Fast propagation method for aquatic plant lotus flowers
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A technology for aquatic plants and lotus, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of easy pollution and browning of lotus explants, and achieve the solution of easy pollution, easy browning, heavy The effect of good performance and simple and easy method
Active Publication Date: 2012-08-22
上海孙桥现代农业联合发展有限公司 +2
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[0006] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art tha...
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Embodiment 1
[0023] According to the rapid propagation method of the aquatic plant lotus of the present embodiment, it first adopts in vitro tissue culture to obtain tissue culture seedlings, then hardens the seedlings and transfers to obtain lotus flowers, wherein the in vitro tissue culture includes the following steps:
[0024] (1) Using lotus seeds in the same year as starting materials, washing and drying the materials, and using them as explants for tissue culture and propagation. The explants were subjected to surface treatment in 75% ethanol aqueous solution for 30 seconds, and then treated in 0.1% HgCl 2 Sterilize the explants in the solution for 5-10 minutes, rinse them with sterile water for 3-5 times, put them on filter paper to absorb excess water, and complete the disinfection and sterilization of the explants. Take out the embryos on the ultra-clean workbench, inoculate them in the basic medium for cultivation, and cultivate them under the conditions of 12-14 hours of light ...
Embodiment 2
[0029] According to the rapid propagation method of the aquatic plant lotus of the present embodiment, the basic steps of its isolated tissue culture are the same as those in Example 1, the difference is that in step (2), the composition of the proliferation medium taken is MS improved medium+ZT 7mg / L+activated carbon 0.8g / L+sucrose 30g / L and carrageenan 7g / L.
Embodiment 3
[0031] According to the rapid propagation method of the aquatic plant lotus of the present embodiment, the basic steps of its in vitro tissue culture are the same as in Example 1, the difference is that in step (3), the composition of the strong seedling medium taken is MS improved medium+ ZT 5mg / L+activated carbon 0.8g / L+sucrose 30g / L and carrageenan 7g / L.
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Abstract
The invention relates to a fast propagation method for aquatic plant lotus flowers. According to the method, in vitro tissue culture is firstly adopted for obtaining tissue culture seedlings, and the in vitro tissue culture comprises the following steps that: (1) lotus flower seeds are used as initial raw materials, after the lotus flower seeds are subjected to sterilization and disinfection treatment, plumules are taken out under the aseptic condition and are inoculated into a basic culture medium to be cultured for 2 to 3 weeks, and aseptic seedlings are obtained; (2) the hypocotyl upper parts and cotyledons of the aseptic seedlings containing growth point parts are cut off and are inoculated into a propagation culture medium, the light illumination culture is carried out for 3 to 4 weeks, and tissue culture calluses are obtained; (3) buds with the length being 2 to 3cm in the tissue culture calluses obtained in the second step are cut off and are inoculated into a strong seedling culture medium to be cultured; and (4) after the seedlings in the third step grow to 4 to 6cm, the seedlings are transplanted onto a rooting culture medium for carrying out rooting culture, and the tissue culture seedlings are obtained. The fast propagation method provided by the invention solves the problems of few lotus root resources and long propagation period in the prior art, and a new path is provided for meeting the market requirements.
Description
technical field [0001] The invention relates to a rapid propagation method of aquatic plant lotus. Background technique [0002] Lotus (Nelumbo nucifera) is a perennial aquatic perennial herb of the family Nymphaeaceae, also known as lotus, and is one of the top ten traditional famous flowers in China. The lotus varieties include about 3 strains, 6 groups, 14 categories, and 38 types, including 278 varieties, and there are more than 600 varieties of ornamental lotuses. Lotus is generally distributed in subtropical and temperate regions such as Central Asia, West Asia, North America, India, China, and Japan. my country has a long history of cultivating lotus, which was cultivated as early as more than 3,000 years ago. From Heilongjiang in the north, to Hainan in the south, from Jiangsu, Zhejiang, and Taiwan in the east, to the south of Tianshan Mountain in Xinjiang in the west, except for Tibet and Inner Mongolia, there are lotus flowers distributed in swamps, wetlands, lak...
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