45 results about "Growth point" patented technology

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

The invention discloses a tissue culture method of a double-petal hemerocallis fulva scapes, which comprises the following steps: taking a double-petal hemerocallis fulva scapes, taking a growing point as a center, transversely cutting into long sections which are not less than 5cm, washing and disinfecting; putting the disinfected long segments into enzymatic hydrolysate for cell wall enzymolysis, taking out the long segments after enzymolysis is completed, and washing the long segments with sterile water; transversely cutting the long section subjected to enzymolysis into small sections of 1-2cm by taking the growing point as the center; and carrying out tissue culture by taking the small sections as explants. The method applies the double-petal hemerocallis fulva variety, and has the advantages that materials can be taken at any time in the flowering phase, the tissue culture efficiency is high, and the quality is good.

The invention belongs to the technical field of sampling detection and analysis of plant diseases and insect pests, and particularly discloses an intelligent sampling detection and analysis method for crop plant diseases and insect pests, which comprises the following steps: searching growth points of abnormal plants through spectrum equipment and positioning equipment carried by an unmanned aerial vehicle, and dividing corresponding sampling areas of different abnormal types; determining the sampling quantity in the areas by analyzing the air environment and the soil environment of different sampling areas, determining the sampling positions in the areas by dividing the unusual degree grades of unusual plant growing points, generating a sampling environment information set, and importing data as a training model; meanwhile, a biochemical feature set of the sampled leaves is generated through biochemical measurement and serves as exported data, a training model is established, and the mapping relation from leaf features and environmental data to biochemical features is reflected. According to the mode, the dynamic adjustment of a sampling strategy is realized, and the scientificity and representativeness of crop detection analysis are improved.

The invention relates to the technical field of tailing sand treatment, in particular to a method for repairing vanadium titano-magnetite tailing sand by utilizing plants, which comprises the following steps: ploughing and flattening the surface layer of the tailing sand, then applying an organic modifier and a pH regulator, and fully mixing with the tailing sand of 20-40cm on the surface layer; synchronously applying a microbial agent containing heteroform rhizopus in the plant growth substrate, and sowing bermuda grass; performing field management after the cynodon dactylon seedlings survive; after at least one complete growth cycle of the cynodon dactylon is completed and the cynodon dactylon enters a mature period, stubbles of 5-10 cm are reserved by using a harvesting machine for cradling so as to ensure that the cynodon dactylon keeps necessary growing points, so that the cynodon dactylon can be quickly regenerated in the next growing season, and then the cradled cynodon dactylon is treated. The phytoremediation efficiency of the vanadium titano-magnetite tailing sand can be improved, and ecological restoration, resource circulation and environmental safety are also considered.

The invention relates to the technical field of smart agriculture, in particular to a smart agricultural plant full view measurement and calibration method and system based on computer vision, and the method comprises the steps: building a region simulation model for a planting region, and measuring the longitudinal distance between any adjacent target plants in the region simulation model according to the development starting time of the target plants, the region division module sets an independent growth region based on a longitudinal distance value; the image analysis module further identifies a relationship between branches and leaves and growth points in a unit image, distinguishes associated branches and leaves from irrelevant branches and leaves, accurately judges an interference state, and then marks and measures the associated branches and leaves according to the interference state; accurate plant growth information is obtained, so that the measurement result can more truly reflect the actual growth condition of a single plant, and further accurate measurement of the full view of the plant is realized.

This invention relates to the field of agricultural intelligent equipment and machine vision technology, and discloses a method for weed detection and growth point localization based on shared features. The method constructs a collaborative dataset containing weed detection labels and growth point labels, utilizes a shared feature extraction network to extract multi-scale features, and sets detection task branches and growth point localization branches in a unified feature space to achieve joint modeling of weed detection and growth point localization. Then, adaptive positive sample selection is performed based on the joint cost between candidate sample points and labeled growth points, and joint training is conducted using detection loss, growth point localization classification loss, and growth point localization regression loss. During the inference stage, the method outputs weed detection results and growth point localization results, and generates target point information. This method can reduce redundant calculations and error cascading in the multi-stage processing, and improve the accuracy of growth point localization and the stability of target point output in complex farmland scenarios.

This invention relates to the field of rubber tree cultivation technology and discloses a method for cultivating rubber tree buds based on scale buds. The method includes: planting rubber tree bud-grafted seedlings in a propagation nursery, controlling soil moisture and applying compound fertilizer to the roots to cultivate them into single-stem buds; when the top leaves are stable and mature but the leaflets at the growing point have not yet unfolded, removing the top of the second leaf foliage below the top leaves; after removing the top, using a shade net to provide shade during specific periods on sunny days; regularly spraying a bud-promoting fertilizer containing potassium dihydrogen phosphate and urea during the aging period of the top leaves; stopping the application of compound fertilizer to the roots and spraying a bud-strengthening fertilizer containing potassium dihydrogen phosphate before cutting the buds; after sawing the trunk and cutting the buds, applying a base fertilizer containing well-rotted organic fertilizer and calcium magnesium phosphate, and repeating the topping process. By removing the top at specific times, combined with shading and phased nutrient regulation, this invention promotes uniform bud sprouting, improves the lignification of the buds, and replenishes the tree's nutrient consumption in a timely manner, ensuring the continuous propagation capacity of the propagation nursery.

The present invention belongs to the technical field of plant regeneration, and specifically relates to a method for promoting the increase of the growth points of Leucobryum glaucum leaves, a method for rapid regeneration, and applications. The present invention provides a method for promoting the increase of the growth points of Leucobryum glaucum leaves and a method for inducing the rapid regeneration of Leucobryum glaucum leaves. The excised leaves of Leucobryum glaucum are treated with brassinolide to promote the generation of new growth points on the excised leaves of Leucobryum glaucum, realizing the rapid growth of Leucobryum glaucum leaves, greatly shortening the cultivation time, and making up for the inherent defect of the slow growth of bryophytes.

The invention belongs to the technical field of ecological restoration, and particularly relates to a method for restoring vegetation of a transformer substation in a high-altitude cold and arid region. The method comprises the following steps: 1) soil preparation and fertilization, and growing point planning; 2, holes are formed in the growing points, and seedlings are planted after a growth promoter is put in the holes; and 3) growing point management. According to the method, within 60-90 days, the vegetation coverage of the exposed ground surface of the transformer substation in the cold and arid region can reach the coverage degree larger than or equal to 95%, the height smaller than or equal to 15 cm, the landscape is uniform, and the overwintering survival rate is larger than or equal to 92%; reseeding is not needed in the planting period; single species, low plants and few vertical wilt objects meet the fireproof requirement of the transformer substation; the total cost is reduced by more than 40% compared with the traditional turf laying method; the whole process is less in irrigation, only 2-3 times of water replenishing are needed, water is saved by more than 80%, the defects of slow lawn building, difficulty in overwintering and high maintenance cost in the prior art are overcome, and the method has practical popularization and application value.

The invention relates to the technical field of cucumber grafting, and particularly discloses a cucumber single-shoulder oblique splitting grafting method which comprises the following steps: step 1, a preparation stage: S1, selecting pumpkin seedlings which grow robustly, have developed root systems and are high in disease resistance as rootstocks; s2, cucumber seedlings which grow well and are free of plant diseases and insect pests are selected as scions; according to the method, one cotyledon is cut off through an oblique cleaving method with growing points in stock treatment, the grafting speed is greatly increased, the later problem that the growing points are not thoroughly removed is avoided, tight and stable combination of scions and stocks is achieved by accurately controlling the oblique cleaving angle and the attaching connection mode, and the grafting efficiency is improved. Water loss and germ invasion in the grafting process are effectively reduced, so that the grafting survival rate is remarkably increased; compared with a traditional grafting method, the method has the advantages that the operation steps are simplified, the strict requirements for the sizes, shapes and the like of scions and stocks are reduced, the difficulty of the grafting technology is lowered, and more farmers and gardening workers can easily master and apply the method.

The invention relates to the technical field of microorganisms, in particular to a fermentation medium for improving enzyme activity of lignin degrading enzyme produced by white-rot fungi and application thereof.The fermentation medium comprises a culture container and a controller, the culture container is provided with a driving part, and the driving part is used for driving the culture container to rotate; the controller is used for dividing a fermentation period based on the fermentation time, and the fermentation period comprises a hypha expansion period, an environment coordination period and a fermentation stable period; in the hypha expansion period, the controller controls the driving piece to enable the rotating speed of the culture container to be 400-800 r / min, so that the hypha is cut off by shearing force generated by rotation, and a new growing point is formed; in the environment coordination period, the controller controls the driving piece to enable the rotating speed of the culture container to be 300-400 r / min; and the fermentation stable phase controller controls the driving piece to enable the rotating speed of the culture container to be 100-200r / min. With the adoption of the technical scheme, the rotating speed of the fermentation culture medium is dynamically controlled, so that the effects of dispersing hyphae, cutting the hyphae and damaging the mycelia generated by shearing force of rotation can be regulated in a targeted manner in each hypha growth stage, and the improvement of enzyme activity is promoted.

The present invention relates to a local temperature control module for crop cultivation and, more specifically, to a local temperature control module for crop cultivation, comprising: a local temperature control unit in which a fluid movement passage is formed, and which is arranged to be adjacent to a growth point of a target crop so as to control the temperature in the vicinity of the growth point of the target crop; a fluid supply unit for supplying a temperature control fluid to the fluid movement passage of the local temperature control unit; and a fluid discharge unit for discharging the temperature control fluid in the fluid movement passage.

The invention discloses a method for inducing a garlic mutant by differentiated bulbil, which is characterized in that a phenotypic mutation plant can be obtained by stripping the differentiated bulbil and performing EMS mutagenesis on the differentiated bulbil. Compared with the prior art, the method has the advantages that the volume of the differentiated bulbil with unclosed outer leaves is reduced by 2-3 orders of magnitude, the use of a mutagenesis reagent EMS is reduced, the cost of the mutagenesis reagent is reduced, the operation is convenient, and the potential harm of the EMS reagent to human and nature is greatly reduced. According to the method, differentiated bulbils with unclosed outer leaves are adopted, internal stored leaves are not expanded, bud points are basically exposed, EMS can fully act on growing points, and the mutation rate is high. Differentiated bulbils are cultured on an indoor culture medium after EMS treatment, the survival condition is good, and survival of harmful mutations is facilitated.

The present invention discloses a method for inducing tetraploid olives, belonging to the field of olive breeding. The induction method provided by the present invention comprises treating the growth points between the cotyledons of sterile tissue-cultured olive seedlings with an induction solution, wherein the induction solution is a mixed solution containing 0.1% to 0.5% colchicine and 0.02% to 0.04% oryzalin, prepared using a 0.2% dimethyl sulfoxide (DMSO) aqueous solution as a solvent. The method of the present invention can effectively induce the production of tetraploid olives, while achieving a relatively low material mortality rate and a high material ploidy variation rate.

The invention relates to the technical field of plant polyploidy breeding, and provides a method for inducing dandelion chromosome doubling by colchicine, which comprises the following steps: sowing and germinating to obtain seedlings, and coating plant growing points of the seedlings with a colchicine solution for induction treatment to obtain dandelion with doubled chromosomes. According to the technical scheme, the problems that traditional breeding of the alkaline land dandelions is difficult, novel breeding technologies are deficient, good varieties are few, biomass of some high-quality varieties is small, and industrial development of the alkaline land dandelions is affected in the prior art are solved.

The present application relates to the technical field of molecular marker, in particular to an Indel molecular marker related to yellow leaves of melon seedling growth point and application thereof. The present application discovers a key candidate gene for controlling the color of leaves of melon seedling growth point MELO3C016136 , and develops an Indel molecular marker ygp2 for identifying the color change of leaves of melon seedling growth point. The molecular marker ygp2 can be used to identify the color change trait of leaves of melon seedling growth point. Only one PCR amplification and polyacrylamide gel electrophoresis are needed for the DNA of the plant to be tested. The identification result is reliable, the genotype of the target plant can be simply identified, and the genotype identification result is completely consistent with the color phenotype of the leaves of seedling growth point, which provides important technical support for creating new melon germplasm with yellow leaves of seedling growth point.

The present application relates to the technical field of agricultural machinery automation control, and particularly relates to a corn detasseling machine operation response parameter real-time adjustment control system and method, comprising: a dynamic sensing module, a plurality of multi-axis mechanical arms and end effectors thereof, and a predictive central processing and control module. The dynamic sensing module is used to obtain the real-time kinematic state of the top growth point of the target plant in a disturbed environment; the predictive central processing and control module is configured to: based on the real-time kinematic state, predict the motion range of the top growth point of the target plant in a future preset time, and based on the predicted motion range, compensatively control the action of the multi-axis mechanical arm to perform a grabbing and removing operation on the top growth point. The system changes from "passive following" to "active prediction", can effectively overcome system delay, realize accurate positioning of a high-speed swinging target, and improve work efficiency.

A method for obtaining regenerated seedlings of Brassica campestris L. ssp. chinensis from embryonic tip tissues, including the following steps. A seed is inoculated to a germination medium for dark culture for 60 h. The testa, root tip, two cotyledons and middle growing point of the resultant germinated seed are removed, and an embryonic tip with a length of 3-5 mm is retained as an explant. The explant is sequentially subjected to low-temperature pre-culture in a pre-culture medium for 36 h, room-temperature shaking culture in a liquid bud induction culture medium for 10 min, and bud induction culture in a bud induction culture medium for 20 d. A regenerated plant with 5-6 leaves is transferred to a rooting medium for rooting culture for about 2 weeks, and a well-rooted plant is collected, and subjected to hardening and transplantation into a filed.

The invention discloses a grafted scion seedling treatment device which comprises a scion primary cutting and transporting module, a seedling posture primary calibration module, a seedling posture secondary calibration module, a treated scion transporting module, a scion secondary tail end cutting module, a scion growing point calibration and adjustment module and a cotyledon direction adjustment module. The seedling cups are conveyed to the cotyledon direction adjusting module through the conveying belt and the equal-distance-changing and direction-changing swing arm module, so that cotyledon position adjustment is completed; the seedling posture first-stage calibration module and the seedling posture second-stage calibration module are used for strengthening seedlings; the scion primary cutting and transporting module is used for cutting scions and transporting the scions to the scion growing point calibration and adjustment module for detecting growing points, and the scion secondary tail end cutting module is used for carrying out secondary cutting on the scions after the longitudinal posture is adjusted; the scion wood is conveyed to the grafting module based on the grafting clamp equidistant sequencing mechanism by the scion wood processing and conveying module, and the next process is carried out. According to the scheme, cutting treatment of the scion wood can be more accurately carried out.

The invention provides an application of a GhFL gene in regulating and controlling the flowering phase and plant type of cotton, and belongs to the technical field of gene engineering. According to the GhFL gene efficiently expressed in the cotton stem tip, the GhFL protein is positioned in a cell nucleus and plays an important role in plant flowering nuclear plant type regulation and control. After the gene is silenced in cotton, the growth of the top end of a main stem of a plant is inhibited, a growing point is replaced by a flower bud, flowering is advanced, and the expression quantity of GhAP1 and GhCAL in a GhFL gene silenced plant is remarkably increased, which indicates that the GhFL gene can influence the flowering time and plant type by influencing the expression of a flowering related gene. Therefore, the GhFL gene and the encoding protein thereof can regulate and control the flowering time and the plant type of the plant, and a new molecular target is provided for early maturing and plant type improvement of cotton.

The present application relates to the technical field of plant breeding, and discloses a method for efficiently inducing peony subterranean bud polyploidy and application thereof, comprising subterranean bud disinfection, cell synchronization, minimally invasive pretreatment, preparation of CS- Alg / PPy photothermal response slow-release microspheres, loading of composite inducer, microsphere implantation and photothermal regulation induction, polyploidy identification screening and transplanting, wherein the composite inducer comprises colchicine, 6-BA and graphene quantum dots, can block chromosome separation, realize cell chromosome doubling, regulate growth point cell division, simultaneously repair cell damage, improve bud survival rate, assist photothermal regulation to realize precise drug release, and promote expression of polyploid plant excellent traits. The method has high induction rate, high homozygosity and high bud survival rate, is suitable for cultivation of ornamental, cut flower, medicinal and stress-resistant peonies, and is advanced and practical in technology.

The invention relates to a cultivation method of cork tree polyploidy, and relates to the technical field of plant chromosome doubling. The method comprises the following steps: spraying an inducing solution to treat a top growing point of a balsa seedling at the seedling age of 15 days so as to induce chromosome doubling; wherein the inducing liquid comprises colchicine with the concentration of 0.1%-0.4% (w / v), and a preservative; 1 / 4 MS culture medium; 6-BA with the concentration of 3 mg / L; and a surfactant Silwet L-77 with a concentration of 0.1% (v / v), the spraying treatment is specifically as follows: an induction liquid is sprayed to the apical growth point once every 24 hours, spraying is carried out for three times in total, and the spraying amount each time enables the surface of the apical growth point to be uniformly wet and liquid drops do not drop. The method not only can effectively improve the polyploidy inductivity of balsa wood, but also can control a certain death rate of balsa wood seedlings, so that more polyploidy balsa wood plants can be obtained.

To provide a new technology that enables reduction of the time required for perennial plants to reach flowering.SOLUTION: The method for promoting flowering comprises a step of grafting in which a portion including a growth point of a juvenile plant body of a perennial plant that has not formed a flower bud is used as a scion 1, and a plant that has formed a flower bud is used as a rootstock 2, wherein the juvenile plant body used as the scion 1 and the plant used as the rootstock 2 are plants belonging to the same family.SELECTED DRAWING: Figure 2

The invention discloses a field weed growing point detection model and method based on key points. Backbone is used for receiving a field weed image to realize learning of multi-scale weed image features and outputting feature maps of different stages; the Neck uses multi-scale fusion to fuse different stages of feature maps output by the Backbone, and the performance ability of weed features is enhanced; and the Head converts the feature map output by the Neck into specific information required by weed detection, wherein the specific information comprises category, position and confidence information of weeds. The constructed detection model can maintain high detection precision in a natural field environment, the model is light, the real-time detection capability is improved, and the growth points of weeds can be accurately identified in real time in the field environment. By adopting the key point detection method, the specific position coordinates of the growth points of the weeds can be directly output, and the problem that the traditional weed detection method can only detect the whole or part of the weeds and is difficult to determine the growth points is solved.

The application discloses a banana sprout growth point recognition method and system based on multi-dimensional electrochemical feature fusion. The method comprises the following steps: performing wide-frequency impedance scanning on banana bulbs; selecting a single / double CPE equivalent circuit according to Nyquist pattern morphology, and accurately extracting electrochemical features by using a frequency domain decoupling and step-by-step cross-constraint iteration strategy; after the features are logarithmized, the features are input into a heterogeneous fusion architecture composed of linear discrimination and nonlinear tree models to suppress moisture drift and mine nonlinear coupling relationships; and finally, the growth point target is determined based on a soft voting mechanism weighted fusion. The application overcomes the low-frequency physical mismatch limitation, decouples moisture and lignification camouflage interference, and realizes high-robustness and accurate recognition of the growth point.

The application relates to a cultivation method of a lightwood polyploid and relates to the technical field of plant chromosome doubling. The method comprises the following steps: top growth points of lightwood seedlings with a seedling age of 15 days are treated by spraying an inducing liquid to induce chromosome doubling; wherein the inducing liquid comprises: 0.1%-0.4% (w / v) of colchicine, 1 / 4 MS culture medium, 3 mg / L of 6-BA and 0.1% (v / v) of a surfactant Silwet L-77; the spraying treatment is specifically as follows: the top growth points are sprayed with the inducing liquid once every 24 hours, and the spraying is performed for 3 times, and the spraying amount of each time is such that the top growth points are uniformly wetted and liquid drops do not drip. The method can not only effectively improve the lightwood polyploid induction rate, but also control a certain lightwood seedling mortality rate, so that more polyploid lightwood plants can be obtained.

The application discloses a kind of laser weeding methods for targeting weed growth point positioning based on heat map, belong to the technical field of image recognition;Including the following steps: step one: the image containing weed is collected, and training data set is obtained after processing;Step two: the target modeling of weed growth point accurate positioning is carried out through training data set, and weed target detection model is obtained, and weed target detection model adopts multi-scale feature fusion method to identify the boundary box of weed in image;Step three: according to the boundary box of weed identified by weed target detection model, heat map prediction model is established and trained;Heat map is generated by prediction model;Step four: the coordinates of the predicted growth point are converted into the physical coordinates of the laser beam by converting heat map into the predicted growth point coordinates, and the predicted growth point coordinates are converted into the physical coordinates of the laser beam.The application adopts the above method, avoids traditional broad net mode, and can quickly and accurately accurately position the core growth point of weed through the mode of heat map, which facilitates subsequent laser attack on weeds.

This invention relates to a tissue culture method for the germination of sterile Suaeda salsa seedlings. The method includes the following steps: (1) cleaning the harvested Suaeda salsa; (2) sterilizing the Suaeda salsa with 75% alcohol and 10% sodium hypochlorite in sequence; (3) selecting Suaeda salsa stem segments containing growth points and inoculating them in a culture medium; the culture medium consists of a basal culture medium and a growth inducer, and the growth inducer consists of a first growth inducer and a second growth inducer. The stem segments used in this invention for tissue culture of sterile Suaeda salsa seedlings are widely available and can be obtained directly from existing plants without relying on seed resources. The experimental steps of seed germination can be skipped, shortening the germination time to less than 15 days, increasing the germination rate, shortening the time to obtain propagated seedlings, and reducing propagation costs.

The present invention discloses a method for rapidly propagating variant materials with excellent peanut traits, and belongs to the technical field of crop propagation. The method for rapidly propagating variant materials with excellent peanut traits comprises the following steps: removing the growth point of the variant material with excellent peanut traits to make a scion, and using the original receptor material of the variant material as a rootstock for grafting, so as to achieve rapid propagation of the variant material with excellent peanut traits. The present invention utilizes grafting technology to asexually propagate variant plants that exhibit excellent variant traits after mutagenesis, and F1 plants that exhibit hybrid vigor after hybridization. Removing the growth point after the peanut plant has pods will not have an adverse effect on the development of the pods, and the normal fruiting of the original plant can be retained to the greatest extent; after the grafted seedlings have pods, they can be grafted again, thereby increasing the asexual propagation coefficient without limit, providing an effective way to retain and purify the excellent peanut variant materials.

The invention discloses a method for obtaining a transgenic or gene-edited plant, which comprises the following steps of: firstly, culturing a plant seedling, and obtaining a plant to be infected when the stem length is greater than 1cm; the method comprises the following steps: transforming a target vector into agrobacterium, selecting a monoclonal bacterial colony with the target vector, preparing an agrobacterium solution, cutting a wound on a stem of a plant to be infected, externally applying the activated agrobacterium solution to the wound for infection, then carrying out illumination culture, removing a growing point after a period of time, and continuing to culture. And obtaining a transgenic plant or a gene editing plant when callus tissue grows on the wound and a new bud containing the target gene is obtained. According to the method, a wound is generated on a stem of a plant, growing point removal operation is carried out after true leaves grow out, and wound calluses are issued under the stimulation of hormone of the plant to breed into buds. The method is not limited to tissue culture and sterile operation, does not need root regeneration buds, does not need external application of hormone and does not need root regeneration induction, the operation steps are simple, and transgenic or gene edited plants can be obtained in a short time.