29 results about "Growth point" patented technology

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention relates to the technical field of tailing sand treatment, in particular to a method for repairing vanadium titano-magnetite tailing sand by utilizing plants, which comprises the following steps: ploughing and flattening the surface layer of the tailing sand, then applying an organic modifier and a pH regulator, and fully mixing with the tailing sand of 20-40cm on the surface layer; synchronously applying a microbial agent containing heteroform rhizopus in the plant growth substrate, and sowing bermuda grass; performing field management after the cynodon dactylon seedlings survive; after at least one complete growth cycle of the cynodon dactylon is completed and the cynodon dactylon enters a mature period, stubbles of 5-10 cm are reserved by using a harvesting machine for cradling so as to ensure that the cynodon dactylon keeps necessary growing points, so that the cynodon dactylon can be quickly regenerated in the next growing season, and then the cradled cynodon dactylon is treated. The phytoremediation efficiency of the vanadium titano-magnetite tailing sand can be improved, and ecological restoration, resource circulation and environmental safety are also considered.

This invention relates to the field of agricultural intelligent equipment and machine vision technology, and discloses a method for weed detection and growth point localization based on shared features. The method constructs a collaborative dataset containing weed detection labels and growth point labels, utilizes a shared feature extraction network to extract multi-scale features, and sets detection task branches and growth point localization branches in a unified feature space to achieve joint modeling of weed detection and growth point localization. Then, adaptive positive sample selection is performed based on the joint cost between candidate sample points and labeled growth points, and joint training is conducted using detection loss, growth point localization classification loss, and growth point localization regression loss. During the inference stage, the method outputs weed detection results and growth point localization results, and generates target point information. This method can reduce redundant calculations and error cascading in the multi-stage processing, and improve the accuracy of growth point localization and the stability of target point output in complex farmland scenarios.

This invention relates to the field of rubber tree cultivation technology and discloses a method for cultivating rubber tree buds based on scale buds. The method includes: planting rubber tree bud-grafted seedlings in a propagation nursery, controlling soil moisture and applying compound fertilizer to the roots to cultivate them into single-stem buds; when the top leaves are stable and mature but the leaflets at the growing point have not yet unfolded, removing the top of the second leaf foliage below the top leaves; after removing the top, using a shade net to provide shade during specific periods on sunny days; regularly spraying a bud-promoting fertilizer containing potassium dihydrogen phosphate and urea during the aging period of the top leaves; stopping the application of compound fertilizer to the roots and spraying a bud-strengthening fertilizer containing potassium dihydrogen phosphate before cutting the buds; after sawing the trunk and cutting the buds, applying a base fertilizer containing well-rotted organic fertilizer and calcium magnesium phosphate, and repeating the topping process. By removing the top at specific times, combined with shading and phased nutrient regulation, this invention promotes uniform bud sprouting, improves the lignification of the buds, and replenishes the tree's nutrient consumption in a timely manner, ensuring the continuous propagation capacity of the propagation nursery.

The invention belongs to the technical field of ecological restoration, and particularly relates to a method for restoring vegetation of a transformer substation in a high-altitude cold and arid region. The method comprises the following steps: 1) soil preparation and fertilization, and growing point planning; 2, holes are formed in the growing points, and seedlings are planted after a growth promoter is put in the holes; and 3) growing point management. According to the method, within 60-90 days, the vegetation coverage of the exposed ground surface of the transformer substation in the cold and arid region can reach the coverage degree larger than or equal to 95%, the height smaller than or equal to 15 cm, the landscape is uniform, and the overwintering survival rate is larger than or equal to 92%; reseeding is not needed in the planting period; single species, low plants and few vertical wilt objects meet the fireproof requirement of the transformer substation; the total cost is reduced by more than 40% compared with the traditional turf laying method; the whole process is less in irrigation, only 2-3 times of water replenishing are needed, water is saved by more than 80%, the defects of slow lawn building, difficulty in overwintering and high maintenance cost in the prior art are overcome, and the method has practical popularization and application value.

The invention discloses a method for inducing a garlic mutant by differentiated bulbil, which is characterized in that a phenotypic mutation plant can be obtained by stripping the differentiated bulbil and performing EMS mutagenesis on the differentiated bulbil. Compared with the prior art, the method has the advantages that the volume of the differentiated bulbil with unclosed outer leaves is reduced by 2-3 orders of magnitude, the use of a mutagenesis reagent EMS is reduced, the cost of the mutagenesis reagent is reduced, the operation is convenient, and the potential harm of the EMS reagent to human and nature is greatly reduced. According to the method, differentiated bulbils with unclosed outer leaves are adopted, internal stored leaves are not expanded, bud points are basically exposed, EMS can fully act on growing points, and the mutation rate is high. Differentiated bulbils are cultured on an indoor culture medium after EMS treatment, the survival condition is good, and survival of harmful mutations is facilitated.

The present invention discloses a method for inducing tetraploid olives, belonging to the field of olive breeding. The induction method provided by the present invention comprises treating the growth points between the cotyledons of sterile tissue-cultured olive seedlings with an induction solution, wherein the induction solution is a mixed solution containing 0.1% to 0.5% colchicine and 0.02% to 0.04% oryzalin, prepared using a 0.2% dimethyl sulfoxide (DMSO) aqueous solution as a solvent. The method of the present invention can effectively induce the production of tetraploid olives, while achieving a relatively low material mortality rate and a high material ploidy variation rate.

The present application relates to the technical field of molecular marker, in particular to an Indel molecular marker related to yellow leaves of melon seedling growth point and application thereof. The present application discovers a key candidate gene for controlling the color of leaves of melon seedling growth point MELO3C016136 , and develops an Indel molecular marker ygp2 for identifying the color change of leaves of melon seedling growth point. The molecular marker ygp2 can be used to identify the color change trait of leaves of melon seedling growth point. Only one PCR amplification and polyacrylamide gel electrophoresis are needed for the DNA of the plant to be tested. The identification result is reliable, the genotype of the target plant can be simply identified, and the genotype identification result is completely consistent with the color phenotype of the leaves of seedling growth point, which provides important technical support for creating new melon germplasm with yellow leaves of seedling growth point.

The present application relates to the technical field of agricultural machinery automation control, and particularly relates to a corn detasseling machine operation response parameter real-time adjustment control system and method, comprising: a dynamic sensing module, a plurality of multi-axis mechanical arms and end effectors thereof, and a predictive central processing and control module. The dynamic sensing module is used to obtain the real-time kinematic state of the top growth point of the target plant in a disturbed environment; the predictive central processing and control module is configured to: based on the real-time kinematic state, predict the motion range of the top growth point of the target plant in a future preset time, and based on the predicted motion range, compensatively control the action of the multi-axis mechanical arm to perform a grabbing and removing operation on the top growth point. The system changes from "passive following" to "active prediction", can effectively overcome system delay, realize accurate positioning of a high-speed swinging target, and improve work efficiency.

The present application relates to the technical field of plant breeding, and discloses a method for efficiently inducing peony subterranean bud polyploidy and application thereof, comprising subterranean bud disinfection, cell synchronization, minimally invasive pretreatment, preparation of CS- Alg / PPy photothermal response slow-release microspheres, loading of composite inducer, microsphere implantation and photothermal regulation induction, polyploidy identification screening and transplanting, wherein the composite inducer comprises colchicine, 6-BA and graphene quantum dots, can block chromosome separation, realize cell chromosome doubling, regulate growth point cell division, simultaneously repair cell damage, improve bud survival rate, assist photothermal regulation to realize precise drug release, and promote expression of polyploid plant excellent traits. The method has high induction rate, high homozygosity and high bud survival rate, is suitable for cultivation of ornamental, cut flower, medicinal and stress-resistant peonies, and is advanced and practical in technology.

The invention relates to a cultivation method of cork tree polyploidy, and relates to the technical field of plant chromosome doubling. The method comprises the following steps: spraying an inducing solution to treat a top growing point of a balsa seedling at the seedling age of 15 days so as to induce chromosome doubling; wherein the inducing liquid comprises colchicine with the concentration of 0.1%-0.4% (w / v), and a preservative; 1 / 4 MS culture medium; 6-BA with the concentration of 3 mg / L; and a surfactant Silwet L-77 with a concentration of 0.1% (v / v), the spraying treatment is specifically as follows: an induction liquid is sprayed to the apical growth point once every 24 hours, spraying is carried out for three times in total, and the spraying amount each time enables the surface of the apical growth point to be uniformly wet and liquid drops do not drop. The method not only can effectively improve the polyploidy inductivity of balsa wood, but also can control a certain death rate of balsa wood seedlings, so that more polyploidy balsa wood plants can be obtained.

To provide a new technology that enables reduction of the time required for perennial plants to reach flowering.SOLUTION: The method for promoting flowering comprises a step of grafting in which a portion including a growth point of a juvenile plant body of a perennial plant that has not formed a flower bud is used as a scion 1, and a plant that has formed a flower bud is used as a rootstock 2, wherein the juvenile plant body used as the scion 1 and the plant used as the rootstock 2 are plants belonging to the same family.SELECTED DRAWING: Figure 2

The application discloses a banana sprout growth point recognition method and system based on multi-dimensional electrochemical feature fusion. The method comprises the following steps: performing wide-frequency impedance scanning on banana bulbs; selecting a single / double CPE equivalent circuit according to Nyquist pattern morphology, and accurately extracting electrochemical features by using a frequency domain decoupling and step-by-step cross-constraint iteration strategy; after the features are logarithmized, the features are input into a heterogeneous fusion architecture composed of linear discrimination and nonlinear tree models to suppress moisture drift and mine nonlinear coupling relationships; and finally, the growth point target is determined based on a soft voting mechanism weighted fusion. The application overcomes the low-frequency physical mismatch limitation, decouples moisture and lignification camouflage interference, and realizes high-robustness and accurate recognition of the growth point.

The application relates to a cultivation method of a lightwood polyploid and relates to the technical field of plant chromosome doubling. The method comprises the following steps: top growth points of lightwood seedlings with a seedling age of 15 days are treated by spraying an inducing liquid to induce chromosome doubling; wherein the inducing liquid comprises: 0.1%-0.4% (w / v) of colchicine, 1 / 4 MS culture medium, 3 mg / L of 6-BA and 0.1% (v / v) of a surfactant Silwet L-77; the spraying treatment is specifically as follows: the top growth points are sprayed with the inducing liquid once every 24 hours, and the spraying is performed for 3 times, and the spraying amount of each time is such that the top growth points are uniformly wetted and liquid drops do not drip. The method can not only effectively improve the lightwood polyploid induction rate, but also control a certain lightwood seedling mortality rate, so that more polyploid lightwood plants can be obtained.

The application discloses a kind of laser weeding methods for targeting weed growth point positioning based on heat map, belong to the technical field of image recognition;Including the following steps: step one: the image containing weed is collected, and training data set is obtained after processing;Step two: the target modeling of weed growth point accurate positioning is carried out through training data set, and weed target detection model is obtained, and weed target detection model adopts multi-scale feature fusion method to identify the boundary box of weed in image;Step three: according to the boundary box of weed identified by weed target detection model, heat map prediction model is established and trained;Heat map is generated by prediction model;Step four: the coordinates of the predicted growth point are converted into the physical coordinates of the laser beam by converting heat map into the predicted growth point coordinates, and the predicted growth point coordinates are converted into the physical coordinates of the laser beam.The application adopts the above method, avoids traditional broad net mode, and can quickly and accurately accurately position the core growth point of weed through the mode of heat map, which facilitates subsequent laser attack on weeds.

This invention relates to a tissue culture method for the germination of sterile Suaeda salsa seedlings. The method includes the following steps: (1) cleaning the harvested Suaeda salsa; (2) sterilizing the Suaeda salsa with 75% alcohol and 10% sodium hypochlorite in sequence; (3) selecting Suaeda salsa stem segments containing growth points and inoculating them in a culture medium; the culture medium consists of a basal culture medium and a growth inducer, and the growth inducer consists of a first growth inducer and a second growth inducer. The stem segments used in this invention for tissue culture of sterile Suaeda salsa seedlings are widely available and can be obtained directly from existing plants without relying on seed resources. The experimental steps of seed germination can be skipped, shortening the germination time to less than 15 days, increasing the germination rate, shortening the time to obtain propagated seedlings, and reducing propagation costs.

The invention discloses a method for obtaining a transgenic or gene-edited plant, which comprises the following steps of: firstly, culturing a plant seedling, and obtaining a plant to be infected when the stem length is greater than 1cm; the method comprises the following steps: transforming a target vector into agrobacterium, selecting a monoclonal bacterial colony with the target vector, preparing an agrobacterium solution, cutting a wound on a stem of a plant to be infected, externally applying the activated agrobacterium solution to the wound for infection, then carrying out illumination culture, removing a growing point after a period of time, and continuing to culture. And obtaining a transgenic plant or a gene editing plant when callus tissue grows on the wound and a new bud containing the target gene is obtained. According to the method, a wound is generated on a stem of a plant, growing point removal operation is carried out after true leaves grow out, and wound calluses are issued under the stimulation of hormone of the plant to breed into buds. The method is not limited to tissue culture and sterile operation, does not need root regeneration buds, does not need external application of hormone and does not need root regeneration induction, the operation steps are simple, and transgenic or gene edited plants can be obtained in a short time.

The invention discloses a banana bud growing point identification method and system based on multi-dimensional electrochemical feature fusion. The method comprises the following steps: carrying out broadband impedance scanning on banana corms; selecting a single / double CPE equivalent circuit according to the Nyquist graph form, and accurately extracting electrochemical characteristics by adopting a frequency domain decoupling and step-by-step cross constraint iteration strategy; after logarithmizing the features, inputting the features into a heterogeneous fusion architecture formed by linear discrimination and a nonlinear tree model so as to inhibit moisture drift and excavate a nonlinear coupling relationship; and finally, based on a soft voting mechanism, weighting and fusing to judge a growth point target. According to the method, the limitation of low-frequency physical mismatch is overcome, moisture and lignification camouflage interference is decoupled, and high-robustness accurate identification of the growing point is realized.

The invention relates to the technical field of bioengineering, in particular to a non-tissue culture hairy root induction method suitable for Chinese cabbages with different ploidy and application. The method provided by the invention comprises the following steps: infecting rootless seedlings by using agrobacterium rhizogenes transformed by a target vector, and continuously culturing to obtain transgenic hairy roots; a K599 strain is selected as the agrobacterium rhizogenes; the infection time is 8 to 20 minutes; beveled Chinese cabbage seedlings are selected as rootless seedlings, and the beveled position is located at the position 1-1.5 cm below a growing point. The method has the advantages that a plant tissue culture process is omitted, Chinese cabbage seedlings dibbled in a matrix are directly selected, the operation cost and the operation difficulty are greatly reduced, and the operation process is simplified; the method has high induction efficiency on hairy root induction of diploid and tetraploid Chinese cabbages, is wide in adaptability, and provides important technical support for research of tetraploid Chinese cabbages. And a theoretical basis is provided for Chinese cabbage gene function analysis and biosynthesis of specific metabolites.

The invention discloses a breeding method for delaying procambarus clarkii to appear on the market in summer in the technical field of aquatic animal breeding, and relates to six links of breeding pond transformation, oxygenation setting, aquatic plant transplanting and management, offspring seed putting, water quality management and fermented compound feed feeding. The fermented compound feed is prepared by the following steps: adding a mixed strain consisting of saccharomycetes and aspergillus oryzae into water to prepare a mixed bacterial liquid, mixing schizophyllumcommuneh polysaccharide, synanthrin, the special feed for procambarus clarkii and the mixed bacterial liquid, and fermenting. The average weight of the procambarus clarkii obtained through the method can reach 35 g or above, the survival rate reaches 85% or above, normal marketing of the procambarus clarkii in the summer high-temperature season is achieved, the market requirement is met, and the method is a new growth point for improving the quality and efficiency of the procambarus clarkii breeding industry.

The present application provides a soybean growth point detection emergence rate method fused with a super-resolution algorithm, which solves the problems of detail loss of growth points caused by low resolution of unmanned aerial vehicle high-altitude aerial image, lack of optimization of existing super-resolution technology for agricultural scenes, and low detection accuracy caused by overlapping of densely planted seedlings. The present application is realized by the following steps: obtaining soybean seedling stage image and pretreating; inputting the standardized image dataset into a super-resolution model based on RealESRGAN architecture and embedded with a unified adaptive multi-branch high-frequency enhancement module for super-resolution reconstruction to obtain a high-resolution reconstructed image; inputting the high-resolution reconstructed image into a YOLOv8 growth point detection model to output growth point detection results; eliminating false detection boxes with confidence lower than a set threshold, counting the number of effective growth points and calculating the emergence rate. The present application balances high-altitude collection efficiency and detection accuracy, has low relative error, and is suitable for automatic detection of soybean seedling emergence rate.

The invention discloses a laser weeding method for targeted weed growing point positioning based on a heat map, and belongs to the technical field of image recognition. Comprising the following steps: 1, collecting an image containing weeds, and processing the image to obtain a training data set; step 2, target modeling of weed growth point accurate positioning is carried out through the training data set, a weed target detection model is obtained, and the weed target detection model adopts a multi-scale feature fusion method to identify a bounding box of weeds in the image; step 3, establishing and training a heat map prediction model according to a bounding box of weeds identified by the weed target detection model; generating a heat map through the prediction model; and 4, converting the heat map into a coordinate of a predicted growing point, and converting the coordinate of the predicted growing point into a physical coordinate of a laser beam. By the adoption of the method, a traditional wide net casting mode is avoided, the weed core growing point can be rapidly and accurately positioned in a heat map mode, and follow-up laser striking on weeds is facilitated.

The invention provides application of silicon dioxide nanoparticles in promoting moss leaf incision stem cell development and a method, and relates to the technical field of plant regeneration. Depending on the totipotency of the plant stem cells, the silicon dioxide nanoparticles are used for promoting the development of the stem cells of the in-vitro moss leaves subjected to mechanical damage, promoting the rapid growth of leaf incisions, generating more new growth points, remarkably increasing the number of the epiphytic points of the newly born protonema of the leaves, shortening the cultivation time and improving the cultivation efficiency; and an effective nanometer strategy is provided for rapid propagation of moss.

The present application relates to the field of blueberry planting, in particular to a planting method for promoting early maturity of blueberry under open ground substrate cultivation conditions, the planting method comprises the following steps: after fruit harvesting, regulating the EC value of liquid inlet and liquid outlet; updating the crown and reshaping the growth framework; controlling the height and growth point of new shoots; interval control of fertilizer and water; foliar fertilization. The planting method can effectively coordinate the balance relationship between vegetative growth and reproductive growth, promote flower bud differentiation, make the maturity period of blueberry advance to the end of January to the beginning of February, which is beneficial to improve the quality, advance the concentrated market, enhance the market competitiveness, and improve the income.

The invention discloses a method for rapidly identifying chromosome ploidy of a cucumber horticultural variety. The method mainly comprises the following steps: preparing cucumber somatic cell interphase nuclear flakes; preparing a 5S rDNA (ribosomal deoxyribonucleic acid) probe / centromere sequence (Type-III) probe; and carrying out FISH hybridization. FISH hybridization signals of the 5S rDNA probe / centromere sequence (Type-III) probe are very stable in the whole cell cycle of the cucumber and are convenient to count, so that the chromosome ploidy of the cucumber variety can be accurately identified no matter whether the cell is in an interphase or a division phase. The invention provides a rapid and accurate identification method for chromosome ploidy identification of cucumber haploids or some growing point materials difficult to obtain root tips and the like.

The application discloses a kind of grafting scion tree seedling processing device, including scion tree first cutting transport module, seedling posture first alignment module, seedling posture second alignment module, scion tree after processing transport module, scion tree secondary end cutting module, scion tree growth point alignment adjustment module, cotyledon direction adjustment module;Seedling cup is transported to cotyledon direction adjustment module by distance-changing and direction-changing swing arm module such as conveyer, seedling cup and completes cotyledon position adjustment;Seedling posture first alignment module and seedling posture second alignment module carry out seedling plant righting operation;Scion tree first cutting transport module cuts scion tree and is transported to scion tree growth point alignment adjustment module and detects growth point, after longitudinal attitude adjustment, scion tree secondary end cutting module carries out the secondary cutting of scion tree;It is sent to grafting module based on grafting clamp equidistant sorting mechanism by scion tree after processing transport module, enters next process.This scheme is more accurate to carry out the cutting processing of scion tree.

The invention discloses a culture method of blueberry virus-free seedlings. The culture method comprises the following steps: acquiring disinfected blueberry branches containing buds; the method comprises the following steps: pre-treating buds of which the outer-layer leaves of the buds are stripped and growing points are exposed under the conditions that the temperature is 3.5-4.5 DEG C, the humidity is 60%-70%, and dark treatment is carried out for 60-84 hours; performing gradient heat treatment on the buds under the conditions that the temperature is 29-31 DEG C, the illumination intensity is 1800-2200 lux and the illumination time is 10-14 h / d in the first day to the third day; from the fourth day to the seventh day, the temperature ranges from 34 DEG C to 36 DEG C, the illumination intensity ranges from 2200 lux to 2700 lux, and the illumination time ranges from 10 h / d to 14 h / d; from the 8th day to the 14th day, the temperature ranges from 37 DEG C to 39 DEG C, the illumination intensity ranges from 3300 lux to 3800 lux, and the illumination time ranges from 10 h / d to 14 h / d; performing adventitious bud culture on the blueberry stem tip containing the leaf primordium; carrying out multiplication culture; performing rooting culture to obtain blueberry plants.

The invention discloses an agrobacterium rhizogenes-mediated celery hairy root genetic transformation method, and belongs to the technical field of plant transgenosis. The technical method comprises the following steps: culturing celery seedlings; preparing infected flat plate bacteria containing visual genes; the inoculating needle punctures, smears, infects and induces the transgenic hairy roots, infects the coated bacterial liquid, when the selected seedlings are robust and have about 1-2 cotyledons during infection, the infected part is 0.5-2 cm below the growing point, and the red transgenic hairy roots can be induced at the punctured part through co-culture and rooting culture in a high-humidity environment. The technical method can be used for conveniently and rapidly obtaining the visual celery transgenic hairy roots, and is of great significance to further development of celery functional gene verification and root system basic research work.

The invention discloses a mung bean genetic transformation method. The method comprises the following steps: disinfecting mung beans; placing the disinfected mung beans on a culture medium to obtain imbibition seeds; removing seed coats of the mung beans, and dissecting to expose growing points of embryo tips to obtain explants; radicles of the explants are cut off; and carrying out infection treatment on the explant without radicles by adopting an agrobacterium infection solution. The mung bean genetic transformation method does not depend on tissue culture, does not need to consider a regeneration problem existing in tissue culture and a transformation barrier caused by genotype difference, effectively improves the transformation efficiency of mung beans, and has the characteristics of simplicity and convenience in operation, low requirements on environment and operation, and easiness in large-scale production.