Tissue culture method for improving in-vitro regeneration efficiency of common head cabbage

A tissue culture and in vitro regeneration technology, applied in the field of plant biotechnology and cabbage cultivation, can solve the problems of changing the regeneration coefficient, and achieve the effects of reducing the pollution rate, increasing the induction, and increasing the induction differentiation rate.

Inactive Publication Date: 2013-06-05
JIANGSU PROVINCE JIANGSHU SEED +1
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Problems solved by technology

It aims to solve the problem of low regeneration coefficient by changing the disinfection time of different explants, the hormone ratio of adventitious buds and root induction medium

Method used

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  • Tissue culture method for improving in-vitro regeneration efficiency of common head cabbage
  • Tissue culture method for improving in-vitro regeneration efficiency of common head cabbage
  • Tissue culture method for improving in-vitro regeneration efficiency of common head cabbage

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] The embodiment of the present invention is achieved in this way, a tissue culture method for improving the regeneration efficiency of head cabbage in vitro, the tissue culture method comprises the following steps:

[0029] 1 induction

[0030] 1.1 Material collection: Take explants at the end of bolting and flowering of head cabbage, take germinated tender side branches without disease spots and insect damage, and take the 2-3 leaves on the upper part of the branches close to the heart leaves as young leaves, take stalked young leaves and Young stems on young shoots with leaves removed.

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Abstract

The invention discloses a tissue culture method for improving the in-vitro regeneration efficiency of a common head cabbage. The tissue culture method comprises the steps of at first, taking explant with high regeneration and induction rate; sterilizing by using sodium hypochlorite; carrying out dark culture on a tender leaf on an induction culture medium for 16-20 hours, and then carrying out light culture; directly carrying out the light culture on a tender stem section with an axillary bud on the induction culture medium; transferring induced bud or cluster buds on an enrichment culture medium for enrichment culture; taking a strong single bud for rooting culture; removing a sealing film at room temperature for plantlet hardening after the strong single bud is rooted; washing off the culture medium from the root; and transplanting the bud in a prepared hole plate. With the adoption of the tissue culture method provided by the invention, the problem that explant sterilization time is different can be solved, and the problem that the regeneration coefficient in an in-vitro regeneration and induction process of the common head cabbage is low can be solved. With the adoption of the tissue culture method provided by the invention, the most efficient regeneration system of the common head cabbage can be established on the aspects of the sterilization time, an explant taking part, and different hormone combinations, and lays the foundation for the storage of genetic resources of the common head cabbage and the development of breeding of common head cabbage genetic engineering.

Description

technical field [0001] The invention belongs to the technical fields of plant biotechnology and cabbage cultivation, and in particular relates to a tissue culture method for improving the regeneration efficiency of cabbage in vitro. Background technique [0002] Heading cabbage is a biennial herb of the Brassica genus in the Cruciferae family (Cruciferae), and is one of our important vegetables. Biennial herbaceous plants form leaf bulbs after the vegetative growth period in the current year. The leaf bulb enters the reproductive growth period through storage overwintering under the influence of low temperature and long sunshine in the coming year, bolting, flowering and setting seeds, and completes the life process. Heading cabbage has the characteristics of strong adaptability, disease resistance, cold resistance, high yield, good quality, easy storage and transportation, salt and alkali resistance, and easy cultivation. However, the reproduction and preservation of germ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑子松王红于利刘晓宏李建斌王神云
Owner JIANGSU PROVINCE JIANGSHU SEED
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