaldehyde, and a solvent. The hemolysin is mild and highly effective, providing complete hemolysis and clear cell clustering. The cell-permeable agent permeates the cell membrane under isotonic and neutral conditions, minimizing damage to cell surface antigens and effectively detecting both cytoplasmic and nuclear antigens.

The hemolysis-based blood leukocyte recognition AI training method comprises the following steps: adding a hemolytic agent into blood to obtain a blood sample; flatly spreading the blood sample to enable white blood cells to suspend in the liquid and to present a natural state; shooting the blood sample to obtain a picture; carrying out AI training by using the obtained pictures to obtain a leukocyte AI feature data set A; in the application, the inventor puts forward that the efficiency of leukocyte classification and recognition is improved by dissolving blood cells, eliminating red blood cells, spreading leukocytes, carrying out photographing identification training AI, and staining cell nucleuses of leukocytes by adding a liquid staining agent. Through precipitation layering enrichment, the concentration of specific white blood cells is improved, and the recognition training efficiency is improved.

The present application relates to a blood cell analyzer and a blood cell analysis method. The method comprises: drawing a blood sample to be tested; mixing part of said blood sample with a hemolytic agent to prepare a first test sample, and detecting first optical information of particles in the first test sample; mixing the other part of said blood sample, a diluent and a fluorescent stain to prepare a second test sample, and detecting second optical information of particles in the second test sample; acquiring first white blood cell classification information on the basis of the first optical information; and when it is determined that there is a classification abnormality in a first white blood cell classification scatter diagram, acquiring second white blood cell classification information on the basis of the second optical information, and using the second white blood cell classification information to correct the first white blood cell classification information. The present application can obtain an accurate white blood cell classification result.

The invention relates to the technical field of medical treatment, and particularly discloses a red breaking liquid for bloody body fluid cell wax blocks. The red breaking liquid is prepared from absolute ethyl alcohol and distilled water, and the volume concentration of the absolute ethyl alcohol is 20%-30%; according to the method, the ethanol aqueous solution in a specific concentration range is adopted as a splitting system, efficient and selective splitting of the red blood cells in the bloody body fluid is realized, and cell membranes of the seedless red blood cells can be quickly destroyed by utilizing the dissolution effect of ethanol on cell membrane lipid and the hypotonic characteristic of the solution, so that contents of the seedless red blood cells are released and are easily eluted. Meanwhile, the morphological integrity and cell membrane antigenicity of nucleated cells (including tumor cells) can be protected to the greatest extent, and the problem of cell damage or antigen shielding possibly caused by a conventional hemolytic agent is avoided.

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Disclosed is applicability in novel uses of a DO3A-based material containing a gadolinium complex as an antithrombotic agent or a coagulant, on the basis of a discovery finding that the DO3A-based material containing the gadolinium complex which has been used as an anti-inflammatory agent has a novel effect of suppressing a thrombus induced by thrombin or dissolving blood, without being involved in the function of platelet action which stops bleeding at a damaged or injured area.