The invention relates to a bartonia body solid-liquid double-phase slant culture medium as well as a preparation method and an application method thereof. The slant culture medium comprises a slant solid culture medium and a liquid culture medium, wherein the slant solid culture medium comprises beef leach liquid, starch, sodium chloride, agar, defibrinated sheep blood and distilled water; and the liquid culture medium comprises trypticase hydrolysate, soybean protein and papayotin hydrolysate, glucose, sodium chloride, di-potassium phosphate and distilled water. The bartonia body solid-liquid double-phase slant culture medium prepared by the method approximately requires 48 hours to reach 107 CFU/ml, the growth speed is increased by 2.5 times, the cost is saved by 500 times (blood plate culture approximately requires 5 days, and reaching 107 CFU/ml requires 100 flat plates), and the defects of slow bacteria growth, time taking, labor taking, high cost and the like of the traditional bartonia body invitro culture method are remedied .